{"title":"Environmental Sustainability of Cultivated Meat.","authors":"Hanna L Tuomisto, Toni Ryynänen","doi":"10.1007/10_2025_293","DOIUrl":"https://doi.org/10.1007/10_2025_293","url":null,"abstract":"<p><p>Cultivated meat is developed as an alternative to livestock meat in reducing the negative externalities of the animal-based food systems. Prospective life cycle assessment studies have been used to estimate the potential environmental impacts of cultivated meat production. Results indicate that cultivated meat production has relatively high industrial energy demand, but lower land use requirements compared to livestock meat production. The climate impacts of cultivated meat depend on the source of energy used. Due to the low land use requirements, cultivated meat could have benefits to biodiversity by reducing the pressure to convert forests and natural habitats to agricultural land. Cultivated meat production could also reduce water use and emissions to waterbodies. The environmental impacts of different cultivated meat production processes are wide depending on the process design and sources of inputs. Life cycle assessment studies can guide the development of cultivated meat production processes towards sustainable options.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"From Muscle Fibers to Functional Foods: Bridging Conventional and Cultivated Approaches.","authors":"Martin Krøyer Rasmussen, Rebekka Rinder Thøgersen, Jette Feveile Young, Margrethe Therkildsen","doi":"10.1007/10_2025_290","DOIUrl":"https://doi.org/10.1007/10_2025_290","url":null,"abstract":"<p><p>This chapter explores the quality attributes of cultivated meat, drawing parallels and distinctions with conventional meat to support future product development. It examines key technical, sensory, and nutritional parameters, like texture, water-holding capacity, color, flavor, and nutritional composition. The chapter highlights how cultivated meat's quality is shaped by cellular composition, scaffold materials, and postharvest processes and how these factors influence consumer-relevant traits such as tenderness, juiciness, and taste. It also discusses the potential of cultivated meat to match or surpass conventional meat in nutritional value, including protein content, amino acid profile, and micronutrient composition. Contributions from five cultivated meat companies provide an industrial context.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Bernfeld, Simon Zschieschang, Marline Kirsch, Jordi Morales-Dalmau, Jasper Liedtke, Antonina Lavrentieva
{"title":"Bioprocess Engineering for Cultivated Meat.","authors":"Julia Bernfeld, Simon Zschieschang, Marline Kirsch, Jordi Morales-Dalmau, Jasper Liedtke, Antonina Lavrentieva","doi":"10.1007/10_2025_292","DOIUrl":"https://doi.org/10.1007/10_2025_292","url":null,"abstract":"<p><p>Cultivated meat (CM) has emerged as a promising solution to the environmental, ethical, and food security concerns associated with conventional meat production. However, realising its full potential depends on developing robust, scalable, and cost-effective bioprocessing strategies. This chapter explores the entire CM bioprocessing pipeline, from upstream challenges such as the development of robust cell lines, scaling up cell cultures, and evaluating different operating modes like batch, fed-batch, and perfusion to downstream processes such as biomass harvesting, purification, and product structuring. Key considerations include ensuring sterility, monitoring critical process parameters, and supporting effective cell proliferation and differentiation. Economically, the high cost of media and capital expenditures remain a major barrier to scale. Strategies such as media recycling, bulk ingredient sourcing, and in-house production are being explored to reduce costs. Emerging technologies like artificial intelligence, machine learning, and digital twins offer new tools for optimising operations, though adoption is still in early stages. As the industry progresses toward commercialisation, continued innovation in bioprocess engineering, guided by techno-economic modelling, will be crucial to achieving scalable and sustainable meat alternatives.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction to: The Human Gut Microbiota: A Dynamic Biologic Factory.","authors":"Alireza Minagar, Rabih Jabbour","doi":"10.1007/10_2024_253","DOIUrl":"10.1007/10_2024_253","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"243"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Human Gut Microbiota: A Dynamic Biologic Factory.","authors":"Alireza Minagar, Rabih Jabbour","doi":"10.1007/10_2023_243","DOIUrl":"10.1007/10_2023_243","url":null,"abstract":"<p><p>The human body constitutes a living environment for trillions of microorganisms, which establish the microbiome and, the largest population among them, reside within the gastrointestinal tract, establishing the gut microbiota. The term \"gut microbiota\" refers to a set of many microorganisms [mainly bacteria], which live symbiotically within the human host. The term \"microbiome\" means the collective genomic content of these microorganisms. The number of bacterial cells within the gut microbiota exceeds the host's cells; collectively and their genes quantitatively surpass the host's genes. Immense scientific research into the nature and function of the gut microbiota is unraveling its roles in certain human health activities such as metabolic, physiology, and immune activities and also in pathologic states and diseases. Interestingly, the microbiota, a dynamic ecosystem, inhabits a particular environment such as the human mouth or gut. Human microbiota can evolve and even adapt to the host's unique features such as eating habits, genetic makeup, underlying diseases, and even personalized habits. In the past decade, biologists and bioinformaticians have concentrated their research effort on the potential roles of the gut microbiome in the development of human diseases, particularly immune-mediated diseases and colorectal cancer, and have initiated the assessment of the impact of the gut microbiome on the host genome. In the present chapter, we focus on the biological features of gut microbiota, its physiology as a biological factory, and its impacts on the host's health and disease status.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"91-106"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139711174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tochukwu Ekwonna, Olusegun Akindeju, Brianna Amos, Zhi-Qing Lin
{"title":"Selenium Removal from Wastewater by Microbial Transformation and Volatilization.","authors":"Tochukwu Ekwonna, Olusegun Akindeju, Brianna Amos, Zhi-Qing Lin","doi":"10.1007/10_2023_242","DOIUrl":"10.1007/10_2023_242","url":null,"abstract":"<p><p>Selenium (Se) is a naturally occurring trace element that is nutritionally essential for humans and animals, but becomes toxic at high concentrations. This laboratory study explored the role of microbes in Se removal from contaminated wastewater via biological transformation and volatilization processes. Microbes could immobilize water-soluble selenate (SeO<sub>4</sub><sup>2-</sup>) and selenite (SeO<sub>3</sub><sup>2-</sup>) to water-insoluble elemental Se (Se<sup>0</sup>) and transform Se into volatile Se compounds found in the atmosphere. Results of this laboratory study showed that Bacillus cereus, a bacterial strain isolated from wheat straw and biosolid-WTR-sand substrates showed a significant biotransformation ability of reducing selenate and selenite to elemental Se and forming volatile Se organic compounds in wastewater. Overall, microbial Se chemical reduction, methylation, and volatilization are important processes in bioremediation of Se-contaminated wastewater.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"125-136"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139711172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manisha Khedkar, Dattatray Bedade, Rekha S Singhal, Sandip B Bankar
{"title":"Mixed Culture Cultivation in Microbial Bioprocesses.","authors":"Manisha Khedkar, Dattatray Bedade, Rekha S Singhal, Sandip B Bankar","doi":"10.1007/10_2023_248","DOIUrl":"10.1007/10_2023_248","url":null,"abstract":"<p><p>Mixed culture cultivation is well renowned for industrial applications due to its technological and economic benefits in bioprocess, food processing, and pharmaceutical industries. A mixed consortium encompasses to achieve growth in unsterile conditions, robustness to environmental stresses, perform difficult functions, show better substrate utilization, and increase productivity. Hence, mixed cultures are being valorized currently and has also augmented our understanding of microbial activities in communities. This chapter covers a wide range of discussion on recent improvements in mixed culture cultivation for microbial bioprocessing and multifarious applications in different areas. The history of microbial culture, microbial metabolism in mixed culture, biosynthetic pathway studies, isolation and identification of strains, along with the types of microbial interactions involved during their production and propagation, are meticulously detailed in the current chapter. Besides, parameters for evaluating mixed culture performance, large-scale production, and challenges associated with it are also discussed vividly. Microbial community, characteristics of single and mixed culture fermentation, and microbe-microbe interactions in mixed cultures have been summarized comprehensively. Lastly, various challenges and opportunities in the area of microbial mixed culture that are obligatory to improve the current knowledge of microbial bioprocesses are projected.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"9-69"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erik Dahlquist, Eva Thorin, Aubrey Shenk, Sebastian Schwede, Chaudhary Awais Salman, Elias Hakalehto
{"title":"Investigation of Upgrading of Products from Finnoflag Bio-refinery Pilot in Tampere.","authors":"Erik Dahlquist, Eva Thorin, Aubrey Shenk, Sebastian Schwede, Chaudhary Awais Salman, Elias Hakalehto","doi":"10.1007/10_2024_261","DOIUrl":"10.1007/10_2024_261","url":null,"abstract":"<p><p>In this study calculation over material and energy balances for bio-refinery product upgrading using membrane filtration (MF, UF, and RO), distillation, and ion-exchanger has been performed. Tests have been made with UF filtration in a pilot plant, separation tests made at lab with ion-exchanger and simulation using ASPEN plus simulator for distillation. Rough economic analysis has been made for the different solutions/techniques.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"213-240"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Runze Pan, Jingxiang Sun, Fengxue Xin, Wankui Jiang, Min Jiang
{"title":"Enzymes for Biomass Pretreatment: A Comprehensive Review.","authors":"Runze Pan, Jingxiang Sun, Fengxue Xin, Wankui Jiang, Min Jiang","doi":"10.1007/10_2025_275","DOIUrl":"10.1007/10_2025_275","url":null,"abstract":"<p><p>Biomass pretreatment plays a crucial role in the conversion of lignocellulosic biowaste materials into valuable biofuels and biochemicals. Enzymatic pretreatment, in particular, has gained significant attention due to its eco-friendly nature and efficiency in breaking down complex biomass structures. This comprehensive review aims to provide an overview of enzymes used in biomass pretreatment, including cellulases, hemicellulases, ligninases, and their applications in enhancing the efficiency of biomass conversion processes. The review also discusses recent advancements, challenges, and future prospects in the field of enzymatic biomass pretreatment.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"99-117"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Niël van Wyk, Claudia Borgmeier, Alice Kleber, Esther M Gabor
{"title":"Sustainable Approaches in Viticulture: From Wastes and Side Streams to High-Value Products.","authors":"Niël van Wyk, Claudia Borgmeier, Alice Kleber, Esther M Gabor","doi":"10.1007/10_2025_281","DOIUrl":"10.1007/10_2025_281","url":null,"abstract":"<p><p>In the context of climate change, resource scarcity, and a growing global population, sustainable processes and the efficient use of raw materials are imperative. Developing best practices to guide the transition toward a circular economy with minimized waste is essential. Agricultural processes offer inspiration, as they traditionally emphasize the utilization of all parts of cultivated plants. Today, however, there is a pressing need to go beyond basic utilization and focus on the upcycling and valorization of agricultural side streams into high-value products. This chapter examines the wine and grape industry, which generates substantial volumes of side streams, including grape pomace, seeds, stems, and wine lees. In recent years, these material streams have attracted attention for their potential as raw materials rich in bioactive compounds - such as diverse polyphenols, dietary fibers, and organic acids - that can be applied across various sectors, including food, cosmetics, pharmaceuticals, and bioenergy. Key methodologies such as extraction, fermentation, and bioconversion are discussed as pathways to recover and enhance these compounds. Case studies of successful valorization initiatives are presented, demonstrating practical applications of side streams for high-value product creation. An example is the BMBF-funded project \"Sustainable Beverages\" focusing on the development of fermented drinks made from vine leaves that can be offered as a non-alcoholic alternative to wine. By providing an overview of the current research and technologies in wine waste valorization, this chapter serves as a valuable resource for academics, industry professionals, and policymakers seeking to advance sustainable practices within the wine sector. Ultimately, the upcycling of wine industry residues not only reduces environmental impact but also creates new revenue opportunities, driving innovation and sustainability in the industry.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"151-184"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}