Advances in biochemical engineering/biotechnology最新文献

Engineered Microbial Strains for Co-fermentation of C5 and C6 Sugars to Produce Cellulosic Ethanol. C5和C6糖共发酵生产纤维素乙醇的工程微生物菌株。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-05-07 DOI: 10.1007/10_2026_319

Ryo Nasuno, Cai-Yun Xie, Yoshiaki Kawahara, Yue-Qin Tang, Tomohisa Hasunuma

{"title":"Engineered Microbial Strains for Co-fermentation of C5 and C6 Sugars to Produce Cellulosic Ethanol.","authors":"Ryo Nasuno, Cai-Yun Xie, Yoshiaki Kawahara, Yue-Qin Tang, Tomohisa Hasunuma","doi":"10.1007/10_2026_319","DOIUrl":"https://doi.org/10.1007/10_2026_319","url":null,"abstract":"Efficient fermentation of both hexose (C6) and pentose (C5) sugars derived from lignocellulosic biomass is essential for the sustainable production of cellulosic ethanol. However, conventional yeast Saccharomyces cerevisiae lacks native pathways for pentose utilization and is susceptible to various environmental stresses caused by hydrolysate-rich fermentation conditions. This chapter outlines the metabolic engineering strategies for introducing and optimizing C5 pathways alongside native C6 metabolism. It highlights advances in recombinant strain development, including synthetic pathway design, cofactor balancing, and transporter engineering. We also review approaches for consolidated bioprocessing that improve overall productivity by combining saccharification and fermentation in a single batch. These approaches include the secretion or cell surface display of cellulases and hemicellulases and optimizing their expression levels. Furthermore, this chapter introduces tolerance mechanisms against stresses derived from fermentation conditions including high temperature, osmolarity, and ethanol, as well as lignocellulose hydrolysate-derived inhibitors such as acetic acid and furfural. By connecting innovations in metabolic design and stress response, the chapter provides a comprehensive view of the current progress and future directions in developing yeast strains to co-ferment C5/C6 sugars under industrially practical conditions.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life Cycle Analysis for Agricultural Residues and Related Products. 农业残留物及其相关产品的生命周期分析。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-05-07 DOI: 10.1007/10_2026_327

Penjit Srinophakun, Anusith Thanapimmetha, Nutchapon Chiarasumran, Khemmathin Lueangwattanapong, Maythee Saisriyoot

{"title":"Life Cycle Analysis for Agricultural Residues and Related Products.","authors":"Penjit Srinophakun, Anusith Thanapimmetha, Nutchapon Chiarasumran, Khemmathin Lueangwattanapong, Maythee Saisriyoot","doi":"10.1007/10_2026_327","DOIUrl":"https://doi.org/10.1007/10_2026_327","url":null,"abstract":"Agricultural residues represent an abundant and underutilized resource that can be redirected from disposal pathways toward value-added applications such as composting, mulching, bioenergy, land amendment, and bioplastics. This chapter examines these pathways through the perspective of life cycle assessment (LCA), highlighting how agricultural residues can contribute to waste reduction, soil improvement, greenhouse gas mitigation, and circular resource use. Across the reviewed case studies, environmental outcomes are shown to depend not only on the residue itself, but also on the selected conversion pathway, management practice, system boundary, and local implementation context. Composting and mulching can reduce environmental burdens, although benefits remain sensitive to process modifications, additive use, and field conditions. Energy recovery from residues can offer substantial gains when carbon-intensive reference systems are displaced, but performance varies with logistics and conversion efficiency. Land application may improve soil quality and carbon retention while also shifting greenhouse gas fluxes depending on amendment type and site conditions. Likewise, residue-derived bioplastics can lower impacts relative to fossil-based plastics under favorable feedstock and end-of-life scenarios, but these benefits are not universal. Overall, the chapter shows that LCA is a valuable tool for comparing residue management options while also emphasizing the need for integrated assessment frameworks that account for environmental, technical, and implementation-related factors when selecting sustainable valorization strategies.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lignocellulosic Biomass Resources for Sustainable Socioeconomic Development. 可持续社会经济发展的木质纤维素生物质资源。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-05-07 DOI: 10.1007/10_2026_316

Pramuk Parakulsuksatid, Alfred Elikem Kwami Afedzi

{"title":"Lignocellulosic Biomass Resources for Sustainable Socioeconomic Development.","authors":"Pramuk Parakulsuksatid, Alfred Elikem Kwami Afedzi","doi":"10.1007/10_2026_316","DOIUrl":"https://doi.org/10.1007/10_2026_316","url":null,"abstract":"Agricultural residues including corn stover, rice straw, sugarcane bagasse, cereal straws, oil palm biomass, and many others are often burned or left to decompose, releasing avoidable emissions and losing nutrients. Redirected to modern biorefineries, they become a renewable carbon feedstock for biofuels, biogas, chemicals, and biomaterials. This chapter quantifies regional residue supplies and finds that Asia, the Americas, Europe, and Africa together generate more than 3 billion dry tons of residues yearly. Life cycle assessments report up to 90% greenhouse gas savings compared with fossil routes, particularly when open-field burning is eliminated. Technical advances in pretreatment, engineered enzymes, co-fermentation, and thermochemical upgrading now enable high-yield multiproduct conversion. Policy instruments such as feed-in tariffs, biogas purchase guarantees, carbon credits, and sustainability certifications lower investment risk and accelerate deployment. Cooperative models for collection, densification, transport, and processing create employment for farmers, women, and rural youth. Upgraded roads, storage, and off-grid energy systems follow. By integrating job creation, income diversification, and infrastructure development with rigorous soil and carbon safeguards, agricultural residues can transform from low-value waste into the foundation of a circular, low-carbon bioeconomy. Future studies should integrate advanced process innovations with equity strategies to secure resilient rural livelihoods.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

External Fields as Control Strategies to Promote Biomolecule Nucleation. 外场作为促进生物分子成核的控制策略。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-04-30 DOI: 10.1007/10_2025_311

Rohan P Y van Tooren, Hüseyin Burak Eral

引用次数: 0

Perspectives Towards AI and ML. 对AI和ML的看法。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-04-17 DOI: 10.1007/10_2025_298

Guoquan Wu, Zhe Wu

{"title":"Perspectives Towards AI and ML.","authors":"Guoquan Wu, Zhe Wu","doi":"10.1007/10_2025_298","DOIUrl":"https://doi.org/10.1007/10_2025_298","url":null,"abstract":"This chapter discusses the applications of artificial intelligence (AI) machine learning (ML) to address the practical challenges in biomolecular crystallization processes. Although AI and ML techniques have been successfully applied in many scientific domains, to the best of our knowledge, there is no research that has yet applied ML to develop predictive models for biomolecular crystallization. Therefore, the key focus of this perspective is to demonstrate how ML approaches can be effectively applied to crystallization processes. While biomolecules and small molecules may have different properties and variables to predict, once sensor data are collected and transformed into a data array, the application of machine learning techniques becomes fundamentally similar from a mathematical point of view. Specifically, we start with a brief introduction to various types of ML methods and provide a review of the applications of AI and ML to crystallization. AI and ML have become increasingly important in improving our understanding of biomolecular crystallization, offering new insights and predictive capabilities that were previously unattainable. Subsequently, we introduce three ML approaches to address practical implementation issues in the context of modeling the crystallization processes. Two examples of crystallization processes are presented to demonstrate the effectiveness of the proposed ML algorithms.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Other Techniques. 其他技术。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-04-07 DOI: 10.1007/10_2026_313

Ana M Gonçalves, Marino F A Santos, Luís A Passarinha

{"title":"Other Techniques.","authors":"Ana M Gonçalves, Marino F A Santos, Luís A Passarinha","doi":"10.1007/10_2026_313","DOIUrl":"https://doi.org/10.1007/10_2026_313","url":null,"abstract":"The production of protein crystals suitable for high-resolution structural analysis critically depends on the quality, homogeneity, and functional integrity of the protein sample throughout the crystallization workflow. This chapter provides a comprehensive overview of key biochemical and biophysical parameters that govern successful protein crystallization, including protein purity, conformational and compositional homogeneity, structural integrity, and biological activity. We discuss the application of different complementary techniques - including chromatographic methods, mass spectrometry, circular dichroism, surface plasmon resonance, and X-ray crystallography - to systematically assess and optimize these parameters from initial sample preparation to final crystal formation and subsequent X-ray data collection. By integrating orthogonal characterization approaches, common issues such as aggregation, degradation, conformational heterogeneity, and loss of function can be identified and mitigated prior to crystallization trials, thereby improving reproducibility and diffraction quality. Furthermore, the chapter highlights alternative X-ray data collection methodologies such as room-temperature, in situ, and automated data collection that further enhances crystallographic workflows by reducing crystal handling, improving experimental efficiency, and enabling the capture of biologically relevant conformational states.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147626923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomolecular Crystallization in Microfluidic Devices. 微流体器件中的生物分子结晶。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-04-07 DOI: 10.1007/10_2025_299

Nadine Candoni, Romain Grossier, Stéphane Veesler

{"title":"Biomolecular Crystallization in Microfluidic Devices.","authors":"Nadine Candoni, Romain Grossier, Stéphane Veesler","doi":"10.1007/10_2025_299","DOIUrl":"https://doi.org/10.1007/10_2025_299","url":null,"abstract":"This chapter presents an overview of microfluidic devices reported in the literature, used to develop methodologies for nucleation of biomolecules, with crystal size control, and for collecting thermodynamic and kinetic data. Section 2 is dedicated to the properties of microfluidic devices through materials used for their fabrication and for crystal analysis. Section 3 describes the variety of microfluidic devices available and how to handle them to produce flows, droplets, and/or wells of micrometer size. These devices use crystallization methods inspired by batch processes, and they are mainly used for protein crystallization. Section 4 focuses on fundamental properties of biomolecule crystallization determined using droplet-based microfluidics: nucleation kinetics, nucleation rate, and effective interfacial energy crystal/solution. Section 5 explains how the kinetic effect of confinement due to micrometer size, and so nanovolumes, leads to isolation of different phases. These latter are characterized by X-ray diffraction (XRD), and methods to minimize manual handling of crystals for XRD are also presented, with appropriate equipment to store the crystals.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147626906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Starting Point for Biomolecular Crystallisation. 生物分子结晶的起点。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-03-31 DOI: 10.1007/10_2025_302

Janet Newman, Terese M Bergfors

{"title":"The Starting Point for Biomolecular Crystallisation.","authors":"Janet Newman, Terese M Bergfors","doi":"10.1007/10_2025_302","DOIUrl":"https://doi.org/10.1007/10_2025_302","url":null,"abstract":"This chapter presents a historical overview and contemporary insights into the crystallisation of biological macromolecules. It looks at what knowledge has been obtained about the crystallisation of small molecules and how it relates to biomolecules. While the principles of supersaturation and nucleation are the same for both, the complexity of biomolecules presents unique challenges to their crystallisation. Before crystallisation experiments begin with the biomolecule, it is advisable to assay the purity, measure the concentration, and choose a suitable solvent formulation. These parameters are not necessarily straightforward to evaluate. Thereafter, the initial crystallisation screening attempts to examine the effects of chemical space, temperature, pH, and kinetics. Because initial screens do not typically produce diffraction-quality crystals, suggestions are offered for how to optimise screening outcomes. In this context, seeding is particularly powerful, on its own, or in synergy with other optimisation techniques. The chapter concludes with the observation that biomolecular crystallisation remains a poorly understood problem. It is proposed that collecting more information about the crystallisation experiments - both the failed and successful ones - may contribute to a better understanding of the process.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ Online Spectroscopy and Light Scattering Techniques. 原位在线光谱学和光散射技术。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-03-31 DOI: 10.1007/10_2026_315

Susanna Gevorgyan, Hévila Brognaro, Christian Betzel

{"title":"In Situ Online Spectroscopy and Light Scattering Techniques.","authors":"Susanna Gevorgyan, Hévila Brognaro, Christian Betzel","doi":"10.1007/10_2026_315","DOIUrl":"https://doi.org/10.1007/10_2026_315","url":null,"abstract":"Within this chapter, we focus on explaining and introducing latest light scattering techniques as dynamic light scattering (DLS) applied to score and optimize biomolecular solutions prior to crystallization experiments. DLS is today the most common and noninvasive method used to obtain information about the dimensions of monomeric or oligomeric macromolecules by observing their Brownian motion. It requires only a relatively small amount of sample and can be done in various environments. Analyzing the homogeneity of a biomolecular solution at different physiochemical conditions, as concentrations, pH values, and also overtime, before outlining crystallization experiments provides most valuable information to increase the success rate of such experiments to obtain X-ray suitable crystals. Further, light scattering methods can analyze particle size distributions in real time and optionally also in flow mode. The intensity pattern of light scattered by particles in solution is correlated with itself within short time intervals to determine the autocorrelation function, which allows a calculation of the particle diffusion constant, providing the value of the hydrodynamic radii (Rh) via the Stokes-Einstein equation. In this context, DLS is today most widely used for characterizing biomolecular suspensions in different fields of life sciences and biotechnology. In the following, we introduce light scattering techniques and explain the application of light scattering techniques using selected examples.","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image Analysis Methodologies. 图像分析方法。

4区工程技术

Advances in biochemical engineering/biotechnology Pub Date : 2026-03-31 DOI: 10.1007/10_2025_300

Ashwin Kumar Rajagopalan, Daniel Biri

引用次数: 0