{"title":"Nanozymes in the Bioeconomy: From the Concept of Use to Practical Application","authors":"A. L. Popov, N. R. Popova","doi":"10.1134/S263516762560227X","DOIUrl":"10.1134/S263516762560227X","url":null,"abstract":"<p>Nanozymes are a class of artificial nanostructures exhibiting enzyme-like activity that are capable of mimicking the functions of natural enzymes while maintaining high stability and functionality across a wide range of operating conditions. Their feasibility for industrial-scale synthesis and unique catalytic properties open up broad prospects for applications in various sectors of the bioeconomy. This review summarizes recent advances and key application areas of nanozymes, ranging from enhancing the bioavailability of feed additives to their use in veterinary practice and human medicine, including antiviral therapy, wound healing, regenerative technologies, cryopreservation, and antitumor treatment. The advantages of nanozymes over protein enzymes are discussed, and current challenges related to the standardization of evaluation methods and the assessment of long-term biological effects are highlighted. Overall, the analysis demonstrates that nanozymes have the potential to become a pivotal tool in the development of sustainable agriculture, environmental protection, and biomedical technologies, as well as in ensuring food and technological security. This review aims to stimulate further research in nanotechnology and biomaterials science and to promote the translation and practical implementation of nanozymes in advanced technologies and applications in Russia.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S62 - S71"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. A. Novikova, E. Yu. Podlasova, N. N. Glushchenko
{"title":"Agrophysiological Response of Spring Barley Plants to Water Deficit under Seed Treatment with Metal Nanoparticles","authors":"A. A. Novikova, E. Yu. Podlasova, N. N. Glushchenko","doi":"10.1134/S2635167625602402","DOIUrl":"10.1134/S2635167625602402","url":null,"abstract":"<p>The effect of the presowing treatment of spring barley seeds with Mn, Zn, and Cu nanoparticles (NPs) on the morphophysiological parameters of plants under osmotic stress is studied. The experiment is conducted under controlled conditions. The effect of NPs on the growth, development, yield elements, and biochemical parameters of barley plants under various irrigation regimes in the tillering and earing phases is evaluated. The results show that the treatment of seeds with NPs has a complex effect on plants, depending on water availability and the phase of development. Under conditions of sufficient moisture, the NPs contribute to an increase in the number of stems and grains per ear by 20 and 16%, respectively, as well as development of the root system. However, when there is a shortage of moisture, the effect of NPs is more complex, manifesting itself in changes in plant height, the number of grains, and the grain weight per ear. Biochemical analysis reveals that under conditions of limited irrigation, the level of lipid peroxidation (LPO) increases relative to the control by 20 and 16%. The accumulation of polyphenols and flavonoids increases similarly (by 50.7 and 52.3%, respectively). The presowing treatment of seeds with NPs contributes to a change in these indicators, which affects the antioxidant protection of plants under stress. Thus, the presowing treatment of spring barley seeds with Mn, Zn, and Cu NPs can have a positive effect on plant growth and productivity under conditions of both sufficient and limited moisture. However, the effect of NPs depends on the phase of plant development and the degree of water deficiency, which underscores the need for further research to optimize the use of nanopreparations in agriculture. The observed effect shows that this method is promising for increasing the drought resistance and yield of crops. Further research is needed to develop optimal application schemes based on agroclimatic conditions.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S211 - S218"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Yu. Stanco, A. V. Kurovsky, A. S. Koshtunkova, B. Saratchandra
{"title":"Germination of Wheat Seedlings Exposed to Nickel Nanoparticles with Sizes of 6, 58, and 171 nm","authors":"A. Yu. Stanco, A. V. Kurovsky, A. S. Koshtunkova, B. Saratchandra","doi":"10.1134/S2635167625602062","DOIUrl":"10.1134/S2635167625602062","url":null,"abstract":"<p>The work shows how the concentration of Ni nanoparticles with mean sizes of 6 ± 2.3, 58 ± 24, and 171 ± 65 nm influences the germination of wheat seedlings (<i>Triticum aestivum</i> L.). At a concentration of 3–30 mg/L, seedling root development weakly depends on the presence of particles in the germination medium, but the growth of shoots is stimulated (shoot length increases by 18–31%). With an increase in the concentration from 30 to 300 mg/L, the addition of larger particles does not inhibit seedling germination, while in the presence of 6-nm nanoparticles the length of roots and shoots decreases by 23 and 61%, respectively, compared to the control. It is also shown that the biological properties in the germination medium are influenced not by aggregation, but by the initial particle size.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S204 - S210"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu. M. Shlyapnikov, E. A. Malakhova, E. A. Shlyapnikova
{"title":"Ultrasensitive Biosensor Systems Based on Magnetic Nanomaterials for Monitoring Pathogens in the Environment","authors":"Yu. M. Shlyapnikov, E. A. Malakhova, E. A. Shlyapnikova","doi":"10.1134/S2635167625601330","DOIUrl":"10.1134/S2635167625601330","url":null,"abstract":"<p>Iron oxide-based magnetic nanoparticles have found widespread application in bioanalytical methods. This review focuses on microarray-based analytical systems that employ micron-sized magnetic particles for signal detection. Their operation is determined by specific intermolecular recognition processes occurring in the surface layer on a scale of several nanometers, which allow these methods to be considered nanotechnological. Bioanalytical tests based on magnetic labels and microarrays are characterized by rapidity (analysis time up to 10 min), ultra-high sensitivity, and multiplexing capability. They are highly cost-effective and easy to implement, and thus hold great promise for solving problems of environmental pathogen monitoring that require a combination of high speed and sensitivity with the ability to automate analysis.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S72 - S82"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"On the Use of a Microbial Fuel Cell for the Post-treatment of Solid Plant Waste Products","authors":"V. K. Ilyin, D. V. Korshunov, A. S. Yanenko","doi":"10.1134/S2635167626600070","DOIUrl":"10.1134/S2635167626600070","url":null,"abstract":"<p>For long-duration interplanetary missions and the establishment of habitable bases on the lunar surface, the presence of regenerative biological life-support systems is a prerequisite for their operation. Microbial fuel cells can be used to remove up to 30% of organic compounds from liquid products of substrate biodegradation. Microbial fuel cells can be used to increase the rate of ammonification in the biodegradation products of potato waste and increase the nitrogen yield in the form of ammonium by 7 times.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S83 - S86"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. A. Samovarova, G. V. Lyamina, A. E. Ilela, A. M. Evgrafov
{"title":"On the Green Synthesis of Zinc Oxide Nanoparticles with Siberian Hogweed (Heracleum sibiricum) Extract","authors":"N. A. Samovarova, G. V. Lyamina, A. E. Ilela, A. M. Evgrafov","doi":"10.1134/S2635167625602104","DOIUrl":"10.1134/S2635167625602104","url":null,"abstract":"<p>Zinc oxide nanoparticles are synthesized from solutions of hydroxide suspensions with the addition of Siberian hogweed (<i>Heraculum sibiricum</i>) extracts in ethanol and dilute hydrochloric acid solutions. The separation of the powders from the suspensions is carried out using two methods: filtration and nanospray drying (Nano-Spray Dryer B-90). The results of X-ray phase analysis show that, after heat treatment at 350°C, the particle composition is mainly represented by the ZnO phase. Based on data from electron microscopy and laser diffraction, we find that the extracts have a significant effect on the morphology of the powder particles obtained through filtration, compared to those obtained through nanospray drying. The synthesis process produces easily dispersible aggregates composed of plate-shaped particles with an average size ranging from 200 to 270 nm and a thickness of less than 5 nm.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S107 - S113"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. N. Glushchenko, O. A. Bogoslovskaya, T. A. Yurina, D. A. Petukhov
{"title":"Nanopriming of Winter Wheat Seeds to Improve Grain Quality","authors":"N. N. Glushchenko, O. A. Bogoslovskaya, T. A. Yurina, D. A. Petukhov","doi":"10.1134/S2635167625602414","DOIUrl":"10.1134/S2635167625602414","url":null,"abstract":"<p>The paper presents the results of a study on the pre-sowing treatment of wheat seeds with metal nanoparticles (NPs) and their compositions in nanopreparations. Laboratory and field trials demonstrated a significant improvement in seed germination, seedling growth, and grain quality following the nanopriming of winter wheat seeds. Nanoparticles of zinc (Zn 1 ppm), copper (Cu 0.00001 ppm), and a composite formulation (Fe 1 ppm + Zn 1 ppm + Cu 0.0001 ppm + Mn 0.01 ppm) enhanced seed germination by 2, 4, and 4%, respectively. Treatment with nanopreparations containing individual NPs (Fe, Zn, Cu) increased seedling dry biomass by 38–49%. Manganese nanoparticles (0.1 ppm) produced the maximum gain (+64%). Root dry mass increased by 144% (Cu NPs 0.001 ppm), shoot height by 20% (Fe NPs 1 ppm, Cu NPs 0.001 ppm), and the length of adventitious roots by 24–60%, depending on the NP type. The quality of wheat grain from seeds treated with nanopreparationswas also improved: crude gluten content increased by 5–6% (Fe NPs 1 ppm, Cu NPs 0.001 ppm), protein content by 2–5%, and vitreosity by 1–2%. Grain moisture decreased from 12.1% (control) to a range of 9.8% (Zn NPs 1 ppm) to 10.7% (Composite NPs). Seed treatment with Zn NPs (1 ppm) reduced <i>Fusarium infestation</i> of grain by 2.25 times, while the composite NPs reduced it by 33%. The obtained results demonstrate the high potential of pre-sowing nanopriming for improving the yield and quality of winter wheat.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S219 - S226"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. D. Polishchuk, G. I. Churilov, V. V. Churilova, D. G. Churilov, Yu. N. Ivanycheva
{"title":"Biopolymer Composites and Nanoscale Systems in Crop Production","authors":"S. D. Polishchuk, G. I. Churilov, V. V. Churilova, D. G. Churilov, Yu. N. Ivanycheva","doi":"10.1134/S263516762560213X","DOIUrl":"10.1134/S263516762560213X","url":null,"abstract":"<p>Highly dispersed nanostructures composed of metal nanoparticles and natural polymers, particularly humic acids, have been shown to enhance crop yields. Nanostructures with high surface energy and their composites smaller than 100 nm can eliminate microbial contaminants and positively influence genetic transformation, plant biochemical activity, and crop quality. The comparative effectiveness of metal nanoparticles and their composites, both as microfertilizers and as stimulants of plant growth and development, has been evaluated. The effect of optimal doses of the nanomaterials has been studied and biochemical investigations have been performed. The developed recommendations for the use of copper nanoparticles, humic acids, and their composites can be implemented in crop production and research settings.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S184 - S192"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. I. Tyurin, V. V. Korenkov, D. Yu. Golovin, V. M. Vasyukov, N. A. Sazhnev, N. R. Kildeeva, E. A. Zaitseva, N. L. Klyachko
{"title":"Investigation of Physical and Mechanical Properties of Chitosan–Fibroin Films by Continuous Nanoindentation Methods","authors":"A. I. Tyurin, V. V. Korenkov, D. Yu. Golovin, V. M. Vasyukov, N. A. Sazhnev, N. R. Kildeeva, E. A. Zaitseva, N. L. Klyachko","doi":"10.1134/S2635167625602323","DOIUrl":"10.1134/S2635167625602323","url":null,"abstract":"<p>The paper presents the results of studying frequency and deformation rate dependencies of mechanical properties of chitosan–fibroin polymer films designed for medical purpose as a substrate for cell proliferation. Nanoindentation in continuous stiffness measurement (CSM) mode employing harmonic addition to applied load and quasi-steady loading in creeping mode were used to study film mechanical properties at different scale levels and deformation rates. Based upon experimental data processing, adequate analytical model of viscoelastic properties of the film was developed.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S176 - S183"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. P. Chebotaryova, O. V. Zakharova, P. A. Baranchikov, G. V. Grigoriev, S. M. Tatarko, A. A. Gusev, T. V. Semenyuk, E. Yu. Koiava
{"title":"Highly Dispersed Blast-Furnace Sludge As a Promising Source of Trace Elements in the Cultivation of Microalgae","authors":"S. P. Chebotaryova, O. V. Zakharova, P. A. Baranchikov, G. V. Grigoriev, S. M. Tatarko, A. A. Gusev, T. V. Semenyuk, E. Yu. Koiava","doi":"10.1134/S2635167625602141","DOIUrl":"10.1134/S2635167625602141","url":null,"abstract":"<p>The effect of blast-furnace sludge containing trace elements (iron, zinc, silicon) on the microalgae <i>Chlorella fusca</i>, <i>Chlorella vulgaris</i>, and <i>Scenedesmus rubescens</i> under the conditions of a newly developed photobioreactor is investigated. It is shown that the addition of sludge in concentrations of 0.1–1 g/L stimulates culture growth. The maximum number of <i>C. fusca</i> and <i>C. vulgaris</i> cells is observed at 1 g/L of sludge in the medium, and in <i>S. rubescens</i>, at 0.1 g/L. At the same time, the viability of <i>C. vulgaris</i> decreases by 17%, and <i>S. rubescens</i>, by 23% at the maximum sludge concentration. <i>C. fusca</i> proves to be the most tolerant to its effects. It is worth noting that an increase in the total protein content is noted for <i>C. fusca</i> and <i>S. rubescens</i>, which makes this approach promising for the production of feed additives. The results obtained, taking into account the need to assess environmental safety, can be applied in the development of biotechnologies for the disposal of industrial waste.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 1","pages":"S124 - S134"},"PeriodicalIF":0.8,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}