Advances in biochemical engineering/biotechnology最新文献_第3页

Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing. 合理设计用于蛋白质传感的 DNA 支架和切换探针。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_235

Alejandro Chamorro, Marianna Rossetti, Neda Bagheri, Alessandro Porchetta

{"title":"Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing.","authors":"Alejandro Chamorro, Marianna Rossetti, Neda Bagheri, Alessandro Porchetta","doi":"10.1007/10_2023_235","DOIUrl":"10.1007/10_2023_235","url":null,"abstract":"The detection of a protein analyte and use of this type of information for disease diagnosis and physiological monitoring requires methods with high sensitivity and specificity that have to be also easy to use, rapid and, ideally, single step. In the last 10 years, a number of DNA-based sensing methods and sensors have been developed in order to achieve quantitative readout of protein biomarkers. Inspired by the speed, specificity, and versatility of naturally occurring chemosensors based on structure-switching biomolecules, significant efforts have been done to reproduce these mechanisms into the fabrication of artificial biosensors for protein detection. As an alternative, in scaffold DNA biosensors, different recognition elements (e.g., peptides, proteins, small molecules, and antibodies) can be conjugated to the DNA scaffold with high accuracy and precision in order to specifically interact with the target protein with high affinity and specificity. They have several advantages and potential, especially because the transduction signal can be drastically enhanced. Our aim here is to provide an overview of the best examples of structure switching-based and scaffold DNA sensors, as well as to introduce the reader to the rational design of innovative sensing mechanisms and strategies based on programmable functional DNA systems for protein detection.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"71-106"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139562731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resource-Efficient Use of Residues from Medicinal and Aromatic Plants for Production of Secondary Plant Metabolites. 资源高效利用药用和芳香植物残留物生产植物次生代谢物。

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Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2024_250

Sibylle Kümmritz, Nanina Tron, Martin Tegtmeier, Axel Schmidt, Jochen Strube

引用次数: 0

Plant In Vitro Culture Factories for Pentacyclic Triterpenoid Production. 生产五环三萜类化合物的植物体外培养工厂。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_245

Ilian Badjakov, Ivayla Dincheva, Radka Vrancheva, Vasil Georgiev, Atanas Pavlov

{"title":"Plant In Vitro Culture Factories for Pentacyclic Triterpenoid Production.","authors":"Ilian Badjakov, Ivayla Dincheva, Radka Vrancheva, Vasil Georgiev, Atanas Pavlov","doi":"10.1007/10_2023_245","DOIUrl":"10.1007/10_2023_245","url":null,"abstract":"Pentacyclic triterpenoids are a diverse subclass of naturally occurring terpenes with various biological activities and applications. These compounds are broadly distributed in natural plant resources, but their low abundance and the slow growth cycle of plants pose challenges to their extraction and production. The biosynthesis of pentacyclic triterpenoids occurs through two main pathways, the mevalonic acid (MVA) pathway and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, which involve several enzymes and modifications. Plant in vitro cultures, including elicited and hairy root cultures, have emerged as an effective and sustainable system for pentacyclic triterpenoid production, circumventing the limitations associated with natural plant resources. Bioreactor systems and controlling key parameters, such as media composition, temperature, light quality, and elicitor treatments, have been optimized to enhance the production and characterization of specific pentacyclic triterpenoids. These systems offer a promising bioprocessing tool for producing pentacyclic triterpenoids characterized by a low carbon footprint and a sustainable source of these compounds for various industrial applications.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"17-49"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139690959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress on the Electrochemical Sensing of Illicit Drugs. 非法药物的电化学传感研究进展。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_239

Robin Van Echelpoel, Florine Joosten, Marc Parrilla, Karolien De Wael

引用次数: 0

Co-cultures from Plants and Cyanobacteria: A New Way for Production Systems in Agriculture and Bioprocess Engineering. 植物与蓝藻共培养：农业和生物加工工程生产系统的新途径。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_247

D Strieth, J Kollmen, J Stiefelmaier, A Mehring, R Ulber

{"title":"Co-cultures from Plants and Cyanobacteria: A New Way for Production Systems in Agriculture and Bioprocess Engineering.","authors":"D Strieth, J Kollmen, J Stiefelmaier, A Mehring, R Ulber","doi":"10.1007/10_2023_247","DOIUrl":"10.1007/10_2023_247","url":null,"abstract":"Due to the global increase in the world population, it is not possible to ensure a sufficient food supply without additional nitrogen input into the soil. About 30-50% of agricultural yields are due to the use of chemical fertilizers in modern times. However, overfertilization threatens biodiversity, such as nitrogen-loving, fast-growing species overgrow others. The production of artificial fertilizers produces nitrogen oxides, which act as greenhouse gases. In addition, overfertilization of fields also releases ammonia, which damages surface waters through acidification and eutrophication. Diazotrophic cyanobacteria, which usually form a natural, stable biofilm, can fix nitrogen from the atmosphere and release it into the environment. Thus, they could provide an alternative to artificial fertilizers. In addition to this, biofilms stabilize soils and thus protect against soil erosion and desiccation. This chapter deals with the potential of cyanobacteria as the use of natural fertilizer is described. Possible partners such as plants and callus cells and the advantages of artificial co-cultivation will be discussed later. In addition, different cultivation systems for studying artificial co-cultures will be presented. Finally, the potential of artificial co-cultures in the agar industry will be discussed.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"83-117"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139574290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid-State Nanopores for Biomolecular Analysis and Detection. 用于生物分子分析和检测的固态纳米孔。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_240

Annina Stuber, Tilman Schlotter, Julian Hengsteler, Nako Nakatsuka

{"title":"Solid-State Nanopores for Biomolecular Analysis and Detection.","authors":"Annina Stuber, Tilman Schlotter, Julian Hengsteler, Nako Nakatsuka","doi":"10.1007/10_2023_240","DOIUrl":"10.1007/10_2023_240","url":null,"abstract":"Advances in nanopore technology and data processing have rendered DNA sequencing highly accessible, unlocking a new realm of biotechnological opportunities. Commercially available nanopores for DNA sequencing are of biological origin and have certain disadvantages such as having specific environmental requirements to retain functionality. Solid-state nanopores have received increased attention as modular systems with controllable characteristics that enable deployment in non-physiological milieu. Thus, we focus our review on summarizing recent innovations in the field of solid-state nanopores to envision the future of this technology for biomolecular analysis and detection. We begin by introducing the physical aspects of nanopore measurements ranging from interfacial interactions at pore and electrode surfaces to mass transport of analytes and data analysis of recorded signals. Then, developments in nanopore fabrication and post-processing techniques with the pros and cons of different methodologies are examined. Subsequently, progress to facilitate DNA sequencing using solid-state nanopores is described to assess how this platform is evolving to tackle the more complex challenge of protein sequencing. Beyond sequencing, we highlight the recent developments in biosensing of nucleic acids, proteins, and sugars and conclude with an outlook on the frontiers of nanopore technologies.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"283-316"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139562792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chimeric Protein Switch Biosensors. 嵌合蛋白开关生物传感器。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_241

Emma Campbell, Timothy Luxton, Declan Kohl, Sarah A Goodchild, Christoph Walti, Lars J C Jeuken

{"title":"Chimeric Protein Switch Biosensors.","authors":"Emma Campbell, Timothy Luxton, Declan Kohl, Sarah A Goodchild, Christoph Walti, Lars J C Jeuken","doi":"10.1007/10_2023_241","DOIUrl":"10.1007/10_2023_241","url":null,"abstract":"Rapid detection of protein and small-molecule analytes is a valuable technique across multiple disciplines, but most in vitro testing of biological or environmental samples requires long, laborious processes and trained personnel in laboratory settings, leading to long wait times for results and high expenses. Fusion of recognition with reporter elements has been introduced to detection methods such as enzyme-linked immunoassays (ELISA), with enzyme-conjugated secondary antibodies removing one of the many incubation and wash steps. Chimeric protein switch biosensors go further and provide a platform for homogenous mix-and-read assays where long wash and incubation steps are eradicated from the process. Chimeric protein switch biosensors consist of an enzyme switch (the reporter) coupled to a recognition element, where binding of the analyte results in switching the activity of the reporter enzyme on or off. Several chimeric protein switch biosensors have successfully been developed for analytes ranging from small molecule drugs to large protein biomarkers. There are two main formats of chimeric protein switch biosensor developed, one-component and multi-component, and these formats exhibit unique advantages and disadvantages. Genetically fusing a recognition protein to the enzyme switch has many advantages in the production and performance of the biosensor. A range of immune and synthetic binding proteins have been developed as alternatives to antibodies, including antibody mimetics or antibody fragments. These are mainly small, easily manipulated proteins and can be genetically fused to a reporter for recombinant expression or manipulated to allow chemical fusion. Here, aspects of chimeric protein switch biosensors will be reviewed with a comparison of different classes of recognition elements and switching mechanisms.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"1-35"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139562686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imprinted Polymers on the Route to Plastibodies for Biomacromolecules (MIPs), Viruses (VIPs), and Cells (CIPs). 用于生物大分子（MIPs）、病毒（VIP）和细胞（CIPs）的质体抗体途径上的印迹聚合物。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_234

Xiaorong Zhang, Aysu Yarman, Mahdien Bagheri, Ibrahim M El-Sherbiny, Rabeay Y A Hassan, Sevinc Kurbanoglu, Armel Franklin Tadjoung Waffo, Ingo Zebger, Tutku Ceren Karabulut, Frank F Bier, Peter Lieberzeit, Frieder W Scheller

引用次数: 0

Recent Developments and Applications of Microbial Electrochemical Biosensors. 微生物电化学生物传感器的最新发展和应用。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_236

Nunzio Giorgio G Carducci, Sunanda Dey, David P Hickey

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Signal-Amplified Nanobiosensors for Virus Detection Using Advanced Nanomaterials. 利用先进纳米材料检测病毒的信号放大纳米生物传感器

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Advances in biochemical engineering/biotechnology Pub Date : 2024-01-01 DOI: 10.1007/10_2023_244

Akhilesh Babu Ganganboina, Enoch Y Park

引用次数: 0