Revue francophone des laboratoires : RFL最新文献

{"title":"Agrégométrie optique pour l’exploration des fonctions plaquettaires : synthèse des recommandations et propositions pour l’accréditation des examens","authors":"Alain Stépanian","doi":"10.1016/S1773-035X(26)76752-6","DOIUrl":"10.1016/S1773-035X(26)76752-6","url":null,"abstract":"<div><h3>Résumé</h3><div>L’agrégométrie optique est une méthode qui mesure l’augmentation de la transmission lumineuse d’un plasma riche en plaquettes (PRP) agité à 37 °C après ajout d’un agoniste. Elle est utilisée pour effectuer différents examens biologiques, dont l’exploration des fonctions plaquettaires in vitro dans l’exploration des syndromes hémorragiques. Cette méthode présente des spécificités liées à la nature de la matrice utilisée (plasma riche plaquettes, PRP) dont il faut tenir compte pour l’accréditation selon la norme NF EN ISO 15189. Après une enquête de pratiques réalisée auprès des membres de la Société française de thrombose et d’hémostase (SFTH), le groupe qualité de ce dernier a proposé une synthèse des différentes recommandations en cours et a formulé 28 propositions, qui couvrent les aspects pré-analytiques, analytiques et post-analytiques pour l‘accréditation des examens utilisant l’agrégométrie optique. Ces propositions ont été approuvées par le Centre de référence des pathologies plaquettaires constitutionnelles et par les membres de la SFTH après un vote. Elles sont destinées à guider la constitution des dossiers de validation de méthodes pour l’exploration des fonctions plaquettaires en PRP dans un contexte de recherche de thrombopathie et pour le test RIPA <em>(Ristocetininduced platelet aggregation)</em>. La plupart d’entre elles peuvent néanmoins être utilisées pour tous les autres examens utilisant cette méthode (par exemple, les tests fonctionnels pour le diagnostic de thrombopénie induite par l’héparine). Ce texte constitue un résumé de ces propositions.</div></div><div><h3>Abstract</h3><div>Light transmission aggregometry is a method that measures the increase in light transmission of a platelet-rich plasma (PRP) stirred at 37°C after the addition of an agonist. It is used to perform various biological tests, including the in vitro assessment of platelet function for the investigation of bleeding disorders. This method has specific characteristics related to the nature of the matrix used (platelet-rich plasma, PRP) that must be considered for accreditation according to the NF EN ISO 15189 standard. Following a survey of practices conducted among members of the Societe Francaise de Thrombose et d’Hemostase (SFTH), the society’s quality group proposed a synthesis of the various current recommendations and formulated 28 proposals covering pre-analytical, analytical, and post-analytical aspects for the accreditation of tests using optical aggregometry.</div><div>These proposals were approved by the Reference Center for Inherited Platelet Disorders and by the members of the SFTH following a vote. They are intended to provide guidance on the production of method validation files for the investigation of platelet functions in PRP in the context of platelet function disorder screening and for the RIPA (Ristocetin-induced platelet aggregation) test. Most of them can nevertheless be used for all other tests that employ ","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 585","pages":"Pages 55-59"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149096887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Le grand livre de l’arthrose","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(26)76741-1","DOIUrl":"10.1016/S1773-035X(26)76741-1","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 585","pages":"Page 12"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149096884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mauvaise graine dans la moelle","authors":"Estelle Fleuriot, Quentin Amiot, Hélène Vanquaethem, Audrey Wolf, Déborah Delaune, Laurie Poleszczuk, Aurore Bousquet","doi":"10.1016/S1773-035X(26)76755-1","DOIUrl":"10.1016/S1773-035X(26)76755-1","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 585","pages":"Pages 72-75"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149096889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Les thrombopénies constitutionnelles : classifications et diagnostic","authors":"Charlotte Holuigue,&nbsp;Anaëlle De Wreede,&nbsp;Sandrine Girard,&nbsp;Sophie Voisin,&nbsp;Agnès Ribes,&nbsp;Véronique Baccini","doi":"10.1016/S1773-035X(26)76748-4","DOIUrl":"10.1016/S1773-035X(26)76748-4","url":null,"abstract":"<div><h3>Résumé</h3><div>Les thrombopénies constitutionnelles (TC) représentent un groupe hétérogène d’affections rares caractérisées par une diminution chronique du nombre de plaquettes liée à une anomalie génétique affectant la production, la structure et/ou la fonction plaquettaires. Leur caractérisation s’est améliorée ces dernières années grâce aux progrès des techniques moléculaires, notamment le séquençage à haut débit (NGS). Leur diagnostic repose sur une approche intégrée combinant morphologie plaquettaire, exploration fonctionnelle (agrégométrie), phénotypique (cytométrie en flux), structurale (microscopie électronique) et analyse moléculaire. L’enjeu est double : réduire les errances et les erreurs diagnostiques (notamment les confusions avec une thrombopénie immunitaire) et identifier les formes syndromiques ou celles associées à un risque leucémogène ou de fibrose médullaire. Cet article propose une synthèse des principales thrombopénies constitutionnelles connues et présente une démarche diagnostique, incluant des arbres décisionnels afin de faciliter l’orientation diagnostique vers une cause constitutionnelle avant d’adresser les patients vers un centre spécialisé en lien avec le Centre de référence des pathologies plaquettaires (CRPP).</div></div><div><h3>Abstract</h3><div>Inherited thrombocytopenias represent a heterogeneous group of rare diseases characterized by a chronic decrease in platelet count related to a genetic abnormality affecting platelet production, structure and/or function. Their characterization has improved in recent years thanks to advances in molecular techniques, notably next-generation sequencing (NGS). Diagnosis is based on an integrated approach combining platelet morphology, functional exploration (aggregometry), phenotypic (flow cytometry), structural (electron microscopy) and molecular analysis. The challenge is twofold: to reduce diagnostic delay and errors (particularly confusions with an immune thrombocytopenia) and to identify forms associated with a syndromic features or hematological risk. This article provides an overview of the main inherited thrombocytopenias and presents a diagnostic approach, including decision trees to facilitate orientation towards a constitutional cause before referral to a specialized center linked to the french Reference Center for constitutive Platelet Disorders.</div></div>","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 585","pages":"Pages 16-29"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149096897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Un myélome multiple atypique à ne pas prendre à la légère !","authors":"Marine Jaillard,&nbsp;Julie Milandri,&nbsp;Magali Dechomet","doi":"10.1016/S1773-035X(26)76726-5","DOIUrl":"10.1016/S1773-035X(26)76726-5","url":null,"abstract":"<div><h3>Résumé</h3><div>Le myélome multiple (MM) à chaînes légères libres est une gammapathie monoclonale représentant 15 % des myélomes multiples. Les plus fréquents sont les myélomes à IgG (56 %) et les myélomes à IgA (26 %). L’âge moyen au diagnostic est de 65 ans. D’après la littérature, seulement 2 % des cas sont des patients de moins de 40 ans et 0,2 % des cas sont des patients de moins de 30 ans. Ce cas retrace l’histoire d’un patient de 29 ans, atteint d’un myélome à chaînes légères libres kappa à présentation atypique. Le diagnostic a été réalisé aux urgences, après adressage par le médecin traitant, et le patient a été pris en charge sans délai dans le service d’hématologie clinique.</div></div><div><h3>Abstract</h3><div>Free light chain multiple myeloma (MM) is a monoclonal gammopathy representing 15 % of multiple myelomas. The most common are IgG myelomas (56 %) and IgA myelomas (26 %). The average age at diagnosis is around 65 years. According to the literature, only 2 % of cases are patients aged under 40 and 0,2 % of cases are patients under 30. We report the case of a 29-year-old patient diagnosed with atypical kappa free light chain myeloma. The diagnosis was established in the emergency department following referral by the general practitioner, and the patient was immediately managed to the clinical hematology department for treatment.</div></div>","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 584","pages":"Pages 50-55"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148660967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fin de vie: Manuel de résistance","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(26)76709-5","DOIUrl":"10.1016/S1773-035X(26)76709-5","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 584","pages":"Page 12"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148661224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Textes juridiques","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(26)76711-3","DOIUrl":"10.1016/S1773-035X(26)76711-3","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 584","pages":"Page 13"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148661226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lymphome T associé à une entéropathie (EATL) : une entité rare au croisement de l’immunologie et de l’hématologie","authors":"Gianni Di Vittorio,&nbsp;Leslie Bathomen,&nbsp;Anaïs Nombel,&nbsp;Lorna Garnier","doi":"10.1016/S1773-035X(26)76729-0","DOIUrl":"10.1016/S1773-035X(26)76729-0","url":null,"abstract":"<div><h3>Résumé</h3><div>La maladie coeliaque (MC) est une entéropathie auto-immune pouvant se compliquer en lymphome T associé à une entéropathie (EATL pour <em>Enteropathy-associated T-cell lymphoma</em>), notamment en cas de diagnostic tardif (forme paucisymptomatique), de forme réfractaire ou de mauvaise observance du régime sans gluten (RSG). Nous rapportons le cas d’un homme de 54 ans présentant une altération de l’état général avec amaigrissement, anomalies hépatiques et cytopénies, sans symptômes digestifs évocateurs. Une maladie coeliaque est ensuite diagnostiquée sur des arguments biologiques et histologiques. Dans ce contexte de maladie coeliaque diagnostiquée tardivement d’infiltration médullaire lymphocytaire monomorphe, le diagnostic d’EATL est retenu.</div></div><div><h3>Abstract</h3><div>Celiac disease is an autoimmune enteropathy that may be complicated by enteropathy-associated T-cell lymphoma (EATL), particularly in cases of latediagnosis (pauci-symptomatic form), or refractory form, or poor adherence to a gluten-free diet. We report the case of a 54-year-old man who presented with impaired of general condition with weight loss, abnormal liver function and cytopenias, without any suggestive gastrointestinal symptoms. Celiac disease was subsequently diagnosed based on biological and histological findings. In the context of late-diagnosed celiac disease, and monomorphic lymphocytic bone marrow infiltration, a diagnosis of EATL was made.</div></div>","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 584","pages":"Pages 65-69"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148661313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthrite septique à Yersinia enterocolitica sur prothèse totale de hanche, à propos d’un cas et revue de la littérature","authors":"Albane Karkulowski,&nbsp;Asma Zribi,&nbsp;Camille Courboulès,&nbsp;Anne-Laure Roux","doi":"10.1016/S1773-035X(26)76730-7","DOIUrl":"10.1016/S1773-035X(26)76730-7","url":null,"abstract":"<div><h3>Résumé</h3><div>Nous rapportons un cas d’infection sur prothèse de hanche à <em>Yersinia enterocolitica</em> chez un patient ne présentant aucun facteur de risque. Ce cas est complété par une revue de la littérature des cas d’infections ostéo-articulaires sur matériel à <em>Yersinia enterocolitica</em> qui restent une affection rare.</div></div><div><h3>Abstract</h3><div>We report a case of hip prosthesis infection caused by <em>Yersinia enterocolitica</em> in a patient with no risk factors. We also conducted a literature review of osteoarticular infections involving prosthetic material due to <em>Yersinia enterocolitica</em>, which remain a rare condition.</div></div>","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 584","pages":"Pages 70-74"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148661314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ce que l’on sait du hantavirus…: et ce qu’il faut en faire","authors":"Jean-Marie Manus","doi":"10.1016/S1773-035X(26)76702-2","DOIUrl":"10.1016/S1773-035X(26)76702-2","url":null,"abstract":"","PeriodicalId":74728,"journal":{"name":"Revue francophone des laboratoires : RFL","volume":"2026 584","pages":"Pages 6-7"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148661368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}