Advances in biochemical engineering/biotechnology最新文献_第10页

Microfluidic Devices as Process Development Tools for Cellular Therapy Manufacturing. 微流体装置作为细胞治疗制造的工艺开发工具。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2021_169

Jorge Aranda Hernandez, Christopher Heuer, Janina Bahnemann, Nicolas Szita

{"title":"Microfluidic Devices as Process Development Tools for Cellular Therapy Manufacturing.","authors":"Jorge Aranda Hernandez, Christopher Heuer, Janina Bahnemann, Nicolas Szita","doi":"10.1007/10_2021_169","DOIUrl":"https://doi.org/10.1007/10_2021_169","url":null,"abstract":"Cellular therapies are creating a paradigm shift in the biomanufacturing industry. Particularly for autologous therapies, small-scale processing methods are better suited than the large-scale approaches that are traditionally employed in the industry. Current small-scale methods for manufacturing personalized cell therapies, however, are labour-intensive and involve a number of 'open events'. To overcome these challenges, new cell manufacturing platforms following a GMP-in-a-box concept have recently come on the market (GMP: Good Manufacturing Practice). These are closed automated systems with built-in pumps for fluid handling and sensors for in-process monitoring. At a much smaller scale, microfluidic devices exhibit many of the same features as current GMP-in-a-box systems. They are closed systems, fluids can be processed and manipulated, and sensors integrated for real-time detection of process variables. Fabricated from polymers, they can be made disposable, i.e. single-use. Furthermore, microfluidics offers exquisite spatiotemporal control over the cellular microenvironment, promising both reproducibility and control of outcomes. In this chapter, we consider the challenges in cell manufacturing, highlight recent advances of microfluidic devices for each of the main process steps, and summarize our findings on the current state of the art. As microfluidic cell culture devices have been reported for both adherent and suspension cell cultures, we report on devices for the key process steps, or unit operations, of both stem cell therapies and cell-based immunotherapies.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"179 ","pages":"101-127"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39325513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Droplet Microfluidics for Microbial Biotechnology. 微生物生物技术的微滴流体。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2020_140

Sundar Hengoju, Miguel Tovar, DeDe Kwun Wai Man, Stefanie Buchheim, Miriam A Rosenbaum

{"title":"Droplet Microfluidics for Microbial Biotechnology.","authors":"Sundar Hengoju, Miguel Tovar, DeDe Kwun Wai Man, Stefanie Buchheim, Miriam A Rosenbaum","doi":"10.1007/10_2020_140","DOIUrl":"https://doi.org/10.1007/10_2020_140","url":null,"abstract":"Droplet microfluidics has recently evolved as a prominent platform for high-throughput experimentation for various research fields including microbiology. Key features of droplet microfluidics, like compartmentalization, miniaturization, and parallelization, have enabled many possibilities for microbiology including cultivation of microorganisms at a single-cell level, study of microbial interactions in a community, detection and analysis of microbial products, and screening of extensive microbial libraries with ultrahigh-throughput and minimal reagent consumptions. In this book chapter, we present several aspects and applications of droplet microfluidics for its implementation in various fields of microbial biotechnology. Recent advances in the cultivation of microorganisms in droplets including methods for isolation and domestication of rare microbes are reviewed. Similarly, a comparison of different detection and analysis techniques for microbial activities is summarized. Finally, several microbial applications are discussed with a focus on exploring new antimicrobials and high-throughput enzyme activity screening. We aim to highlight the advantages, limitations, and current developments in droplet microfluidics for microbial biotechnology while envisioning its enormous potential applications in the future.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"179 ","pages":"129-157"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/10_2020_140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38346368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Bioconversion of Methanol by Synthetic Methylotrophy. 合成甲基化甲醇的生物转化。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2021_176

Feng Guo, Shangjie Zhang, Yujia Jiang, Huixin Xu, Fengxue Xin, Wenming Zhang, Min Jiang

引用次数: 1

Flexible Digitization of Highly Individualized Workflows Demonstrated Through the Quality Control of Patient-Specific Cytostatic Application Bags: Digitization from the Perspective of Small and Medium-Sized Laboratories. 通过患者特异性细胞抑制剂应用袋的质量控制展示高度个性化工作流程的灵活数字化:从中小型实验室的角度来看数字化。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2021_190

Max Jochums, Lars M H Reinders, Jochen Tuerk, Thorsten Teutenberg

{"title":"Flexible Digitization of Highly Individualized Workflows Demonstrated Through the Quality Control of Patient-Specific Cytostatic Application Bags: Digitization from the Perspective of Small and Medium-Sized Laboratories.","authors":"Max Jochums, Lars M H Reinders, Jochen Tuerk, Thorsten Teutenberg","doi":"10.1007/10_2021_190","DOIUrl":"https://doi.org/10.1007/10_2021_190","url":null,"abstract":"In order to ensure a high level of product quality and safety, regular quality controls are mandatory, especially in the pharmaceutical industry. These quality controls are strictly regulated and require a high level of documentation. With the goal of complete traceability, these regulations are constantly being tightened, while a majority of laboratories are working still completely paper-based. This leads to an ever-increasing workload that keeps laboratory staff away from value-adding analytical work. In order to realize complete traceability, a reduction in documentation errors and at the same time a reduction of the individual workload, the digitization of complete workflows seems to be a promising solution.Due to the ongoing shortage of IT specialists and the resulting high implementation costs, many laboratories are understandably hesitant. In this chapter an alternative is presented on how to approach the digitization of complete workflows without the need for IT specialists. The example of quality control analysis of cytotoxic drug solutions was chosen to demonstrate the challenges of such a digitization project. In this way, we contribute to a comprehensive understanding of the tools already available, which can also help other laboratories in their digitization efforts. At the end compliance with GMP and EN ISO/IEC 17025 (2017) regulations was reached.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"182 ","pages":"115-129"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39869038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microfluidics for Environmental Applications. 微流体在环境中的应用。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2020_128

Ting Wang, Cecilia Yu, Xing Xie

引用次数: 13

Aerobic Utilization of Methanol for Microbial Growth and Production. 甲醇在微生物生长和生产中的好氧利用。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2021_177

Volker F Wendisch, Gregor Kosec, Stéphanie Heux, Trygve Brautaset

{"title":"Aerobic Utilization of Methanol for Microbial Growth and Production.","authors":"Volker F Wendisch, Gregor Kosec, Stéphanie Heux, Trygve Brautaset","doi":"10.1007/10_2021_177","DOIUrl":"https://doi.org/10.1007/10_2021_177","url":null,"abstract":"Methanol is a reduced one-carbon (C1) compound. It supports growth of aerobic methylotrophs that gain ATP from reduced redox equivalents by respiratory phosphorylation in their electron transport chains. Notably, linear oxidation of methanol to carbon dioxide may yield three reduced redox equivalents if methanol oxidation is NAD-dependent as, e.g., in Bacillus methanolicus. Methanol has a higher degree of reduction per carbon than glucose (6 vs. 4), and thus, lends itself as an ideal carbon source for microbial production of reduced target compounds. However, C-C bond formation in the RuMP or serine cycle, a prerequisite for production of larger molecules, requires ATP and/or reduced redox equivalents. Moreover, heat dissipation and a high demand for oxygen during catabolic oxidation of methanol may pose challenges for fermentation processes. In this chapter, we summarize metabolic pathways for aerobic methanol utilization, aerobic methylotrophs as industrial production hosts, strain engineering, and methanol bioreactor processes. In addition, we provide technological and market outlooks.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"180 ","pages":"169-212"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39610843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Primer on Microfluidics: From Basic Principles to Microfabrication. 微流体入门:从基本原理到微加工。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2020_156

Ann-Kathrin Klein, Andreas Dietzel

引用次数: 0

Emerging Biosensor Trends in Organ-on-a-Chip. 芯片上器官生物传感器的发展趋势。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2020_129

Mario Rothbauer, Peter Ertl

引用次数: 12

Microfluidics in Biotechnology: Quo Vadis. 微流体技术在生物技术中的应用。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/10_2020_162

Steffen Winkler, Alexander Grünberger, Janina Bahnemann

引用次数: 3

Microfluidics in Biotechnology 生物技术中的微流体

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2022-01-01 DOI: 10.1007/978-3-031-04188-4

引用次数: 0