Osteoarthritis and cartilage open最新文献

{"title":"Effects of self-assembled type II collagen fibrils on the morphology and growth of pre-chondrogenic ATDC5 cells","authors":"Linyan Shi ,&nbsp;Kazuhiro Ura ,&nbsp;Yasuaki Takagi","doi":"10.1016/j.ocarto.2024.100450","DOIUrl":"https://doi.org/10.1016/j.ocarto.2024.100450","url":null,"abstract":"<div><h3>Objective</h3><p>Although type II collagen could have marked potential for developing cartilage tissue engineering (CTE) scaffolds, its erratic supply and viscous nature have limited these studies, and there are no studies on the use of marine-derived type II collagen fibrils for CTE scaffold materials. In this study, we aimed to generate a fibril-based, thin-layered scaffold from marine-derived type II collagen and investigate its chondrogenic potential.</p></div><div><h3>Methods</h3><p>Time-lapse observations revealed the cell adhesion process. The Cell Counting Kit-8 (CCK-8) assay, light microscopy, and scanning electron microscopy were performed to detect proliferation and filopodium morphology. Alcian blue staining was used to show the deposition of extracellular secretions, and qRT-PCR was performed to reveal the expression levels of chondrogenesis-related genes.</p></div><div><h3>Results</h3><p>The cell adhesion speed was similar in both fibril-coated and control molecule-coated groups, but the cellular morphology, proliferation, and chondrogenesis activity differed. On fibrils, more elongated finer filopodia showed inter-cell communications, whereas the slower proliferation suggested an altered cell cycle. Extracellular secretions occurred before day 14 and continued until day 28 on fibrils, and on fibrils, the expression of the chondrogenesis-related genes <em>Sox9</em> (<em>p ​&lt; ​0.001</em>), <em>Col10a1</em> (<em>p ​&lt; ​0.001</em>), <em>Acan</em> (<em>p ​&lt; ​0.001</em>), and <em>Col2a1</em> (<em>p</em> ​= ​0.0049) was significantly upregulated on day 21.</p></div><div><h3>Conclusion</h3><p>Marine-derived type II collagen was, for the first time, fabricated into a fibril state. It showed rapid cellular affinity and induced chondrogenesis with extracellular secretions. We presented a new model for studying chondrogenesis <em>in vitro</em> and a potential alternative material for cell-laden CTE research.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 2","pages":"Article 100450"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000177/pdfft?md5=9786c6446d2842bf1cfd68c92ffdeac7&pid=1-s2.0-S2665913124000177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140015222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Effect of exercise therapy in patients with hip osteoarthritis: A systematic review and cumulative meta-analysis” [Osteoarthr Cartil Open 5 (2023) 100338]","authors":"Carolien H. Teirlinck ,&nbsp;Arianne P. Verhagen ,&nbsp;Leontien M. van Ravesteyn ,&nbsp;Elja A.E. Reijneveld-van de Vendel ,&nbsp;Jos Runhaar ,&nbsp;Marienke van Middelkoop ,&nbsp;Manuela L. Ferreira ,&nbsp;Sita MA. Bierma-Zeinstra","doi":"10.1016/j.ocarto.2024.100447","DOIUrl":"https://doi.org/10.1016/j.ocarto.2024.100447","url":null,"abstract":"","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 2","pages":"Article 100447"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000141/pdfft?md5=b18d4a0171d3a965fd7389bae642f6fe&pid=1-s2.0-S2665913124000141-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139985940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiomics analysis of patellofemoral joint improves knee replacement risk prediction: Data from the Multicenter Osteoarthritis Study (MOST)","authors":"Jiang Zhang ,&nbsp;Tianshu Jiang ,&nbsp;Lok-Chun Chan ,&nbsp;Sing-Hin Lau ,&nbsp;Wei Wang ,&nbsp;Xinzhi Teng ,&nbsp;Ping-Keung Chan ,&nbsp;Jing Cai ,&nbsp;Chunyi Wen","doi":"10.1016/j.ocarto.2024.100448","DOIUrl":"https://doi.org/10.1016/j.ocarto.2024.100448","url":null,"abstract":"<div><h3>Objective</h3><p>Knee replacement (KR) is the last-resort treatment for knee osteoarthritis. Although radiographic evidence of tibiofemoral joint has been widely adopted for prognostication, patellofemoral joint has gained little attention and may hold additional value for further improvements. We aimed to quantitatively analyse patellofemoral joint through radiomics analysis of lateral view radiographs for improved KR risk prediction.</p></div><div><h3>Design</h3><p>From the Multicenter Osteoarthritis Study dataset, we retrospectively retrieved the initial-visit lateral left knee radiographs of 2943 patients aged 50 to 79. They were split into training and test cohorts at a 2:1 ratio. A comprehensive set of radiomic features were extracted within the best-performing subregion of patellofemoral joint and combined into a radiomics score (RadScore). A KR risk score, derived from Kellgren-Lawrence grade (KLG) of tibiofemoral joint and RadScore of patellofemoral joint, was developed by multivariate Cox regression and assessed using time-dependent area under receiver operating characteristic curve (AUC).</p></div><div><h3>Results</h3><p>While patellofemoral osteoarthritis (PFOA) was insignificant during multivariate analysis, RadScore was identified as an independent risk factor (multivariate Cox p-value &lt; 0.001) for KR. The subgroup analysis revealed that RadScore was particularly effective in predicting rapid progressor (KR occurrence before 30 months) among early- (KLG &lt; 2) and mid-stage (KLG ​= ​2) patients. Combining two joints radiographic information, the AUC reached 0.89/0.87 for predicting 60-month KR occurrence.</p></div><div><h3>Conclusions</h3><p>The RadScore of the patellofemoral joint on lateral radiographs emerges as an independent prognostic factor for improving KR prognosis prediction. The KR risk score could be instrumental in managing progressive knee osteoarthritis interventions.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 2","pages":"Article 100448"},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000153/pdfft?md5=1f28f1c6bae596eba49943d0058a2386&pid=1-s2.0-S2665913124000153-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139992979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward designing human intervention studies to prevent osteoarthritis after knee injury: A report from an interdisciplinary OARSI 2023 workshop","authors":"Jackie L. Whittaker ,&nbsp;Raneem Kalsoum ,&nbsp;James Bilzon ,&nbsp;Philip G. Conaghan ,&nbsp;Kay Crossley ,&nbsp;George R. Dodge ,&nbsp;Alan Getgood ,&nbsp;Xiaojuan Li ,&nbsp;Elena Losina ,&nbsp;Deborah J. Mason ,&nbsp;Brian Pietrosimone ,&nbsp;May Arna Risberg ,&nbsp;Frank Roemer ,&nbsp;David Felson ,&nbsp;Adam G. Culvenor ,&nbsp;Duncan Meuffels ,&nbsp;Nicole Gerwin ,&nbsp;Lee S. Simon ,&nbsp;L. Stefan Lohmander ,&nbsp;Martin Englund ,&nbsp;Fiona E. Watt","doi":"10.1016/j.ocarto.2024.100449","DOIUrl":"https://doi.org/10.1016/j.ocarto.2024.100449","url":null,"abstract":"<div><h3>Objective</h3><p>The global impact of osteoarthritis is growing. Currently no disease modifying osteoarthritis drugs/therapies exist, increasing the need for preventative strategies. Knee injuries have a high prevalence, distinct onset, and strong independent association with post-traumatic osteoarthritis (PTOA). Numerous groups are embarking upon research that will culminate in clinical trials to assess the effect of interventions to prevent knee PTOA despite challenges and lack of consensus about trial design in this population. Our objectives were to improve awareness of knee PTOA prevention trial design and discuss state-of-the art methods to address the unique opportunities and challenges of these studies.</p></div><div><h3>Design</h3><p>An international interdisciplinary group developed a workshop, hosted at the 2023 Osteoarthritis Research Society International Congress. Here we summarize the workshop content and outputs, with the goal of moving the field of PTOA prevention trial design forward.</p></div><div><h3>Results</h3><p>Workshop highlights included discussions about target population (considering risk, homogeneity, and possibility of modifying osteoarthritis outcome); target treatment (considering delivery, timing, feasibility and effectiveness); comparators (usual care, placebo), and primary symptomatic outcomes considering surrogates and the importance of knee function and symptoms other than pain to this population.</p></div><div><h3>Conclusions</h3><p>Opportunities to test multimodal PTOA prevention interventions across preclinical models and clinical trials exist. As improving symptomatic outcomes aligns with patient and regulator priorities, co-primary symptomatic (single or aggregate/multidimensional outcome considering function and symptoms beyond pain) and structural/physiological outcomes may be appropriate for these trials. To ensure PTOA prevention trials are relevant and acceptable to all stakeholders, future research should address critical knowledge gaps and challenges.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 2","pages":"Article 100449"},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000165/pdfft?md5=6f50c58e056dcf0e2bddb41e974a816e&pid=1-s2.0-S2665913124000165-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140000068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An interpretable knee replacement risk assessment system for osteoarthritis patients","authors":"H.H.T. Li ,&nbsp;L.C. Chan ,&nbsp;P.K. Chan ,&nbsp;C. Wen","doi":"10.1016/j.ocarto.2024.100440","DOIUrl":"https://doi.org/10.1016/j.ocarto.2024.100440","url":null,"abstract":"<div><h3>Objective</h3><p>Knee osteoarthritis (OA) is a complex disease with heterogeneous representations. Although it is modifiable to prevention and early treatment, there still lacks a reliable and accurate prognostic tool. Hence, we aim to develop a quantitative and self-administrable knee replacement (KR) risk stratification system for knee osteoarthritis (KOA) patients with clinical features.</p></div><div><h3>Method</h3><p>A total of 14 baseline features were extracted from 9592 cases in the Osteoarthritis Initiative (OAI) cohort. A survival model was constructed using the Random Survival Forests algorithm. The prediction performance was evaluated with the concordance index (C-index) and average receiver operating characteristic curve (AUC). A three-class KR risk stratification system was built to differentiate three distinct KR-free survival groups. Thereafter, Shapley Additive Explanations (SHAP) was introduced for model explanation.</p></div><div><h3>Results</h3><p>KR incidence was accurately predicted by the model with a C-index of 0.770 (±0.0215) and an average AUC of 0.807 (±0.0181) with 14 clinical features. Three distinct survival groups were observed from the ten-point KR risk stratification system with a four-year KR rate of 0.79%, 5.78%, and 16.2% from the low, medium, and high-risk groups respectively. KR is mainly caused by pain medication use, age, surgery history, diabetes, and a high body mass index, as revealed by SHAP.</p></div><div><h3>Conclusion</h3><p>A self-administrable and interpretable KR survival model was developed, underscoring a KR risk scoring system to stratify KOA patients. It will encourage regular self-assessments within the community and facilitate personalised healthcare for both primary and secondary prevention of KOA.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 2","pages":"Article 100440"},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000074/pdfft?md5=6c0ece314d89757e61fef33c4d75a21f&pid=1-s2.0-S2665913124000074-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139898680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity-related pain and sensitization predict within- and between-person pain experience in people with knee osteoarthritis: An ecological momentary assessment study","authors":"Mark Overton ,&nbsp;Nicola Swain ,&nbsp;Carrie Falling ,&nbsp;David Gwynne-Jones ,&nbsp;Roger Fillingim ,&nbsp;Ramakrishnan Mani","doi":"10.1016/j.ocarto.2024.100439","DOIUrl":"https://doi.org/10.1016/j.ocarto.2024.100439","url":null,"abstract":"<div><h3>Background and objectives</h3><p>Knee Osteoarthritis (OA) is a prevalent musculoskeletal condition that often results in pain and disability. Determining factors predicting variability in pain experience is critical to improving clinical outcomes. Underlying pain sensitization and its clinical manifestations, such as activity-related pain, may better predict the knee OA pain experience. This study aimed to determine whether Quantitative Sensory Testing (QST) derived sensitization measures and activity-related pain predict knee OA pain experiences collected via smartphone ecological momentary assessment (EMA).</p></div><div><h3>Design</h3><p>Individuals with knee OA were recruited from an urban community in New Zealand. Those eligible to participate underwent baseline QST with clinical measures of activity-related pain also being collected. The knee OA pain experience was collected via smartphone EMA three times daily for two weeks. Mixed effects location scale models were developed using a multilevel modelling approach.</p></div><div><h3>Results</h3><p>Eighty-six participants with knee OA participated in the study. Mean age was 67.3 years, with most of the participants being female (64%) and New Zealand European (90.6%). Activity-related pain predicted worse and more variable pain intensity, pain interference, and bothersomeness outcomes within and between individuals with knee OA. Widespread cold hyperalgesia and local mechanical hyperalgesia were shown to predict higher within-person variability in pain intensity and pain interference respectively, while mechanical temporal summation predicted less within-person variability in pain intensity and interference.</p></div><div><h3>Discussion</h3><p>Those demonstrating activity-related pain and sensitization could be at risk of experiencing worse and more variable knee OA pain in the subsequent weeks. Testing for sensitization in clinical practice could therefore identify those at greatest risk of higher and more variable knee OA pain experiences and in greatest need of treatment. Larger validation studies are required, which include individuals with more severe knee OA.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 1","pages":"Article 100439"},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000062/pdfft?md5=74f067882e11afd5381ecce306c3f1b1&pid=1-s2.0-S2665913124000062-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139744363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does sex affect the efficacy of systemic pharmacological treatments of pain in knee osteoarthritis? A systematic review","authors":"Santiago Espinosa-Salas ,&nbsp;Leila Bagherzadeh ,&nbsp;Q. Eileen Wafford ,&nbsp;Graeme Witte ,&nbsp;Thomas J. Schnitzer","doi":"10.1016/j.ocarto.2024.100438","DOIUrl":"https://doi.org/10.1016/j.ocarto.2024.100438","url":null,"abstract":"<div><h3>Objective</h3><p>To determine whether sex influences the analgesic efficacy of systemic pharmacological treatment in patients with knee osteoarthritis.</p></div><div><h3>Design</h3><p>A systematic review, guided by Cochrane methods, sourced studies from Medline, Cochrane Library, Embase, and CINAHL Plus with Full Text as of October 10, 2022. Eligible studies were double-blind RCTs evaluating systemic pharmacological treatments for knee osteoarthritis in adults, with minimum 30-day treatment duration, reporting sex-specific results or mentioning sex subgroup analysis for analgesic efficacy. The risk of bias was assessed using the Cochrane Risk of Bias tool version 2 (RoB 2).</p></div><div><h3>Results</h3><p>9 studies (5201 participants) met inclusion criteria, analyzing drugs including duloxetine, etoricoxib, tapentadol, naproxcinod, lutikizumab, and rofecoxib. Only one study reported sex-specific results. Review findings suggested no significant sex-based differences in treatment efficacy, however, data were limited due to a lack of sex-specific reporting or inclusion of sex in subgroup analyses.</p></div><div><h3>Conclusions</h3><p>Current evidence does not support the existence of sex differences in the analgesic efficacy of systemic knee osteoarthritis treatments. However, this conclusion is substantially limited by the paucity of sex-specific reporting of results or subgroup analyses in most primary studies, emphasizing the need for future research to report on sex-stratified data to allow for comprehensive, personalized treatment strategies.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 1","pages":"Article 100438"},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000050/pdfft?md5=f67a9f929d0ef976fa4b3560782ef35e&pid=1-s2.0-S2665913124000050-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex and age differences in the patient-reported outcome measures and adherence to an osteoarthritis digital self-management intervention","authors":"Simone Battista ,&nbsp;L Stefan Lohmander ,&nbsp;Andrea Dell’Isola ,&nbsp;Leif E. Dahlberg ,&nbsp;Ali Kiadaliri","doi":"10.1016/j.ocarto.2024.100437","DOIUrl":"10.1016/j.ocarto.2024.100437","url":null,"abstract":"<div><h3>Objective</h3><p>To explore sex and age differences in Patient-Reported Outcomes Measures (PROMs) and adherence to digital osteoarthritis (OA) self-management intervention.</p></div><div><h3>Methods</h3><p>A register-based study with data from an OA digital self-management intervention. PROMs and adherence were collected at baseline and/or 3 ​month follow-up: ‘pain intensity’ in hip/knee (best/worst: 0–10), ‘activity impairments' (best/worst: 0–10), ‘overall health’ perception (worst/best: 0–10), ‘physical function’ (30-s chair stand test), ‘health-related quality of life’ (EQ-5D-5L index score; worst/best: 0.243–0.976), the subscales and total scores of the Knee Injury/Hip Disability and Osteoarthritis Outcome Score (KOOS/HOOS-12; worst/best: 0–100), ‘fear of movement’ (yes/no), ‘walking difficulties' (yes/no), ‘programme adherence’ (0–100 ​% and ≥80 ​% [yes/no]), ‘patient acceptable symptom state’ (PASS; yes/no), and ‘treatment failure’ (those who answered no to PASS question and thought the treatment failed [yes/no]). We used linear/logistic regression to calculate mean/risk differences in the PROMs and adherence levels among sex and age groups at 3-month follow-up. We employed entropy balancing to explore the contributions of baseline characteristics and different covariates to the sex/age differences.</p></div><div><h3>Results</h3><p>We included 14,610 participants (mean (SD) age: 64.1 (9.1), 75.5 ​% females). Females generally reported better outcomes than males. Participants aged ≥70 had greater activity impairments, lower KOOS/HOOS-pain/function scores, more walking difficulties, less fear of movement and higher adherence than those &lt;70. However, these differences were small and not likely clinically relevant.</p></div><div><h3>Conclusion</h3><p>No clinically relevant differences in PROMs and adherence were found among sex/age groups in this digital OA programme, suggesting that sex/age seemed not to impact the outcomes of this intervention.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 1","pages":"Article 100437"},"PeriodicalIF":0.0,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000049/pdfft?md5=2d81268056e9a6efe8935ec2bb7367d7&pid=1-s2.0-S2665913124000049-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139639368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Energy Computed Tomography Cannot Effectively Differentiate Between Calcium Pyrophosphate and Basic Calcium Phosphate Diseases in the Clinical Setting","authors":"Mohamed Jarraya ,&nbsp;Olivier Bitoun ,&nbsp;Dufan Wu ,&nbsp;Rene Balza ,&nbsp;Ali Guermazi ,&nbsp;Jamie Collins ,&nbsp;Rajiv Gupta ,&nbsp;Gunnlaugur Petur Nielsen ,&nbsp;Elias Guermazi ,&nbsp;F. Joseph Simeone ,&nbsp;Patrick Omoumi ,&nbsp;Christopher M. Melnic ,&nbsp;Seonghwan Yee","doi":"10.1016/j.ocarto.2024.100436","DOIUrl":"10.1016/j.ocarto.2024.100436","url":null,"abstract":"<div><h3>Background</h3><p>Recent reports suggested that dual-energy CT (DECT) may help discriminate between different types of calcium phosphate crystals <em>in vivo</em>, which would have important implications for the characterization of crystal deposition occurring in osteoarthritis.</p></div><div><h3>Purpose</h3><p>Our aim was to test the hypothesis that DECT can effectively differentiate basic calcium phosphate (BCP) from calcium pyrophosphate (CPP) deposition diseases.</p></div><div><h3>Methods</h3><p>Discarded tissue after total knee replacement specimens in a 71year-old patient with knee osteoarthritis and chondrocalcinosis was scanned using DECT at standard clinical parameters. Specimens were then examined on light microscopy which revealed CPP deposition in 4 specimens (medial femoral condyle, lateral tibial plateau and both menisci) without BCP deposition. Regions of interest were placed on post-processed CT images using Rho/Z maps (Syngo.via, Siemens Healthineers, VB10B) in different areas of CPP deposition, trabecular bone BCP (T-BCP) and subchondral bone plate BCP (C-BCP).</p></div><div><h3>Results</h3><p>Dual Energy Index (DEI) of CPP was 0.12 (SD=0.02) for reader 1 and 0.09 (SD=0.03) for reader 2, The effective atomic number (Z_<em>eff</em>) of CPP was 10.83 (SD=0.44) for reader 1 and 10.11 (SD=0.66) for reader 2. Nearly all DECT parameters of CPP were higher than those of T-BCP, lower than those of C-BCP, and largely overlapping with Aggregate-BCP (aggregate of T-BCP and C-BCP).</p></div><div><h3>Conclusion</h3><p>Differentiation of different types of calcium crystals using DECT is not feasible in a clinical setting.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 1","pages":"Article 100436"},"PeriodicalIF":0.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000037/pdfft?md5=9d61c0aec17055802b00f66a6deb51f1&pid=1-s2.0-S2665913124000037-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139633055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low back pain patients with Modic type 1 changes exhibit distinct bacterial and non-bacterial subtypes","authors":"I. Heggli ,&nbsp;T. Mengis ,&nbsp;C.J. Laux ,&nbsp;L. Opitz ,&nbsp;N. Herger ,&nbsp;D. Menghini ,&nbsp;R. Schuepbach ,&nbsp;N.A. Farshad-Amacker ,&nbsp;F. Brunner ,&nbsp;A.J. Fields ,&nbsp;M. Farshad ,&nbsp;O. Distler ,&nbsp;S. Dudli","doi":"10.1016/j.ocarto.2024.100434","DOIUrl":"10.1016/j.ocarto.2024.100434","url":null,"abstract":"<div><h3>Objectives</h3><p>Modic type 1 changes (MC1) are vertebral endplate bone marrow (BM) lesions observed on magnetic resonance images in sub-populations of chronic low back pain (CLBP) patients. The etiopathogenesis remains unknown and treatments that modify the underlying pathomechanisms do not exist. We hypothesized that two biological MC1 subtypes exist: a bacterial and a non-bacterial. This would have important implications for developing treatments targeting the underlying pathomechanisms.</p></div><div><h3>Methods</h3><p>Intervertebral disc (IVD) samples adjacent to MC1 (n ​= ​34) and control (n ​= ​11) vertebrae were collected from patients undergoing spinal fusion. <em>Cutibacterium acnes</em> (<em>C.acnes</em>) genome copy numbers (GCNs) were quantified in IVD tissues with 16S qPCR, transcriptomic signatures and cytokine profiles were determined in MC1 and control BM by RNA sequencing and immunoassay. Finally, we assessed if <em>C.acnes</em> GCNs are associated with blood plasma cytokines.</p></div><div><h3>Results</h3><p>IVD tissues from control levels had &lt;870 ​<em>C.acnes</em> GCNs/gram IVD. MC1-adjacent IVDs had either “low” (&lt;870) or “high” (&gt;870) <em>C.acnes</em> GCNs. MC1 patients with “high” <em>C.acnes</em> GCNs had upregulated innate immune cell signatures (neutrophil, macrophage/monocyte) and pro-inflammatory cytokines related to neutrophil and macrophage/monocyte function in the BM, consistent with a host defense against bacterium. MC1 patients with “low” <em>C.acnes</em> GCNs had increased adaptive immune cell signatures (T-and B-cell) in the BM and elevated IL-13 blood plasma levels.</p></div><div><h3>Conclusion</h3><p>Our study provides the first evidence for the existence of bacterial (<em>C.acnes</em> “high”) and non-bacterial (<em>C.acnes</em> “low”) subtypes in MC1 patients with CLBP. This supports the need for different treatment strategies.</p></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 1","pages":"Article 100434"},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665913124000013/pdfft?md5=017ba253e253d2788d1a43967a3f5896&pid=1-s2.0-S2665913124000013-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139636068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}