红豆杉中两种中性多糖的结构特征及对树突状细胞的免疫活性

IF 4.8 3区 化学 Q1 CHEMISTRY, MEDICINAL
Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu
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引用次数: 0

摘要

红豆杉(Dictyophora rubrovalvata)是一种药食同源的珍贵真菌,其多糖因具有丰富的活性而日益受到人们的关注。然而,人们对其均质多糖的结构和活性研究还不够。本研究从 D. rubrovalvata 中纯化了两种多糖 DRP-I 和 DRP-II。通过化学成分、单糖成分分析、甲基化分析和核磁共振波谱对它们的结构进行了表征。结果表明,DRP-I 和 DRP-II 为中性杂多糖,分子量分别为 5.79 × 103 和 1.25 × 104 Da,由甘露糖、半乳糖、葡萄糖、木糖和岩藻糖组成。主链为 → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1),支链为β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → 1)和α-D-Galp-(1 → 3)-α-D-Galp-(1 → 1)。对树突状细胞进行的体外免疫活性试验表明,DRP-I 和 DRP-II 能上调 IL-10 和 IL-6 的表达,并能抑制 TNF-α 的表达,其表达呈浓度依赖性。这项研究表明,DRP-I和DRP-II具有平衡过度炎症的免疫活性,而分子量是影响免疫活性的重要因素。 图文摘要
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 103 and 1.25 × 104 Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1, and branch chains were β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → and α-D-Galp-(1 → 3)-α-D-Galp-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.

Graphical Abstract

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来源期刊
Natural Products and Bioprospecting
Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-
CiteScore
8.30
自引率
2.10%
发文量
39
审稿时长
13 weeks
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