{"title":"Emerging microfluidic technologies for sperm sorting","authors":"Junjie Huang, Hanxu Chen, Ning Li, Yuanjin Zhao","doi":"10.1016/j.engreg.2023.02.001","DOIUrl":"10.1016/j.engreg.2023.02.001","url":null,"abstract":"<div><p>Sorting high-quality sperm with intact DNA, normal morphology, and active motility is crucial for clinically assisted reproductive technology, which influences the success of treatment and the health of offspring. Currently, microfluidic technology has been developed as a powerful platform for sperm sorting owing to its ability to manipulate fluid at the microscale and handle small samples. Specifically, microfluidic technology provides the necessary stimuli including fluid stimulus, chemical induction, and shape sift, which supports researchers in developing various sperm-sorting devices. According to the sorting principle, these devices can be divided into three categories: active sorting devices based on sperm rheological properties, passive sorting devices based on sperm physical properties, and external stimuli-induced sorting devices. Hence, we review a broad range of researches about sperm sorting with microfluidics and briefly present the properties of sperm and female reproductive tract to assist the design of microfluidic sperm sorting devices.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 161-169"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49027473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yingchao Su , Matthew Cappock , Stephanie Dobres , Allan J. Kucine , Wayne C. Waltzer , Donghui Zhu
{"title":"Supplemental mineral ions for bone regeneration and osteoporosis treatment","authors":"Yingchao Su , Matthew Cappock , Stephanie Dobres , Allan J. Kucine , Wayne C. Waltzer , Donghui Zhu","doi":"10.1016/j.engreg.2023.02.003","DOIUrl":"10.1016/j.engreg.2023.02.003","url":null,"abstract":"<div><p>Mineral ions play a crucial role in various biological processes in the human body, particularly in bone repair and regeneration. Supplementation with mineral ions offers several advantages over other therapies or treatments for bone repair and regeneration, such as higher biosafety, universal applicability, and compatibility with the immune system. Additionally, supplementation with mineral ions may avoid the need for invasive surgical procedures. The aim of this review is to provide a comprehensive overview of the functions of potentially beneficial mineral ions and their effects on bone regeneration and osteoporosis treatment. By examining previous studies, including in vitro cellular experiments, in vivo animal models, and clinical trials, this review compares the benefits and potential adverse effects of these mineral ions. Moreover, the review provides guidelines for suggested daily supplementation of these mineral ions to assist future preclinical and clinical studies in bone regeneration and osteoporosis treatment.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 170-182"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49259080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yangnan Hu , Han Zhang , Hao Wei , Menghui Liao , Xiaoyan Chen , Jiayue Xing , Lian Duan , Cuntu Cheng , Weicheng Lu , Xuechun Yang , Peina Wu , Huan Wang , Jingdun Xie , Renjie Chai
{"title":"Conductive PS inverse opals for regulating proliferation and differentiation of neural stem cells","authors":"Yangnan Hu , Han Zhang , Hao Wei , Menghui Liao , Xiaoyan Chen , Jiayue Xing , Lian Duan , Cuntu Cheng , Weicheng Lu , Xuechun Yang , Peina Wu , Huan Wang , Jingdun Xie , Renjie Chai","doi":"10.1016/j.engreg.2023.03.001","DOIUrl":"10.1016/j.engreg.2023.03.001","url":null,"abstract":"<div><p>The development of neural tissue engineering has brought new hope to the treatment of spinal cord injury (SCI). Up to date, various scaffolds have been developed to induce the oriented growth and arrangement of nerves to facilitate the repair after injury. In this work, a conductive and anisotropic inverse opal substrate was presented by modifying polystyrene (PS) inverse opal films with carbon nanotubes and then stretching them to varying degrees. The film had good biocompatibility, and neural stem cells (NSCs) grown on the film displayed good orientation along the stretching direction. In addition, benefiting from the conductivity and anisotropy of the film, NSCs differentiated into neurons significantly. These results suggest that the conductive and anisotropic PS inverse opal substrates possess value in nerve tissue engineering regeneration.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 214-221"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41364665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structure and mechanics of native and decellularized porcine cranial dura mater","authors":"Ashma Sharma , Jun Liao , Lakiesha N. Williams","doi":"10.1016/j.engreg.2023.02.004","DOIUrl":"10.1016/j.engreg.2023.02.004","url":null,"abstract":"<div><p>The dura mater is the outermost layer of meninges and consists of a dense elastic membrane that keeps cerebrospinal fluid inside the cavity. In most cranial surgical interventions, the dura mater is incised and needs to be repaired with a graft replacement. We assessed decellularized porcine dura mater as a novel graft material by quantifying the mechanical and structural properties of the dura membrane. Porcine dura mater was decellularized using the Sodium Dodecyl Sulfate (SDS) technique and subjected to uniaxial tensile testing, micro indentation testing, histological analysis, and Transmission Electron Microscopy (TEM). For native dura, we found the tensile modulus in the linear region (15%-25% strain) to be 19.31 ± 1.23 MPa, with an initial tensile modulus (0%-3.5% strain range) of 451 ± 0.30 kPa, and the failure stress as 4.61 ± 1.50 MPa at 35% strain. For decellularized dura, the tensile modulus in the linear region was 10.81 ± 0.88 MPa, the initial tensile modulus was 226 ± 22 kPa, and the failure stress was 4.55 ± 1.05 MPa at 55% strain. The effective compressive modulus was 7 to 19 kPa and 19–57 kPa for the native dura and the decellularized dura, respectively. Our histological and TEM observations showed that the orientation of fibers within the dura was maintained after decellularization. In short, our study demonstrated that decellularized porcine dura was able to maintain its overall morphological/structural integrity and preserve the native dura's mechanical behavior, which provides a solid foundation for its use as a functional grafting material.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 205-213"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43155768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
James Chung-Wai Cheung , Daphne Sze Ki Cheung , Ming Ni , Kam-Wai Chen , Ye-Jiao Mao , Lin Feng , Wing-Kai Lam , Duo Wai-Chi Wong , Aaron Kam-Lun Leung
{"title":"X-reality for phantom limb management for amputees: A systematic review and meta-analysis","authors":"James Chung-Wai Cheung , Daphne Sze Ki Cheung , Ming Ni , Kam-Wai Chen , Ye-Jiao Mao , Lin Feng , Wing-Kai Lam , Duo Wai-Chi Wong , Aaron Kam-Lun Leung","doi":"10.1016/j.engreg.2023.02.002","DOIUrl":"10.1016/j.engreg.2023.02.002","url":null,"abstract":"<div><p>Phantom limb is a disabling neuropsychiatric condition among amputees resulting in pain and disturbance that impact their functions, quality of life, and autonomy. While pharmacological approaches appeared to be ineffective, the emergence and integration of X-reality, including virtual reality, augmented reality, and mixed reality, might elevate the effectiveness of mirror therapy in managing phantom limb. The objective of this study is to review X-reality for managing phantom pain. A systematic search was conducted on PubMed, Scopus, Web of Science, PsycINFO, Embase, and CINAHL. Sixteen (<em>n</em> = 16) studies containing 66 lower-limb and 53 upper-limb amputees were included for the review over the thematic framework of amputee characteristics and intervention designs, while thirteen (<em>n</em> = 13) studies were further proceeded for the meta-analysis. We found eleven studies on virtual reality (<em>n</em> = 11), four studies on marker-based augmented reality (<em>n</em> = 4) and one study on mixed reality (<em>n</em> = 1) with a total of 40 game/task themes involving, motor skills, motor control, and stimulus-sensing. Regardless, all these interventions adopted the movement representation strategies with different techniques. Overall, the X-reality interventions reduced the pain level of the amputees (mean difference: -2.30, 95% CI, -3.38 to -1.22), especially the virtual reality subgroup (mean difference: -2.83, 95% CI, -4.43 to -1.22). However, there were substantial heterogeneity and partially explained by the subgroup analysis on publication year. The strength of evidence was limited by case reports and case series in this review.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 134-151"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48156559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mengkun Fang , Han Zhang , Yuze Wang , Hui Zhang , Dagan Zhang , Peipei Xu
{"title":"Biomimetic selenium nanosystems for infectious wound healing","authors":"Mengkun Fang , Han Zhang , Yuze Wang , Hui Zhang , Dagan Zhang , Peipei Xu","doi":"10.1016/j.engreg.2023.01.004","DOIUrl":"10.1016/j.engreg.2023.01.004","url":null,"abstract":"<div><p>Bacteria-related wound infection and healing have been a major issue for patients and health-care systems for decades. The rise and evolution of effective treatment will result in significant benefits to human beings. In addition to standard antibacterial drugs, a combination of nanoparticles (NPs) and biological membranes is widely applied as a novel antibacterial agent against infectious pathogens. In this paper, the red blood cell membrane-encapsulated selenium nanoparticles (R-SeNPs) were fabricated for infectious wound healing. The stability, the immune evading capability, and the internal circulation time of the R-SeNPs were notably enhanced compared with those of bare selenium nanoparticles (SeNPs). Moreover, <em>in vivo</em> studies demonstrated the outstanding performance of the R-SeNPs in infectious wound healing. The biomimetic selenium nanosystem demonstrated the benefits of the combination of nanotechnology and bionics design and will contribute to wound healing in the future.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 152-160"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48265562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaojun Hu , Ruoyan Wei , Chang Liu , Yunzhe Wang , Danjuan Yang , Ling Sun , Fei Xia , Shengtao Liu , Meiyan Li , Xingtao Zhou
{"title":"Recent advances in small incision lenticule extraction (SMILE)-derived refractive lenticule preservation and clinical reuse","authors":"Xiaojun Hu , Ruoyan Wei , Chang Liu , Yunzhe Wang , Danjuan Yang , Ling Sun , Fei Xia , Shengtao Liu , Meiyan Li , Xingtao Zhou","doi":"10.1016/j.engreg.2023.01.002","DOIUrl":"10.1016/j.engreg.2023.01.002","url":null,"abstract":"<div><p>Small incision lenticule extraction (SMILE) has become one of the mainstream refractive surgeries in recent years, with satisfactory efficacy, safety, and predictability. SMILE-derived refractive lenticule, the byproduct of the surgery, holds great potential in clinical practice given its easy access and good biocompatibility. Numerous studies have been published to describe its applications in refractive correction, corneal ectasia diseases, and corneal defects. The feasibility and safety were validated in both animal models and clinical studies. Moreover, the preservation method is also crucial for its further promotion and application. Novel techniques are also evaluated and applied in lenticule preservation. We covered the recent advances in the preservation of corneal stromal lenticules and their clinical reuse in this review.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 103-121"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44144187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Derek Ka-Hei Lai , Li-Wen Zha , Tommy Yau-Nam Leung , Andy Yiu-Chau Tam , Bryan Pak-Hei So , Hyo Jung Lim , Daphne Sze Ki Cheung , Duo Wai-Chi Wong , James Chung-Wai Cheung
{"title":"Corrigendum to ‘Dual ultra-wideband (UWB) radar-based sleep posture recognition system: Towards ubiquitous sleep monitoring’[Engineered Regeneration 4 (2023) 36 – 43]","authors":"Derek Ka-Hei Lai , Li-Wen Zha , Tommy Yau-Nam Leung , Andy Yiu-Chau Tam , Bryan Pak-Hei So , Hyo Jung Lim , Daphne Sze Ki Cheung , Duo Wai-Chi Wong , James Chung-Wai Cheung","doi":"10.1016/j.engreg.2023.03.005","DOIUrl":"10.1016/j.engreg.2023.03.005","url":null,"abstract":"","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Page 223"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43091198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Natural Biopolymers for Bone Tissue Engineering: A Brief Review","authors":"Sheersha Pramanik , Shubham Kharche , Namdev More , Deepak Ranglani , Gajendra Singh , Govinda Kapusetti","doi":"10.1016/j.engreg.2022.12.002","DOIUrl":"10.1016/j.engreg.2022.12.002","url":null,"abstract":"<div><p>Tissue engineering is a well-proven technique for the creation of functional alternatives for regenerative medicine and plays a critical role in patient treatment. Several natural-origin biopolymers such as chitosan, hyaluronic acid, gelatin, collagen, etc. are extensively explored for various biomedical applications. Among, these polymers are exclusively investigated in tissue engineering applications due to their highly favorable properties, such as high biocompatibility, slow degradation, mechanical tenability, structural similarity with native tissues, bioactivity, etc. The present review summarizes the recent advances of biopolymers in bone tissue engineering It also covers the topic of natural polymer modification to achieve superior characteristics primarily mechanical properties towards bone regeneration and discussed the best methods for dealing with them. Therefore, the review can drive the development of biomimetic materials for futuristic applications.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 2","pages":"Pages 193-204"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42558768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}