Engineered regeneration最新文献_第8页

One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes 基于仿生光子条形码的一步免清洗多重免疫分析平台

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.03.007

Dagan Zhang , Yuze Wang , Junqi Zhao , Xueqin Li , Yuanyang Zhou , Sen Wang

{"title":"One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes","authors":"Dagan Zhang , Yuze Wang , Junqi Zhao , Xueqin Li , Yuanyang Zhou , Sen Wang","doi":"10.1016/j.engreg.2023.03.007","DOIUrl":"10.1016/j.engreg.2023.03.007","url":null,"abstract":"<div><p>Multiplex, rapid and accurate virus quantification plays a great value in biomedical detection. Here, a novel one step, wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was explored. PhC barcodes were decorated with PDA by self-polymerization of DA, thus this nanocomposite hybridized PhC barcodes facilitated the adsorption of FITC labelled antibodies and quenched itself photoluminescent, allowing a fast responsive composite platform. In the presence of target analyte, the FITC-labelled detection antibody was released from the surface of PDA decorated microcarrier to specifically bind to the target analyte, thus recovered the photoluminescence. In addition, the PhC microcarrier was enabled to carry out various color barcode for different targets detection though tuning internal periodic structures. Based on these excellent performances of the nanocomposite barcode, this method can not only capture H1N1, H5N1, SARS-CoV-2 simultaneously with rapid, accuracy but also accomplish multiplex quantification detection with high-sensitivity. Furthermore, our developed platform was also achieved with high-sensitivity and high-specificity through the verification of clinical samples, thus laying out a new avenue for multiplex virus detection in clinical diagnosis.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 238-244"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46911267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Developing hierarchical microneedles for biomedical applications 开发用于生物医学应用的分级微针

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.04.004

Minhui Lu , Xiaoxuan Zhang , Zhiqiang Luo , Yuanjin Zhao

{"title":"Developing hierarchical microneedles for biomedical applications","authors":"Minhui Lu , Xiaoxuan Zhang , Zhiqiang Luo , Yuanjin Zhao","doi":"10.1016/j.engreg.2023.04.004","DOIUrl":"10.1016/j.engreg.2023.04.004","url":null,"abstract":"<div><p>As a new kind of microcarrier device, microneedles are featured by micrometer needle arrays with an overall size in the centimeter scale. Due to the needle shape and the micron size, microneedles can penetrate the skin without harming nerves and blood vessels, which causes many advantages such as minimally invasive, safe and convenient. The past few decades have witnessed a great leap in microneedles research. The main materials of microneedles have changed from metal and ceramic to polymers with more complex functions, and the optimization of materials and preparation strategies has led to a greater variety of microneedle styles. Among them, the construction or combination of smaller size structures or materials on microneedles to fabricate hierarchical microneedles is a major research hotspot. Here, we present the recent research progress of hierarchical microneedles for biomedicine. We begin by discussing the fabrication strategies of hierarchical microneedles, including mainstream casting and coating methods based on microneedle molds and three dimensions (3D) printing methods. We then expand the discussion from the hierarchical microneedles with porous structure to those composited with nanomaterials. Eventually, we have a discussion about the research progress of hierarchical microneedles in the area of biomarkers detection and transdermal drug delivery, as well as its future development direction.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 316-327"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45465444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy mediates osteoporotic bone regeneration induced by micro-/nano-structured modification on hydroxyapatite bioceramics 自噬介导羟基磷灰石生物陶瓷微/纳米结构修饰诱导的骨质疏松性骨再生

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.03.003

Jinjie Cui , Xinran Zhang , Liming Cheng , Kaili Lin

{"title":"Autophagy mediates osteoporotic bone regeneration induced by micro-/nano-structured modification on hydroxyapatite bioceramics","authors":"Jinjie Cui , Xinran Zhang , Liming Cheng , Kaili Lin","doi":"10.1016/j.engreg.2023.03.003","DOIUrl":"10.1016/j.engreg.2023.03.003","url":null,"abstract":"<div><p>Osteoporosis (OP) is an age-related disease of bone metabolism, characterized by bone mass loss and bone microarchitecture deterioration, the poor osteogenesis microenvironment of OP caused hardly repairing of the bone defects. As a dynamic process to fuel cellular renovation, autophagy has been proved to play a vital role in regulating cell differentiation and maintaining bone homeostasis. Traditional bone repairing biomaterials are hardly repairing the bone defects under OP pathological microenvironment. Therefore, it is essential to development novel biomaterials to improve osteoporotic osteogenesis. Compared to biochemical cues, biophysical cues exhibited more advantages in biocompatible and side effects. Herein, inspired by the importance of enhanced autophagic response in osteoporotic environment, we intend to utilize the micro-/nano-structured hydroxyapatite (mnHA) bioceramics as the mimic structure of natural bone tissue to regulate autophagic activity in ovariectomy bone mesenchymal stem cells (OVX-BMSCs), finally promote to bone regeneration in OP condition. The results indicated that mnHA bioceramics promoted cell adhesion and osteogenesis of OVX-BMSCs, and enhanced autophagy level in OVX-BMSCs. In the calvarial defects of OVX-rats, the mnHA scaffold acquired excellent bone repair effect. According to the current findings, regulating the level of autophagy could be a promising strategy for improve osteoporotic osteogenesis in the future.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 245-256"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43857057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Accelerated neutral atom beam (ANAB) and gas clustered ion beam (GCIB) treatment of implantable device polymers leads to decreased bacterial attachment in vitro and decreased inflammation in vivo 可植入装置聚合物的加速中性原子束（ANAB）和气体簇离子束（GCIB）治疗可减少体外细菌附着和体内炎症

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.03.006

Joseph Khoury , Ti Zhang , David B. Earle , M. Laird Forrest

引用次数: 0

Engineering of electrospun nanofiber scaffolds for repairing brain injury 电纺纳米纤维支架修复脑损伤的工程化研究

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.04.001

Wenzhe Du , Tong Wang , Shuyun Hu , Jixin Luan , Feng Tian , Guolin Ma , Jiajia Xue

{"title":"Engineering of electrospun nanofiber scaffolds for repairing brain injury","authors":"Wenzhe Du , Tong Wang , Shuyun Hu , Jixin Luan , Feng Tian , Guolin Ma , Jiajia Xue","doi":"10.1016/j.engreg.2023.04.001","DOIUrl":"10.1016/j.engreg.2023.04.001","url":null,"abstract":"<div><p>Patients with brain injury can suffer disability and accompanying complications. Current clinical treatments have significant limitations to successful repair due to the complexity of the pathological processes and the inhibitory microenvironment that follows brain injury. Here, we conclude recent research progresses in engineering strategies based on electrospun nanofibers for promoting neural repair and functional recovery after brain injury. Firstly, we introduce the main pathological mechanisms of current brain injuries, pointing out the prospect of the application of electrospun nanofiber scaffolds compared to current clinical treatment strategies. We then discuss the repair strategies combining the structure and the morphology of nanofiber scaffolds with load therapeutic factors such as cells, drugs and growth factors. All of these strategies show potential for improving the repair of brain injury. Finally, we point out the challenges facing the effective treatment of brain injury, aiming to provide insights into the development of repairing scaffolds for brain function recovery from the perspective of clinical treatment.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 289-303"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42286451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel metal nanomaterials to promote angiogenesis in tissue regeneration 新型金属纳米材料促进组织再生血管生成

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.03.008

Yuki G. Yoshida , Su Yan , Hui Xu , Jian Yang

引用次数: 5

Heparinized PGA host-guest hydrogel loaded with paracrine products from electrically stimulated adipose-derived mesenchymal stem cells for enhanced wound repair 肝素化PGA主客体水凝胶装载电刺激脂肪源间充质干细胞的旁分泌产物，用于增强伤口修复

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.03.002

Mengyao Li , Junliang Li , Xueru Xiong , Yushi Wang , Yong-Guang Jia , Xuetao Shi , Xiaoling Fu

{"title":"Heparinized PGA host-guest hydrogel loaded with paracrine products from electrically stimulated adipose-derived mesenchymal stem cells for enhanced wound repair","authors":"Mengyao Li , Junliang Li , Xueru Xiong , Yushi Wang , Yong-Guang Jia , Xuetao Shi , Xiaoling Fu","doi":"10.1016/j.engreg.2023.03.002","DOIUrl":"10.1016/j.engreg.2023.03.002","url":null,"abstract":"<div><p>The microenvironment of the wound bed is essential in the regulation of wound repair. In this regard, strategies that provide a repairing favorable microenvironment may effectively improve healing outcomes. Herein, we attempted to use electrical stimulation (ES) to boost the paracrine function of adipose-derived stem cells from rats (rASCs). By examining the concentrations of two important growth factors, VEGF and PDGF-AA, in the cell culture supernatant, we found that ES, especially 5 μA ES, stimulated rASCs to produce more paracrine factors (5 μA-PFs). Further studies showed that ES may modulate the paracrine properties of rASCs by upregulating the levels of TRPV2 and TRPV3, thereby inducing intracellular Ca<sup>2+</sup> influx. To deliver the PFs to the wound to effectively improve the wound microenvironment, we prepared a heparinized PGA host-guest hydrogel (PGA-Hp hydrogel). Moreover, PGA-Hp hydrogel loaded with 5 μA-PFs effectively accelerated the repair process of the full-thickness wound model in rats. Our findings revealed the effects of ES on the paracrine properties of rASCs and highlighted the potential application of heparinized PGA host-guest hydrogels loaded with PFs derived from electrically stimulated rASCs in wound repair.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 225-237"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46556465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The overexpression of Rps14 in Lgr5+ progenitor cells promotes hair cell regeneration in the postnatal mouse cochlea Rps14在Lgr5+祖细胞中的过表达促进了出生后小鼠耳蜗毛细胞的再生

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.04.006

Jingru Ai , Pei Jiang , Jingyuan Huang , Hairong Xiao , Yanqin Lin , Mingchen Dai , Yintao Wang , Yuan Zhang , Mengyao Tian , Renjie Chai , Shasha Zhang

{"title":"The overexpression of Rps14 in Lgr5+ progenitor cells promotes hair cell regeneration in the postnatal mouse cochlea","authors":"Jingru Ai , Pei Jiang , Jingyuan Huang , Hairong Xiao , Yanqin Lin , Mingchen Dai , Yintao Wang , Yuan Zhang , Mengyao Tian , Renjie Chai , Shasha Zhang","doi":"10.1016/j.engreg.2023.04.006","DOIUrl":"10.1016/j.engreg.2023.04.006","url":null,"abstract":"<div><p>Sensory hair cells (HCs) in the cochlea cannot regenerate spontaneously in adult mammals after being damaged by external or genetic factors. However, several genes and signaling pathways are reported to induce cochlear HC regeneration at the early neonatal stage. <em>Rps14</em> encodes a ribosomal protein that is involved in the regulation of cell differentiation and proliferation in mammals. However, its roles in the cochlea have not been reported <em>in vivo</em>. Here, we specifically overexpressed <em>Rps14</em> in Lgr5+ progenitor cells in the newborn mouse cochlea and found that <em>Rps14</em> conditional overexpression (cOE) mice had significantly increased the ectopic HCs, including inner and outer HCs. We further explored the source of these ectopic HCs and found no EdU<em>+</em> supporting cells observed in the <em>Rps14</em> cOE mice. The lineage tracing results, on the other hand, revealed that <em>Rps14</em> cOE mice had significantly more tdTomato<em>+</em> HCs in their cochleae than control mice. These results indicated that regenerated HCs by cOE of <em>Rps14</em> are most likely derived from inducing the direct trans-differentiation of Lgr5+ progenitor cells into HCs. Moreover, real-time qPCR results suggested that the transcription factor genes <em>Atoh1</em> and <em>Gfi1</em>, which are important in regulating HC differentiation, were upregulated in the cochlear basilar membrane of <em>Rps14</em> cOE mice. In summary, this study provides <em>in vivo</em> evidence that, in the postnatal mouse cochlea, <em>Rps14</em> is a potential gene that can promote the spontaneous trans-differentiation of Lgr5+ progenitor cells into HCs. This gene may one day be exploited as a therapeutic target for treating hearing loss.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 328-336"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42927308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Driving modes and characteristics of biomedical micro-robots 生物医学微型机器人驱动模式及特性研究

Engineered regeneration Pub Date : 2023-08-09 DOI: 10.1016/j.engreg.2023.08.001

Libing Huang , Yueyuan Pan , Miao Wang , Lei Ren

{"title":"Driving modes and characteristics of biomedical micro-robots","authors":"Libing Huang , Yueyuan Pan , Miao Wang , Lei Ren","doi":"10.1016/j.engreg.2023.08.001","DOIUrl":"10.1016/j.engreg.2023.08.001","url":null,"abstract":"<div><p>Micro-robots (MRs) are miniature machines with dimensions smaller than 1 mm and have semi- or fully-autonomous capabilities, including sensing, decision-making, and performing operations. These MRs have garnered significant attention in the precision medicine and personalized treatment field due to their ability to navigate narrow areas of the human body with non-desirable fluid flow. Specifically, MRs are actuated by a mechanism that generates propulsive force through the interaction between MRs' actuation modules and external energy sources in a specific direction. This driving mechanism enables the precise execution of medical treatment such as targeted drug delivery and minimally invasive surgeries. Nonetheless, MRs currently encounter certain challenges in clinical practice, including reliance on external energy sources, short lifespan, and difficulties in degradation or recovery within the human body. This article aims to review the common components and characteristics of driving mechanism for MRs' actuation modules, propose possible solutions to address current clinical challenges, and ultimately, explore the desirable structural and functional composition for the future development of MRs. Through these efforts, this review hopes to provide guidance for the future development of MRs in the field of precision medicine.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 4","pages":"Pages 411-426"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44807402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persuasive factors on the bioink printability and cell viability in the extrusion-based 3D bioprinting for tissue regeneration applications 组织再生应用中基于挤出的3D生物打印中生物墨水可打印性和细胞活力的说服因素

Engineered regeneration Pub Date : 2023-07-29 DOI: 10.1016/j.engreg.2023.07.002

Devara Venkata Krishna, Mamilla Ravi Sankar

{"title":"Persuasive factors on the bioink printability and cell viability in the extrusion-based 3D bioprinting for tissue regeneration applications","authors":"Devara Venkata Krishna, Mamilla Ravi Sankar","doi":"10.1016/j.engreg.2023.07.002","DOIUrl":"10.1016/j.engreg.2023.07.002","url":null,"abstract":"<div><p>The extrusion-based bioprinting (EBBP) applications in the medical field tremendously increase due to its versatility in fabricating intricate geometry components with reasonable accuracy and precision. The bioink and its properties for an EBBP process are crucial in manufacturing parts with significant biocompatibility and functionality. The EBBP demands optimized parameters for obtaining good printability and cell viability. A better understanding of the various process parameters is essential for the researcher to optimize the mechanical and biological properties of the printed constructs. The biological, mechanical, and rheological parameters all together need to be evaluated to enhance the printability of tissue. This article concisely delineates the effect of the rheological and physiochemical parameters on the biological and mechanical properties of the printed tissues. The printing parameters and nozzle geometry, which considerably influence the printability, and shape fidelity of the bioprinted scaffolds are exemplified in detail. Additionally, the challenges and future aspects of enhancing printability are discussed succinctly.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 4","pages":"Pages 396-410"},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44577047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1