LI Zhao-jun, XIE Xiao-yu, ZHANG Shu-qing, LIANG Yong-chao
{"title":"Negative Effects of Oxytetracycline on Wheat (Triticum aestivum L.) Growth, Root Activity, Photosynthesis, and Chlorophyll Contents","authors":"LI Zhao-jun, XIE Xiao-yu, ZHANG Shu-qing, LIANG Yong-chao","doi":"10.1016/S1671-2927(11)60150-8","DOIUrl":"10.1016/S1671-2927(11)60150-8","url":null,"abstract":"<div><h3>Abstract</h3><p>A solution culture experiment was performed to investigate the effects of oxytetracycline (OTC) on wheat (<em>Triticum aestivum</em> L.) growth, chlorophyll contents, and photosynthesis at five levels of 0, 10, 20, 40, and 80 mmol L-<sup>1</sup> OTC. OTC is toxic to wheat. The wheat growth, especially wheat root was significantly decreased. Further OTC also significantly decreased root activity, chlorophyll contents, and photosynthetic parameters except for intercellular C0<sub>2</sub> concentrations. The different responses of indicators such as root number, root activity and so on to OTC were also observed. The IC<sub>50</sub> values for the tested indicators to OTC ranged from 7.1 to 113.4 mmol L-<sup>1</sup> OTC. The order of indicator sensitivity to OTC was root number > stomatal conductance > chlorophyll <em>a ></em> total chlorophyll >photosynthetic rates > total surface area > transpiration rate >chlorophyll <em>b</em>>fresh weight of root>dry weight of root>total length>dry weight of shoot = fresh weight of shoot>total volume. The root number was more sensitive than other indicators with the IC<sub>50</sub> value of 7.1 mmol L-<sup>1</sup> OTC, and could be taken as the sensitive indicator to predict the hazards of OTC to wheat.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1545-1553"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60150-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LI Rui , LI Cun-jun , DONG Ying-ying , LIU Feng , WANG Ji-hua , YANG Xiao-dong , PAN Yu-chun
{"title":"Assimilation of Remote Sensing and Crop Model for LAI Estimation Based on Ensemble Kaiman Filter","authors":"LI Rui , LI Cun-jun , DONG Ying-ying , LIU Feng , WANG Ji-hua , YANG Xiao-dong , PAN Yu-chun","doi":"10.1016/S1671-2927(11)60156-9","DOIUrl":"10.1016/S1671-2927(11)60156-9","url":null,"abstract":"<div><h3>Abstract</h3><p>Data assimilation in agricultural remote sensing research is of great significance to integrate with remote sensing observations and model simulations for parameters estimation. The present investigation not only designed and realized the Ensemble Kaiman Filtering algorithm (EnKF) assimilation by combing the crop growth model (CERES-Wheat) with remote sensing data, but also optimized and updated the key parameters (LAI) of winter wheat by using remote sensing data. Results showed that the assimilation LAI and the observation ones agreed with each other, and the <em>R<sup>2</sup></em> reached 0.8315. So assimilation remote sensing and crop model could provide reference data for the agricultural production.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1595-1602"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60156-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on Wool-Eating Ailment in Guizhou Semi-Fine Wool Sheep","authors":"SHEN Xiao-yun","doi":"10.1016/S1671-2927(11)60159-4","DOIUrl":"10.1016/S1671-2927(11)60159-4","url":null,"abstract":"<div><h3>Abstract</h3><p>The clinical symptoms of a disorder which is known locally as “wool-eating ailment” in Guizhou semi-fine wool sheep in the Weining County of Guizhou Province, China, are poor weight gain, lost appetite, pica, emaciation, and wool-eating. The mineral composition of forage, and samples of blood, liver and wool from Guizhou semi-fine wool sheep in affected ranches were compared with those from the unaffected areas. The mean concentration of iron in forage from affected pasture was significantly higher than that from the unaffected pasture. The mean concentration of sulfur in forage from the affected and unaffected pasture was similar and within normal ranges. The mean concentration of sulfur in blood, liver and wool from the affected Guizhou semi-fine wool sheep was significantly lower than that from the unaffected sheep while the content of iron in blood, liver and wool from the affected sheep was significantly higher than that from the unaffected sheep. Oral administration of calcium sulfate prevented and cured the disease. We concluded that the disease of Guizhou semi-fine wool sheep in the Weining County is due to S deficiency caused by the high Fe in forages.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1618-1623"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60159-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Variability of Pesticide Residues in Vegetables from the Marketplaces in Jinan City","authors":"SONG Yu-feng , LU Xiao , REN Feng-shan","doi":"10.1016/S1671-2927(11)60163-6","DOIUrl":"10.1016/S1671-2927(11)60163-6","url":null,"abstract":"<div><h3>Abstract</h3><p>In order to provide technological support for risk estimation of pesticide residues taken from vegetables, the residues of pesticide in vegetables in Jinan City, China, were investigated. Moreover, the variability of pesticide residues in vegetables was explored, and the variability factor was calculated. The level of pesticide residue was studied by statistical method. The variability factor was calculated using the Harrell-Davis method in accordance with principles recommended by the JMPR. In addition, the procedure of evaluation was validated according to the principles laid down by the JMPR (Joint Meeting on Pesticide Residues). The detection rate of pesticide residues in all marketplace samples was less than 20% in Jinan City. The unqualified rate of vegetables was low (0.6%). The chlorothalonil was found as the pesticide with the highest rate of detection. The vegetables with the highest rate of pesticide residues detected were leafy vegetables. The relationship between variability factor and mean value of concentrations of contaminations was expressed by the formula,\u0000<span><math><mrow><mi>v</mi><mo>=</mo><mn>4.9</mn><mi></mi><mo>×</mo><mi></mi><msubsup><mi>C</mi><mrow><mi>m</mi><mi>e</mi><mi>a</mi><msup><mi>n</mi><mo>*</mo></msup></mrow><mrow><mn>0.15</mn></mrow></msubsup></mrow></math></span>. The pesticide residues in vegetables from Jinan City is low. The evaluated variability factor is larger than that the value recommended by the JMPR, however, less than the default value used for acute intake assessment of pesticide residues in UK in most cases.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1646-1652"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60163-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
YU Zhong-dong , PENG Shao-bing , REN Zheng-zheng , WANG Dong-mei , CAO Zhi-min
{"title":"Infection Behaviour of Melampsora larici-populina on the Leaf Surface of Populus purdomii","authors":"YU Zhong-dong , PENG Shao-bing , REN Zheng-zheng , WANG Dong-mei , CAO Zhi-min","doi":"10.1016/S1671-2927(11)60152-1","DOIUrl":"10.1016/S1671-2927(11)60152-1","url":null,"abstract":"<div><h3>Abstract</h3><p>Behaviours of urediospore germtube <em>in Melampsora larici-populina</em> on the leaf surface of <em>Populus purdomii</em> were studied by light microscope, scanning electron microscope (SEM), transmission electron microscope (TEM), and fluorescence microscope. Crab-like fusion cells on leaf surface, intercellular hyphal cells in leaf tissues, as well as nucleus states, were observed and counted up in this study. Under unsaturated humidity, 32% of germinated tubes fused into a distinguishable swollen crab-shaped cell at the merging site, and 10.5% of observed crab-like cells had more than three nuclei. Wedge-shaped mycelia developed and then penetrated the leaf surface directly, or indirectly through stomata. Tips of germtube passed through the intercellular cells of poplar leaves directly were found in TEM. Aniline blue dyeing also showed that the infecting hyphae could invade into the cuticle and epidemic cell wall directly. For the case of infection through stomata, there were two different situations. Short branches and wedge hyphae usually penetrated the leaf surface <em>via</em> opened stomata, whereas, some germtube branches and wedge hyphae penetrated leaves through the guard cell walls or stoma lips. In the latter case, the stomata were always closed. The samples from wild forestlands had the same fused cells and wedge hyphae, but the occurrence rate was much higher than that in the chamber. Even under the saturated air humidity, germtubes could roll back and formed fusion structure, or merged together with their tips. The fusion cells might centralize the plasma of merged germtubes and have a strong survival capacity to protect germtubes from dying under arid circumstances, and provide a chance of genetic variation as well.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1562-1569"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60152-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
WU Xiao-ling , ZHANG Bao-qiang , SUN Shi , ZHAO Jin-ming , YANG Feng , GUO Na , GAI Jun-yi , XING Han
{"title":"Identification, Genetic Analysis and Mapping of Resistance to Phytophthora sojae of Pm28 in Soybean","authors":"WU Xiao-ling , ZHANG Bao-qiang , SUN Shi , ZHAO Jin-ming , YANG Feng , GUO Na , GAI Jun-yi , XING Han","doi":"10.1016/S1671-2927(11)60145-4","DOIUrl":"10.1016/S1671-2927(11)60145-4","url":null,"abstract":"<div><h3>Abstract</h3><p><em>Phytophthora sojae</em> Kanfman and Gerdemann <em>(P. sojae)</em> is one of the most prevalent pathogens and causes Phytophthora root rot, which limits soybean production worldwide. Development of resistant cultivars is a cost-effective approach to controlling this disease. In this study, 127 soybean germplasm were evaluated for their responses to <em>Phytophthora sojae</em> strain Pm28 using the hypocotyl inoculation technique, and 49 were found resistant to the strain. The hypocotyl of Pj, P<sub>2</sub>, Fj, and F<sub>23</sub> of two crosses of Ludou 4 (resistant) × Youchu 4 (susceptible) and Cangdou 5 (resistant) × Williams (susceptible) were inoculated with Pm28, and were used to analyze the inheritance of resistance. The population derived from the cross of Ludou 4 × Youchu 4 was used to map the resistance gene (designated as <em>Rps9)</em> to a linkage group. 932 pairs of SSR primers were used to detect polymorphism, and seven SSR markers were mapped near the resistance gene. The results showed that the resistance to Pm28 in Ludou 4 and Cangdou 5 was controlled by a single dominant gene <em>Rps9,</em> which was located on the molecular linkage group N between the SSR markers Satt631 (7.5 cM) and Sat_186 (4.3 cM).</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1506-1511"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60145-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of Genetic Effects for Heterosis of ErucicAcid and Glucosinolate Contents in Rapeseed (Brassica napus L.)","authors":"ZHANG Hai-zhen , SHI Chun-hai , WU Jian-guo","doi":"10.1016/S1671-2927(11)60147-8","DOIUrl":"10.1016/S1671-2927(11)60147-8","url":null,"abstract":"<div><h3>Abstract</h3><p>The embryo, cytoplasmic, and maternal heterosis for erucic acid content (EAC) and glucosinolate content (GLS) of rapeseed (<em>Brassica napus</em> L.) were studied by using the genetic models for quantitative traits of seeds in diploid crops. Eight parents were included in a diallel mating design in two years. It was found that the heterosis of EAC and GLS was simultaneously controlled by genetic main effects and genotypexenvironment (GE) interaction effects. The general heterosis of most crosses for EAC was significantly positive, while it was not for GLS. The general heterosis was more important for two quality traits of rapeseed because of the low GE interaction heterosis in both years, especially for GLS. Among different genetic systems, significant positive embryo general heterosis and the negative maternal general heterosis were found for EAC and GLS in most hybrid crosses. Some hybrids with significant negative interaction heterosis were detected for either of EAC or GLS. In general, maternal general and interaction heterosis was more important for reducing EAC and GLS of rapeseed.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1525-1531"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60147-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies of GnRH-A Active Immunization Effects on LH and FSH Secretion and Histostructure of the Ovary and Uterus in Rabbits","authors":"WEI Suo-cheng , GONG Zhuan-di , WEI Min","doi":"10.1016/S1671-2927(11)60161-2","DOIUrl":"10.1016/S1671-2927(11)60161-2","url":null,"abstract":"<div><h3>Abstract</h3><p>The objective of the study is to investigate the effects of active immunization against gonadotropin releasing hormone agonist (GnRH-A) on secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the pituitary, and to observe the histological structures and development about ovaries and uteri in female rabbits. 24 female rabbits <em>(Oryctolagus cuniculus)</em> were divided randomly into 4 groups (n=6), namely, experimental group I (EG-I), experimental II (EG-II), experimental III (EG-III), and control group (CG). Rabbits were subcutaneously injected with 1.0 mL GnRH-A (alarelin) antigen respectively at concentrations of 100, 100 and 50 ug mL”<sup>1</sup> respectively, in EG-I, EG-II and EG-III. Alarelin antigen was re-injected in EG-II and EG-III with the same dosage on 20 d. CG was a blank. The ovarian and uterine samples were collected aseptically at the end of the experiment of 70 d. The tissue slices were observed under light and electron microscopes. Serum concentrations of LH and FSH were measured with ELISA. The results showed that serum LH concentrations in EG-II and EG-III reached the peak levels on 50 and 40 d respectively, and LH level in EG-II exceeded other 3 groups on 50 d (i<>0.05). FSH level in EG-II was higher than those in EG-I, CG (PO.01) and EG-III (PO.05) on 40 d. GnRH-A could increase the number of primary follicles, enlarge the primary follicle vertical diameter (PFV) and primary follicle transverse diameter (PFT), and promote growth and maturation of follicles. The endometrial epithelium thickness (EET) and uterine wall thickness (UWT) in three EGs were less than that in CG (P<0.05). GnRH-A can increase the quantities of mitochondrial cristaes, cortex granules in cytoplasm, broaden and lengthen zona pellucidas and microvilli of oocytes. It also enlarged nuclei of ooxytes and mitochondria, thereby it promoted the development of oocytes. Re-injection of 100 ug alarelin antigen enhanced the secretion of LH and FSH. GnRH-A promoted the growth and maturation of ovaries and follicles, suppressed uterine development, and also influenced histostructure of ovaries and uteri in female rabbits.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1630-1637"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60161-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HUI Wei , NIU Rui-xue , SONG Yao-qiang , LI De-ying
{"title":"Inhibitory Effects of 1-MCP and DPA on Superficial Scald of Dangshansuli Pear","authors":"HUI Wei , NIU Rui-xue , SONG Yao-qiang , LI De-ying","doi":"10.1016/S1671-2927(11)60162-4","DOIUrl":"10.1016/S1671-2927(11)60162-4","url":null,"abstract":"<div><h3>Abstract</h3><p>Dangshansuli pear is one of crisp pears and has a non-climacteric respiratory pattern. Dangshansuli pear fruits were treated with 1 000 or 2 000 uL L-<sup>1</sup> diphenylamine (DPA) for 1 min, or with 0.5 uL L-<sup>1</sup> 1-methylcyclopropene (1-MCP) for 12 h before cold storage at (2±0.5)°C for up to 210 d. Incidences of superficial scald and related physiological indexes were investigated during storage. The results showed that superficial scald occurred in control fruit with a rate of over 90% after 210 d at cold storage plus 10 d at room temperature. Treatments with 1 000 and 2 000 uL L-<sup>1</sup> DPA and 0.5 uL L-<sup>1</sup>1-MCP inhibited the accumulation of a-farnesene, conjugated trienes, total phenolic contents, and malondialdehyde (MDA) during storage. The treatments also decreased leakage of cell membrane and the activity of polyphenol oxidase (PPO) in the peel. These results indicate that 1-MCP and DPA treatments inhibit and postpone the occurrence of superficial scald of Dangshansuli pears remarkably in cold storage and post-storage shelf life, respectively, while the mechanisms were different. The results above may provide a theoretical evidence for the agriculture production.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1638-1645"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60162-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mapping of Mutant Gene prbs Controlling Poly-Row-and-Branched Spike in Barley (Hordeum vulgare L.)","authors":"HUANG Bi-guang, WU Wei-ren","doi":"10.1016/S1671-2927(11)60144-2","DOIUrl":"10.1016/S1671-2927(11)60144-2","url":null,"abstract":"<div><h3>Abstract</h3><p>A row-type mutant of barley named poly-row-and-branched spike <em>(prbs)</em> was previously obtained from a two-rowed cultivar Pudamai-2 after treated by inflorescence soaking in maize total DNA solution. The mutant produces branched spikes with irregular multiple rows. Genetic analysis indicated that the mutant phenotype was caused by a recessive gene <em>prbs,</em> and the <em>PRBS</em> locus had a recessive epistatic effect on an independent locus (denoted as <em>Vrsx)</em> conferring the variation of two-rowed spike <em>vs.</em> six-rowed spike. This study aimed to map <em>PRBS</em> as well as <em>Vrsx</em> using simple sequence repeats (SSR) markers. We developed an F<sub>2</sub> population from a cross between the <em>prbs</em> mutant and a six-rowed cultivar Putianwudu for the gene mapping. As the two target loci interacted to result in a segregation ratio of two-rowed type:six-rowed <em>type:prbs=9:3:4</em> in the population, we adopted a special strategy to map the two loci. <em>PRBS</em> was mapped between SSR markers HvLTPPB and Bmag0508A on the short arm of chromosome 3H, with distances of 24.7 and 14.3 cM to the two markers, respectively. <em>Vrsx</em> was mapped between SSR markers Bmag0125 and Bmag0378 on chromosome 2H, with distances of 6.9 and 15.3 cM to the two markers, respectively. This suggests that <em>Vrsx</em> should be the known locus <em>Vrsl,</em> which predominantly controls row-type variation in barley cultivars, and <em>PRBS</em> is a new locus related to the row type of spikes in barley.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1501-1505"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60144-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}