Advances in biochemical engineering/biotechnology最新文献

{"title":"Biomanufacturing of Succinic Acid.","authors":"Yongyan Xi, Xinna Zhu, Hongtao Xu, Jinlei Tang, Tao Zhan, Feiyu Fan, Xueli Zhang","doi":"10.1007/10_2026_343","DOIUrl":"https://doi.org/10.1007/10_2026_343","url":null,"abstract":"<p><p>Succinic acid (SA) is a key platform chemical with broad applications in biodegradable materials, pharmaceuticals, and food additives, and its market demand continues to expand rapidly. Conventional production processes for SA rely heavily on fossil resources, are associated with substantial carbon emissions, and impose significant environmental burdens, rendering them incompatible with global carbon neutrality goals and the ongoing transition toward sustainable, low-carbon development. Microbial biosynthesis, by contrast, utilizes renewable carbon feedstocks under mild operating conditions and exhibits high atom economy, offering a promising alternative to petrochemical routes. In this review, we systematically trace the historical development of bio-based SA, focusing on technological innovations aimed at cost reduction. We summarize the principal metabolic pathways involved in SA biosynthesis, and critically examine advances in native SA-producing rumen bacteria, metabolically engineered bacteria, and yeast chassis systems. Furthermore, we objectively analyze the application of non-food feedstocks in bio-based SA production and highlight that, despite the current lack of economic competitiveness, such feedstocks represent a crucial direction for the future green and sustainable development of bio-based SA. Finally, we analyze the commercialization trajectory and strategic landscape of bio-based SA both globally and in China. This review aims to provide a conceptual framework and theoretical insights to facilitate the transition of green SA biomanufacturing from laboratory research to large-scale industrial implementation.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Review of Enzyme and Nanozyme Biosensors: Design Methodology, Design Tool, and Prospects.","authors":"Dandan Zhai, Shilong Wang, Ying Li, Qianhui Yuan, Pengyang Dong, Anqi Liu","doi":"10.1007/10_2026_347","DOIUrl":"https://doi.org/10.1007/10_2026_347","url":null,"abstract":"<p><p>Natural enzymes and nanozymes represent complementary paradigms for catalytic biosensing: the former provides evolutionarily refined molecular recognition and exceptional catalytic efficiency, while the latter offers tunable, robust, and manufacturable nanomaterial-based catalysis. Recent advances span catalytic theory, design and fabrication, and application-driven integration - illustrating how immobilization chemistries, protein engineering, and nanostructured transducer interfaces can preserve or recapitulate active-site function, and how defect engineering, single-atom centers, and two-dimensional materials deliver enhanced activity and environmental resilience. Persistent technical bottlenecks include enzyme fragility and cold-chain dependence, as well as nanozyme issues of biocompatibility, substrate selectivity, and batch reproducibility. These limitations are being addressed through convergent innovations: microfluidic platforms and scalable synthesis routes improve sampling and manufacturability, while machine-learning-assisted design and advanced signal-processing restore effective selectivity and enable rapid candidate triage. Emphasis on integrated toolchains - curated databases, multiscale simulation, and AI models - facilitates cross-domain knowledge transfer between protein and materials design. Finally, translational readiness will depend on standardized metrics, regulatory validation, and interdisciplinary consortia; near-term impact is expected in targeted applications (clinical biomarkers, antibiotic residues, environmental pollutants) where hybrid enzyme-nanozyme systems and data-driven workflows can deliver field-ready, robust sensors.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-Generation Industrial Biotechnology (NGIB) for Sustainable Biomanufacturing.","authors":"Shuang Zheng, Guo-Qiang Chen","doi":"10.1007/10_2026_364","DOIUrl":"https://doi.org/10.1007/10_2026_364","url":null,"abstract":"<p><p>Industrial biotechnology is focused on the bioprocessing of sustainable and cost-effective substrates, such as starch sugar, lignocellulose, and C1 feedstocks, to produce high-value compounds and bulk chemicals. However, traditional bioprocesses necessitate the use of stainless steel bioreactors, intricate sterilization protocols, burdensome and expensive posttreatment procedures, as well as skilled engineers to execute bioprocessing under aseptic conditions. These factors undermine the competitiveness of bioproducts. In contrast, next-generation industrial biotechnology (NGIB) harnesses extreme microorganisms, including halophiles, thermophiles, psychrophiles, acidophiles, and alkaliphiles, as its chassis. These microorganisms can proliferate rapidly in extreme environments while effectively resisting contamination from other microorganisms. NGIB facilitates continuous fermentations in open non-sterile environments using bioreactors crafted from ceramic, cement, or plastic materials. This approach offers numerous advantages, including energy and freshwater savings, material conversion efficiency, and streamlined operational procedures. The advancement of NGIB holds tremendous potential for sustainable biosynthesis of bioproducts.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and Fate of PFAS During Conventional and Advanced Wastewater Treatment.","authors":"Rodoshi Ahmed, Benedikt M Aumeier, Jörg E Drewes","doi":"10.1007/10_2026_361","DOIUrl":"https://doi.org/10.1007/10_2026_361","url":null,"abstract":"<p><p>This chapter is dedicated to the fate of per- and polyfluorinated alkyl substances during wastewater treatment, also known as \"forever chemicals\" due to their very high persistence in the environment. First, their exceptional physicochemical properties are examined to derive their value for industrial applications, their adverse effects on human and environmental health, and starting points for technologies to remove or eliminate them from water. Second, the sources and occurrences of PFAS in the aquatic environment are also presented summarizing the regulatory framework within the European Union. Third, removal technologies are presented and discussed including conventional wastewater treatment as well as established and emerging advanced technologies to remove PFAS from wastewater effluents. At the end, a summary and outlook are provided.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Approaches in Protein Structure/Function Analysis, Design, and Engineering.","authors":"Zhoutong Sun, Bo Yuan, Jinghao Shi, Yikai Wang","doi":"10.1007/10_2026_369","DOIUrl":"https://doi.org/10.1007/10_2026_369","url":null,"abstract":"<p><p>Over the past decade, protein design has drawn considerable attention and emerged as a promising tool for sustainable and efficient biocatalytic processes, driven by breakthroughs in DNA sequencing, gene synthesis, bioinformatics, and artificial intelligence. Enabled by strategies such as directed evolution, structure/function analysis, and (semi-)rational design, engineered enzymes can now accommodate nonnatural substrates, catalyze novel reactions, and operate under harsh conditions including elevated temperatures and organic solvents.This chapter provides a comprehensive overview of major strategies in protein design including directed evolution, rational design, and semi-rational design. Emphasis is placed on recent advances in high-throughput screening technologies, identification of functionally critical residues, and optimization of site-specific mutations. Furthermore, the integration of artificial intelligence and multiscale modeling to enzyme design is discussed, highlighting data-driven decision-making and deep learning-assisted prediction of enzymatic performance. Looking forward, biocatalysis is poised to further expand its applications in valorization of renewable resources, biofuels, and synthetic chemistry. Powered by ongoing advances in AI and computational protein design, the development of enzymes with unprecedented catalytic functions is anticipated to accelerate rapidly.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mainstream Nitrogen Removal in Wastewater Treatment: Nitritation, Anammox, and Partial Denitrification.","authors":"Alba Pedrouso, Cristopher da Silva, Anuska Mosquera-Corral, J Luis Campos","doi":"10.1007/10_2026_359","DOIUrl":"https://doi.org/10.1007/10_2026_359","url":null,"abstract":"<p><p>The revised EU Urban Wastewater Treatment Directive (UWWTD) EU 2024/3019 imposes stricter nitrogen discharge limits (8-10 mg N/L) and mandates energy neutrality by 2045 for WWTPs >10,000 population equivalents, challenging conventional biological nutrient removal processes. Mainstream partial nitritation and anammox (PN/A) processes for autotrophic nitrogen removal are a good alternative for achieving energy-neutral wastewater treatment. However, maintaining stability under fluctuating mainstream conditions, along with low water temperatures (<20°C) and low nitrogen concentrations (<100 mg N/L), remains difficult. This chapter evaluates the current state of mainstream PN/A processes, focusing on pilot-scale developments and bottlenecks associated with suppressing nitrite-oxidizing bacteria (NOB) activity. By analyzing the ecological niches of microbial guilds, this analysis explores why NOB, such as Nitrospira, persist under mainstream conditions and how metabolic stressors can be leveraged to select for them. Moreover, the possible replacement of NOB species is discussed, highlighting the difficulty of their absolute suppression. Given the challenges of supplying nitrite to anammox via nitritation, alternative metabolic pathways, including partial heterotrophic denitrification and sulfur-driven processes, are discussed. While stoichiometry dictates that, in PN/A processes, approximately 11% of the converted nitrogen remains as nitrate, coupling anammox with complementary processes provides a resilient, resource-efficient approach. Ultimately, the future of nitrogen removal depends on flexible, interconnected treatment schemes that enable regulatory compliance.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomanufacturing of myo-Inositol and Healthy Sugars via In Vitro Biotransformation (ivBT).","authors":"Zhenyu Zhai, Yi-Heng P Job Zhang","doi":"10.1007/10_2026_358","DOIUrl":"https://doi.org/10.1007/10_2026_358","url":null,"abstract":"<p><p>Biomanufacturing holds immense promise for the sustainable development of human society. Over the past decade, in vitro biotransformation (ivBT) has emerged as a cost-competitive platform for the biomanufacturing of diverse biocommodities. The industrialization of ivBT began with myo-inositol, followed closely by the upcoming commercialization of two healthy sugars (i.e., D-tagatose and D-mannose). Currently, ivBT enables the world's largest myo-inositol factory owned by Bohaoda Biotechnology Co. Ltd. located in Sichuan, China, with an annual production capacity of 10,000 tons, while the industrial-scale facilities for D-tagatose and D-mannose are under development in Tianjin, China. In this chapter, we introduce the biomanufacturing of myo-inositol and a healthy sugar (i.e., D-tagatose) via ivBT. We highlight the importance of these products, their production methods, and the unique advantages of ivBT. Notably, we provide a first-hand account on the R&D journey behind these innovations. Overall, ivBT was expected to open the era of Biomanufacturing 4.0, contributing to the industrial biomanufacturing of not only inositol and healthy sugars, but also artificial food and renewable energy.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Bioproduction of Feed Amino Acids in China.","authors":"Liu Jingui, Wang Lintao, Han Xuan, Shi Xinlei, Chen Liang, Wu Heyun, Ma Qian, Xie Xixian","doi":"10.1007/10_2026_337","DOIUrl":"https://doi.org/10.1007/10_2026_337","url":null,"abstract":"<p><p>Proteins are one of the essential macromolecules that constitute the foundation of life. Amino acids are the building blocks of proteins. Since the early production of monosodium glutamate (MSG), many amino acids have been widely produced for applications in pharmaceuticals, feed additives, and animal nutrition. In livestock and poultry industries, feed amino acids help reduce crude protein content and balance animal nutrition. Amid global food security concerns, demand is growing for amino acids to lessen the reliance on soybean meal in feed. Thanks to their higher quality and lower cost relative to chemical methods, most amino acids are now produced via microbial fermentation. Among these, L-lysine, L-methionine, and L-threonine account for the majority of global consumption, representing approximately 80-90% of the total feed amino acid market. Other amino acids such as L-tryptophan, L-isoleucine, L-valine, L-histidine, and L-arginine command a small market share due to their limited production capacity. Therefore, improving the efficiency of producing amino acids for animal feed is crucial. This is not only about securing better feed supply chains but also a major step toward building a more sustainable livestock industry. Synthetic biology uses renewable resources or CO₂ as raw materials, replacing traditional chemical synthesis. Synthetic biology has been continuously improving in areas such as gene editing, metabolic pathway regulation, and AI-assisted design, leading to a continued decline in the production costs of minor amino acids such as valine, tryptophan, isoleucine, arginine, and histidine. This technological advance is expected to drive further expansion in demand for these minor amino acids, thereby pushing the whole feed amino acid market growth even higher.In this chapter, we review the technologies and strategies employed in developing high-yield strains for the five feed minor amino acids (L-tryptophan, L-isoleucine, L-valine, L-histidine, and L-arginine). This section reviews the development of China's feed amino acid industry, covering production strategies, key manufacturers, the country's share of global capacity, and future growth prospects.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Biosensors for Screening and Regulating Hyper-Producing Cell Factory.","authors":"Zhen Fang, Yong Jiang","doi":"10.1007/10_2026_351","DOIUrl":"https://doi.org/10.1007/10_2026_351","url":null,"abstract":"<p><p>Microorganisms have been identified as versatile cell factories for the synthesis of valuable natural products, biofuels, and bulk chemicals. The above biosynthetic processes dependent on intracellular metabolic pathways and enzymes exhibit more advantages (such as sustainable process, high selectivity, and precise modification) than traditional fossil industry. However, it is still challenging to achieve ideal hyper-producing cell factory because of the complicated metabolite concentration measurement, metabolic pathway regulation, and biosynthetic pathway optimization. Fortunately, molecular biosensor can respond to specific metabolites within the cell and convert the molecular recognition into a readable signal that inspires research to artificially control cell metabolism by an elaborate biosensor. In this book chapter, we introduce the basic mechanism and design principles of metabolite-related molecular biosensors. We also summarize the classical application cases of screening and regulating molecular biosensors to create hyper-producing cell factories. Although it poses some challenges, the development of artificial intelligence and synthetic biology approaches will provide more possibilities of using molecular biosensors to achieve ideal yield and productivity in microbial cell factories.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical Biosensor-Based Detection of Drug-Resistant Bacteria Using Lateral Flow Immunoassay.","authors":"Sallahuddin Panhwar, Hareef Ahmed Keerio, Muhammad Usman Akram, Said Nawab, Ugur Tamer","doi":"10.1007/10_2026_349","DOIUrl":"https://doi.org/10.1007/10_2026_349","url":null,"abstract":"<p><p>The worldwide dissemination of antimicrobial-resistant pathogens via the food supply chain now represents a critical challenge for international health. Ensuring the integrity of food products necessitates the development of methodologies for the swift identification of resistant bacterial strains. Optical biosensing platforms utilizing lateral flow immunochromatographic assay (LFIA) principles have garnered substantial interest for their capacity to perform point-of-need detection via portable strips. This discussion evaluates contemporary advancements in optical LFIA technologies, specifically focusing on strategies for enhancing analytical sensitivity, selectivity, and the integration of antibody-modified substrates. Furthermore, this review categorizes specific drug-resistant bacterial targets and explores their detection through various practical and industrial lenses. The concluding segments emphasize the utility of LFIA as a versatile instrument for epidemiological tracking, detailed microbial assessment, and real-time surveillance within food production environments.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}