Natural Products and Bioprospecting最新文献_第3页

Expanding horizons of iminosugars as broad-spectrum anti-virals: mechanism, efficacy and novel developments 亚氨基糖作为广谱抗病毒药物的前景：机制、功效和新发展。

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-09-26 DOI: 10.1007/s13659-024-00477-5

Qiantong Liu, Yanyun Liu, Tingting Liu, Jinbao Fan, Zanxian Xia, Yingjun Zhou, Xu Deng

{"title":"Expanding horizons of iminosugars as broad-spectrum anti-virals: mechanism, efficacy and novel developments","authors":"Qiantong Liu, Yanyun Liu, Tingting Liu, Jinbao Fan, Zanxian Xia, Yingjun Zhou, Xu Deng","doi":"10.1007/s13659-024-00477-5","DOIUrl":"10.1007/s13659-024-00477-5","url":null,"abstract":"<div><p>Iminosugars, a class of polyhydroxylated cyclic alkaloids with intriguing properties, hold promising therapeutic potentials against a broad spectrum of enveloped viruses, including DENV, HCV, HIV, and influenza viruses. Mechanistically, iminosugars act as the competitive inhibitors of host endoplasmic reticular α-glucosidases I and II to disrupt the proper folding of viral nascent glycoproteins, which thereby exerts antiviral effects. Remarkably, the glycoproteins of many enveloped viruses are significantly more dependent on the calnexin pathway of the protein folding than most host glycoproteins. Therefore, extensive interests and efforts have been devoted to exploit iminosugars as broad-spectrum antiviral agents. This review provides the summary and insights into the recent advancements in the development of novel iminosugars as effective and selective antiviral agents against a variety of enveloped viruses, as well as the understandings of their antiviral mechanisms.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in pharmaceutical cocrystals of theophylline 茶碱药用共晶体的最新进展

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-09-14 DOI: 10.1007/s13659-024-00470-y

Yanxiao Jia, Dezhi Yang, Wenwen Wang, Kun Hu, Min Yan, Li Zhang, Li Gao, Yang Lu

{"title":"Recent advances in pharmaceutical cocrystals of theophylline","authors":"Yanxiao Jia, Dezhi Yang, Wenwen Wang, Kun Hu, Min Yan, Li Zhang, Li Gao, Yang Lu","doi":"10.1007/s13659-024-00470-y","DOIUrl":"10.1007/s13659-024-00470-y","url":null,"abstract":"<div><p>Currently, cocrystallization is a promising strategy for tailoring the physicochemical properties of active pharmaceutical ingredients. Theophylline, an alkaloid and the most primary metabolite of caffeine, is a readily available compound found in tea and coffee. It functions primarily as a bronchodilator and respiratory stimulant, making it a mainstay treatment for lung diseases like asthma. Theophylline’s additional potential benefits, including anti-inflammatory and anticancer properties, and its possible role in neurological disorders, have garnered significant research interest. Cocrystal formation presents a viable approach to improve the physicochemical properties of theophylline and potentially mitigate its toxic effects. This review comprehensively explores several successful studies that utilized cocrystallization to favorably alter the physicochemical properties of theophylline or its CCF. Notably, cocrystals can not only enhance the solubility and bioavailability of theophylline but also exhibit synergistic effects with other APIs. The review further delves into the hydrogen bonding sites within the theophylline structure and the hydrogen bonding networks observed in cocrystal structures.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13659-024-00470-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grewiifopenes A–K, bioactive clerodane diterpenoids from Casearia grewiifolia Vent. Grewiifopenes A-K, bioactive clerodane diterpenoids from Casearia grewiifolia Vent.

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-09-14 DOI: 10.1007/s13659-024-00475-7

Phanruethai Pailee, Paratchata Batsomboon, Wiriya Yaosanit, Theerawat Thananthaisong, Chulabhorn Mahidol, Poonsakdi Ploypradith, Nanthawan Reuk-ngam, Panita Khlaychan, Supanna Techasakul, Somsak Ruchirawat, Vilailak Prachyawarakorn

{"title":"Grewiifopenes A–K, bioactive clerodane diterpenoids from Casearia grewiifolia Vent.","authors":"Phanruethai Pailee, Paratchata Batsomboon, Wiriya Yaosanit, Theerawat Thananthaisong, Chulabhorn Mahidol, Poonsakdi Ploypradith, Nanthawan Reuk-ngam, Panita Khlaychan, Supanna Techasakul, Somsak Ruchirawat, Vilailak Prachyawarakorn","doi":"10.1007/s13659-024-00475-7","DOIUrl":"10.1007/s13659-024-00475-7","url":null,"abstract":"<div><p>Eleven novel clerodane-type diterpenoids, grewiifopenes A–K (<b>1</b>–<b>4</b> and <b>12</b>–<b>18</b>), along with nine known compounds (<b>5</b>–<b>11</b>, <b>19</b>, and <b>20</b>) were purified from the dichloromethane extract of the twigs and stems of <i>Casearia grewiifolia</i> Vent. (Salicaceae). Their spectroscopic data, including the NMR, HRESIMS, and electronic circular dichroism calculations were employed to completely characterize and elucidate the chemical structures and absolute configurations. The clerodane diterpenoids possessing a 6-OH group and no substitution at C-7 exhibited greater cytotoxic activity than others, with their IC<sub>50</sub> values ranging from 0.3 to 2.9 μM. Isocaseamembrin E (<b>7</b>) exhibited antibacterial activity against <i>Staphylococcus aureus</i>, while isocaseamembrin E (<b>7</b>), corymbulosin X (<b>8</b>), caseargrewiin A (<b>9</b>), kurzipene A (<b>10</b>), and balanspene F (<b>11</b>) exhibited antibacterial activity against <i>Bacillus cereus</i>.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13659-024-00475-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata 红豆杉中两种中性多糖的结构特征及对树突状细胞的免疫活性

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-09-14 DOI: 10.1007/s13659-024-00476-6

Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu

{"title":"Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata","authors":"Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu","doi":"10.1007/s13659-024-00476-6","DOIUrl":"10.1007/s13659-024-00476-6","url":null,"abstract":"<div><p><i>Dictyophora rubrovalvata</i> is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from <i>D. rubrovalvata</i>. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 10<sup>3</sup> and 1.25 × 10<sup>4</sup> Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-<i>α</i>-D-Gal<i>p</i>-(1 → 6)-<i>α</i>-D-Gal<i>p</i>-(2,1 → 6)-<i>α</i>-D-Man<i>p</i>-(2,1 → 6)-<i>α</i>-D-Gal<i>p</i>-(1, and branch chains were <i>β</i>-D-Xyl<i>p</i>-(1 → 3)-<i>α</i>-L-Fuc<i>p</i>-(1 → 4)-<i>α</i>-D-Man<i>p</i>-(1 → and <i>α</i>-D-Gal<i>p</i>-(1 → 3)-<i>α</i>-D-Gal<i>p</i>-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13659-024-00476-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro-scale screening of genetically modified Fusarium fujikuroi strain extends the apicidin family 转基因 Fusarium fujikuroi 菌株的微尺度筛选扩展了凋萎素家族。

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-08-23 DOI: 10.1007/s13659-024-00473-9

Alica Fischle, Mika Lutsch, Florian Hübner, Linda Schäker-Hübner, Lina Schürmann, Finn K. Hansen, Svetlana A. Kalinina

{"title":"Micro-scale screening of genetically modified Fusarium fujikuroi strain extends the apicidin family","authors":"Alica Fischle, Mika Lutsch, Florian Hübner, Linda Schäker-Hübner, Lina Schürmann, Finn K. Hansen, Svetlana A. Kalinina","doi":"10.1007/s13659-024-00473-9","DOIUrl":"10.1007/s13659-024-00473-9","url":null,"abstract":"<div><p>Apicidins are a class of naturally occurring cyclic tetrapeptides produced by few strains within the <i>Fusarium</i> genus. These secondary metabolites have gained significant attention due to their antiprotozoal activity through HDAC inhibition, thereby highlighting their potential for the treatment of malaria. Predominantly, apicidins have been isolated from <i>Fusarium semitectum</i>, offering a deep insight into the biosynthetic pathway responsible for their formation. A similar biosynthetic gene cluster has also been identified in the rice pathogenic fungus <i>F. fujikuroi</i>, leading the discovery of three additional apicidins through genetic manipulation. Routine mass spectrometric screening of these compound-producing strains revealed another metabolite structurally related to previously studied apicidins. By optimizing culture conditions and developing an effective isolation method, we obtained a highly pure substance, whose chemical structure was fully elucidated using NMR and HRMS fragmentation. Further studies were conducted to determine cytotoxicity, antimalarial activity, and HDAC inhibitory activity of this new secondary metabolite alongside the previously known apicidins. This work not only expands the apicidin class with a new member but also provides extensive insights and comparative analysis of apicidin-like substances produced by <i>F. fujikuroi</i>.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A 利用表面改性介孔二氧化硅 Santa Barbara Amorphous 15 纯化和固定化β-葡萄糖苷酶，以环保方式制备沙葛苷 A。

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-08-23 DOI: 10.1007/s13659-024-00471-x

Ya-Ya Yang, Shun-Li Jing, Jia-Li Shao, Ji-Xuan Chen, Wei-Feng Zhang, Si-Yuan Wan, Yu-Ping Shen, Huan Yang, Wei Yu

{"title":"Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A","authors":"Ya-Ya Yang, Shun-Li Jing, Jia-Li Shao, Ji-Xuan Chen, Wei-Feng Zhang, Si-Yuan Wan, Yu-Ping Shen, Huan Yang, Wei Yu","doi":"10.1007/s13659-024-00471-x","DOIUrl":"10.1007/s13659-024-00471-x","url":null,"abstract":"<div><p>Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl-<i>γ</i>-aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant <i>β</i>-glucosidase from <i>Terrabacter ginsenosidimutans</i> (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the power of Arctium lappa root: a review of its pharmacological properties and therapeutic applications 利用牛蒡根的力量：药理特性和治疗应用综述。

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-08-20 DOI: 10.1007/s13659-024-00466-8

Mukul Shyam, Evan Prince Sabina

{"title":"Harnessing the power of Arctium lappa root: a review of its pharmacological properties and therapeutic applications","authors":"Mukul Shyam, Evan Prince Sabina","doi":"10.1007/s13659-024-00466-8","DOIUrl":"10.1007/s13659-024-00466-8","url":null,"abstract":"<div><p><i>Arctium lappa,</i> widely recognized as burdock, is a perennial plant that is employed in the realm of traditional Chinese medicine for a wide range of medicinal applications. The herb is rich in bioactive metabolites with therapeutic potential, encompassing polyphenolic antioxidants in its leaves, and flavonoids and fructo-oligosaccharides in its underground parts. Nutraceuticals originating from botanical sources such as <i>Arctium lappa</i> provide supplementary health advantages alongside their nutritional content and have demonstrated effectiveness in the prevention and management of specific ailments. The utilization of <i>Arctium lappa</i> root extract has exhibited encouraging outcomes in addressing hepatotoxicity induced by cadmium, lead, chromium, and acetaminophen, ameliorating liver damage and oxidative stress. Additionally, the root extract displays properties such as antidiabetic, hypolipidemic, aphrodisiac, anti-rheumatic, anti-Alzheimer, and various other pharmacological actions.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11335715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation of selected secondary metabolites by Alternaria species and the pharmaceutical, food and agricultural application of biotransformation products Alternaria 物种对某些次级代谢物的生物转化以及生物转化产物在制药、食品和农业方面的应用。

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-08-19 DOI: 10.1007/s13659-024-00469-5

Babalwa Tembeni, Olusola Emmanuel Idowu, Rachid Benrkia, Salima Boutahiri, Opeyemi Joshua Olatunji

{"title":"Biotransformation of selected secondary metabolites by Alternaria species and the pharmaceutical, food and agricultural application of biotransformation products","authors":"Babalwa Tembeni, Olusola Emmanuel Idowu, Rachid Benrkia, Salima Boutahiri, Opeyemi Joshua Olatunji","doi":"10.1007/s13659-024-00469-5","DOIUrl":"10.1007/s13659-024-00469-5","url":null,"abstract":"<div><p>Biotransformation is a process in which molecules are modified in the presence of a biocatalyst or enzymes, as well as the metabolic alterations that occur in organisms from exposure to the molecules. Microbial biotransformation is an important process in natural product drug discovery as novel compounds are biosynthesised. Additionally, biotransformation products offer compounds with improved efficacy, solubility, reduced cytotoxic and allows for the understanding of structure activity relationships. One of the driving forces for these impeccable findings are associated with the presence of cytochrome P450 monooxygenases that is present in all organisms such as mammals, bacteria, and fungi. Numerous fungal strains have been used and reported for their ability to biotransform different compounds. This review focused on studies using <i>Alternaria</i> species as biocatalysts in the biotransformation of natural product compounds. <i>Alternaria</i> species facilitates reactions that favour stereoselectivity, regioselectivity under mild conditions. Additionally, microbial biotransformation products, their application in food, pharmaceutical and agricultural sector is discussed in this review.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11333692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharide fraction from Triplostegia glandulifera Wall and its renoprotective effect in streptozotocin-induced diabetic mice by attenuating oxidative stress 从三叶一枝花壁中提取的多糖成分及其通过减轻氧化应激对链脲佐菌素诱导的糖尿病小鼠的肾保护作用。

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-08-19 DOI: 10.1007/s13659-024-00467-7

Hai-Hui Guo, Lei Wu, Dan Mi, Xing-Yu Zhang, Fu-Mei He, Ting Lei, Fu-Sheng Wang

{"title":"Polysaccharide fraction from Triplostegia glandulifera Wall and its renoprotective effect in streptozotocin-induced diabetic mice by attenuating oxidative stress","authors":"Hai-Hui Guo, Lei Wu, Dan Mi, Xing-Yu Zhang, Fu-Mei He, Ting Lei, Fu-Sheng Wang","doi":"10.1007/s13659-024-00467-7","DOIUrl":"10.1007/s13659-024-00467-7","url":null,"abstract":"<div><p><i>Triplostegia glandulifera</i> Wall (<i>T. glandulifera</i>) is an ethnomedicine commonly used by ethnic minorities in Yunnan, China, to treat kidney disease. However, there are few reports on the renoprotective effects of this substance, and the active ingredients remain unclear. In this study, we extracted the polysaccharide fractions TGB and TGC using the water extraction-alcohol precipitation method and determined their molecular weight (Mw) and monosaccharide composition. The study investigated the protective effects of TGB and TGC fractions against diabetic nephropathy (DN) using an in vitro high glucose-induced HRMCs model and an in vivo STZ-induced diabetic mouse model. HPLC analysis revealed that TGB contained D-galacturonic acid, D-glucose, D-galactose, and D-arabinose, and had a lower Mw than TGC. In vitro, TGB showed concentration-dependent antioxidant activity and effectively reduced abnormal proliferation and while attenuating oxidative stress in HRMCs. In mice with diabetes, TGB corrected the dysregulation of glucose-lipid metabolism and alleviated oxidative stress in the kidneys. Additionally, it improved renal function and reduced renal tissue damage. The study suggests that the low Mw polysaccharides (TGB) have better activity against DN through the antioxidative stress mechanism.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11333391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural and revolutionary tumor-specific T-cell therapy 天然、革命性的肿瘤特异性 T 细胞疗法。

IF 4.8 3区化学

Natural Products and Bioprospecting Pub Date : 2024-08-19 DOI: 10.1007/s13659-024-00472-w

Zhi Dai, Xue-Meng Liu, Yun-li Zhao, Li-Xing Zhao, Xiao-Dong Luo

{"title":"Natural and revolutionary tumor-specific T-cell therapy","authors":"Zhi Dai, Xue-Meng Liu, Yun-li Zhao, Li-Xing Zhao, Xiao-Dong Luo","doi":"10.1007/s13659-024-00472-w","DOIUrl":"10.1007/s13659-024-00472-w","url":null,"abstract":"<div><p>Recently the FDA conducted a risk investigation and labeled the <b>Boxed Warning</b> for all BCMA- and CD19-directed CAR-T cell therapy, so does it mean that the public must take risk of secondary cancer to receive cell therapy? Here, without lentivirus and professional antigen presenting cell application, a novel tumor-specific T-cell therapy was successfully developed only by co-culturing MHC<sup>+</sup> cancer cells and Naïve-T cells under the CD28 co-stimulatory signals. These tumor-specific T-cells could be separated through cell size and abundantly produced from peripheral blood, and would spontaneously attack target cells that carrying the same tumor antigen while avoiding others in vitro test. Moreover, it markedly decreased 90% tumor nodules companying with greatly improving overall survival (76 days <i>vs</i> 30 days) after twice infusion back to mice. This work maximally avoided the risks of secondary cancer and non-specific killing, and might open a revolutionary beginning of natural tumor-specific T-cell therapy.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"14 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11333775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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