Engineered regeneration最新文献_第7页

Applications of silk-based biomaterials in biomedicine and biotechnology 丝基生物材料在生物医学和生物技术中的应用

Engineered regeneration Pub Date : 2023-12-14 DOI: 10.1016/j.engreg.2023.11.002

Punuri Jayasekhar Babu , Laldinthari Suamte

{"title":"Applications of silk-based biomaterials in biomedicine and biotechnology","authors":"Punuri Jayasekhar Babu , Laldinthari Suamte","doi":"10.1016/j.engreg.2023.11.002","DOIUrl":"https://doi.org/10.1016/j.engreg.2023.11.002","url":null,"abstract":"<div><p>Silk-based biomaterials have gained significant importance making them a promising choice for the future of medical technology due to their versatility and biocompatibility. They can be fabricated and tailored through various processing methods such as electrospinning, freeze-drying, and 3D printing, to achieve specific properties and structures namely sponges, hydrogels, films, and scaffolds that can be utilized for different biomedical applications. Biocompatibility, a unique property of silk-based biomaterials, has been demonstrated through both <em>in vivo</em> and <em>in vitro</em> studies and to date many studies have reported the successful use of these silk-based biomaterials in different fields of medicine. In this review, we have elaborately discussed different types of silk, their structural composition, and biophysical properties. Also, the current review focuses on highlighting various biomedical applications of engineered and fabricated silk-based biomaterials which aid in the treatment of certain infections and diseases related to skin, eyes, teeth, bone, heart, nerves, and liver. Furthermore, we have consolidated the advancements of silk-based biomaterials in the different fields of biotechnology such as sensors, food coating and packaging, textiles, drug delivery, and cosmetics. However, the research in this field continues to expand and more significant observations must be generated with feasible results for their reliable use in different biomedical applications.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 1","pages":"Pages 56-69"},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138123000609/pdfft?md5=0488dc2316efe71b2633d538a1be780f&pid=1-s2.0-S2666138123000609-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138657213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PETG: Applications in Modern Medicine PETG:现代医学的应用

Engineered regeneration Pub Date : 2023-11-25 DOI: 10.1016/j.engreg.2023.11.001

Catherine Yan , Corinne Kleiner , Aaron Tabigue , Veer Shah , Gregory Sacks , Darshi Shah , Vincent DeStefano

引用次数: 0

Fabrication and characterisation of random and aligned electrospun scaffolds to investigate hypothalamic stem/progenitor cell behaviour 随机排列电纺丝支架的制备和表征用于研究下丘脑干/祖细胞行为

Engineered regeneration Pub Date : 2023-10-27 DOI: 10.1016/j.engreg.2023.10.002

Selina Beal , Iain Stewart , Paul Hatton , Marysia Placzek , Ilida Ortega

{"title":"Fabrication and characterisation of random and aligned electrospun scaffolds to investigate hypothalamic stem/progenitor cell behaviour","authors":"Selina Beal , Iain Stewart , Paul Hatton , Marysia Placzek , Ilida Ortega","doi":"10.1016/j.engreg.2023.10.002","DOIUrl":"https://doi.org/10.1016/j.engreg.2023.10.002","url":null,"abstract":"<div><p>Tanycytes are stem/progenitor cells that reside in the hypothalamus of the adult vertebrate brain. Tanycytes can be cultured as free-floating neurospheres <em>in vitro</em> but tend to spontaneously differentiate over time. Here we asked whether morphological cues provided by engineered polymer scaffolds can modify spontaneous differentiation. Tanycyte-derived neurospheres were cultured on electrospun scaffolds, prepared with either random or aligned fiber morphologies. Cells dispersed widely on the scaffolds, and - on aligned scaffolds - were highly organized, orientated parallel to the fibers. Immunocytochemical analysis showed that cells cultured on aligned scaffolds showed significantly greater expression of the neural stem/progenitor cell marker, NrCAM and reduced expression of differentiated cell markers in comparison to those cultured on random scaffolds. Together this shows that tanycytes respond to local engineered cues, and that a morphologically constrained environment can better maintain tanycytes as stem cells. The aligned scaffold culture system provides a powerful tool to better investigate this novel stem/progenitor cell population.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 1","pages":"Pages 11-20"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266613812300052X/pdfft?md5=e4d415da26ebce844bd594a1de785963&pid=1-s2.0-S266613812300052X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92075557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding bone-inspired and cell-instructive cues of scaffolds for bone tissue engineering 破解骨组织工程支架的骨启发和细胞引导线索

Engineered regeneration Pub Date : 2023-10-26 DOI: 10.1016/j.engreg.2023.10.003

Zahid Hussain , Shah Mehmood , Xingzhu Liu , Yuanshan Liu , Guocheng Wang , Renjun Pei

{"title":"Decoding bone-inspired and cell-instructive cues of scaffolds for bone tissue engineering","authors":"Zahid Hussain , Shah Mehmood , Xingzhu Liu , Yuanshan Liu , Guocheng Wang , Renjun Pei","doi":"10.1016/j.engreg.2023.10.003","DOIUrl":"https://doi.org/10.1016/j.engreg.2023.10.003","url":null,"abstract":"<div><p>Bone fractures are common occurrence in clinical settings, creating a high demand for effective repair material. Unfortunately, limited graft availability, donor site morbidities, unpredictable clinical outcomes, immunologic reactions, infection risks, and geometrical mismatching concerns hampered tissue graft use and underscored the need for scaffolds for more effective bone reconstructions due to their tunable properties. Significant progress has been carried out in past decade in the fields of nanoceramics synthesis, bioconjugate chemistry, and composite material processing. This review outlines hierarchical structures and biology of bone tissue, materialistic components of scaffolds (bioceramics, polymers, bioactive drugs), featured scaffolding strategies (nanofibers, hydrogels, aerogels, bioprinting, and fiber-reinforced composite), and emphasis that hierarchical and physiochemical characteristics of bone should be used as an inspiration for scaffold design. This review discussed how differences in materiobiological aspects of scaffolds, such as polymer/bioceramic nanocomposite, mineralized nanocomposite, matrix-rich nanocomposite, 3D microenvironmental cues, pore space cues, mechanical cues, usage of physical stimulation (magnetic, electroactive, and photoactivated cues), surface cues (wettability, roughness, textured, and surface charge), and biointerface cues (cell–biomaterial interactions, cell-selective homing, and cell regulatory strategies) modulate cellular and biological response for bone tissue engineering. This study further outlines the challenges and benefits of integrating materiobiological cues of scaffolds for bone tissue engineering.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 1","pages":"Pages 21-44"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666138123000531/pdfft?md5=c76bd7184e1aee3a1ca2bd45d9834dbb&pid=1-s2.0-S2666138123000531-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92075556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically strong porous bioceramic tubes facilitate large segmental bone defect repair by providing long-term structurally stability and promoting osteogenesis 机械强度强的多孔生物陶瓷管通过提供长期结构稳定性和促进成骨促进大节段骨缺损修复

Engineered regeneration Pub Date : 2023-10-24 DOI: 10.1016/j.engreg.2023.10.001

Lijun Xie , Jiahao Zhang , Hangxiang Sun , Zehao Chen , Wangsiyuan Teng , Xupeng Chai , Cong Wang , Xianyan Yang , Yifan Li , Sanzhong Xu , Zhongru Gou , Zhaoming Ye

{"title":"Mechanically strong porous bioceramic tubes facilitate large segmental bone defect repair by providing long-term structurally stability and promoting osteogenesis","authors":"Lijun Xie , Jiahao Zhang , Hangxiang Sun , Zehao Chen , Wangsiyuan Teng , Xupeng Chai , Cong Wang , Xianyan Yang , Yifan Li , Sanzhong Xu , Zhongru Gou , Zhaoming Ye","doi":"10.1016/j.engreg.2023.10.001","DOIUrl":"https://doi.org/10.1016/j.engreg.2023.10.001","url":null,"abstract":"<div><p>Mechanically strong magnesium-doped Ca-silicate bioceramic scaffolds have many advantages in repairing large segmental bone defects. Herein we combine β-TCP with 6 mol% magnesium-doped calcium silicate (Mg6) at three different ratios (TCP, TCP+15 %Mg6, TCP+85 %Mg6) to find an appropriate ratio which can exert considerable influence on bone regeneration. In this study, the bioceramic scaffolds were assessed for mechanical strength, bioactive ion release, biocompatibility, and osteogenic capacity through <em>in vitro</em> testing. Additionally, the potential for promoting bone regeneration was investigated through <em>in vivo</em> implantation of porous tube-like scaffolds. The results showed that the compressive strength increased with the augmentation of Mg6 component. Especially the compressive strength of the TCP+85 %Mg6 group reached 38.1 ± 3.8 MPa, three times that of the other two groups. Furthermore, extensive <em>in vivo</em> investigations revealed that the TCP+85 %Mg6 bioceramic scaffolds were particularly beneficial for the osteogenic capacity of critical-sized femoral defects (20 mm in length). Altogether, magnesium doping in bioceramic implants is a promising strategy to provide stronger mechanical support and enhance osteogenesis to accelerate the repair of large defects.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 1","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71772982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered artificial skins: Current construction strategies and applications 工程人造皮肤:当前的施工策略和应用

Engineered regeneration Pub Date : 2023-09-30 DOI: 10.1016/j.engreg.2023.09.001

Ye Xu , Xiangyi Wu , Yuanyuan Zhang , Yunru Yu , Jingjing Gan , Qian Tan

{"title":"Engineered artificial skins: Current construction strategies and applications","authors":"Ye Xu , Xiangyi Wu , Yuanyuan Zhang , Yunru Yu , Jingjing Gan , Qian Tan","doi":"10.1016/j.engreg.2023.09.001","DOIUrl":"https://doi.org/10.1016/j.engreg.2023.09.001","url":null,"abstract":"<div><p>Skin damage resulting from burns, injuries, or diseases can lead to significant functional and esthetic deficits. However, traditional treatments, such as skin grafting, have limitations including limited donor skin availability, poor aesthetics, and functional impairment. Skin tissue engineering provides a promising alternative, with engineered artificial skins offering a highly viable avenue. Engineered artificial skin is designed to mimic or replace the functions of natural human skin and find applications in various medical treatments, particularly for severe burns, chronic wounds, and other skin injuries or defects. These artificial skins aim to promote wound healing, provide temporary coverage, permanent skin replacement, and restore the skin's barrier function. Artificial skins have diverse applications in medicine and wound care, addressing burns, chronic wounds, and traumatic injuries. They also serve as valuable tools for research in tissue engineering, offering experimental models for studying wound healing mechanisms, testing new biomaterials, and exploring innovative approaches to skin regeneration. This review provides an overview of current construction strategies for engineered artificial skin, including cell sources, biomaterials, and construction techniques. It further explores the primary application areas and future prospects of artificial skin, highlighting their potential to revolutionize skin reconstruction and advance the field of regenerative medicine.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 4","pages":"Pages 438-450"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Strategies for engineering neural cell alignment and their biomedical applications 神经细胞排列的工程策略及其生物医学应用

Engineered regeneration Pub Date : 2023-09-29 DOI: 10.1016/j.engreg.2023.09.002

Nan Xia , Rui Liu , Weiwei Chen , Dandan Wang , Lingyun Sun

引用次数: 0

Biomimetic extracellular vesicles for the tumor targeted treatment 用于肿瘤靶向治疗的仿生细胞外囊泡

Engineered regeneration Pub Date : 2023-09-05 DOI: 10.1016/j.engreg.2023.08.002

Ruolin Shi , An Zhan , Xinze Li , Bin Kong , Gaofeng Liang

引用次数: 0

Understanding the interplay between cell force and cell adhesion processes 了解细胞力和细胞粘附过程之间的相互作用

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.04.002

Peng Wang , Jie Li , Qiang Wei

{"title":"Understanding the interplay between cell force and cell adhesion processes","authors":"Peng Wang , Jie Li , Qiang Wei","doi":"10.1016/j.engreg.2023.04.002","DOIUrl":"10.1016/j.engreg.2023.04.002","url":null,"abstract":"<div><p>Cells, wrapped among their neighbors and surrounding extracellular matrix (ECM), form cell-cell adhesions and cell-ECM adhesions. Extracellular biophysical cues exert a far-reaching influence on a sweeping of cell behaviors, including signal transduction, gene expression, and fate determination. Cell-cell adhesions mediated by intercellular adhesion molecules bridge the membranes of adjacent cells through either heterophilic or homophilic adhesive interactions, playing a critical part in multicellular structural maintenance and, therefore, a foundation for multicellular organisms. Cell-ECM adhesions are derived from the interaction between cell adhesion receptors and multi-adhesive matrix proteins to ensure cell and tissue cohesion. Whereas cells not only unilaterally respond to certain cues from extracellular environment but can also alter the physicochemical profiles of the externalities and hence hold important implications for clinical applications. The essential function of cell adhesions has created tremendous interests in developing methods for measuring and studying cell adhesion properties, namely, cellular force. Here, we describe the collection of cell adhesive inputs on cellular signaling cascades and the “crosstalk” between cell-cell adhesions and cell-ECM adhesions. Furthermore, we provide the summary of the current methods to measure such cell adhesive forces.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 277-288"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43057832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rassf2 overexpression mediated by AAV promotes the supporting cell-to-hair cell transformation in the cochlea AAV介导的Rassf2过表达促进耳蜗支持细胞向毛细胞转化

Engineered regeneration Pub Date : 2023-09-01 DOI: 10.1016/j.engreg.2023.04.003

Liyan Zhang , Jieyu Qi , Yuan Fang , Fangzhi Tan , Yinyi Zhou , Ziyu Zhang , Qiuhan Sun , Nianci Li , Yideng Huang , Jingwu Sun , Renjie Chai

{"title":"Rassf2 overexpression mediated by AAV promotes the supporting cell-to-hair cell transformation in the cochlea","authors":"Liyan Zhang , Jieyu Qi , Yuan Fang , Fangzhi Tan , Yinyi Zhou , Ziyu Zhang , Qiuhan Sun , Nianci Li , Yideng Huang , Jingwu Sun , Renjie Chai","doi":"10.1016/j.engreg.2023.04.003","DOIUrl":"10.1016/j.engreg.2023.04.003","url":null,"abstract":"<div><p>Sensory hair cells are responsible for detecting and transmitting sound in the inner ear, and damage to HCs leads to hearing loss. HCs do not regenerate spontaneously in adult mammals, which makes the hearing loss permanent. However, hair cells and supporting cells have the same precursors in the inner ear, and in newborn mice, the adjacent SCs can be activated by gene manipulation to differentiate into newly regenerated hair cells. Here, we demonstrate the role of the Ras association domain family member 2 (Rassf2) in supporting cell to hair cell trans-differentiation in the inner ear. Using the AAV vector (AAV-ie) to upregulate Rassf2 expression promoted supporting cell division and hair cell production in cultured cochlear organoids. Also, AAV-Rassf2 enhanced the regenerative ability of Lgr5<sup>+</sup> SCs in the postnatal cochlea without impairing hearing, and this might due to the modulation of the Wnt, Hedgehog and Notch signaling pathways. Furthermore, AAV-Rassf2 enhances cochlear supporting cell division and hair cell production in the neomycin injury model. In summary, our results suggest that Rassf2 is a key component in HC regenerative repair, and gene modulation mediated by adeno-associated virus may be a promising gene therapy for hearing repair.</p></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"4 3","pages":"Pages 304-315"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41273788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0