Nature reviews bioengineering最新文献_第3页

Sustainable production of chemicals by algorithm-assisted (bio)synthesis 通过算法辅助（生物）合成的可持续化学品生产

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-27 DOI: 10.1038/s44222-025-00312-7

Bartosz A. Grzybowski, Anna Żądło-Dobrowolska, Natalia Onishchenko, Eric S. Larsen

{"title":"Sustainable production of chemicals by algorithm-assisted (bio)synthesis","authors":"Bartosz A. Grzybowski, Anna Żądło-Dobrowolska, Natalia Onishchenko, Eric S. Larsen","doi":"10.1038/s44222-025-00312-7","DOIUrl":"10.1038/s44222-025-00312-7","url":null,"abstract":"Although research on computer-assisted planning of organic chemical syntheses dates back to the 1960s, it has only been in recent years that algorithms became powerful enough to autonomously design complete pathways, both in retrosynthetic and forward directions. Some of these modern programmes can plan chemically correct routes to complex targets and multiple such designs have now been validated by experiment. With chemical correctness secured, the next frontier for machines is to help design syntheses that are green (for example, in terms of conditions applied), sustainable (in terms of resources used) or circular (for example, in terms of waste feedstocks being revalorized). This Perspective argues that several parts of this challenge can benefit from close collaboration between synthetic chemists and bioengineers — for instance, to design better metrics of the environmental impact of syntheses or to develop algorithms with which to delineate the substrate scope of enzymatic transformations. Computers able to plan efficient and greener routes combining chemical and enzymatic steps will have a powerful and lasting impact on the production of fine chemicals. Synthesis-planning algorithms equipped with accurate knowledge of reaction rules can nowadays rival designs of expert organic chemists. This Review discusses how computer-assisted synthesis is used to help design routes that are not only chemically correct but also green, sustainable or circular.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 9","pages":"791-803"},"PeriodicalIF":37.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart closed-loop drug delivery systems 智能闭环给药系统

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-26 DOI: 10.1038/s44222-025-00328-z

Marco M. Paci, Tamoghna Saha, Omeed Djassemi, Steven Wu, Corrine Ying Xuan Chua, Joseph Wang, Alessandro Grattoni

{"title":"Smart closed-loop drug delivery systems","authors":"Marco M. Paci, Tamoghna Saha, Omeed Djassemi, Steven Wu, Corrine Ying Xuan Chua, Joseph Wang, Alessandro Grattoni","doi":"10.1038/s44222-025-00328-z","DOIUrl":"10.1038/s44222-025-00328-z","url":null,"abstract":"The administration of therapeutics for long-term chronic disease management or treatment faces considerable challenges, such as the need for precise dosage control, timely delivery and adherence to medication regimens. Traditional drug delivery methods often result in suboptimal therapeutic outcomes owing to variable responses, fluctuating drug concentrations and lack of feedback from real-time monitoring. Smart closed-loop systems (CLSs) could address these limitations by integrating real-time biosensing with automated drug delivery, thereby personalizing treatments to individual needs. This Review explores the current landscape of CLSs, highlighting recent advancements in wearable and implantable technologies that facilitate continuous monitoring of biomarkers and offer responsive therapeutic interventions. We discuss the implications of device design and the trade-offs between wearable and implantable systems. In addition, we highlight the potential of artificial intelligence enhancement of CLS control algorithms by enabling systems to learn from and predict responses to achieve more effective and adaptive optimal therapies. Ultimately, this Review charts a path towards next-generation CLSs, emphasizing the integration of synthetic biology and engineered cells into implantable devices. The administration of therapeutics for chronic disease management faces challenges like precise dosage control and timely delivery. This Review explores how smart closed-loop systems can address these issues by integrating real-time biosensing with automated drug delivery, highlighting advancements in wearable and implantable technologies, artificial intelligence-enhanced control algorithms and the integration of synthetic biology for personalized, adaptive therapies.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 10","pages":"816-834"},"PeriodicalIF":37.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145248859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome synthesis in plants 植物基因组合成

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-25 DOI: 10.1038/s44222-025-00326-1

Tianlong Lan (, ), Lian-Ge Chen (, ), Ying Wang (, ), Yuling Jiao (, )

{"title":"Genome synthesis in plants","authors":"Tianlong Lan \u0000 (, ), Lian-Ge Chen \u0000 (, ), Ying Wang \u0000 (, ), Yuling Jiao \u0000 (, )","doi":"10.1038/s44222-025-00326-1","DOIUrl":"10.1038/s44222-025-00326-1","url":null,"abstract":"Owing to advances in genome sequencing and editing, a genome can now be redesigned, synthesized and introduced into cells as desired. The field of synthetic genomics not only aims to provide deeper understanding of how the genome functions but can also be harnessed for a wide range of synthetic biology and bioengineering applications, from rapid evolution and screening for favourable strains to biotechnological and bioproduction tool development. Although genome synthesis has been carried out mainly in simple unicellular organisms, plants and animals are now also being investigated. Compared with animals, plants have unique advantages, such as fewer ethical concerns, simpler experimental operations and easier regeneration from cells to organisms. In this Review, we focus on genome synthesis in plants, discuss the current research landscape and assess possible future directions. Owing to advances in genome sequencing and editing, a genome can now be redesigned, synthesized and introduced into cells as desired. This Review discusses plant genome synthesis, highlighting bottom-up genome design, large-fragment assembly and site-directed targeting.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 10","pages":"875-889"},"PeriodicalIF":37.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145248857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly insect control in crop production 作物生产中的生态友好型昆虫控制

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-18 DOI: 10.1038/s44222-025-00332-3

Eva M. Steiner-Rebrova, Kenneth V. Halberg

引用次数: 0

Writing is thinking 写作就是思考

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-16 DOI: 10.1038/s44222-025-00323-4

引用次数: 0

Ex vivo organ engineering using decellularized tissue scaffolds 使用脱细胞组织支架的离体器官工程

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-16 DOI: 10.1038/s44222-025-00322-5

Tarek Saleh, Lorenzo Caciolli, Giovanni Giuseppe Giobbe, Paolo De Coppi

{"title":"Ex vivo organ engineering using decellularized tissue scaffolds","authors":"Tarek Saleh, Lorenzo Caciolli, Giovanni Giuseppe Giobbe, Paolo De Coppi","doi":"10.1038/s44222-025-00322-5","DOIUrl":"10.1038/s44222-025-00322-5","url":null,"abstract":"End-stage organ failure requires the transplantation of a new organ. However, the number of patients awaiting donor organs exceeds the number of available organs. To address this organ shortage, solid organs, such as the kidneys, liver, heart and lungs, can be engineered based on decellularized human and non-human tissues. These decellularized scaffolds can then be recellularized with autologous or allogeneic cells and modified to ensure engraftment and function following transplantation. In this Review, we discuss the creation of decellularized neo-solid organs, including animal donor considerations, pre-decellularization processes, decellularization protocols, post-decellularization characterization, sterilization and storage conditions. We highlight various cell seeding and modification strategies and examine bioreactor culture conditions to grow functional solid organs. Finally, we outline mechanisms of transplant-recipient crosstalk and discuss challenges and opportunities for the clinical translation of engineered solid organs. New organs can be engineered based on decellularized human and animal tissues to address the global shortage of donor organs. This Review discusses design principles for the engineering of new organs using decellularized animal-derived tissues, including decellularization, functionalization and characterization protocols.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 9","pages":"761-774"},"PeriodicalIF":37.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zwitterionic polymers as PEG replacements for improved mRNA delivery 两性离子聚合物作为改善mRNA传递的PEG替代品

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-16 DOI: 10.1038/s44222-025-00333-2

Christine-Maria Horejs

引用次数: 0

Polyethylene glycol immunogenicity in nanomedicine 聚乙二醇在纳米医学中的免疫原性

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-10 DOI: 10.1038/s44222-025-00321-6

Yunching Chen (, ), Yu-Cheng Su (, ), Steve R. Roffler

{"title":"Polyethylene glycol immunogenicity in nanomedicine","authors":"Yunching Chen \u0000 (, ), Yu-Cheng Su \u0000 (, ), Steve R. Roffler","doi":"10.1038/s44222-025-00321-6","DOIUrl":"10.1038/s44222-025-00321-6","url":null,"abstract":"Polyethylene glycol (PEG) endows nanomedicines with stealth properties, reducing interactions with immune cells, prolonging blood circulation and stabilizing lipid-based formulations. However, anti-PEG antibodies, either pre-existing or induced by PEGylated medicines and vaccines, might adversely affect the safety and efficacy of nanomedicines by altering nanocarrier biodistribution, inducing unwanted inflammatory responses, destabilizing lipid formulations and causing hypersensitivity reactions. Therefore, the effect of PEG immunogenicity on nanomedicines should be critically assessed, and alternative approaches explored. In this Review, we first discuss PEG immunogenicity and the sources, detection and effects of anti-PEG antibodies. We then highlight strategies to address PEG immunogenicity, including the adjustment of dosing, routes and timing of nanomedicine administration, competition with high-molecular-mass PEG, engineering strategies to improve stealth effects of PEG and the design of complement inhibitors to reduce opsonization. In addition, we examine approaches for the design of PEG-free stealth nanomedicines, such as the use of alternative polymers, protein nanocages and biomimetic particles cloaked with cell membranes, serum components or bioactive molecules to prevent immune system recognition. Finally, we explore the application of anti-PEG antibodies in the creation of artificial cell receptors, reloadable hydrogels and bispecific antibodies for targeted delivery of PEGylated therapeutics. Polyethylene glycol (PEG) is used in many nanomedicines but might cause the production of anti-PEG antibodies. This Review critically assesses mechanisms and effects of PEG immunogenicity and explores strategies to address PEG immunogenicity in the context of nanomedicine.","PeriodicalId":74248,"journal":{"name":"Nature reviews bioengineering","volume":"3 9","pages":"742-760"},"PeriodicalIF":37.6,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145122918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving transfusion medicine in resource-limited settings by point-of-care diagnostics 通过即时诊断改善资源有限地区的输血医学

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-10 DOI: 10.1038/s44222-025-00331-4

Rijo Rajeev, Paresh Mohanty, Suvro Sankha Datta, Parikshit Moitra

引用次数: 0

Engineering cell-based ovarian hormone therapy 工程细胞卵巢激素治疗

IF 37.6

Nature reviews bioengineering Pub Date : 2025-06-05 DOI: 10.1038/s44222-025-00325-2

Maria João Sousa, Christiani A. Amorim

引用次数: 0