Advances in biochemical engineering/biotechnology最新文献_第3页

Vitamin B₁₂ Biomanufacturing. 维生素B12生物制造。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-07-30 DOI: 10.1007/10_2026_338

Dawei Zhang, Huiying Wang, Qian Kang

{"title":"Vitamin B12 Biomanufacturing.","authors":"Dawei Zhang, Huiying Wang, Qian Kang","doi":"10.1007/10_2026_338","DOIUrl":"https://doi.org/10.1007/10_2026_338","url":null,"abstract":"Vitamin B12 (cobalamin), as an essential cobalt-containing vitamin for the human body, boasts significant physiological functions and broad application in the pharmaceutical, animal feed, food, and cosmetic industries. It mainly exists in four forms: adenosylcobalamin, methylcobalamin, cyanocobalamin, and hydroxocobalamin (hydroxycobalamin). Cyanocobalamin, due to its chemical stability, serves as the primary industrial product form. Currently, microbial fermentation stands as the core method for industrial vitamin B12 production, with strains such as Pseudomonas denitrificans and Propionibacterium freudenreichii. Nevertheless, the production remains relatively low, which has become a key constraint for the industry's development. This chapter highlights the principal innovations and notable accomplishments of the research team, led by Zhang Dawei from the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, in the domain of vitamin B12 biomanufacturing. By systematically reviewing the synthesis methods of vitamin B12, characteristics of industrial fermentation strains, metabolic engineering strategies, and fermentation technologies, this chapter clarifies the technical advantages and current development status of this field. Meanwhile, it analyzes the challenges encountered in the biomanufacturing process and the progress made in addressing them. Finally, it provides an overview of the global market pattern of vitamin B12 and the development trends of cost control. This comprehensive and in-depth review is expected to help readers gain a thorough and in-depth understanding of the vitamin B12 biomanufacturing.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the Enzymic Synthesis of Unnatural Chiral Amino Acids. 酶法合成非天然手性氨基酸的研究进展。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-07-30 DOI: 10.1007/10_2026_339

Yuxin Chen, Shizhen Wang

{"title":"Advances in the Enzymic Synthesis of Unnatural Chiral Amino Acids.","authors":"Yuxin Chen, Shizhen Wang","doi":"10.1007/10_2026_339","DOIUrl":"https://doi.org/10.1007/10_2026_339","url":null,"abstract":"Unnatural chiral amino acids (UCAAs), including D-amino acids and noncanonical L-amino acids, are immensely valuable chiral chemicals, which are extensively applied in pharmaceuticals, materials, and fine chemicals. Biosynthesis of UCAAs by enzymes has emerged as a powerful, green, and highly efficient strategy. Amino acid dehydrogenases, which catalyze asymmetric reductive amination of keto acids, represent the most widely employed enzyme for the synthesis of UCAAs. Transaminases, which enable asymmetric transfer of an amino group to keto acids, have also been successfully applied in the synthesis of several noncanonical L-amino acids. Furthermore, catalytic promiscuity of enzyme which considerably expanded the synthetic potential toward diverse UCAAs has been reported. In addition, multienzyme system with cofactor regeneration has significantly improved the synthesis efficiency. This review summarizes recent advances in the in vitro enzymatic synthesis recently published by Chinese researcher. An outlook on AI-driven biocatalysis for the green and sustainable production of UCAAs has been promoted. Emerging tools such as artificial intelligence, machine learning, and high-throughput platforms will further drive the green and efficient synthesis of chiral amino acids.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Biomanufacturing of D-Glucaric Acid. d -葡萄糖酸的绿色生物制造。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-07-30 DOI: 10.1007/10_2026_335

Hao Fang, Anping Zeng

引用次数: 0

Development of Cell Culture Media for Cultivated Meat Production. 养殖肉用细胞培养基的研制。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-01-01 DOI: 10.1007/10_2025_312

M J Post

{"title":"Development of Cell Culture Media for Cultivated Meat Production.","authors":"M J Post","doi":"10.1007/10_2025_312","DOIUrl":"10.1007/10_2025_312","url":null,"abstract":"The culture medium is a central part of the cultivated meat technology, from biological, economical, and safety perspectives. Many cues to drive proliferation or differentiation of cells relevant for cultivated meat are biochemical and are therefore part of the medium. Traditionally, these cues came from fetal bovine serum (FBS), but that has been replaced by a set of components, often of recombinant origin. The culture medium is the costliest input in cultivated meat and therefore subject to intense efforts to reduce cost through simplification, by downgrading components, by replacing components with cheaper alternatives, and by being cognizant of appropriate timing of administration. In addition, reducing the volume of medium per kg of output needed is a subject of research and development. These modifications are all bounded by the need for culture medium to be food-safe and preferably chemically defined. Importantly, the use of medium needs to be as efficient as possible through smart feeding and judicious medium regimes to reduce waste to a minimum. Metabolic models and AI tools will further facilitate medium development leading to less experimental waste. It is expected that this progress will continue for many years leading to ever better media at lower cost, enabling commercialization of cultivated meat.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"63-79"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145814771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Starting Point for Biomolecular Crystallisation. 生物分子结晶的起点。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-01-01 DOI: 10.1007/10_2025_302

Janet Newman, Terese M Bergfors

{"title":"The Starting Point for Biomolecular Crystallisation.","authors":"Janet Newman, Terese M Bergfors","doi":"10.1007/10_2025_302","DOIUrl":"10.1007/10_2025_302","url":null,"abstract":"This chapter presents a historical overview and contemporary insights into the crystallisation of biological macromolecules. It looks at what knowledge has been obtained about the crystallisation of small molecules and how it relates to biomolecules. While the principles of supersaturation and nucleation are the same for both, the complexity of biomolecules presents unique challenges to their crystallisation. Before crystallisation experiments begin with the biomolecule, it is advisable to assay the purity, measure the concentration, and choose a suitable solvent formulation. These parameters are not necessarily straightforward to evaluate. Thereafter, the initial crystallisation screening attempts to examine the effects of chemical space, temperature, pH, and kinetics. Because initial screens do not typically produce diffraction-quality crystals, suggestions are offered for how to optimise screening outcomes. In this context, seeding is particularly powerful, on its own, or in synergy with other optimisation techniques. The chapter concludes with the observation that biomolecular crystallisation remains a poorly understood problem. It is proposed that collecting more information about the crystallisation experiments - both the failed and successful ones - may contribute to a better understanding of the process.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"3-29"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety Aspects of Cell Culture-Derived Food for Human Consumption. 人类食用细胞培养食品的安全问题。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-01-01 DOI: 10.1007/10_2026_333

Fernando Rivero-Pino, Diana C Rueda, Chee Seng Hee, Oana N Kubinyecz, Hannah E Lester

引用次数: 0

Producing Advanced Biofuel Butanol Through Acetone-Butanol-Ethanol Fermentation. 丙酮-丁醇-乙醇发酵生产先进生物燃料丁醇。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-01-01 DOI: 10.1007/10_2026_321

Xue Chuang, Liu Ziyu, Cheng Chi

引用次数: 0

Correction to: Theoretical Perspectives for Biomolecular Crystallization Prediction. 修正：生物分子结晶预测的理论展望。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-01-01 DOI: 10.1007/10_2026_329

James F Lutsko

引用次数: 0

Perspectives Towards AI and ML. 对AI和ML的看法。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-01-01 DOI: 10.1007/10_2025_298

Guoquan Wu, Zhe Wu

{"title":"Perspectives Towards AI and ML.","authors":"Guoquan Wu, Zhe Wu","doi":"10.1007/10_2025_298","DOIUrl":"10.1007/10_2025_298","url":null,"abstract":"This chapter discusses the applications of artificial intelligence (AI) machine learning (ML) to address the practical challenges in biomolecular crystallization processes. Although AI and ML techniques have been successfully applied in many scientific domains, to the best of our knowledge, there is no research that has yet applied ML to develop predictive models for biomolecular crystallization. Therefore, the key focus of this perspective is to demonstrate how ML approaches can be effectively applied to crystallization processes. While biomolecules and small molecules may have different properties and variables to predict, once sensor data are collected and transformed into a data array, the application of machine learning techniques becomes fundamentally similar from a mathematical point of view. Specifically, we start with a brief introduction to various types of ML methods and provide a review of the applications of AI and ML to crystallization. AI and ML have become increasingly important in improving our understanding of biomolecular crystallization, offering new insights and predictive capabilities that were previously unattainable. Subsequently, we introduce three ML approaches to address practical implementation issues in the context of modeling the crystallization processes. Two examples of crystallization processes are presented to demonstrate the effectiveness of the proposed ML algorithms.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"383-418"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of Agricultural Residues Through Nutrient Enrichment for Animal Farming. 通过动物养殖的养分富集来实现农业残留物的增值。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-01-01 DOI: 10.1007/10_2026_328

Minmin Cai, Zhengzheng Zhao

引用次数: 0