Current research in physiology最新文献

{"title":"Verifying the physiological significance of the preference for low facial temperature in humans","authors":"Mayumi Matsuda-Nakamura ,&nbsp;Satoshi Wada ,&nbsp;Kei Nagashima","doi":"10.1016/j.crphys.2025.100176","DOIUrl":"10.1016/j.crphys.2025.100176","url":null,"abstract":"<div><h3>Objectives</h3><div>Regional differences in thermal pleasantness have been reported; local cooling of the head induces pleasantness during mild heat exposure. However, the physiological significance of these effects remains unclear. This study tested the hypothesis that facial or neck cooling could selectively decrease brain temperature (selective brain cooling; SBC).</div></div><div><h3>Methods</h3><div>Eight male volunteers participated in normothermic and hyperthermic protocols, comprising a 10-min baseline, 15-min cooling, and 15-min post-cooling period. During cooling, facial fanning (FAN_face), forehead conductive cooling (COND_head), neck conductive cooling (COND_neck), or control without cooling (NO-C) was performed. In the hyperthermic protocol, the participants performed cycling exercises at 60 % heart rate reserve until oesophageal temperature (T_es) rose by 1 °C. The difference between tympanic temperature (T_ty) and T_es was used as an index of brain cooling.</div></div><div><h3>Results</h3><div>Under normothermic conditions, no significant difference was observed between T_ty and T_es in any trial. Under hyperthermic conditions, T_ty was significantly lower than T_es after facial fanning, with the largest difference 10 min after the end of facial fanning (T_ty = 37.6 ± 0.3 °C; T_es = 37.7 ± 0.3 °C; <em>P</em> = 0.001, Cohen's <em>d</em> = 2.021); the brain-cooling index (T_es–T_ty) was 0.08 ± 0.04 °C. No significant differences occurred in the NO-C, COND_head, or COND_neck trials. These results suggest that facial fanning should contribute slightly to brain cooling in hyperthermic individuals.</div></div><div><h3>Conclusion</h3><div>A preference for low facial temperature should help cool the brain. However, this brain cooling effect should be minimal compared with SBC in animals possessing carotid rete. SBC in humans could not be validated by the results in this study.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"9 ","pages":"Article 100176"},"PeriodicalIF":1.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145938509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex differences in brachial and superficial femoral artery occlusion pressure","authors":"Pat R. Vehrs ,&nbsp;Shay Richards ,&nbsp;Joshua Allen ,&nbsp;Tyler Burbank ,&nbsp;Stephanie D. Gardner ,&nbsp;Ryan B. Lacey ,&nbsp;Jessica H. Watkin ,&nbsp;Jackson Passey ,&nbsp;Gregory L. Snow ,&nbsp;Ron L. Hager","doi":"10.1016/j.crphys.2026.100182","DOIUrl":"10.1016/j.crphys.2026.100182","url":null,"abstract":"<div><div>It is well accepted that arterial occlusion pressure (AOP) of the arm and leg should be measured prior to blood flow restriction (BFR) training. Although limb circumference is a key determinant of AOP, there is mounting evidence suggestive of sex differences in AOP.</div></div><div><h3>Objective</h3><div>To further clarify sex differences and variables predictive of AOP in the arms and legs.</div></div><div><h3>Methods</h3><div>Using ultrasound, we measured AOP of the brachial artery (80 males; 83 females) and the superficial femoral artery (84 males; 80 females) in the dominant limbs. We also evaluated independent variables predictive of AOP.</div></div><div><h3>Results</h3><div>Independent <em>t</em>-tests revealed sex differences in brachial artery AOP (128 ± 13.1 mmHg; 116 ± 9.9 mmHg; <em>p</em> &lt; 0.001) but not superficial femoral artery AOP (204.7 ± 31.8 mmHg; 203.9 ± 36.1 mmHg; <em>p</em> = 0.8871) between males and females, respectively. Regression analysis identified systolic blood pressure (SBP) and arm circumference to be predictive of brachial artery AOP (p &lt; 0.0001; R² = 0.6345) while SBP, diastolic blood pressure, limb circumference, skinfold thickness, vessel diameter, and blood flow were predictive of superficial femoral artery AOP (p &lt; 0.0001; R² = 0.6149). Sex was not predictive of AOP.</div></div><div><h3>Conclusions</h3><div>Brachial artery AOP is significantly greater in males than in females. Limb circumference alone was a poor predictor of AOP in the arm and leg. Because there is a diverse set of variables that are predictive of AOP, to ensure its safe and effective use, the AOP of each limb should be measured prior to BFR training.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"9 ","pages":"Article 100182"},"PeriodicalIF":1.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How effective is online teaching of physiology modules? perception of Rwandan health sciences students","authors":"Abdullateef Isiaka Alagbonsi ,&nbsp;Mojeed Akorede Gbadamosi ,&nbsp;Mathieu Nemerimana ,&nbsp;Makinde V. Olubiyi ,&nbsp;Olufunke Onaadepo ,&nbsp;Akaninyene Ubong Ime ,&nbsp;Abdul-Rahuf Aderemi Feyitimi ,&nbsp;Ruth Wanjiru Mungai ,&nbsp;Florent Rutagarama ,&nbsp;Danilo Milanes Zambrano ,&nbsp;Noah Adavize Omeiza ,&nbsp;Simiat Olanike Elias","doi":"10.1016/j.crphys.2025.100181","DOIUrl":"10.1016/j.crphys.2025.100181","url":null,"abstract":"<div><h3>Background</h3><div>While blended teaching is approved in Rwandan medical education, the perceived effectiveness of online-only delivery for physiology modules remains unclear. This uncertainty limits its continued use. The present study assessed Rwandan health sciences students’ perceptions of the effectiveness of online teaching for physiology modules.</div></div><div><h3>Methods</h3><div>A total of 528 students from medicine and surgery (n = 300), pharmacy (n = 99), dentistry (n = 89), and clinical psychology (n = 40) programs at the University of Rwanda completed an online questionnaire. Data were analyzed using multivariable logistic regression and non-parametric tests (Mann-Whitney U, Kruskal-Wallis) with corresponding effect sizes.</div></div><div><h3>Results</h3><div>Female students (adjusted odds ratio (aOR) = 0.64, 95 % CI: 0.43–0.94, <em>p</em> = 0.024) had significantly lower odds of perceiving online teaching as effective, whereas Year 2 students (aOR = 1.63, 95 % CI: 1.05–2.55, <em>p</em> = 0.030) had significantly higher odds of doing so. Experiential factors strongly influenced perceived effectiveness: satisfaction with online teaching (aOR = 35.11, 95 % CI: 6.96–177.11, <em>p</em> &lt; 0.001), high interactivity (aOR = 5.17, 95 % CI: 2.46–10.87, <em>p</em> &lt; 0.001), and regular participation in discussions (aOR = 7.58, 95 % CI: 3.47–16.55, <em>p</em> &lt; 0.001) were associated with positive perceptions. Video conferencing was rated as most helpful for complex concepts (Kruskal-Wallis H (5) = 65.58, <em>p</em> &lt; 0.001). The predictive model (Nagelkerke R² = 0.629) strongly indicated that students perceived online teaching as comparably effective as face-to-face teaching.</div></div><div><h3>Conclusions</h3><div>Students’ perceptions of online teaching effectiveness are shaped more by interactivity, satisfaction, and active participation than by demographic characteristics. Educators should prioritize interactive, synchronous sessions and foster student engagement to increase acceptance of online physiology teaching in Rwanda.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"9 ","pages":"Article 100181"},"PeriodicalIF":1.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145646003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of safranal on anxiety and the MYD88/NF-kb signaling pathway in the hippocampus in a rat model of ulcerative colitis","authors":"Kaveh Rahimi,&nbsp;Atena Kooti,&nbsp;Nirvana Kafaie,&nbsp;Asal Chaboksavarian,&nbsp;Mohammad Javad Alimohammadi","doi":"10.1016/j.crphys.2025.100177","DOIUrl":"10.1016/j.crphys.2025.100177","url":null,"abstract":"<div><h3>Introduction</h3><div>Individuals with colitis are more likely to experience anxiety symptoms compared to those without the condition. However, the biological mechanisms linking colitis to anxiety remain unclear. This study investigated the potential effects of safranal on anxiety-like behaviors and the involvement of the MYD88/NF-κB signaling cascade within the hippocampus in a rat model of ulcerative colitis induced by acetic acid.</div></div><div><h3>Methods</h3><div>Thirty-two male rats were randomly divided into four groups: a control group, a colitis group, and two treatment groups receiving safranal at 72.75 or 145.5 mg/kg. The intervention lasted seven days. To confirm colitis induction, the Disease Activity Index (DAI) was assessed based on weight loss, stool consistency, and presence of blood. Anxiety-like behaviors were evaluated using the open field test and elevated plus maze. Molecular analyses measured hippocampal expression of TLR4, MYD88, and NF-κB, along with IL-6 and IL-1β protein levels.</div></div><div><h3>Results</h3><div>Safranal administration alleviated anxiety-like behaviors, as reflected by improved performance in both behavioral tests. Colitis induction was confirmed by a significant elevation in the DAI score, while safranal treatment at both doses significantly reduced this score, indicating attenuation of colitis severity. Safranal also decreased hippocampal IL-1β and IL-6 levels and suppressed TLR4, MYD88, and NF-κB expression, demonstrating inhibition of neuroinflammatory signaling.</div></div><div><h3>Conclusions</h3><div>These findings suggest that safranal exerts anxiolytic effects in experimental colitis, likely mediated through downregulation of the TLR4/MYD88/NF-κB pathway and reduced hippocampal neuroinflammation.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"9 ","pages":"Article 100177"},"PeriodicalIF":1.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of troxerutin on schizophrenia-like behaviors and oxidative damage in mice","authors":"Parham Malek ,&nbsp;Shahin Hassanpour ,&nbsp;Morteza Zendehdel","doi":"10.1016/j.crphys.2026.100183","DOIUrl":"10.1016/j.crphys.2026.100183","url":null,"abstract":"<div><div>The aim of this study was to investigate the influence of troxerutin on ketamine-induced schizophrenia-like behaviors in male mice, as well as its effects on antioxidant levels in brain tissue and serum cytokines. There were four experimental groups, each consisting of six male mice. The control group did not receive any treatment, the ketamine group was administered ketamine (30 mg/kg, ip), the troxerutin group was given troxerutin (150 mg/kg, orally), and the combined group was treated with both ketamine (30 mg/kg, ip) and troxerutin (150 mg/kg, orally). The animals were given troxerutin for a period of 30 days. Ketamine was injected once a day between days 16 and 30 of the study. At the end of the study, symptoms similar to schizophrenia were evaluated using novel object recognition, tail suspension, forced swimming, and open field assessments. Furthermore, measurements were taken of serum levels of TNF-α and IL-1β. Subsequently, animals were euthanized and the concentrations of malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) were assessed in both the brain cortex and sub-cortex. According to the results, ketamine resulted in a significant decrease in body weight and the frequency of crossings in the open field assay, while enhancing immobility duration in the tail suspension and forced swim assessments (P &lt; 0.05). Furthermore, ketamine significantly heightened memory deficits (P &lt; 0.05). Pre-treatment with troxerutin significantly reduced the effects of ketamine on body weight, open field test outcomes, and immobility duration (P &lt; 0.05). The results indicated that troxerutin can protect the brain from ketamine-induced issues in mice by reducing behavioral deficits, oxidative stress, and serum levels of inflammatory cytokines.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"9 ","pages":"Article 100183"},"PeriodicalIF":1.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle activity of the lower extremities during exergames with varying movement demands in older adults (Registered Report - Stage I).","authors":"Thomas C Kosiolek, Dirk Möller, Eleftheria Giannouli","doi":"10.1016/j.crphys.2025.100158","DOIUrl":"10.1016/j.crphys.2025.100158","url":null,"abstract":"<p><strong>Objective: </strong>This study examines lower-leg muscle activity during four exergames with varying movement demands in older adults. A secondary objective is to compare individual muscle activity across the exergames.</p><p><strong>Background: </strong>Exergaming, which combines physical exercise with interactive video games, is increasingly used in older adults' rehabilitation, improving balance, strength, and cognitive function. Prior research suggests that different exergames engage specific motor functions. However, the activity of key lower-leg muscles-critical for balance-has not been systematically analyzed across exergames with varying movement demands (e.g., weight shifts, multidirectional steps, stepping in place, and walking).</p><p><strong>Methods: </strong>This cross-sectional study investigates muscle activity of the M. tibialis anterior, M. gastrocnemius, and M. soleus during four exergames using surface EMG. Muscle activity is quantified as Root Mean Square (RMS 100) values and normalized to the maximum voluntary contraction (MVC). Exergames are performed on the Dividat Senso platform, each emphasizing different movement patterns: multidirectional stepping, balance shifting, stepping in place, and whole-platform stepping. Thirty older adults (≥65 years) with no major functional or cognitive impairments (SPPB ≥7, MoCA ≥26) will be recruited. Exclusion criteria include severe motor impairments, mobility restrictions, extreme visual impairments, unstable cardiovascular conditions, or language barriers. Muscle activity will be compared across exergames using Multivariate Analysis of Variance (MANOVA), followed by univariate ANOVAs to identify specific differences.</p><p><strong>Potential significance: </strong>This study will identify muscle-specific activity patterns during exergames, informing targeted rehabilitation and exergame design. Findings may improve training strategies for balance enhancement and fall prevention in older adults, with potential benefits for healthcare cost reduction and quality of life.</p>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"100158"},"PeriodicalIF":1.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The beneficial effect of propolis during an eight-weeks of resistance training on TNF-α and IL-6 genes expression in the kidney tissue of female Wistar rats undergoing testosterone enanthate consumption","authors":"Khadijeh Molaei ,&nbsp;Sanaz Mirzayan Shanjani ,&nbsp;Ali Gorzi ,&nbsp;Yaser Kazemzadeh ,&nbsp;Abdolali Banaeifar","doi":"10.1016/j.crphys.2025.100167","DOIUrl":"10.1016/j.crphys.2025.100167","url":null,"abstract":"<div><div>This study investigated the potential benefits of propolis in mitigating the adverse effects of testosterone enanthate on the gene expression levels of pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) in the kidney tissue of female Wistar rats undergoing resistance training. Testosterone enanthate, a commonly used anabolic steroid among female athletes, is known to impair kidney function through inflammatory and oxidative pathways. Twenty-four female Wistar rats (8 weeks old, weighing 208.22 ± 14.17 g) were obtained from the Pasteur Institute. After a one-week acclimatization period, the rats were randomly divided into three groups: 1) training + placebo (n = 8), 2) training + testosterone enanthate (n = 8), and 3) training + testosterone enanthate + propolis (n = 8). The 8-week resistance training program involved a 1-m vertical ladder with 26 steps and additional weights. Training occurred five days a week with two rest days. Rats in the testosterone enanthate group received injections of 20 mg/kg body weight. The other group received the same testosterone supplementation plus propolis via gavage at 400 mg/kg body weight, three times a week. TNF-α gene expression in the training + testosterone group was significantly higher than in the training + placebo (<em>P</em> &lt; 0.0001) and training + testosterone + propolis (<em>P</em> = 0.0025) groups. TNF-α gene expression in the training + testosterone + propolis group was also significantly higher than in the training + placebo group (<em>P</em> = 0.0001). Similarly, IL-6 gene expression in the training + testosterone group was significantly higher than in both the training + placebo (<em>P</em> &lt; 0.0001) and training + testosterone + propolis (<em>P</em> = 0.0142) groups. IL-6 gene expression in the training + testosterone + propolis group was significantly higher than in the training + placebo group (<em>P</em> = 0.0016). These findings suggest that propolis supplementation can partially attenuate the inflammatory effects of testosterone enanthate on kidney tissue, likely through modulation of cytokines gene expression, highlighting its potential therapeutic role in preserving kidney health during anabolic steroid exposure.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"Article 100167"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145323271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-generated multiple-choice questions in health science education: Stakeholder perspectives and implementation considerations","authors":"Matthew Reid ,&nbsp;Michelle French ,&nbsp;Stavroula Andreopoulos ,&nbsp;Christine Wong ,&nbsp;Nohjin Kee","doi":"10.1016/j.crphys.2025.100160","DOIUrl":"10.1016/j.crphys.2025.100160","url":null,"abstract":"<div><div>Multiple-choice questions (MCQs) are widely used in health science education because they are an efficient way to evaluate knowledge from simple recall to complex clinical reasoning. The creation of high-quality MCQs, however, can be time-consuming and requires expertise in question composition. Advancements in artificial intelligence (AI), especially large language models (LLMs), offer the potential to allow for the rapid generation of high-quality, consistent, and course-specific MCQs. Here we discuss the potential benefits and drawbacks of the use of this technology in the generation of MCQs, including ensuring the accuracy and fairness of questions, along with technical, ethical, and privacy considerations. We offer practical guiding principles for the implementation of AI-generated MCQs and outline future research areas related to their impact on student learning and educational quality.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"Article 100160"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"You don't know what you don't know; using high school outreach to improve awareness of bioscience-based careers and higher education","authors":"Christine Greensmith ,&nbsp;David Greensmith","doi":"10.1016/j.crphys.2025.100151","DOIUrl":"10.1016/j.crphys.2025.100151","url":null,"abstract":"<div><div>When considering the diversity of students in higher education, an important but frequently overlooked characteristic is socioeconomic background. It is well known that those from socially disadvantaged backgrounds are less likely to progress to higher education, and accounts for an associated lack of diversity in the STEM workplace. The reasons for this are many and complex, though a lack of awareness of STEM-based education thence career pathways among secondary school learners remains a key contributor. To address this, we designed an adaptable and scalable high school outreach programme that sought to raise STEM (with a focus on bioscience) awareness through events that could be readily adapted to meet high school needs and resource constraints. Learner perceptions were recorded using a pre- and post-event questionnaire. Following the event, awareness of bioscience-based careers and the required prerequisite skills and qualifications were increased by 58 and 53 % respectively. The degree to which learners were considering a bioscience-based career was increased by 43 %. Though interest in attending university to study STEM was unaltered, awareness of the qualifications required to progress to university was increased by 58 %. These findings suggest that outreach events are an effective way to raise general awareness of STEM-based higher education learning thence careers and highlight the importance of tailoring outreach events to meet school and learner needs.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"Article 100151"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential responses to acute endurance exercise-induced signaling protein phosphorylation and abundance alterations in whole murine gastrocnemius muscle and isolated myofibers","authors":"Kazuki Uemichi ,&nbsp;Takanaga Shirai ,&nbsp;Shunsuke Sugiyama ,&nbsp;Tohru Takemasa","doi":"10.1016/j.crphys.2025.100173","DOIUrl":"10.1016/j.crphys.2025.100173","url":null,"abstract":"<div><div>Skeletal muscle is composed of diverse cell types, including myofibers. In this study, we investigated how acute endurance exercise could induce signaling protein phosphorylation and abundance alterations using protein lysate extracted from whole-muscle tissue or isolated myofibers. We subjected 8-week-old male Institute Cancer Research mice to a single bout of treadmill running-based endurance exercise, and evaluated the signaling protein expression responses in the whole gastrocnemius muscle and isolated myofibers, obtained by incubation in 0.2 % collagenase/medium, immediately after exercise. Our results revealed that exercise activated mitochondrial biogenesis- and protein anabolism-related signaling, including 5′-adenosine monophosphate-activated kinase and Akt/p70 S6 kinases, respectively, as well as increased glucose metabolism-related proteins in whole-muscle samples immediately after exercise. However, these signaling responses were not present in the muscle fiber lysate and mitochondrial oxidative phosphorylation-related protein abundance significantly decreased postexercise. Our data suggest that signaling protein activation and quantitative alterations in myofibers digested in collagenase-dissolved medium post acute exercise do not necessarily reflect the expression responses in whole skeletal muscle tissue.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"Article 100173"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145518951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}