中华实验和临床病毒学杂志最新文献_第7页

[The treatment effect of immunoglobulin in AIDS with Guillain-Barre syndrome]. 免疫球蛋白治疗艾滋病合并格林-巴利综合征的疗效观察

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Wen-Luo Zhang, Yue-An Cao, Jing Xia, Lu Yang, Wen-Xiu Xie, Yu Wang, Chao-Sheng Peng

引用次数: 0

[Full sequence analysis and characterization of the Shenzhen Norovirus strain SZ2010422]. [深圳诺如病毒株SZ2010422的全序列分析与鉴定]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Cui-Hong Zhang, Jia-Jian Qin, Ya-Qing He, Hai-Long Zhang, Hui-Ying Li, Miao Jin, Ke-Na Chen, Shao-Long Feng, Zhao-Jun Duan

{"title":"[Full sequence analysis and characterization of the Shenzhen Norovirus strain SZ2010422].","authors":"Cui-Hong Zhang, Jia-Jian Qin, Ya-Qing He, Hai-Long Zhang, Hui-Ying Li, Miao Jin, Ke-Na Chen, Shao-Long Feng, Zhao-Jun Duan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To obtain information on viral molecular structural and evolutionary characteristics, we conducted the SZ2010422 full-length genomic analysis.Methods: Primers were designed by New Orleans full sequence, SZ2010422 full genome was amplified by RT-PCR, the whole genome sequence and the capsid domain amino acid sites was analysised after cloned and sequenced.Results: The genome of G II-4 Norovirus SZ2010422 strain was consist of 7559 bp, it revealed three ORFs composites of the whole genome, ORF1 (5100 bp), ORF2 (1623 bp), ORF3 (807 bp) respectively, ORF1 and ORF2 had 19 nucleotide overlap. By evolutionary comparative analysis found SZ2010422 genomic nucleotide sequences with reference strains of G II-4 New Orleans1805 strains the highest homology with a total length of homology was 99.3%, of ORF1 (99.5%), ORF2 (99.2%), ORF3 (98.6%). Phylogenetic analyses showed SZ2010422 belonging to G II-4 New Orleans variant. Date of 541 amino acid analyses showed: New Orleans variant strains of popular sites: aa310N or K, --> S aa341D --> of N, aa359T--> S, aa396H --> P, aa460H --> Y.Conclusion: Norovirus SZ2010422 belonged to the G II-4 New Orleans variant. In This study, SZ2010422 full sequence can be used not only as a full-length NoV variant sequence standard for future comparison studies, but also as useful material for the public health field by enabling the diagnosis, vaccine development, and prediction of new emerging variants. Noroviruses; Genes; Sequence analysis","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"181-3"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31939082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evolutionary and transmission characteristics of rabies viruses in Asia]. [狂犬病病毒在亚洲的进化和传播特征]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Xiao-Yan Tao, Hao Li, Qing Tang, Guo-Dong Liang

引用次数: 0

[Dynamic analysis of the lymphocyte subsets in HCV children with different genotypes during treatment]. [不同基因型HCV患儿治疗期间淋巴细胞亚群动态分析]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Da-Gang Wang, Lin Chen, Yuan-Li Mao, Fen Qu, Hai-Bin Wang, Cui-Li Yao, Jian-Gong Zhu

引用次数: 0

[Cloning and expressing of tissue inhibitor of metalloproteinases I gene fragment and preparation of monoclonal antibodies against the recombinant protein]. 金属蛋白酶组织抑制剂I基因片段的克隆表达及重组蛋白单克隆抗体的制备

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Jun Hou, Jian Zhang, Jing-Xia Guo, Lin Cheng, Jing Zhao, Jia Liu, Jun Xu, Ai-Xia Liu, Yong-Ji Song, Pan-Yong Mao, Bo-An Li, Yuan-Li Mao

{"title":"[Cloning and expressing of tissue inhibitor of metalloproteinases I gene fragment and preparation of monoclonal antibodies against the recombinant protein].","authors":"Jun Hou, Jian Zhang, Jing-Xia Guo, Lin Cheng, Jing Zhao, Jia Liu, Jun Xu, Ai-Xia Liu, Yong-Ji Song, Pan-Yong Mao, Bo-An Li, Yuan-Li Mao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To prepare the monoclonal antibody (mAb) against tissue inhibitor of metalloproteinases I (TIMP-I) fusion protein.Methods: TIMP-I gene was amplified from fibrotic human liver tissue by RT-PCR, then ligated with pQE31 to form recombinant plasmid pQE-TIMP-I and transformed into E. coli BL21. The protein induced by IPTG was purified by 6 x His-tag and used to immunize the BALB/c mice. The specific monoclonal antibodies (mAbs) were prepared by the cell fusion technique. Western Blot were used to detect specificity of mAbs.Results: The prokaryotic plasmid expressing the recombinant protein was constructed, and the TIMP-I recombinant protein was expressed and purified. Four hybridoma cell lines that secreted anti-TIMP-I mAbs were obtained. 3 of 4 mAbs were the IgG1 subtype. Western Blot indicated the mAbs showed specific combination with TIMP-I protein.Conclusion: The TIMP-I recombinant protein is highly purified and has strong antigenicity. The anti- TIMP-I mAbs were prepared successfully.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"231-3"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31940059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Selection pressure analysis of H3N2 influenza virus from China between 1992 and 2012]. [1992 - 2012年中国H3N2流感病毒选择压力分析]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Yu Lan, Xiang Zhao, Xi-Yan Li, Ye Zhang, Jun-Feng Guo, Ming Li, Wei-Juan Huang, He-Jiang Wei, Yan-Hui Cheng, Min-Ju Tan, Zhao Wang, Lei Yang, Ning Xiao, Da-Yan Wang, Yue-Long Shu

引用次数: 0

[A double antibody sandwich ELISA based assay for titration of severe fever with thrombocytopenia syndrome virus]. [基于双抗体夹心ELISA法测定发热伴血小板减少综合征病毒]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Lin Liu, Quan-Fu Zhang, Chuan Li, Jian-Dong Li, Xiao-Lin Jiang, Fu-Shun Zhang, Wei Wu, Mi-Fang Liang, De-Xin Li

{"title":"[A double antibody sandwich ELISA based assay for titration of severe fever with thrombocytopenia syndrome virus].","authors":"Lin Liu, Quan-Fu Zhang, Chuan Li, Jian-Dong Li, Xiao-Lin Jiang, Fu-Shun Zhang, Wei Wu, Mi-Fang Liang, De-Xin Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To develop an assay for titration of severe fever with thrombocytopenia syndrome virus (SFTSV) based on double antibody sandwich ELISA.Methods: A double antibody sandwich ELISA was developed for detection of SFTSV based on SFTSV nucleocapsid (N) protein specific poly- and monoclonal antibodies, procedures were optimized and evaluated. This ELISA based titration assay was compared with fluorescence assasy and plaque assay based titration method.Results: The results suggested that the titers obtained by ELISA based method are consistent with those obtained by IFA based method (R = 0.999) and the plaque assay titration method (R = 0.949).Conclusion: The novel ELISA based titration method with high sensitivity and specificity is easy to manage and perform, and can overcome the subjectivity associated with result determination of the fluorescence assay and plaque assay based methods. The novel ELISA based titration method can also be applied to high throughput detection.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"215-7"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31940054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A novel SELEX method for screening of HIV-1 P24 antigen]. [一种新的SELEX方法筛选HIV-1 P24抗原]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Shao-Bing Zhan, Xiao-Guang Zhang, Hong-Xia Li, Yi Zeng

引用次数: 0

[Development of EV71, CA16 and other enterovirus vrial real-time qualitative PCR diagnostic kit]. EV71、CA16等肠道病毒实时定性PCR诊断试剂盒的研制

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Li-Qin Li, Jing Zhong, Lin-Fu Zhou, Fu-Chu Qian, Jia-Wei Wang, Li-Cheng Dai

{"title":"[Development of EV71, CA16 and other enterovirus vrial real-time qualitative PCR diagnostic kit].","authors":"Li-Qin Li, Jing Zhong, Lin-Fu Zhou, Fu-Chu Qian, Jia-Wei Wang, Li-Cheng Dai","doi":"","DOIUrl":"","url":null,"abstract":"Objective: A novel multiplex real-time RT-PCR kit was developed to detect EV71, CoxA16 and other human enteroviruses simultaneously with an internal amplification control to avoids false negatives, which used for hand, foot and mouth disease in the clinical diagnosis and epidemiological surveillance.Methods: Design specific primers and probes of EV71, CA16, other intestinal virus and internal amplification control, improve the extraction method of virus nucleic acid. Optimization the detection system of real-time quantitative PCR. Research the products of the accuracy, stability, precision, amplification efficiency and detection of linear range.Results: The primers and probes had high spicificity. The Viral RNA extraction effect of this Kit is as same as that of QIAamp Viral RNA mini Kit (QIAGEN company), but less reagent cost. The optimal concentrations of primers and probes are 0.2 micromol/L for all the upstream and downstream primers, 0.06 micromol/L for probes of other human enteroviruse, 0.08 micromol/L for probes of EV71 and CA16 respectively. The kit has good stability, accuracy and precision. The amplification efficiencies of EV71, CoxA16 and other human enteroviruses are 106% ,101% and 105% and the detection of linear range is from 10(9) copies/microl-10(2) copies/microl.Conclusion: The novel multiplex real-time RT-PCR kit for detecting EV71, CoxA16 and other human enteroviruses simultaneously with an internal amplification control has good stability, accuracy, precision and amplification efficiencies. So it has great value in clinical application.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"224-7"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31940057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Identification of Env-specific monoclonal antibodies from Chinese HIV-1 infected person by magnetic beads separating B cells and single cell RT-PCR cloning]. [磁珠分离B细胞和单细胞RT-PCR克隆技术鉴定中国HIV-1感染者env特异性单克隆抗体]。

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Xiang-Ying Huang, Shuang-Qing Yu, Zhan Cheng, Jing-Rong Ye, Ke Xu, Xia Feng, Yi Zeng

{"title":"[Identification of Env-specific monoclonal antibodies from Chinese HIV-1 infected person by magnetic beads separating B cells and single cell RT-PCR cloning].","authors":"Xiang-Ying Huang, Shuang-Qing Yu, Zhan Cheng, Jing-Rong Ye, Ke Xu, Xia Feng, Yi Zeng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish a simple and practical method for screening of Env-specific monoclonal antibodies from HIV-1 infected individuals.Methods: Human B cells were purified by negative sorting from PBMCs and memory B cells were further enriched using anti-CD27 microbeads. Gp120 antigen labbled with biotin was incubated with memory B cells to specifically bind IgG on cells membrane. The memory B cells expressing the Env-specific antibody were harvested by magnetic beads separating, counted and diluted to the level of single cell in each PCR well that loading with catch buffer containing RNase inhibitor to get RNAs. The antibody genes were amplified by single cell RT-PCR and nested PCR, cloned into eukaryotic expression vectors and transfected into 293T cells. The binding activity of recombinant antibodies to Env were tested by ELISA.Results: Three monocolonal Env-specific antibodies were isolated from one HIV-1 infected individual.Conclusion: We can obtain Env-specific antibody by biotin labbled antigen, magnetic beads separating technique coupled with single cell RT-PCR and expression cloning.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 2","pages":"123-5"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31739223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0