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Advances in biochemical engineering/biotechnology最新文献

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Cell-Free Synthesis and Electrophysiological Analysis of Multipass Voltage-Gated Ion Channels Tethered in Microsomal Membranes. 束缚在微粒体膜中的多通道电压门控离子通道的无细胞合成和电生理分析。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2023_228
Yogesh Pandey, Srujan Kumar Dondapati, Doreen Wüstenhagen, Stefan Kubick
{"title":"Cell-Free Synthesis and Electrophysiological Analysis of Multipass Voltage-Gated Ion Channels Tethered in Microsomal Membranes.","authors":"Yogesh Pandey,&nbsp;Srujan Kumar Dondapati,&nbsp;Doreen Wüstenhagen,&nbsp;Stefan Kubick","doi":"10.1007/10_2023_228","DOIUrl":"10.1007/10_2023_228","url":null,"abstract":"<p><p>Cell-free protein synthesis (CFPS) has emerged as a powerful tool for the rapid synthesis and analysis of various structurally and functionally distinct proteins. These include 'difficult-to-express' membrane proteins such as large multipass ion channel receptors. Owing to their membrane localization, eukaryotic CFPS supplemented with endoplasmic reticulum (ER)-derived microsomal vesicles has proven to be an efficient system for the synthesis of functional membrane proteins. Here we demonstrate the applicability of the eukaryotic cell-free systems based on lysates from the mammalian Chinese Hamster Ovary (CHO) and insect Spodoptera frugiperda (Sf21) cells. We demonstrate the efficiency of the systems in the de novo cell-free synthesis of the human cardiac ion channels: ether-a-go-go potassium channel (hERG) K<sub>V</sub>11.1 and the voltage-gated sodium channel hNa<sub>V</sub>1.5.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"103-120"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10484377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid and Finely-Tuned Expression for Deployable Sensing Applications. 可部署传感应用的快速微调表达式。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2023_223
Alexandra T Patterson, Mark P Styczynski
{"title":"Rapid and Finely-Tuned Expression for Deployable Sensing Applications.","authors":"Alexandra T Patterson,&nbsp;Mark P Styczynski","doi":"10.1007/10_2023_223","DOIUrl":"10.1007/10_2023_223","url":null,"abstract":"<p><p>Organisms from across the tree of life have evolved highly efficient mechanisms for sensing molecules of interest using biomolecular machinery that can in turn be quite valuable for the development of biosensors. However, purification of such machinery for use in in vitro biosensors is costly, while the use of whole cells as in vivo biosensors often leads to long sensor response times and unacceptable sensitivity to the chemical makeup of the sample. Cell-free expression systems overcome these weaknesses by removing the requirements associated with maintaining living sensor cells, allowing for increased function in toxic environments and rapid sensor readout at a production cost that is often more reasonable than purification. Here, we focus on the challenge of implementing cell-free protein expression systems that meet the stringent criteria required for them to serve as the basis for field-deployable biosensors. Fine-tuning expression to meet these requirements can be achieved through careful selection of the sensing and output elements, as well as through optimization of reaction conditions via tuning of DNA/RNA concentrations, lysate preparation methods, and buffer conditions. Through careful sensor engineering, cell-free systems can continue to be successfully used for the production of tightly regulated, rapidly expressing genetic circuits for biosensors.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"141-161"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9632310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Barriers to the Use of Medicinal Mushrooms for Production of Metabolites : The Bjarmin Rushton Story. 使用药用蘑菇生产代谢产物的障碍：Bjarmin-Rushton的故事。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2021_188
Bjørn Kristiansen, M Berovic
{"title":"Barriers to the Use of Medicinal Mushrooms for Production of Metabolites : The Bjarmin Rushton Story.","authors":"Bjørn Kristiansen,&nbsp;M Berovic","doi":"10.1007/10_2021_188","DOIUrl":"10.1007/10_2021_188","url":null,"abstract":"<p><p>Traditionalists are reluctant to leave the technology they and their forefathers knew. To them the new technology based on the stirred tank bioreactor is too removed from the soil the mushroom comes from. On the other hand, there are examples of applications of a bioreactor which support the change from the old to the new technology. In this chapter Bjarmin Rushton, the creator of the well-known medicinal mushroom company Wellness, gives his view of the much talked about difficulties with cultivation in stirred tanks. These problems, are they real or figment of our imagination? Those who read will find out.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"184 ","pages":"107-123"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10641342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SiLA 2: The Next Generation Lab Automation Standard. SiLA 2：下一代实验室自动化标准。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-06-01 DOI: 10.1007/10_2022_204
Daniel Juchli
{"title":"SiLA 2: The Next Generation Lab Automation Standard.","authors":"Daniel Juchli","doi":"10.1007/10_2022_204","DOIUrl":"https://doi.org/10.1007/10_2022_204","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45033675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Overview on Glycosylated Lipids Produced by Bacteria and Fungi: Rhamno-, Sophoro-, Mannosylerythritol and Cellobiose Lipids. 细菌和真菌产生的糖基化脂类综述:鼠李糖、苦参糖、甘露糖赤藓糖醇和纤维素糖脂类。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-05-09 DOI: 10.1007/10_2021_200
S. Zibek, G. Soberón-Chávez
{"title":"Overview on Glycosylated Lipids Produced by Bacteria and Fungi: Rhamno-, Sophoro-, Mannosylerythritol and Cellobiose Lipids.","authors":"S. Zibek, G. Soberón-Chávez","doi":"10.1007/10_2021_200","DOIUrl":"https://doi.org/10.1007/10_2021_200","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41883831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Comparison of Laboratory Standards. 实验室标准比较。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-04-24 DOI: 10.1007/10_2022_205
Matthias Freundel
{"title":"Comparison of Laboratory Standards.","authors":"Matthias Freundel","doi":"10.1007/10_2022_205","DOIUrl":"https://doi.org/10.1007/10_2022_205","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48978988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systems Biology on Acetogenic Bacteria for Utilizing C1 Feedstocks. 产丙酮菌利用C1原料的系统生物学研究。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-04-10 DOI: 10.1007/10_2021_199
Yoseb Song, Jiyun Bae, Jongoh Shin, Sangrak Jin, Seulgi Kang, H. Lee, Suhyung Cho, Byung-Kwan Cho
{"title":"Systems Biology on Acetogenic Bacteria for Utilizing C1 Feedstocks.","authors":"Yoseb Song, Jiyun Bae, Jongoh Shin, Sangrak Jin, Seulgi Kang, H. Lee, Suhyung Cho, Byung-Kwan Cho","doi":"10.1007/10_2021_199","DOIUrl":"https://doi.org/10.1007/10_2021_199","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47331238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation. 工程还原甘氨酸途径:c1同化的一种有前途的合成代谢途径。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-04-02 DOI: 10.1007/10_2021_181
Nico J. Claassens, Ari Satanowski, Viswanada R Bysani, Beau Dronsella, E. Orsi, Vittorio Rainaldi, S. Yilmaz, Sebastian Wenk, Steffen N. Lindner
{"title":"Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation.","authors":"Nico J. Claassens, Ari Satanowski, Viswanada R Bysani, Beau Dronsella, E. Orsi, Vittorio Rainaldi, S. Yilmaz, Sebastian Wenk, Steffen N. Lindner","doi":"10.1007/10_2021_181","DOIUrl":"https://doi.org/10.1007/10_2021_181","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42766243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Biosynthesis Based on One-Carbon Mixotrophy. 基于单碳混合营养的生物合成。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-03-18 DOI: 10.1007/10_2021_198
Yaeseong Hong, A. Zeng
{"title":"Biosynthesis Based on One-Carbon Mixotrophy.","authors":"Yaeseong Hong, A. Zeng","doi":"10.1007/10_2021_198","DOIUrl":"https://doi.org/10.1007/10_2021_198","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45382807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Industrial Perspectives for (Microbial) Biosurfactants. （微生物）生物表面活性剂的工业前景。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-03-18 DOI: 10.1007/10_2021_175
S. Roelants, W. Soetaert
{"title":"Industrial Perspectives for (Microbial) Biosurfactants.","authors":"S. Roelants, W. Soetaert","doi":"10.1007/10_2021_175","DOIUrl":"https://doi.org/10.1007/10_2021_175","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42910415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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