Engineered regeneration最新文献_第6页

Engineering neuroblastoma models for clinical translation 用于临床翻译的神经母细胞瘤工程模型

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-06-06 DOI: 10.1016/j.engreg.2025.05.002

Taiyu Song , Rui Liu , Feika Bian , Bin Kong , Jingjing Gan

{"title":"Engineering neuroblastoma models for clinical translation","authors":"Taiyu Song , Rui Liu , Feika Bian , Bin Kong , Jingjing Gan","doi":"10.1016/j.engreg.2025.05.002","DOIUrl":"10.1016/j.engreg.2025.05.002","url":null,"abstract":"<div><div>Neuroblastoma is a profoundly heterogenous extracranial solid tumor in pediatric patients. Elevated risk grade of neuroblastoma has been correlated with unsatisfactory prognosis and resistance to chemotherapy. Despite multimodal therapies exploited for killing neuroblastoma tumor cells, in high-risk neuroblastoma patients, the long-term survival is currently less than 50%. Promising approaches to evaluating the extent of heterogeneity via gene expression profiling of cell subpopulations within individual tumors are still lacking. There is uncertainty about the cross-talk between neuroblastoma cells and other non-neoplastic cell components in the tumor microenvironment. Recently, concerns about individualized eradication therapies have advanced the demand for the diversified construction of neuroblastoma tumor models. This review briefly introduces the genetic variation, subpopulations, and tumor microenvironment aspects of neuroblastoma heterogeneity. Then, we summarize recent methods of constructing tumor models to mimic the biological features of neuroblastoma tumors <em>in vitro</em>. Finally, we conclude the future trends and perspectives in neuroblastoma tumor therapy.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 1","pages":"Pages 146-159"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted drug delivery system for Golgi apparatus's diseases 针对高尔基体疾病的靶向给药系统

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2024-06-08 DOI: 10.1016/j.engreg.2024.06.001

Yongxin Xu , Yi Jin , Yuting Huang , Ya Wen , Zhifeng Gu , Yujuan Zhu

{"title":"Targeted drug delivery system for Golgi apparatus's diseases","authors":"Yongxin Xu , Yi Jin , Yuting Huang , Ya Wen , Zhifeng Gu , Yujuan Zhu","doi":"10.1016/j.engreg.2024.06.001","DOIUrl":"10.1016/j.engreg.2024.06.001","url":null,"abstract":"<div><div>Golgi apparatus (GA) is an organelle widely present in eukaryotic cells and involved in a variety of cellular physiological activities, including but not limited to protein modification and secretion. There is increasing evidence that structural or functional disorders of the GA are closely associated with the occurrence and development of diseases. As potential therapeutic targets, researchers have developed GA-targeted drug delivery systems (DDS) for disease treatment. Compared with traditional therapy, DDS achieves remarkable curative effect with high specificity, low dose, reduced drug resistance and side effects, via the alterations in GA morphology or biosynthesis. Therefore, GA-targeted therapy is of great clinical significance and has broad application prospects. In this review, the structure and function of GA are briefly introduced, and mechanisms of DDS entering cells and binding to the GA is classified. Then the typical applications of GA-targeted DDS in the diagnosis and treatment of cancer, cardiovascular diseases, fibrosis, infectious diseases and neurodegenerative diseases is introduced in detail, displaying its great potential in disease treatment. At last, the bottlenecks and future development of this field are discussed. It is our hope that this review will inspire the development of GA-based DDS for clinical applications in the foreseeable future.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 17-33"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141408168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel microsphere scaffold-based islet organoids for rescuing type 1 diabetes and reversing hyperglycemia 新型微球支架胰岛类器官用于治疗1型糖尿病和逆转高血糖

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-05-27 DOI: 10.1016/j.engreg.2025.05.001

Yanan Jing , Guidan Wang , Ruolin Shi , Wenjing Wen , Wenjie Wang , Xuan Zhao , Gaofeng Liang

{"title":"Novel microsphere scaffold-based islet organoids for rescuing type 1 diabetes and reversing hyperglycemia","authors":"Yanan Jing , Guidan Wang , Ruolin Shi , Wenjing Wen , Wenjie Wang , Xuan Zhao , Gaofeng Liang","doi":"10.1016/j.engreg.2025.05.001","DOIUrl":"10.1016/j.engreg.2025.05.001","url":null,"abstract":"<div><div>Type 1 diabetes (T1D) is an autoimmune deficiency disease characterized by elevated blood sugar levels and insulin resistance, leading to various adverse health effects and complications, such as diabetic cardiomyopathy and diabetic ketoacidosis. Currently, T1D is primarily treated through organoid transplantation and extracorporeal insulin injection. However, the clinical utility of these treatments is limited by increased systemic immunosuppression due to graft donor shortages and the side effects associated with exogenous insulin therapy. Recently, the emergence of bioengineered islet-like organs has opened up possibilities for constructing insulin-secreting cells in vitro to treat insulin-dependent diabetes. In this study, we developed a novel microsphere scaffold-based islet cell spheroid culture system that integrates islet organoids with 3D microsphere scaffolds, enabling the consistent generation of 3D islet cell spheroids. Following transplantation into the renal capsule of diabetic mice, these organoids demonstrated significant hypoglycemic effects, with detectable insulin secretion in the serum. On day 30 post-transplantation, β-cell marker expression was significantly increased in the grafts. We further investigated the glucose-related proteins that microsphere scaffold-based islet organoids may regulate. Our findings confirm that islet-like organoids can effectively secrete insulin and play a role in maintaining blood sugar stability. These results indicate that islet-like organs generated via microsphere scaffolds exhibit similar endocrine functions to those of natural islets, can survive in the host body for extended periods, and can effectively exert hypoglycemic effects, thereby providing a solid foundation for the application of islet-like organs in type 1 diabetes research.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 1","pages":"Pages 121-132"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive scaffolds integrated with micro-precise spatiotemporal delivery and in vivo degradation tracking for complex tissue regeneration 生物活性支架集成微精确时空传递和体内降解跟踪复杂组织再生

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-01-07 DOI: 10.1016/j.engreg.2025.01.001

Hun Jin Jeong, Alia Koch, Soomin Park, Solaiman Tarafder, Chang H. Lee

{"title":"Bioactive scaffolds integrated with micro-precise spatiotemporal delivery and in vivo degradation tracking for complex tissue regeneration","authors":"Hun Jin Jeong, Alia Koch, Soomin Park, Solaiman Tarafder, Chang H. Lee","doi":"10.1016/j.engreg.2025.01.001","DOIUrl":"10.1016/j.engreg.2025.01.001","url":null,"abstract":"<div><div>Three-dimensional (3D) printing has evolved to incorporate controlled delivery systems to guide the regeneration of complex tissues, with limited clinical translation. The challenges include the limited precision in spatiotemporal delivery and poorly understood <em>in vivo</em> scaffold degradation rates. Here, we report auspicious preclinical outcomes in the functional regeneration of temporomandibular joint (TMJ) discs of mini-pigs. TMJ disc has been an extremely challenging target for regenerative engineering given the uniquely heterogeneous matrix distribution and region-variant anisotropic orientation. We optimally implemented advanced 3D printing technologies with micro-precise spatiotemporal delivery to build anatomically correct, bioactive scaffolds with native-like regionally variant microstructure and mechanical properties. We also applied quantum dots (QDs) labeling of scaffolds to enable non-invasive <em>in vivo</em> degradation tracking. In mini-pigs, the scaffold implantation upon discectomy has successfully led to <em>in-situ</em> regeneration of TMJ discs by 3 months, exhibiting native-like heterogeneity and multi-scale mechanical properties without any sign of cartilage damage. In addition, our non-invasive imaging resulted in reliable <em>in vivo</em> tracking of scaffold degradation, exhibiting notably different degradation rates between individual animals. Our findings suggest a significant translational potential of our cell-free, bioactive scaffolds equipped with non-invasive tracking modality for in-situ tissue engineering of TMJ discs.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 34-44"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel microsphere scaffold-based islet organoids for rescuing type 1 diabetes and reversing hyperglycemia 新型微球支架胰岛类器官用于治疗1型糖尿病和逆转高血糖

Engineered regeneration Pub Date : 2025-01-01 Epub Date: 2025-05-27 DOI: 10.1016/j.engreg.2025.05.001

Yanan Jing , Guidan Wang , Ruolin Shi , Wenjing Wen , Wenjie Wang , Xuan Zhao , Gaofeng Liang

{"title":"Novel microsphere scaffold-based islet organoids for rescuing type 1 diabetes and reversing hyperglycemia","authors":"Yanan Jing , Guidan Wang , Ruolin Shi , Wenjing Wen , Wenjie Wang , Xuan Zhao , Gaofeng Liang","doi":"10.1016/j.engreg.2025.05.001","DOIUrl":"10.1016/j.engreg.2025.05.001","url":null,"abstract":"<div><div>Type 1 diabetes (T1D) is an autoimmune deficiency disease characterized by elevated blood sugar levels and insulin resistance, leading to various adverse health effects and complications, such as diabetic cardiomyopathy and diabetic ketoacidosis. Currently, T1D is primarily treated through organoid transplantation and extracorporeal insulin injection. However, the clinical utility of these treatments is limited by increased systemic immunosuppression due to graft donor shortages and the side effects associated with exogenous insulin therapy. Recently, the emergence of bioengineered islet-like organs has opened up possibilities for constructing insulin-secreting cells in vitro to treat insulin-dependent diabetes. In this study, we developed a novel microsphere scaffold-based islet cell spheroid culture system that integrates islet organoids with 3D microsphere scaffolds, enabling the consistent generation of 3D islet cell spheroids. Following transplantation into the renal capsule of diabetic mice, these organoids demonstrated significant hypoglycemic effects, with detectable insulin secretion in the serum. On day 30 post-transplantation, β-cell marker expression was significantly increased in the grafts. We further investigated the glucose-related proteins that microsphere scaffold-based islet organoids may regulate. Our findings confirm that islet-like organoids can effectively secrete insulin and play a role in maintaining blood sugar stability. These results indicate that islet-like organs generated via microsphere scaffolds exhibit similar endocrine functions to those of natural islets, can survive in the host body for extended periods, and can effectively exert hypoglycemic effects, thereby providing a solid foundation for the application of islet-like organs in type 1 diabetes research.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"6 ","pages":"Pages 121-132"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “The Artificial Disc Nucleus and Other Strategies for Replacement of the Nucleus Pulposus: Past, Present and Future Designs for an Emerging Surgical Solution” [Engineered Regeneration 5(2024), 269-281] “人工椎间盘核和其他替代髓核的策略：一种新兴手术解决方案的过去、现在和未来设计”的勘误表[工程再生5(2024),269-281]

Engineered regeneration Pub Date : 2024-12-01 Epub Date: 2024-12-14 DOI: 10.1016/j.engreg.2024.12.001

Greg Sacks, Vincent DeStefano, Claire Parker, Ryan Lebens, Harry Mushlin

引用次数: 0

Cochlear implant/MXene-based electroacoustic stimulation modulates the growth and maturation of spiral ganglion neurons 基于人工耳蜗/MXene的电声刺激可调节螺旋神经节神经元的生长和成熟

Engineered regeneration Pub Date : 2024-12-01 Epub Date: 2024-10-11 DOI: 10.1016/j.engreg.2024.10.001

Yangnan Hu , Hao Wei , Menghui Liao , Shanying Han , Xin Gao , Yusong Wang , Shan Zhou , Dongyu Xu , Xugang Zhuang , Ye Yang , Hong Cheng , Bin Zhang , Qingyue Cui , Jieyu Qi , Lei Tian , Wenyan Li , Xia Gao , Renjie Chai

引用次数: 0

Asymmetric porous composite hydrogel patch for microenvironment-adapted repair of contaminated abdominal wall defects 非对称多孔复合水凝胶补片用于微环境适应性修复污染性腹壁缺损

Engineered regeneration Pub Date : 2024-12-01 Epub Date: 2024-04-09 DOI: 10.1016/j.engreg.2024.03.004

Yang Yu , Yinxiang Tang , Weiwen Liang , Yuanbin Wang , Yang Ouyang , Wenxuan Xiong , Bingna Zheng , Lili Chu , Hui Wang

{"title":"Asymmetric porous composite hydrogel patch for microenvironment-adapted repair of contaminated abdominal wall defects","authors":"Yang Yu , Yinxiang Tang , Weiwen Liang , Yuanbin Wang , Yang Ouyang , Wenxuan Xiong , Bingna Zheng , Lili Chu , Hui Wang","doi":"10.1016/j.engreg.2024.03.004","DOIUrl":"10.1016/j.engreg.2024.03.004","url":null,"abstract":"<div><div>Effective antibacterial property and long-term mechanical support are essential for the repair of complex abdominal wall defects associated with infection. However, clinically available repair materials often fail to meet these requirements, resulting in high surgical failure rate and complications. In this study, an asymmetric porous composite hydrogel patch (cCS/PVA@BAC) with antibacterial, anti-adhesion, pro-healing, and durable mechanical support properties is designed for the efficient repair of contaminated abdominal wall defects. By stepwise phase-conversion and soaking method, robust and stable polyvinyl alcohol hydrogel (PVAH) is integrated with the biocompatible multicomponent hydrogel made of chitosan and carboxymethyl chitosan (cCS), and benzalkonium chloride (BAC) is loaded to enhance the antibacterial property. The cCS layer of cCS/PVA@BAC has an extracellular matrix-like porous structure, which can promote fibroblasts adhesion and wound healing. In contrast, the PVAH layer on the other side with a smooth and dense structure, which can reduce fibroblasts adhesion and prevent visceral adhesion. In addition, the composite hydrogel patch has good anti-swelling and anti-deformation properties as well as stable mechanical strength, thus can withstand high intraperitoneal pressure in the wet internal microenvironment. The loaded BAC can efficiently kill bacteria and improve the local inflammatory microenvironment. With these advantages, cCS/PVA@BAC can significantly reduce inflammation, promote tissue remodeling, and accelerate the healing of contaminated abdominal wall defects in the rat model. These findings suggest a potential use of multifunctional hydrogel patch as an ideal material for effective repair of contaminated soft tissue defects.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 4","pages":"Pages 468-481"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140756844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A patch comprising human umbilical cord-derived hydrogel and mesenchymal stem cells promotes pressure ulcer wound healing 由人脐带水凝胶和间充质干细胞组成的贴片可促进压疮伤口愈合

Engineered regeneration Pub Date : 2024-12-01 Epub Date: 2024-10-12 DOI: 10.1016/j.engreg.2024.10.002

Liqin Chen , Ying Zhang , Kexin Wang , Meixian Jin , Qi Chen , Simin Wang , Wei Hu , Zhai Cai , Yang Li , Shao Li , Yi Gao , Shuqin Zhou , Qing Peng

{"title":"A patch comprising human umbilical cord-derived hydrogel and mesenchymal stem cells promotes pressure ulcer wound healing","authors":"Liqin Chen , Ying Zhang , Kexin Wang , Meixian Jin , Qi Chen , Simin Wang , Wei Hu , Zhai Cai , Yang Li , Shao Li , Yi Gao , Shuqin Zhou , Qing Peng","doi":"10.1016/j.engreg.2024.10.002","DOIUrl":"10.1016/j.engreg.2024.10.002","url":null,"abstract":"<div><div>Pressure ulcers (PUs) are common skin injuries known for their high morbidity, rapid onset, susceptibility to infection, and challenging healing process. One potential therapy for PUs is cell-based therapy using mesenchymal stem cells (MSCs). However, poor survival and low cell retention of MSCs on skin lesions limit their therapeutic effects and applications. In this study, we prepared an extracellular matrix (dECM) hydrogel decellularized from the human umbilical cord (UC). A patch composed of UC-dECM and UC-MSCs was employed in the treatment of PUs in C57BL/6 mice. Our results indicate that the UC-dECM hydrogel effectively sustains cell viability, enhances the stemness-related gene expression in UC-MSCs, and promotes human umbilical vein endothelial cells (HUVECs) migration and angiogenesis. Compared to the groups treated with the patch containing only UC-dECM, injection of UC-MSCs or gauze dressing, the patch combining UC-dECM hydrogel with UC-MSCs significantly accelerated PU healing. This positive outcome can be attributed to the promotion of tissue re-epithelialization, collagen deposition, angiogenesis, and inflammation inhibition. Our results suggest that the composite patch, comprised of UC-dECM hydrogel and UC-MSCs, may be a promising therapeutic approach for PU treatment.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 4","pages":"Pages 433-442"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone improvement in osteoporotic rabbits using CoCrMo implants 使用钴铬钼合金植入体改善骨质疏松兔子的骨质

Engineered regeneration Pub Date : 2024-12-01 Epub Date: 2024-05-18 DOI: 10.1016/j.engreg.2024.05.002

Jésica I. Zuchuat , Adriana S. Manzano , Valeria Sigot , Gastón L. Miño , Oscar A. Decco

{"title":"Bone improvement in osteoporotic rabbits using CoCrMo implants","authors":"Jésica I. Zuchuat , Adriana S. Manzano , Valeria Sigot , Gastón L. Miño , Oscar A. Decco","doi":"10.1016/j.engreg.2024.05.002","DOIUrl":"10.1016/j.engreg.2024.05.002","url":null,"abstract":"<div><div>The management of bone repair in patients with osteoporosis depends on the clinical situation and the extent of the damage. The repair of bone lesions by inducing new bone formation is important for maintaining bone architecture and density. Herein, we reported the use of Cobalt Chromium Molybdenum (CoCrMo) implants in osteoporotic rabbits and the regenerative outcomes <em>in vivo</em>. The aim was to determine whether the placement of CoCrMo plates would induce qualitative and quantitative differences in the osteoporotic tissue beneath and surrounding the implant. We assessed the effect of the alloy in the bone of animals receiving implants for 4 and 8 weeks and compared the results to those of the osteoporotic non-implanted bone and the healthy controls. After 4 weeks, minimal histological changes were observed, whereas after 8 weeks a marked osteogenesis was evident with both apposition and substitution of new bone. In addition, a greater number of Haversian canals with increased canal area and decreased intracortical pores were observed in the implanted <em>vs</em> non implanted limb for both experimental groups. We show for the first time that the use of CrCoMo plates induces bone formation under osteoporotic conditions. The beneficial effect is localised on the cortical bone in areas in contact with the material. Although this effect may not directly influence the OP disease itself, it has direct implications for new bone formation adjacent to the biomaterial. This potential enhancement could play a crucial role in improving implant fixation in compromised bone, offering increased biocompatibility and stability.</div></div>","PeriodicalId":72919,"journal":{"name":"Engineered regeneration","volume":"5 4","pages":"Pages 495-504"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141133439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0