Advances in biochemical engineering/biotechnology最新文献

{"title":"Correction to: In Situ Online Spectroscopy and Light Scattering Techniques.","authors":"Hévila Brognaro, Christian Betzel","doi":"10.1007/10_2026_345","DOIUrl":"10.1007/10_2026_345","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"423"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classical and Nonclassical Nucleation Mechanisms of Biomolecules.","authors":"Yevgeniya Karibjanova, Sébastien Teychené, Isaac Rodriguez-Ruiz","doi":"10.1007/10_2025_306","DOIUrl":"10.1007/10_2025_306","url":null,"abstract":"<p><p>This chapter offers a critical review of classical and nonclassical nucleation mechanisms in protein crystallization. It focuses on two-step nucleation (TSN) and composite cluster models as alternatives to classical nucleation theory (CNT), highlighting their relevance for describing nucleation in systems where intermediate disordered phases emerge. A particular emphasis is placed on the role of liquid-liquid phase separation (LLPS) as a modulator of the local environment and a potential precursor state. Through experimentally documented case studies, the spatial location of nucleation events - within dense phases, at interfaces, or in the lean phase - is discussed in relation to structural and kinetic parameters. Emphasis is placed on the role of intermolecular interactions, phase behavior, and limitations of current models and measurement techniques. Finally, recent advances in multidimensional free energy landscapes and simulation approaches are discussed, highlighting the need for integrated experimental and computational strategies to better understand and control nucleation in complex biological systems.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"139-175"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioreactor Design and Engineering for Cultivated Meat Manufacturing.","authors":"Marie-Luise Schlieker, David Vorländer, Laura Pasitka, Sebastian Bode, Tatjana Krampitz, Chantal Treinen, Marius Henkel","doi":"10.1007/10_2026_314","DOIUrl":"10.1007/10_2026_314","url":null,"abstract":"<p><p>Bioreactors encompass a wide spectrum of engineering concepts, yet for decades, industrial biotechnology has seen little fundamental innovation, with large-scale production mainly confined to stirred-tank and bubble-column reactors for microbial fermentations. In pharmaceutical biotechnology, various cultivation systems for eukaryotic cells are well established, including microcarrier processes, suspension in stirred vessels, and disposable bags or wave (rocking) bioreactors. However, these systems have typically been optimized to produce specific biomolecules, such as recombinant proteins, vaccines, or monoclonal antibodies, rather than for generating cellular biomass itself. The emergence of tissue engineering for medical applications brought new technological impulses, particularly in the development of scaffold materials and the spatial organization of cells in three-dimensional constructs. This was accompanied by advances in perfusion bioreactors, enabling sustained nutrient delivery and waste removal within thick tissue cultures. Such systems were designed for relatively small, high-value applications in regenerative medicine, but they provide valuable conceptual and technical groundwork for structured cultivated meat manufacturing. In cellular agriculture, the goal is to apply and adapt established bioprocessing knowledge to a new challenge: producing animal cells at food-relevant scales and costs. Rather than high-value, low-volume pharmaceutical production, the focus shifts to high-volume, cost-efficient food production. Achieving this will require interdisciplinary collaboration between bioprocess engineers, automation specialists, tissue engineers, cell biologists, and food technologists.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"81-153"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External Fields as Control Strategies to Promote Biomolecule Nucleation.","authors":"Rohan P Y van Tooren, Hüseyin Burak Eral","doi":"10.1007/10_2025_311","DOIUrl":"10.1007/10_2025_311","url":null,"abstract":"<p><p>Acoustic, electric, and magnetic external fields have been extensively investigated as a promising non-invasive approach to control nucleation from solution. Among these approaches, light fields leveraging lasers such as non-photochemical laser-induced nucleation (NPLIN) have seen a recent surge of interest. NPLIN involves an interaction between the solute-solvent system and laser light, but takes place at wavelengths and intensities not known to invoke any photochemical phenomena. Promising applications include direct enhancement of the nucleation rate, as well as providing polymorphic control and spatial precision in initiating crystallisation. This chapter mainly reviews the current state of NPLIN research focusing on biomolecules. Secondly, this chapter discusses other external-field-based methods, albeit in much less detail. The chapter concludes with the authors' perspective on future directions of research dedicated towards biomolecule crystal nucleation using such field-based methods.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"251-269"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life Cycle Analysis for Agricultural Residues and Related Products.","authors":"Penjit Srinophakun, Anusith Thanapimmetha, Nutchapon Chiarasumran, Khemmathin Lueangwattanapong, Maythee Saisriyoot","doi":"10.1007/10_2026_327","DOIUrl":"10.1007/10_2026_327","url":null,"abstract":"<p><p>Agricultural residues represent an abundant and underutilized resource that can be redirected from disposal pathways toward value-added applications such as composting, mulching, bioenergy, land amendment, and bioplastics. This chapter examines these pathways through the perspective of life cycle assessment (LCA), highlighting how agricultural residues can contribute to waste reduction, soil improvement, greenhouse gas mitigation, and circular resource use. Across the reviewed case studies, environmental outcomes are shown to depend not only on the residue itself, but also on the selected conversion pathway, management practice, system boundary, and local implementation context. Composting and mulching can reduce environmental burdens, although benefits remain sensitive to process modifications, additive use, and field conditions. Energy recovery from residues can offer substantial gains when carbon-intensive reference systems are displaced, but performance varies with logistics and conversion efficiency. Land application may improve soil quality and carbon retention while also shifting greenhouse gas fluxes depending on amendment type and site conditions. Likewise, residue-derived bioplastics can lower impacts relative to fossil-based plastics under favorable feedstock and end-of-life scenarios, but these benefits are not universal. Overall, the chapter shows that LCA is a valuable tool for comparing residue management options while also emphasizing the need for integrated assessment frameworks that account for environmental, technical, and implementation-related factors when selecting sustainable valorization strategies.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"363-385"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Microbial Strains for Co-fermentation of C5 and C6 Sugars to Produce Cellulosic Ethanol.","authors":"Ryo Nasuno, Cai-Yun Xie, Yoshiaki Kawahara, Yue-Qin Tang, Tomohisa Hasunuma","doi":"10.1007/10_2026_319","DOIUrl":"10.1007/10_2026_319","url":null,"abstract":"<p><p>Efficient fermentation of both hexose (C6) and pentose (C5) sugars derived from lignocellulosic biomass is essential for the sustainable production of cellulosic ethanol. However, conventional yeast Saccharomyces cerevisiae lacks native pathways for pentose utilization and is susceptible to various environmental stresses caused by hydrolysate-rich fermentation conditions. This chapter outlines the metabolic engineering strategies for introducing and optimizing C5 pathways alongside native C6 metabolism. It highlights advances in recombinant strain development, including synthetic pathway design, cofactor balancing, and transporter engineering. We also review approaches for consolidated bioprocessing that improve overall productivity by combining saccharification and fermentation in a single batch. These approaches include the secretion or cell surface display of cellulases and hemicellulases and optimizing their expression levels. Furthermore, this chapter introduces tolerance mechanisms against stresses derived from fermentation conditions including high temperature, osmolarity, and ethanol, as well as lignocellulose hydrolysate-derived inhibitors such as acetic acid and furfural. By connecting innovations in metabolic design and stress response, the chapter provides a comprehensive view of the current progress and future directions in developing yeast strains to co-ferment C5/C6 sugars under industrially practical conditions.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"139-182"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenging Pretreatment Technologies of Lignocellulosic Biomass.","authors":"Xinchuan Yuan, Guannan Shen, Qianqian Yang, Yin Cheng, Yiwei Chang, Yao Chen, Xingye Hu, Rui Zhai, Mingjie Jin","doi":"10.1007/10_2026_317","DOIUrl":"10.1007/10_2026_317","url":null,"abstract":"<p><p>Lignocellulosic biomass, derived from agricultural and forestry waste, is a promising renewable resource for biofuels and biochemicals. However, its recalcitrant structure, which is comprised of intertwined cellulose, hemicellulose, and lignin, hinders efficient conversion, necessitating pretreatment as a crucial step to disrupt this matrix and enhance enzymatic hydrolysis. Pretreatment methods are categorized based on their target components: hemicellulose depolymerization strategies such as acid pretreatment (e.g., inorganic, organic, and solid acids) and physicochemical methods like steam explosion improve sugar release but face challenges like inhibitor formation and high energy costs; lignin removal strategies, including alkaline, oxidative, and biological pretreatments, effectively degrade lignin yet struggle with reagent recovery and low working efficiency; and selective fractionation strategies, such as organosolv, ionic liquids, and deep eutectic solvents, aim for high-value utilization but encounter issues with solvent sustainability and costs. Emerging technologies like dry acid pretreatment with biodetoxification (DryPB), ammonia-based methods, and densifying lignocellulosic biomass with chemicals (DLC) offer improved efficiency, reduced waste, and high product yields, highlighting the need for future optimization in cost-effectiveness, selectivity, and integration for scalable biorefineries.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"79-111"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148012673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomolecular Crystallisation Through Soft Templates and Seeding.","authors":"Jerry Heng, Vivek Verma, Hamish Mitchell, Daniele Pessina","doi":"10.1007/10_2025_310","DOIUrl":"10.1007/10_2025_310","url":null,"abstract":"<p><p>The crystallisation of macromolecules is largely dependent on the generation of supersaturation while also maintaining conditions that minimally disrupt their natural molecular conformation in solution. While conventional methods of generating supersaturation typically involve either lowering the temperature of the crystallisation solution or allowing solution evaporation to increase concentration, this is often unsuccessful in obtaining macromolecular crystals. This is because their flexible nature necessitates relatively higher energy to overcome the thermodynamic barrier to nucleation. Seeding presents itself as an innovative approach to mitigate this energy barrier and generate high-quality macromolecular crystals. This method involves utilising either macromolecular crystalline seeds, dissolved additives (soft templates), or undissolved additives (hard templates). The underlying principles of these methods primarily rely on three fundamental mechanisms: functional group matching, epitaxy, and topographical effects, all of which are extensively discussed in this chapter. Over the past two decades, there has been a significant enhancement in the success of macromolecular crystallisation, largely attributed to advancements in seeding and templating strategies. This chapter provides a comprehensive summary of the research conducted on seeding and templating to enhance the crystallisation capabilities of macromolecules, as well as the development of purification protocols for these intricate systems.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"303-339"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flow as a Control Strategy to Promote Biomolecule Nucleation.","authors":"Christo Nanev, Emmanuel Saridakis, Naomi E Chayen","doi":"10.1007/10_2025_308","DOIUrl":"10.1007/10_2025_308","url":null,"abstract":"<p><p>The main aim of this chapter is to provide an (at least semiquantitative) explanation of biomolecular crystallization (nucleation and growth) experimental results in solution flow, in order to further assist with optimization of the process. An overview will be provided of biomolecular crystal nucleation and growth in forced solution flows, but also in the presence and absence of natural (buoyancy-driven) convective flows. The chapter focuses first on the theoretical analysis of the different mass transfer modalities, diffusion versus convection dominated (both of crystal building blocks and of impurities), leading to biomolecule-depleted and to self-purifying (i.e., impurity-depleted) zones. The same topics will then be addressed, describing the experimentally investigated influence of forced flows, artificially created through air pressure, peristaltic pumping, oscillatory mixing, stirring, shearing, and other methods, both in small-scale laboratory and in large-scale industrial settings. In addition to forced flows, work on the influence of gravity-induced naturally occurring solution convection and crystal sedimentation will be reviewed, as well as the effects of its absence when crystallization trials are carried out under microgravity environments. By developing the principle of the separation of the nucleation and growth stages of crystallization (\"double-pulse technique\") not only in time but also in space, the reported results have shown that at solution flow velocities larger than those of buoyancy-driven convection, most crystals nucleated heterogeneously, while bulk-nucleated crystals predominated at lower flow velocities. Lastly, fluid flow also allowed the investigation of the important role of impurities of biological origin on the kind of crystal nucleation, homogeneous or heterogeneous. In view of the diverse needs for protein crystals (structure determination by conventional X-ray crystallography or by XFEL, industrial production of pharmaceuticals), flow-utilizing strategies may be adjusted to each specific crystallization need. Experiments could also be undertaken that would optimize the flow parameters separately and successively for each stage of the crystallization process (nucleation, growth, cessation of growth). Finally, it would be extremely informative to widen the range of proteins used in crystallization experiments involving flow, besides the usual lysozyme and a handful of others that have already been studied.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"209-250"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Other Techniques.","authors":"Ana M Gonçalves, Marino F A Santos, Luís A Passarinha","doi":"10.1007/10_2026_313","DOIUrl":"10.1007/10_2026_313","url":null,"abstract":"<p><p>The production of protein crystals suitable for high-resolution structural analysis critically depends on the quality, homogeneity, and functional integrity of the protein sample throughout the crystallization workflow. This chapter provides a comprehensive overview of key biochemical and biophysical parameters that govern successful protein crystallization, including protein purity, conformational and compositional homogeneity, structural integrity, and biological activity. We discuss the application of different complementary techniques - including chromatographic methods, mass spectrometry, circular dichroism, surface plasmon resonance, and X-ray crystallography - to systematically assess and optimize these parameters from initial sample preparation to final crystal formation and subsequent X-ray data collection. By integrating orthogonal characterization approaches, common issues such as aggregation, degradation, conformational heterogeneity, and loss of function can be identified and mitigated prior to crystallization trials, thereby improving reproducibility and diffraction quality. Furthermore, the chapter highlights alternative X-ray data collection methodologies such as room-temperature, in situ, and automated data collection that further enhances crystallographic workflows by reducing crystal handling, improving experimental efficiency, and enabling the capture of biologically relevant conformational states.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"271-319"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147626923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}