Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu
{"title":"红豆杉中两种中性多糖的结构特征及对树突状细胞的免疫活性","authors":"Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu","doi":"10.1007/s13659-024-00476-6","DOIUrl":null,"url":null,"abstract":"<div><p><i>Dictyophora rubrovalvata</i> is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from <i>D. rubrovalvata</i>. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 10<sup>3</sup> and 1.25 × 10<sup>4</sup> Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-<i>α</i>-D-Gal<i>p</i>-(1 → 6)-<i>α</i>-D-Gal<i>p</i>-(2,1 → 6)-<i>α</i>-D-Man<i>p</i>-(2,1 → 6)-<i>α</i>-D-Gal<i>p</i>-(1, and branch chains were <i>β</i>-D-Xyl<i>p</i>-(1 → 3)-<i>α</i>-L-Fuc<i>p</i>-(1 → 4)-<i>α</i>-D-Man<i>p</i>-(1 → and <i>α</i>-D-Gal<i>p</i>-(1 → 3)-<i>α</i>-D-Gal<i>p</i>-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13659-024-00476-6.pdf","citationCount":"0","resultStr":"{\"title\":\"Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata\",\"authors\":\"Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu\",\"doi\":\"10.1007/s13659-024-00476-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Dictyophora rubrovalvata</i> is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from <i>D. rubrovalvata</i>. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 10<sup>3</sup> and 1.25 × 10<sup>4</sup> Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-<i>α</i>-D-Gal<i>p</i>-(1 → 6)-<i>α</i>-D-Gal<i>p</i>-(2,1 → 6)-<i>α</i>-D-Man<i>p</i>-(2,1 → 6)-<i>α</i>-D-Gal<i>p</i>-(1, and branch chains were <i>β</i>-D-Xyl<i>p</i>-(1 → 3)-<i>α</i>-L-Fuc<i>p</i>-(1 → 4)-<i>α</i>-D-Man<i>p</i>-(1 → and <i>α</i>-D-Gal<i>p</i>-(1 → 3)-<i>α</i>-D-Gal<i>p</i>-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":718,\"journal\":{\"name\":\"Natural Products and Bioprospecting\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-09-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s13659-024-00476-6.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Natural Products and Bioprospecting\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13659-024-00476-6\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Products and Bioprospecting","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s13659-024-00476-6","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata
Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 103 and 1.25 × 104 Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1, and branch chains were β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → and α-D-Galp-(1 → 3)-α-D-Galp-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.