中华实验和临床病毒学杂志最新文献_第8页

[Study on PrP106-126 peptide disturbed dimeration of 14-3-3beta]. [PrP106-126肽干扰14-3-3 - β二聚体的研究]。

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Qin-Qin Song, Peng Sun, Juan Song, Lin-Jun Sheng, Jin Zhang, Jun Han

{"title":"[Study on PrP106-126 peptide disturbed dimeration of 14-3-3beta].","authors":"Qin-Qin Song, Peng Sun, Juan Song, Lin-Jun Sheng, Jin Zhang, Jun Han","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate both PrP and PrP106-126 peptide effect on 14-3-3beta dimeration.Methods: 14-3-3beta were incubated with different does recombinant PrP or PrP106-126 peptide, both 14-3-3beta dimer and polymer were separated 15% non-denaturing polyacrylamide gel electrophoresis (PAGE) and the 14-3-3 dimers were evaluated using 14-3-3beta-specific Western blotting. And then,14-3-3beta dimeration buffer were incubated with different does recombinant PrP and 250 micromol/L PrP106-126 peptide, 14-3-3beta dimer and polymer were detected by above methods. Cellular 14-3-3 dimer were also detected after PrP106-126 peptide were added to HeLa cell for 8 hours.Results: Recombinant full-length PrP facilitated the dimerization of 14-3-3beta and PrP106-126 disturbed 14-3-3beta dimeration as both have dose dependence effect. PrP antagonized PrP106-126-induced 14-3-3beta dimer with PrP protein increase in vitro. Cellular 14-3-3 dimerization also decreased after treatment of peptide PrP106-126 on HeLa cells for 8 hours.Conclusion: [corrected] Dimerization process of 14-3-3beta was promoted by full-length PrP (PrP23-231) but inhibited by peptide PrP106-126 in vitro. PrP agonized PrP106-126-induced inhibition of 14-3-3 dimeration. PrP106-126 inhibited cellular 14-3-3 dimerization.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 2","pages":"109-11"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31739802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Dendritic cell subsets and function in newborns from mothers of different HBV infection status]. [不同HBV感染状态母亲的新生儿树突状细胞亚群和功能]。

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Hong-Xiao Hao, Yan-Li Zhang, Ming-Hui Li, Lu-Xue Zhang, Wei Yi, Yu-Hong Hu, Nuo Yi, Jun Cheng, Shun-Ai Liu, Yao Xie

{"title":"[Dendritic cell subsets and function in newborns from mothers of different HBV infection status].","authors":"Hong-Xiao Hao, Yan-Li Zhang, Ming-Hui Li, Lu-Xue Zhang, Wei Yi, Yu-Hong Hu, Nuo Yi, Jun Cheng, Shun-Ai Liu, Yao Xie","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The aim of this study was to explore the frequency of mDC and pDC and expression of surface markers of the neonates and to discuss the effect of different status of HBV infection of mother on biological characteristics of DC.Methods: Umbilicus cord blood in neonates of HBeAg positive HBV infected mother, HBeAg negative HBV infected mother, and normal mother were collected respectively; peripheral blood of healthy adults were selected as control group. Flow cytometry was employed to detect frequency of the mDC and its expression of CD86, frequency of pDC and its expression of CD80, CD83, CD86, and FlowJo software was used to compare these indicators among the groups.Results: Compared with control group, the frequency of mDC of cord blood (0.29 +/- 0.16 vs 0.81 +/- 0.17), CD86 positive rate of mDC (10.72 +/- 10.01 vs 32.13 +/- 7.46), the frequency of pDC (0.15 +/- 0.07 vs 0.30 +/- 0.07), and CD86/CD83 positive rate of pDC (31.61 +/- 12.81 vs 74.96 +/- 9.78; 42.66 +/- 20.83 vs 82.00 +/- 6.94) were lower (t = -7.86, P = 0.00; t = -5.36, P = 0.00; t = -5.43, P = 0.00; t = -8.49. P = 0.00; t = -4.90, P = 0.00).Conclusions: The frequency of mDC and pDC in umbilical cord blood was lower than the peripheral blood of healthy adult, which was the possible mechanism of newborns easier to chronicity after the infection of hepatitis B virus. A significant correlation was found between different status of HBV infection and costimulatory molecule CD86 positive rate of mDC, but not for the frequency of mDC and pDC, and the expression of pDC molecules.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 2","pages":"112-4"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31739803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of neuraminidase gene of influenza virus H1N1 in baculovirus-expression system]. 甲型H1N1流感病毒神经氨酸酶基因在杆状病毒表达系统中的表达

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Li-Hong Yao, Jin-Qi Fu, Ai-Jun Chen, Xiao-Yu Liu, Peng-Wei Xu, Jian-Qiang Guo, Le-Cui Zhang

{"title":"[Expression of neuraminidase gene of influenza virus H1N1 in baculovirus-expression system].","authors":"Li-Hong Yao, Jin-Qi Fu, Ai-Jun Chen, Xiao-Yu Liu, Peng-Wei Xu, Jian-Qiang Guo, Le-Cui Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To construct the recombinant baculovirus with NA gene of Influenza H1N1 virus.Methods: Full-length NA gene of Influenza virus H1N1 (A/PR/8/34) was amplified by PCR and inserted into pFastBacdual vector to construct the recombinant baculovirus transfer vector pFBD-NA. Recombinant shuttle vectors rBacmid-NA was then obtained after transforming DH10B competent cells containing bacmid plasmids. After transfecting into sf9 cells, recombinant baculovirus rBac-NA was obtained. The rBac-NA genome was extracted and identified by PCR. NA protein expressed by recombinant baculovirus-infected sf9 cells was determined by IFA, Western Bolt and ELISA.Results: PCR results proved that recombinant shuttle vectors rBacmid-NA was successfully constructed. NA protein was detected by IFA and showed strong specific green fluorescence on the surface of infected cells. NA protein was recognized by two polyclonal antibodies specific for NA in Western Blot. ELISA showed specific reaction of recombinant NA protein with mouse polyclonal antibody against influenza virus (PR8), indicating high antigenicity.Conclusion: Recombinant baculovirus rBac-NA that expresse NA protein of influenza virus was successfully constructed. This work provides a basis for further study on NA protein function and novel influenza vaccine development.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 2","pages":"81-4"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31739883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Reverse genetics platform construction of influenza pandemic virus strain]. 流感大流行病毒株反向遗传平台的构建

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Hong Bo, Ye Zhang, Wei-Juan Huang, Xiang Zhao, Jun-Feng Guo, Da-Yan Wang, Yue-Long Shu

引用次数: 0

[Detection of human parvovirus B19, human bocavirus and human parvovirus 4 infections in blood samples among 95 patients with liver disease in Nanjing by nested PCR]. [应用巢式PCR检测南京市95例肝病患者血液中人细小病毒B19、人博卡病毒和人细小病毒4感染情况]。

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Rui Tong, Wei-Min Zhou, Xi-Jun Liu, Yue Wang, Yong-Liang Lou, Wen-Jie Tan

引用次数: 0

[The effect of aluminum adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine]. [铝佐剂和免疫方案对沙宾灭活脊髓灰质炎病毒疫苗免疫原性的影响]。

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Fang Wang, Ming Zhang, Bing-Feng Xie, Han Cao, Shao-Yong Tong, Jun-Rong Wang, Xiao-Ping Yu, Yang Tang, Jing-Ran Yang, Ming-Bo Sun

{"title":"[The effect of aluminum adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine].","authors":"Fang Wang, Ming Zhang, Bing-Feng Xie, Han Cao, Shao-Yong Tong, Jun-Rong Wang, Xiao-Ping Yu, Yang Tang, Jing-Ran Yang, Ming-Bo Sun","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the effect of aluminume adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine.Methods: Four batches of Sabin IPV were produced by different concentrations of type 1, 2, and 3 poliovirus and administrated on three-dose schedule at 0, 1, 2 months and 0, 2, 4 months on rats. Serum samples were collected one month after each dose and neutralizing antibody titers against three types poliovirus were determined by micro-neutralization assay.Results: The GMTs of neutralizing antibodies against three types poliovirus increased significantly and the seropositivity rates were 100% in all groups after 3 doses. There was no significant difference between two immunization schedules, and the 0, 2, 4 month schedule could induce higher level neutralizing antibody compared to the 0, 1, 2 month schedule. The groups with aluminum adjuvant could induce higher level neutralizing antibody compared to the groups without adjuvant.Conclusion: Aluminum djuvant and immunization schedule could improve the immunogenicity of Sabin IPV.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 2","pages":"102-4"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31739800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Prokaryotic soluble expression of protein D of Haemophilus influenzae type b]. b型流感嗜血杆菌蛋白D的原核可溶性表达

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Meng-Meng Yin, Qiu-Dong Su, Min-Zhuo Gyo, Yi-Na Cun, Yuan-Qian Pu, Zhi-Yuan Jia, Jing-Ran Yang, Yang Tang, Guo-Yang Liao, Yao Yi, Sheng-Li Bi, Wei-Dong Li

引用次数: 0

[Epidemiological features on 3 important viral diarrhea among children in Zhuhai during winter and spring]. 珠海市冬春季3种重要儿童病毒性腹泻流行病学特征

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Ya-Wei Liu, Wan-Shan Wang, Qiu-Hua Mo, Ze Yang, Tian Du

{"title":"[Epidemiological features on 3 important viral diarrhea among children in Zhuhai during winter and spring].","authors":"Ya-Wei Liu, Wan-Shan Wang, Qiu-Hua Mo, Ze Yang, Tian Du","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore the epidemiological characteristics of viral diarrhea of norovirus (NV), sapovirus (SV) and astrovirus (AstV) among children in Zhuhai during winter and spring.Methods: Stool specimens were collected from children with viral diarrhea in Maternal and Child Health Hospital of Zhuhai from November 21, 2009 to April 3, 2010. Nucleic acid of NV, SV and AstV from negative specimens of rotavirus and adenovirus were detected by using Reverse transcription-polymerase chain reaction (RT-PCR), and the types of positive samples of NV were also classified at the same time.Results: The total detection rate of the three viruses is 21.49 percent, the highest detection rate is 29.05% in December 2009, the lowest detection rate is 12.20% in February 2010, 87.96% of positive specimens were from children patients aged from 0 to 30 months. The season detection rate of NV, SV and AstV are 14.70%, 2.75% and 4.04% respectively. There were significant differences of NV and SV detection rates in every month of the season, whereas the AstV detection rate was comparatively stable. The highest detection rate of NV is 34.09% in children patients aged from 12 to 18 months, the highest SV detection rate is 12.5% in children patients aged from 60 to 120 months, and the highest AstV detection rate is 16.67% in children patients aged from 24 to 30 months. All the NV were belong to G II genogroup.Conclusions: NV is one of the main pathogens causing viral diarrhea among children in Zhuhai during winter and spring, SV and AstV are also important pathogens. So we should strengthen the monitoring of viral diarrhea caused by NV, SV and AstV in infants and young children.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 2","pages":"98-101"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31739799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Establishment and clinical application of TapMan real-time RT-PCR method for detection of HHV-6]. [TapMan实时RT-PCR检测HHV-6方法的建立及临床应用]。

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Qian-Qian Chen, Bing Zhang, Zhi-Ping Xie, Jin-Song Li, Han-Chun Gao, Ni-Guang Xiao, Le-Yun Xie, Tian Yu, Sai-Zhen Zeng, Ping Gong, Zhao-Jun Duan

引用次数: 0

[Virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia]. [122例重症肺炎患儿支气管肺泡灌洗液病毒检测]。

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Le-Yun Xie, Li-Li Zhong, Bing Zhang, Zhao-Jun Duan, Zhi-Ping Xie, Han-Chun Gao, Qian-Qian Chen, Zhong-Hua Deng, Lin Lin, Xiao-Qian Gao, Ni-Guang Xiao

{"title":"[Virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia].","authors":"Le-Yun Xie, Li-Li Zhong, Bing Zhang, Zhao-Jun Duan, Zhi-Ping Xie, Han-Chun Gao, Qian-Qian Chen, Zhong-Hua Deng, Lin Lin, Xiao-Qian Gao, Ni-Guang Xiao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the prevalence of viral pathogen in children with severe pneumonia in Hunan.Method: Bronchoalveolar lavage fluid [BALF] were collected from 122 hospitalized children with severe pneumonia in People's Hospital of Hunan province from January 2011 to December 2011. Nested- or reverse transcription Polymerase chain reaction (PCR or RT-PCR) was used to screen Adenovirus (ADV), Human Bocavirus (HBoV), Parainfluenzaviruses1-4 (PIV1-4), Human Respiratory Syneytial virus (RSV), Influenza virus A (IFVA), Influenza virus B (IFVB), Human Rhinovirus(HRV), Human Metapneumovirus (HMPV), human coronaviruses NL63 and HKU1 (HCoV-NL63, HCoV- HKU1).Results: Among the 122 bronchoalveolar lavage fluid, viral agents were detected in 60 samples(49.1%), among which ADV (40.98%) was the most common virus, followed by RSV (7.37%) and HBoV (7.37%). Two viruses were detected in 21 individual (35%) samples, of which 20 were dual positive for ADV (40%).Conclusion: ADV is the most frequently detected viral etiology of severe pneumonia in children in Hunan during this year. And its Coinfection with other respiratory viruses was common.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 2","pages":"95-7"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31739798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0