Engineered regeneration最新文献

{"title":"Polylysine complexes and their biomedical applications","authors":"Haofang Zhu,&nbsp;Rui Liu,&nbsp;Yixuan Shang,&nbsp;Lingyun Sun","doi":"10.1016/j.engreg.2022.11.001","DOIUrl":"10.1016/j.engreg.2022.11.001","url":null,"abstract":"<div><p>Poly-L-lysine (PLL) is a water-soluble biopolymer consist of repeating unit of L-lysine. It displays intrinsic non-antigenicity, antibacterial property, biocompatibility, and biodegradability, and facile synthesis procedure, therefore has attracted particular attention in various biomedical and pharmaceutical applications. Following a brief introduction to PLL synthesis, this review focuses on the significant advances of PLL and PLL-derived complexes utilized in cargo delivery, tissue adhesives, and antibacterial materials. Meanwhile, this review summarized the modification and optimization of PLL complexes for further clinical translational studies. The final section discusses the challenges and opportunities of PLL-based complexes in the biomedical areas.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 1","pages":"Pages 20-27"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47001256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced surgical technologies for lung cancer treatment: Current status and perspectives","authors":"Hongfei Cai ,&nbsp;Yonghui Wang ,&nbsp;Da Qin ,&nbsp;Youbin Cui ,&nbsp;Hongbo Zhang","doi":"10.1016/j.engreg.2022.12.001","DOIUrl":"10.1016/j.engreg.2022.12.001","url":null,"abstract":"<div><p>Lung cancer has become one of the key life-threatening factors and is the most common type of tumor. With the improvement of equipment and the improvement of lung cancer awareness, the surgical treatment of lung cancer is becoming more and more mature. Surgery can provide an important part of individualized treatment strategies for patients with different stages of lung cancer and different wishes. In addition, the concept of minimally invasive, precise, and intelligent has triggered a revolutionary change in the surgical treatment strategy for lung cancer. Therefore, this review focuses on the development of lung cancer surgery history, summarizing the era from traditional surgery to the era of minimally invasive thoracic surgery based on TV-assisted thoracic surgery. The operating methods and treatment effects of different surgical methods are comprehensively introduced, and the optimization and improvement of different surgical methods in the future are also discussed. Along with the concept of minimally invasive surgical techniques, there are more and more explorations of nanotechnology in the surgical treatment of lung cancer. The application of nanotechnology in lung cancer imaging, and the combination of surgery with nanomedicine is an effective solution for cancer treatment today. So, this review also summarizes the application prospects of nanotechnology before, during and after lung cancer surgery.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 1","pages":"Pages 55-67"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44340835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of modified PMMA cement in spine surgery","authors":"Zhikun Li ,&nbsp;Junwei Shi ,&nbsp;Yi Wang ,&nbsp;Yifan Li ,&nbsp;Wenjun Liu ,&nbsp;Ruijun Xu ,&nbsp;Silian Wang ,&nbsp;Liwei Chen ,&nbsp;Xiaojian Ye ,&nbsp;Chi Zhang ,&nbsp;Wei Xu","doi":"10.1016/j.engreg.2023.06.001","DOIUrl":"https://doi.org/10.1016/j.engreg.2023.06.001","url":null,"abstract":"","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49905036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Strategies of Islet Product for Endocrine Regeneration","authors":"Yue Cheng, Gang Zhao","doi":"10.1016/j.engreg.2023.01.003","DOIUrl":"https://doi.org/10.1016/j.engreg.2023.01.003","url":null,"abstract":"","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49152022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV-induced senescence of human dermal fibroblasts restrained by low-stiffness matrix by inhibiting NF-κB activation","authors":"Xuefeng Yao ,&nbsp;Huaqiong Li ,&nbsp;Liping Chen ,&nbsp;Lay Poh Tan","doi":"10.1016/j.engreg.2022.08.002","DOIUrl":"10.1016/j.engreg.2022.08.002","url":null,"abstract":"<div><p>As a hallmark of skin aging, senescent human dermal fibroblasts (HDFs) are known to lose the ability to divide. However, they can still interact with their cellular environment and the surrounding matrix. As the skin ages, the progressive slowing down of HDFs function decreases the skin's structural integrity, which is more serious than if there is the dermal collagen matrix eroded. This leads to matte<u>r</u>s of the unbalanced barrier under the skin, skin fragility, inadequate wound healing, as well as other cosmetic issues. It is also well documented that skin aging comes with significant stiffness increases. Therefore, understanding the interactions between HDFs and the surrounding microenvironments during senescence may provide insights into skin aging. Here we aim to investigate matrix stiffness' effect on HDF senescence and elucidate possible mechanisms that make HDFs senescent. In our experiments, HDFs were cultivated on Polydimethylsiloxane (PDMS) with various stiffnesses and exposed to UV light to trigger senescence. Results show that HDFs are significantly affected by senescence when cultured on a matrix with stiffness. However, the cells are not significantly affected when cultured on a low stiffness matrix. The following characterization revealed cells cultured on stiff substrates under UV exposure had stimulated the nucleus factor kappa-B (NF-<em>κ</em>B) activation. In contrast, cells on a matrix of softness only displayed low activation of NF-<em>κ</em>B. NF-<em>κ</em>B activity suppression with ammonium pyrrolidine dithiocarbamate (PDTC) decreases UV-induced HDFs senescence on stiff substrates. Taken together, we demonstrated that soft matrix defends HDFs against ultraviolet-induced senescence by inhibiting the activation of NF-<em>κ</em>B.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"3 4","pages":"Pages 365-373"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138122000536/pdfft?md5=511d88d2b1a21cc7e5e0cd3d62f0ceb2&pid=1-s2.0-S2666138122000536-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42658634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicking the native bone regenerative microenvironment for in situ repair of large physiological and pathological bone defects","authors":"Pei Wang ,&nbsp;Xiansong Wang","doi":"10.1016/j.engreg.2022.09.004","DOIUrl":"10.1016/j.engreg.2022.09.004","url":null,"abstract":"<div><p>Bone is a complex biological tissue with a complicated hierarchical nanocomposite structure. The native microenvironment of the bone tissue may be significantly disrupted by large physiological and pathological bone defects. Bone defects are often treated via complex surgical procedures that involve the application of autografts or allografts. While these grafting procedures often suffer from insufficient natural bone stock and immunorejection. Moreover, these traditional treatment methods fail to simulate a regenerative microenvironment, which plays a significant role in regeneration of bone tissue and repair of large bone defects. To this end, various biomimetic scaffolds have been devised to mimic the native microenvironment of bone and thereby to simultaneously repair bone defects and promote bone regeneration. We propose here a novel concept, <em>in vivo</em> bone regenerative microenvironment (BRM), which enables repair of large bone defects and enhances new bone tissue formation with external regulation. In this review, we mainly focus on materials and methods for fabrication of biomimetic scaffolds, as well as their therapeutic efficacy in modulating the BRM of large physiological and pathological bone defects<strong>.</strong></p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"3 4","pages":"Pages 440-452"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138122000603/pdfft?md5=5ea1ca9b5ad48fa40a1aba4eea8bf74e&pid=1-s2.0-S2666138122000603-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46599798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and biomechanical characterizations of acellular porcine mitral valve scaffolds: anterior leaflets, posterior leaflets, and chordae tendineae","authors":"Bo Wang ,&nbsp;Leslie N. Sierad ,&nbsp;Jeremy J. Mercuri ,&nbsp;Agneta Simionescu ,&nbsp;Dan T. Simionescu ,&nbsp;Lakiesha N. Williams ,&nbsp;Ryan Vela ,&nbsp;Pietro Bajona ,&nbsp;Matthias Peltz ,&nbsp;Sharan Ramaswamy ,&nbsp;Yi Hong ,&nbsp;Jun Liao","doi":"10.1016/j.engreg.2022.08.003","DOIUrl":"10.1016/j.engreg.2022.08.003","url":null,"abstract":"<div><p>Mitral valve (MV) tissue engineering is still in its early stage, and one major challenge in MV tissue engineering is to identify appropriate scaffold materials. With the potential of acellular MV scaffolds being demonstrated recently, it is important to have a full understanding of the biomechanics of the native MV components and their acellular scaffolds. In this study, we have successfully characterized the structural and mechanical properties of porcine MV components, including anterior leaflet (AL), posterior leaflet (PL), strut chordae, and basal chordae, before and after decellularization. Quantitative DNA assay showed more than 90% reduction in DNA content, and <em>Griffonia simplicifolia</em> (GS) lectin immunohistochemistry confirmed the complete lack of porcine α-Gal antigen in the acellular MV components. In the acellular AL and PL, the atrialis, spongiosa, and fibrosa trilayered structure, along with its ECM constitutes, i.e., collagen fibers, elastin fibers, and portion of GAGs, were preserved. Nevertheless, the ECM of both AL and PL experienced a certain degree of disruption, exhibiting a less dense, porous ECM morphology. The overall anatomical morphology of the strut and basal chordae were also maintained after decellularization, with longitudinal morphology experiencing minimum disruption, but the cross-sectional morphology exhibiting evenly-distributed porous structure. In the acellular AL and PL, the nonlinear anisotropic biaxial mechanical behavior was overall preserved; however, uniaxial tensile tests showed that the removal of cellular content and the disruption of structural ECM did result in small decreases in maximum tensile modulus, tissue extensibility, failure stress, and failure strain for both MV leaflets and chordae.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"3 4","pages":"Pages 374-386"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138122000548/pdfft?md5=1e76b3b8c50bf8ea4b20fc5dac1d60d0&pid=1-s2.0-S2666138122000548-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46689794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strontium doped electrospinning fiber membrane with antibacterial and osteogenic properties prepared by pulse electrochemical method","authors":"Yonggang Liu ,&nbsp;Bolin Zhang ,&nbsp;Feifei Liu ,&nbsp;Yani Qiu ,&nbsp;Wenbo Mu ,&nbsp;Liang Chen ,&nbsp;Chuang Ma ,&nbsp;Tingjun Ye ,&nbsp;Yingbo Wang","doi":"10.1016/j.engreg.2022.07.005","DOIUrl":"10.1016/j.engreg.2022.07.005","url":null,"abstract":"<div><p>The osteogenic differentiation and new bone formation are commonly delayed by bacterial infection of orthopedic implants, which is urgent to be resolved quickly in the clinic. The current paper prepared a strontium-doped electrospinning fiber membrane with antibacterial and osteogenic properties by pulse electrochemical method. Polylactic acid/hydroxyapatite (PLLA/HA) composite fiber substrate was fabricated by electrospinning technology, and strontium doped SrHA/Cu/Polypyrrole (PPy) composite coating was constructed with pulse electrodeposition method on its surface. The strontium doping technique, degradation of Sr²⁺ and Cu²⁺, cellular compatibility, and the antibacterial activity of the fiber membrane were examined. The results revealed that the deposition of phosphorus and calcium on composite fiber was the highest, indicating good biological activity. The release of Sr²⁺ and Cu²⁺ was stable and gradual due to the modulation of PPy. The composite fiber presented excellent antibacterial performance and the antibacterial rate was close to 100% against Staphylococcus aureus and Escherichia coli. Furthermore, it is conducive to the adhesion, spread, and proliferation of vascular endothelial cells and osteoblasts, namely outstanding osteogenesis and angiogenesis abilities. In conclusion, the multifunctional PLLA/HA@SrHA/Cu/PPy composite fiber membrane with good antibacterial and osteogenic activity by electrospinning technology and pulsed electrochemical deposition method provides an effective strategy for the poor bone healing of infected bone defects.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"3 4","pages":"Pages 339-351"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138122000469/pdfft?md5=f8fc8de754da3c0e3bbe185b6415be8c&pid=1-s2.0-S2666138122000469-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48925172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer","authors":"Xueqian Ren ,&nbsp;Liping Huang ,&nbsp;Chengde Wang ,&nbsp;Yuancai Ge ,&nbsp;Kaili Zhang ,&nbsp;Danfeng Jiang ,&nbsp;Xiaohu Liu ,&nbsp;Qingwen Zhang ,&nbsp;Yi Wang","doi":"10.1016/j.engreg.2022.08.004","DOIUrl":"10.1016/j.engreg.2022.08.004","url":null,"abstract":"<div><p>We developed a reliable simple analysis of adenosine, creatinine and other nucleosides (cytidine and guanosine) with single measurement based on surface-enhanced Raman spectroscopy (SERS) using DSP-AuNPs with Cu²⁺ ions probe and applied it to achieve noninvasive screening of lung cancer. The gold nanoparticles (AuNPs) were modified with 3,3′-dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP), which could react with above mentioned molecules followed with the aggregation of AuNPs at the presence of Cu²⁺, thus form the plasmonic hot spots to dramatically increase their fingerprint Raman signal. The probe of DSP-AuNPs was applied for the detail analysis of urinary adenosine of healthy people and lung cancer patients. The SERS measurement indicated a higher concentration of urinary adenosine for lung cancer patients than that of healthy people, which was consistent with the results measured by high-performance liquid chromatography (HPLC). In combination of Raman spectroscopy with the orthogonal partial least squares discriminant analysis (OPLS-DA) model, we are able to discriminate the lung cancer patients from healthy people with the urinary test. The results indicated that besides of adenosine, other metabolites including uric acid, guanine and creatinine may also be the potential tumor markers in urine for the noninvasive lung cancer diagnosis. Such a method paves a way for the noninvasive cancer screening and it can be further modified for the detection of other molecules on medical diagnosis.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"3 4","pages":"Pages 387-396"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266613812200055X/pdfft?md5=ff1dc897e1acdfe94fbbd9196760f854&pid=1-s2.0-S266613812200055X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45314484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered olfactory system for in vitro artificial nose","authors":"Tianshi Zhang ,&nbsp;Wenfei Ren ,&nbsp;Fangfang Xiao ,&nbsp;Jiguang Li ,&nbsp;Baiyi Zu ,&nbsp;Xincun Dou","doi":"10.1016/j.engreg.2022.09.003","DOIUrl":"10.1016/j.engreg.2022.09.003","url":null,"abstract":"<div><p>The engineered biomimetic sensors can not only realize the action of organs, but also combine functional materials as in vitro organs by simulating the response of biological organs to different environmental signals. Artificial nose is a concept proposed by imitating biological olfactory system, simulating olfactory nerve cells, olfactory bulb and olfactory cortex through different materials to realize olfactory function. The sensor array used to sense external gas stimulation can be analyzed based on different recognition principles through different original signals such as optics, electricity, electrochemistry and bioelectricity. Furthermore, combined with pattern recognition and microarray technology, artificial nose can be highly integrated with biocompatible and other important properties to achieve in vitro application. The design principle and necessary components of artificial nose are introduced in this paper including sensing structure, recognition system and functional module. At the same time, the potential development prospects of molecular recognition technology, polymer-based materials and microarray integration in artificial nose are prospected.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"3 4","pages":"Pages 427-439"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138122000597/pdfft?md5=6deba3f09131d0a4bf375fe77b9dda3f&pid=1-s2.0-S2666138122000597-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45121038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}