Natural Products and Bioprospecting最新文献

{"title":"Combined treatment with naringin and osthole ameliorates colitis through microbiota–amino acid metabolism and the JNK pathway","authors":"Mengqin Chen,&nbsp;Zihao Lu,&nbsp;Tong Zhang,&nbsp;Guoping Li,&nbsp;Qingyu Zheng,&nbsp;Tao Zhang","doi":"10.1007/s13659-025-00582-z","DOIUrl":"10.1007/s13659-025-00582-z","url":null,"abstract":"<div><p>Inflammatory bowel disease (IBD), particularly ulcerative colitis, involves disruption of the intestinal mucosal barrier due to ecological and metabolic imbalances in the gut as its underlying pathology. Current therapies for Ulcerative colitis (UC) exhibit limited efficacy and adverse effects, necessitating the development of novel treatment strategies. Naringin and osthole are natural herbal compounds that show therapeutic potential in various inflammatory models due to their excellent anti-inflammatory activity. However, their combined therapeutic effects and precise mechanisms in UC remain unreported. This study aimed to explore the therapeutic effectiveness and mechanism of naringin combined with osthole in addressing dextran sodium sulfate (DSS)-induced colitis. The investigation centered on their impact on the disruption of the intestinal epithelial cell barrier, modulation of intestinal flora composition, alteration of metabolites, and inflammation model in vitro. Modal assessment encompassed body weight, disease activity index (DAI) score, colon length, and histopathological examination. Intestinal barrier integrity was evaluated through Quantitative Real-Time PCR, western blotting, and immunofluorescence staining. Microbiota abundance and metabolic levels were assessed using 16S ribosomal RNA gene sequencing and metabolomics analysis. Protein expression levels of pertinent pathways and associated receptors were tested through network pharmacology prediction and western blot analysis. Naringin and osthole synergistically relieved colitis symptoms in mice compared with either drug alone or 5-aminosalicylic acid, as evidenced by weight loss recovery, DAI scores, and colon length preservation. Mechanistically, naringin combined with osthole down-regulated the expression level of JNK/NF-κB signaling pathway related proteins and repaired intestinal barrier. Furthermore, the combination regulates the composition of the microflora and promotes the restoration of a steady state of the microflora. Metabolomic revealed amino acid-tryptophan metabolism as a key metabolic pathway. It also reveals the microbiota-tryptophan pathway as a potential therapeutic strategy. Naringin combined with osthole can alleviate DSS-induced colitis more effectively by JNK/NF-κB signaling pathway, repairing barrier function and regulating intestinal microbiota and metabolites. These findings provide a theoretical basis for the combination therapy strategy to enhance the efficacy of potential functional food in treating ulcerative colitis.</p></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12868363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrimidine-containing natural products: occurrences and biological activities","authors":"Jian-Neng Yao,&nbsp;Yongjie Zhu,&nbsp;He-Ping Chen,&nbsp;Yihua Chen","doi":"10.1007/s13659-025-00583-y","DOIUrl":"10.1007/s13659-025-00583-y","url":null,"abstract":"<div><p>Nitrogen-containing core structure pyrimidine has long been regarded as one of the privileged scaffolds for novel drug development. Natural products embedded with pyrimidine motifs are distinguished by their exceptional scaffold diversity and vast structural complexity, which endow them with versatile biological activities, including anticancer, antiviral, antifungal and anti-inflammatory activities. This review is dedicated to surveying a series of structurally distinctive naturally occurring compounds characterized by the presence of an aromatic heterocyclic pyrimidine moiety covering from 2004 to early 2025. Multiple key aspects of these 156 pyrimidine-containing compounds, including natural sources, features of chemical structure, biological activities, as well as biosynthetic studies are summarized. The review emphasizes the enduring potential of natural products, highlighting their inherent capacity for medication and optimization to fuel the pipeline of lead compound targeting major disease and providing new window for overview of these pyrimidine-containing natural products. Overall, this review aims to unlocking the potential of these isolates. It thus offers a fresh reference for the exploitation of pyrimidine-containing natural products, with the ultimate goal of realizing their therapeutic potential.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12868415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new 14-noreudesmane-type sesquiterpenoids from the roots of Hippophae rhamnoides","authors":"Fatima Abdurrahman Galadanchi,&nbsp;Polina Lopukhina,&nbsp;Sisi Bai,&nbsp;Guohao Dong,&nbsp;Zhongyu Zhou,&nbsp;Haihui Xie,&nbsp;Xiaoyi Wei","doi":"10.1007/s13659-025-00581-0","DOIUrl":"10.1007/s13659-025-00581-0","url":null,"abstract":"<div><p><i>Hippophae rhamnoides</i> L. (Elaeagnaceae), commonly known as sea buckthorn, is a medicinal plant valued for its diverse bioactive constituents and broad therapeutic potential. Phytochemical investigation of its roots led to the isolation of three new 14-noreudesmane-type sesquiterpenoids (<b>1</b>–<b>3</b>), together with sixteen known compounds (<b>4</b>–<b>19</b>). Their structures and absolute configurations were determined using comprehensive spectroscopic analyses and quantum chemical computations of electronic circular dichroism (ECD) spectra and ¹³C NMR shifts. Three new sesquiterpenoids (<b>1</b>–<b>3</b>) were tested for their antioxidant, anti-inflammatory, antibacterial, and <i>α</i>-glucosidase inhibitory activities. Unfortunately, none exhibited significant activity under the tested conditions. Among the isolated known compounds, all those displaying <i>α</i>-glucosidase inhibitory and antibacterial activities are pentacyclic triterpenoids. Hippophamide (<b>17</b>) showed significant DPPH and ABTS radical-scavenging activity. These results enriched the chemical profile of <i>H. rhamnoides</i> roots.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12868457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4","authors":"Jie-Chun Zeng,&nbsp;Xu-Ping Zhang,&nbsp;Lu Gao,&nbsp;Qian-Qian Yin,&nbsp;Wei-Guang Wang","doi":"10.1007/s13659-025-00580-1","DOIUrl":"10.1007/s13659-025-00580-1","url":null,"abstract":"<div><p>Five new <i>α</i>-pyrones, diaporpyrones G-K (<b>1</b>–<b>5</b>) and three new <i>γ</i>-butenolide derivatives, porbutenolides A-C (<b>6</b>–<b>8</b>), along with seven known compounds (<b>9</b>–<b>15</b>), were isolated from the culture extract of the endophytic fungus <i>Diaporthe</i> sp. CCY4. Their structures were elucidated by comprehensive spectroscopic analysis, including 1D/2D NMR and HRESIMS data. The absolute configurations of <b>7</b> and <b>8</b> were assigned using electronic circular dichroism (ECD) calculations. All compounds were evaluated for inhibitory activity against ubiquitin-specific peptidase 4 (USP4). Compounds <b>2</b>,<b> 5</b>,<b> 9</b>, and <b>13</b> exhibited significant anti-ubiquitination effects at 40 μM, with <b>13</b> showing the most potent inhibition (IC₅₀ = 20.85 μM).</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12864556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome mining and functional characterization of four type I sesquiterpene synthases from the Tiger Milk Mushroom Lignosus rhinocerus","authors":"Ming-Xuan Gao,&nbsp;Li-Li Guo,&nbsp;Meng-Ting Wang,&nbsp;Xin-Yi Zhang,&nbsp;Xinyang Li,&nbsp;Shin-Yee Fung,&nbsp;He-Ping Chen,&nbsp;Ji-Kai Liu","doi":"10.1007/s13659-025-00578-9","DOIUrl":"10.1007/s13659-025-00578-9","url":null,"abstract":"<div><p>The Tiger Milk mushrooms (<i>Lignosus</i> spp.) have long been used as traditional folk medicines throughout Southeast Asia. However, the chemical constituents of these species remain largely unexplored. In this study, four type I sesquiterpene synthases (LrhTS1−LrhTS4) from <i>Lignosus rhinocerus</i> TM02^® were functionally characterized through genome mining, in vitro enzymatic assays, and heterologous expression in an <i>Escherichia coli</i> host engineered to overexpress an exogenous mevalonate pathway. The enzyme products were analyzed by GC−MS and further purified and structurally elucidated by NMR spectroscopy. A total of 11 sesquiterpenes (<b>1</b>−<b>11</b>) were identified, among which two were unstable and converted to stable derivatives (<b>2</b> to <b>2a</b> and <b>7</b> to <b>7a</b>). Notably, compounds <b>1</b>−<b>10</b> are reported from the genus <i>Lignosus</i> for the first time. LrhTS1 and LrhTS3 exhibited high catalytic specificity, whereas LrhTS2 and LrhTS4 displayed product promiscuity. This work expands the knowledge of terpene synthase from mushroom, and advances the understanding of the chemical basis underlying the bioactivity of the <i>Lignosus rhinocerus</i> TM02®.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12864622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydosetins & pullularins—bioactive tetramic acids & cyclodepsipeptides from the endophytic and nematophagous fungus Polydomus karssenii","authors":"Natalia A. Llanos-López,&nbsp;Jan-Peer Wennrich,&nbsp;Janette Miled,&nbsp;Samad Ashrafi,&nbsp;Wolfgang Maier,&nbsp;Frank Surup,&nbsp;Marc Stadler","doi":"10.1007/s13659-025-00579-8","DOIUrl":"10.1007/s13659-025-00579-8","url":null,"abstract":"<div><p>In course of investigating the endophytic and nematode-associated fungus <i>Polydomus karssenii</i> for the production of secondary metabolites, seven previously undescribed natural products were isolated from liquid and solid-state fermentations. 1D and 2D NMR spectroscopy, together with HR-ESI–MS data, enabled the elucidation of the planar structures of 3-decalinoyltetramic acids polydosetins A–E (<b>1</b>–<b>5</b>) and cyclodepsipeptides pullularins G and H (<b>6</b> and <b>7</b>). The relative configurations of the decalin moiety of <b>1</b>–<b>5</b> were determined based on ROESY correlations and ¹H–¹H coupling constants. The configuration of the side chains was established through a detailed <i>J</i>-resolved analysis (Murata’s method) in combination with chemical shift comparison to model compounds. Absolute stereochemistry of <b>1</b>–<b>5</b> was assigned based on ECD data, and confirmed by Mosher’s method utilizing <b>3</b>. Finally, the absolute configuration of amino acid residues in <b>6</b> and <b>7</b> was determined through advanced Marfey’s method. Bioassays revealed that compounds <b>1</b>, <b>3</b>, <b>5</b>, and <b>7</b> were active against Gram-positive bacteria, <b>3</b> and <b>5</b> exhibited antifungal activity, and <b>1</b> and <b>2</b> showed nematicidal effects. These results underscore the untapped chemical potential of <i>P. karssenii</i> and highlight the importance of exploring nematode-associated fungi as sources of new natural products with potential antimicrobial and nematicidal properties.</p></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12864567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Integrin β3: novel antiplatelet lignans 6’-Hydroxyjusticidin B and Neojusticin A from Justicia procumbens unveiled via multi-omics and biophysical validation","authors":"Meixian Xiang,&nbsp;Songtao Wu,&nbsp;Hanxiang Mei,&nbsp;Xiang Zheng,&nbsp;Cong Wang","doi":"10.1007/s13659-025-00577-w","DOIUrl":"10.1007/s13659-025-00577-w","url":null,"abstract":"<div><p>Thrombotic disorders remain a global health burden, necessitating novel antiplatelet agents with improved safety and efficacy. This study investigates the molecular mechanisms of two lignans, 6'-Hydroxyjusticidin B (6'-HJB) and Neojusticin A (Neo-A), isolated from <i>Justicia procumbens</i> L., through an innovative target-driven strategy integrating LC/MS, proteomics, network pharmacology, and biophysical validation. For the first time, integrin β₃ (ITGB3) was identified as their direct molecular target, with microscale thermophoresis (MST) confirming high-affinity binding, the dissociation constant (Kd) = 0.0642 ± 0.005 μM for 6'-HJB; 0.0097 ± 0.001 μM for Neo-A. This study not only elucidates the structural basis of their activity-C-6 hydroxylation in 6'-HJB enhances ITGB3 specificity, whereas Neo-A’s fused furan ring optimizes COX-1 interaction, but also establishes a paradigm shift from phenotypic screening to target-validated natural product research. The findings position 6'-HJB and Neo-A as promising candidates for the development of safer, ITGB3-mediated antithrombotic therapies, with future efforts directed toward structural optimization and preclinical validation.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12864604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mining anticoagulant peptides from Poecilobdella manillensis by peptidomics analysis","authors":"Han-xue Zheng,&nbsp;Xiao-li Deng,&nbsp;Teng-teng Li,&nbsp;Guo-hua Xia,&nbsp;Huan Yang,&nbsp;Jiang-song Peng,&nbsp;Yu-ping Shen","doi":"10.1007/s13659-025-00573-0","DOIUrl":"10.1007/s13659-025-00573-0","url":null,"abstract":"<div><p>Thrombosis triggers various severe diseases, while antithrombotic drugs carry bleeding risks, making the development of novel natural anticoagulants a subject of widespread attention. <i>Poecilobdella manillensis</i>, a prevalent medicinal leech, exhibits remarkable anticoagulant and antithrombotic activities. However, the material basis underlying its anticoagulant effects remains insufficiently investigated. This study aims to mine anticoagulant peptides from <i>P. manillensis</i> by peptidomics analysis, elucidate the material basis of its anticoagulant activity, and provide candidate molecules for developing novel natural anticoagulant drugs. Proteins extracted from <i>P. manillensis</i> were enzymatically digested and fractionated using DEAE-52 and CN columns. The resulting peptide components were analyzed by UPLC-Q-Orbitrap HRMS, and peptide sequences were matched against proteomic databases using Proteome Discoverer. Anticoagulant peptides were predicted using the BIOPEP-UWM database and PeptideRanker server, followed by in vitro and in vivo activity validation. Results showed that the hydrolysate consisted predominantly of low-molecular-weight peptides. 1533 peptides with Mw &lt; 3000 Da (length &lt; 20 amino acids) were identified from the PM-A2 and PM-A3 fractions, accounting for 40.76% of the total. Four peptides selected through predictive screening demonstrated anticoagulant and antithrombotic activities in vitro. Among them, LE-11 significantly prolonged both APTT and TT (<i>P</i> &lt; 0.0001). Furthermore, LE-11 effectively alleviated carrageenan-induced thrombosis in mice, outperforming the heparin control at the mid-concentration (20 mg/kg). In this study, the highly active anticoagulant peptide LE-11 was identified from <i>P. manillensis</i> through peptidomic analysis. These findings establish a solid foundation for developing anticoagulant drugs from this source and provide critical scientific support for its clinical application in treating thrombotic diseases.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12862037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clerodane diterpenoid glycosides from the tuberous roots of Paratinospora sagittata: targeted isolation, structure characterization and immunomodulatory properties","authors":"Jun-Sheng Zhang,&nbsp;Rui Ao,&nbsp;Yin-Bo Pan,&nbsp;Xin-Cheng Zhuang,&nbsp;Yi-Ke Yin,&nbsp;Jie Bao,&nbsp;Hua Zhang","doi":"10.1007/s13659-025-00555-2","DOIUrl":"10.1007/s13659-025-00555-2","url":null,"abstract":"<div><p>Guided by the MS/MS-based molecular networking, eight previously undescribed clerodane diterpenoid glycosides, designated tinospinosides F–M (<b>1</b>−<b>8</b>), along with 12 known analogues (<b>9</b>−<b>20</b>), were isolated from the tuberous roots of <i>Paratinospora sagittata</i>. Structural elucidation of the undescribed compounds was achieved through comprehensive spectroscopic analyses (NMR, HRESIMS), with their absolute configurations confirmed via single-crystal X-ray diffraction, TD-DFT/ECD computational analyses, and chemical degradation. Immunomodulation evaluation on all the isolates revealed that compounds <b>6</b> and <b>7</b> exerted significant promoting effect toward NO production in RAW264.7 macrophages. Further study demonstrated that <b>6</b> could enhance the release of immune cytokines (e.g., TNF-<i>α</i>) and upregulate the protein expression of iNOS and COX-2, which was potentially mediated through the activation of NF-κB signaling pathway.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12862036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cortinarius mapuveronicae from South America, a chemical and morphological link between European and Australian dermocyboid Cortinarii","authors":"Josefine Lange,&nbsp;Lesley Huymann,&nbsp;Sophie Schwarzkopf,&nbsp;Dilara Balci,&nbsp;Mehdi D. Davari,&nbsp;Arijana Turanovic,&nbsp;Clemens Gotsis,&nbsp;Götz Palfner,&nbsp;Bianka Siewert,&nbsp;Ursula Peintner,&nbsp;Norbert Arnold","doi":"10.1007/s13659-025-00552-5","DOIUrl":"10.1007/s13659-025-00552-5","url":null,"abstract":"<div><p>The new species <i>Cortinarius mapuveronicae</i> from Andean-Patagonian <i>Nothofagus</i>-forests is described in a polythetic approach combining chemical analysis of the anthraquinonoid secondary metabolites, microscopical and morphological characteristics, as well as molecular phylogeny. <i>C. mapuveronicae</i> exhibits an intense red color reaction of the basidiomata by treatment with KOH, whereas the basidiospores are turning purplish brown. As responsible compound, the new anthraquinonoid pigment clavorubin-8-<i>O</i>-methylether (<b>1</b>), together with the known monomeric and dimeric anthraquinones (+)-7,7-emodinphyscion (<b>2</b>), emodin (<b>3</b>), emodin-6,8-di-<i>O</i>-methylether (<b>4</b>), questin (<b>5</b>), (+)-(<i>S</i>)-skyrin (<b>6</b>), (+)-(<i>S</i>)-aurantioskyrin (<b>7</b>), hypericin (<b>8</b>), dermolutein (<b>9</b>), and endocrocin (<b>10</b>) could be identified, showing also remarkable activity in a (photo)antimicrobial and (photo)cytotoxic assay. Phylogenetic analysis (ITS, LSU, rpb1) demonstrates a sister group relationship with the holotype of <i>C. rubrobasalis</i>.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12862044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}