Nature reviews bioengineering最新文献_第4页

Point-of-care tuberculosis testing 即时结核病检测

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-03 DOI: 10.1038/s44222-025-00327-0

Caroline Beyer

引用次数: 0

Oversimplified immunology is holding biomaterials back 过度简化的免疫学阻碍了生物材料的发展

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-19 DOI: 10.1038/s44222-025-00320-7

Jessica L. Stelzel, Jonathan P. Schneck, Hai-Quan Mao, Joshua C. Doloff

引用次数: 0

Protein design and optimization for synthetic cells 合成细胞的蛋白质设计与优化

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-19 DOI: 10.1038/s44222-025-00318-1

Béla P. Frohn, Shunshi Kohyama, Petra Schwille

{"title":"Protein design and optimization for synthetic cells","authors":"Béla P. Frohn, Shunshi Kohyama, Petra Schwille","doi":"10.1038/s44222-025-00318-1","DOIUrl":"10.1038/s44222-025-00318-1","url":null,"abstract":"Proteins are essential components in synthetic biology, providing multiple functions at the nanoscale. Newly developed protein optimization and design tools allow the generation of proteins with desired properties, offering new opportunities for the engineering of protein-based biological systems. In this Review, we explore how bottom-up synthetic biology, with its aim to construct synthetic cells, can use these tools to devise complex biological functions and functional systems from scratch. We provide an overview of current capabilities in protein optimization, de novo protein design and iterative system optimization, and discuss their potential in synthetic cell science with regard to standardization, the generation of missing functionality and integration. We conclude with the outline of an integrated pipeline that combines protein engineering, automated synthetic cell generation and active learning, which might allow the design of entirely new biological systems that do not rely on naturally evolved protein components. Bottom-up synthetic biology might greatly benefit from custom-made proteins as components of new biological systems. This Review discusses various protein optimization and design approaches, and explores how these can contribute to the generation of controllable synthetic cells.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 8","pages":"645-659"},"PeriodicalIF":37.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioengineering needs diversity 生物工程需要多样性

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-16 DOI: 10.1038/s44222-025-00317-2

引用次数: 0

CRISPR–Cas therapies targeting bacteria 靶向细菌的CRISPR-Cas疗法

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-13 DOI: 10.1038/s44222-025-00311-8

Fabienne Benz, Beatriz Beamud, Raphael Laurenceau, Amandine Maire, Xavier Duportet, Antoine Decrulle, David Bikard

{"title":"CRISPR–Cas therapies targeting bacteria","authors":"Fabienne Benz, Beatriz Beamud, Raphael Laurenceau, Amandine Maire, Xavier Duportet, Antoine Decrulle, David Bikard","doi":"10.1038/s44222-025-00311-8","DOIUrl":"10.1038/s44222-025-00311-8","url":null,"abstract":"Technologies derived from the CRISPR (clustered regularly interspaced short palindromic repeats)–Cas immune system of prokaryotes have revolutionized our ability to cleave and modify target nucleic acid sequences. In addition to the use of CRISPR–Cas tools for the editing of human genes, they can also be designed to target pathogenic and commensal bacteria that colonize the body, offering new pathways for the treatment of infections and microbiome modulation. In this Review, we explore how the CRISPR–Cas toolbox can be engineered to kill or modify specific bacteria. We discuss DNA-targeting and RNA-targeting strategies, outlining how these can be applied to disarm bacteria by removing, modifying or silencing specific genes. Furthermore, we examine the delivery of CRISPR–Cas tools by bacteriophages and through conjugation and explore intracellular barriers to CRISPR–Cas tool maintenance and expression. Finally, we highlight therapeutic opportunities in the treatment of infectious diseases and for the modification of the microbiome, outlining progress and challenges in translating these approaches into clinical applications. CRISPR–Cas tools can be designed to kill or modify specific bacteria. This Review explores the engineering of CRISPR–Cas tools and corresponding delivery strategies for the treatment of bacterial infections and modification of the microbiome.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 8","pages":"627-644"},"PeriodicalIF":37.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA–lipid nanoparticles for acute critical illnesses rna -脂质纳米颗粒用于急性危重疾病

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-12 DOI: 10.1038/s44222-025-00314-5

Serena Omo-Lamai, Marco E. Zamora, Bernhard K. Mueller, Jessica C. Casciano, Jacob S. Brenner

{"title":"RNA–lipid nanoparticles for acute critical illnesses","authors":"Serena Omo-Lamai, Marco E. Zamora, Bernhard K. Mueller, Jessica C. Casciano, Jacob S. Brenner","doi":"10.1038/s44222-025-00314-5","DOIUrl":"10.1038/s44222-025-00314-5","url":null,"abstract":"The runaway success of the COVID-19 RNA–lipid nanoparticle (LNP) vaccine has set off a ‘gold rush’ to apply RNA–LNPs to other diseases, with an estimated value of US$50 billion and tremendous investment by industry and government in the last few years. Moving beyond vaccines, the natural focus for the field of nanomedicine has been cancer, for which most previous nanomedicines were developed. However, great opportunities lie outside cancer, especially among acute, deadly diseases such as stroke and heart attack, where the short-term expression of therapeutic mRNA fits well with the disease time course. Here, we review those opportunities, with a special focus on aspects of RNA–LNPs that present challenges for these non-malignant diseases. For example, the adjuvanticity of LNPs that helps in vaccines becomes unwanted inflammation when delivering therapeutics. In particular, we focus on safety issues that must not be ignored if RNA–LNPs are to avoid the deadly mistakes that risk setting the field back decades. By remaining clear-eyed about these challenges, we can use the multi-component, semi-modular nature of RNA–LNPs to create a new class of therapeutics that benefits dozens of diseases and injuries. Beyond vaccines, RNA–lipid nanoparticle (LNP) nanomedicines have originally focused on cancer applications. This Review discusses how these therapeutics can also benefit acute critical illnesses (ACIs) owing to the short-term expression of mRNA while mitigating the adjuvant effect of LNPs, which, unlike in vaccines and cancer, can be a liability for ACIs.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 9","pages":"775-790"},"PeriodicalIF":37.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyester-degrading enzymes in a circular economy of plastics 塑料循环经济中的聚酯降解酶

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-12 DOI: 10.1038/s44222-025-00308-3

Wolfgang Zimmermann

{"title":"Polyester-degrading enzymes in a circular economy of plastics","authors":"Wolfgang Zimmermann","doi":"10.1038/s44222-025-00308-3","DOIUrl":"10.1038/s44222-025-00308-3","url":null,"abstract":"With only a fraction of plastic waste being recovered and the majority incinerated, landfilled or released into the environment, plastic is increasingly accumulating in aquatic and terrestrial ecosystems, leading to widespread pollution. Biocatalysis offers a route to the sustainable recycling of synthetic polyesters, such as polyethylene terephthalate (PET), which is commonly used in textiles, food and beverage packaging, representing a major source of plastic waste. In particular, polyester hydrolases can deconstruct recalcitrant synthetic polymers at scale. This Review discusses the role of biocatalysis in a circular economy of plastics, outlining enzymatic modification and deconstruction strategies for synthetic polyesters. Moreover, protein engineering and computational approaches are examined to design and optimize polyester hydrolases for large-scale recycling in key industrial applications. Finally, the importance of economic viability is highlighted, including strategies to make biocatalysis a cost-effective and sustainable plastics recycling strategy. Plastic pollution could be partly addressed through the biocatalytic recycling of plastic waste streams. This Review discusses the identification and optimization of polyester-degrading enzymes for the large-scale recycling of plastic waste.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 8","pages":"681-696"},"PeriodicalIF":37.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interfacial T cell engineering 界面T细胞工程

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-12 DOI: 10.1038/s44222-025-00316-3

Yunus Alapan, Susan N. Thomas

{"title":"Interfacial T cell engineering","authors":"Yunus Alapan, Susan N. Thomas","doi":"10.1038/s44222-025-00316-3","DOIUrl":"10.1038/s44222-025-00316-3","url":null,"abstract":"Therapeutic T cell products and immunotherapies that target T cells require the manipulation of T cells ex vivo or in situ. Endogenous T cell functions are guided by their physical and biological interactions with other cells and tissues, occurring at the T cell membrane. Such T cell interfaces can be recapitulated using nanotechnologies and microtechnologies to precisely modulate and control T cell function. In this Review, we first discuss the structural and biochemical features of T cell interfaces at the cell and tissue levels, outlining how these interfaces dictate T cell function and fate. We then examine how T cell interfaces can be engineered at the nanoscale and microscale to create models for mechanistic studies, as well as scalable platforms for identifying, engineering, screening and sorting therapeutic T cells. Finally, we highlight key challenges and opportunities in the scaling and translation of these platforms for their integration into T cell therapy manufacturing processes. The function and fate of T cells are dictated by their various dynamic interactions with cells and tissues. This Review discusses the recreation of key T cell interfaces using nanotechnologies and microtechnologies for the mechanistic study of T cell biology, as well as the manufacturing and sorting of T cell products.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 7","pages":"549-564"},"PeriodicalIF":37.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal models in brain–computer interface research 脑机接口研究中的动物模型

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-06 DOI: 10.1038/s44222-025-00313-6

Yu Tung Lo, Lei Jiang, Jordan L. W. Lam, Ben Woodington, Elise P. W. Jenkins, Vasileios Christopoulos, Darrin J. Lee, Yuxin Liu, Charles Y. Liu

引用次数: 0

The power of public and patient involvement in healthcare innovation 公众和患者参与医疗保健创新的力量

IF 37.6

Nature reviews bioengineering Pub Date : 2025-05-01 DOI: 10.1038/s44222-025-00315-4

Vera Pinto Gomes, Matthew May, Jan Geissler, Steven Bourke

引用次数: 0