中华实验和临床病毒学杂志最新文献_第10页

[The studies on clinical value of AFP-L3 detected by microspincolum method for judgment of treatment response in patients with HCC undergoing TACE]. [微尖柱法检测AFP-L3在肝癌TACE患者治疗反应判断中的临床价值研究]。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Xiang Zhou, Hong-ming Pan, Gong-ying Chen, Meng-fei Zhu, Xiong-ying Zhu, Jie Wang, Bai-lu Ren

引用次数: 0

[Analysis of the human papillomavirus in laryngeal squamous cell carcinoma patients]. 喉鳞状细胞癌患者人乳头瘤病毒的分析

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Wei Wei, Yun-tao Song, Bao-yun Zhang, Jun-yong Sun, Cao Chen, Nai-song Zhang, Xiao-ping Dong, Qi Shi

引用次数: 0

[Detected of associated contagious parameters of blood recipients before transfusion and their clinical significance]. 输血前受血者相关传染病参数检测及其临床意义。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Ming-lei Li, Xiao-xiang Liu, Ai-hua Sun

{"title":"[Detected of associated contagious parameters of blood recipients before transfusion and their clinical significance].","authors":"Ming-lei Li, Xiao-xiang Liu, Ai-hua Sun","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the significance in prevention of nosocomial infection of the testing of the associated contagious parameters of blood recipients before transfusion.Methods: A retrospective analysis was adopted, 44 968 pre-transfusion patients were tested the serum hepatitis B virus surface antigen (HBsAg), antibody against hepatitis C virus (anti-HCV), antibody against T. pallidum (anti-TP) and antibody against human immunodeficiency virus(anti-HIV).Results: The total positive rate was 22.41%. Positive rate of HBsAg, anti-HCV and anti-TP were 20. 67% (9294/44 968) , 0.33% (148/ 44 968) and 1.65% (9741/44968), respectively; anti-HIV was positive in 39 patients, 23 cases coinfection of the other three indicators at least one positive in 39 cases of anti-HIV-positive blood recipients, of which was mostly observed T. pallidum; co-infection of HBV, HCV and/or TP were 117 cases, and were mostly observed between HBV and HCV, HCV and TP; for HBV infection the department of digestive medicine was prevalent(Chi2>or=83.0, P <0.01).Conclusion: Part of blood recipients before admission had been infected with a contagious disease. The testing of the associated contagious parameters of blood recipients before transfusion is not only useful for both of the hospital and the patients, but also more important to ensure safe blood transfusion, decrease medial dissatisfaction and to prevent nosocomial infection.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 1","pages":"54-6"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31580606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The preliminary study of HBV genotype distribution and the relationship with clinical manifestation]. HBV基因型分布及其与临床表现关系的初步研究。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Jing Sun, Zhi-Yuan Jia, Jun-Xia Chang, Wei Liu

引用次数: 0

[Analysis of genetic diversity of HIV-1 nef genes from a patient with AIDS dementia complex]. [1例艾滋病痴呆复合体患者HIV-1 nef基因遗传多样性分析]。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Shu-ting He, Yuan-yuan Chi, Shaung-shuang Pu, Jian-wei Liu, Hong-ling Wen, Zhi-yu Wang, Yan-yan Song, Hong-zhi Xu, Li Zhao

{"title":"[Analysis of genetic diversity of HIV-1 nef genes from a patient with AIDS dementia complex].","authors":"Shu-ting He, Yuan-yuan Chi, Shaung-shuang Pu, Jian-wei Liu, Hong-ling Wen, Zhi-yu Wang, Yan-yan Song, Hong-zhi Xu, Li Zhao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the genetic diversity of HIV-1 nef genes from a patient with AIDS dementia complex(ADC) , so as to research the amino acid variability and the pathogenesis of ADC.Methods: The nef gene was amplified with PCR from genomic DNA which was extracted from spleen and different brain tissues(basal ganglia, frontal gray matter, meninges, temporal lobe)of a patient who died of ADC. PCR products were cloned into the pMD19-T vector, after transformation and selection by ampicillin and blue/white spotting. Five of positive clones were sequenced and confirmed with BLAST. HIV-1 nef sequences were processed with BioEdit and MEGA4 to do Neighbor-Joining tree, p-Distances, and values of ds/dn.Results: The samples were all identified as HIV-1 B and genetic variation exists in HIV-1 nef gene isolated from different tissues compared with HXB2. In addition,part of the changes were different between periphery and brain.Conclusion: Variations exist in the HIV-1 nef gene extracted from the ADC patient and the variations from peripheral and central nerve tissues were different,these variations may change the function of Nef,and it needs more research.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 1","pages":"18-21"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31580707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evaluation of specific antibodies of blood receiver sera to Treponema pallidum by chemiluminescence immunoassay]. [化学发光免疫分析法评价血液受体血清对梅毒螺旋体的特异性抗体]。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Xue-chun Wang, Ling-zhi Chen, Qin Yu, Ai-hua Sun

引用次数: 0

[Analysis on genetic characteristics of mumps virus strains circulating in Hunan province in 2011]. 2011年湖南省流行性腮腺炎病毒株遗传特征分析

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Fang-Cai Li, Ai-Li Cui, Hong Zhang, Li Deng, Xing-Yu Xiang, Yi-Wei Huang, Wei-Chao Li, Yun-zhi Liu

引用次数: 0

[Changes and analysis of peripheral white blood cells and lymphocyte subsets for patients with influenza B virus infection]. [乙型流感病毒感染患者外周血白细胞和淋巴细胞亚群的变化及分析]。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Zhen Xu, Jie Sun, Jia-wei Wang, Ye Wu, Feng Yu

{"title":"[Changes and analysis of peripheral white blood cells and lymphocyte subsets for patients with influenza B virus infection].","authors":"Zhen Xu, Jie Sun, Jia-wei Wang, Ye Wu, Feng Yu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the characters and changes of peripheral white blood cells and lymphocyte subsets of patients with influenza B virus infection and to provide evidences for diagnosis, treatment and prognosis of influenza B virus infection.Methods: Peripheral white blood cell parameters and the percentages of lymphocyte subsets in acute and recovery phases of 47 cases of influenza B virus infection patients(32 mild cases and 15 severe cases)were investigated and analyzed,and compared respectively with those of 38 cases of healthy people using whole blood cell analysis and flow cytometry.Results: Peripheral white blood cell counts of mild cases decreased greatly but peripheral white blood cell counts and the neutrophils of severe cases increased significantly in acute phase; the peripheral lymphocytes, CD3, CD4, CD8 and CD19 of all patients with influenza B virus infection decreased greatly in acute phase and quickly recovered in recovery phase;there were similar characteristics of change in NK cells between patients with influenza B virus and healthy people. NK cells absolute counts of severe cases decreased significantly in acute phase.Conclusion: Peripheral white blood cell counts and the neutrophils of increased significantly in acute phase and the great decrease of NK cells absolute counts of patients with influenza B virus infection may suggest the severe tendency of diseases.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 1","pages":"32-4"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31580713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A protein array based on quantum dots (QDs) encoded microbeads for detection of hepatitis C virus]. [基于量子点(QDs)编码微珠的蛋白质阵列用于检测丙型肝炎病毒]。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Jun Liu, Guo-Xiang Zhang

{"title":"[A protein array based on quantum dots (QDs) encoded microbeads for detection of hepatitis C virus].","authors":"Jun Liu, Guo-Xiang Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish a hepatitis C virus (HCV) diagnostic assay using a protein array based on the quantum dots (QDs) encoded microbeads.Method: Using QDs encoded microbeads array and immunofluorescence techniques, the highly purified HCV NS3, NS4, NS5 and Core protein were respectively immobilized on the surface of encoded beads, which were used for the detection of anti-HCV antibody in serum. To evaluate the microbeads protein array, 120 HCV positive and 50 HCV negative samples were tested, and compared with recombinant immunoblot assay(RIBA) results as golden standard. The sensitivity, specificity and accuracy value were calculated.Results: Compared 120 positive samples detected with RIBA, the sensitivity of microbeads array is 97.50% (117/120), the specificity is 96.0% (48/50), and accuracy is 97. 06% [(117 + 48)/(120 + 50)], The sensitivity of microbeads protein array is similar with RIBA methods. In the 120 positive samples tested with protein array, the positive rate of anti-HCV Core is 92. 50% (111/120) , the positive rate of anti-HCV NS3 is 89. 17% (107/120), the positive rate of anti-HCV NS4 is 70. 83% (85/120), the positive rate of anti-HCV NS5 is 52.50% (63/120).","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 1","pages":"67-9"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31580549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Oxidative stress in chronic hepatitis C patients]. [慢性丙型肝炎患者的氧化应激]。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01

Jing Zhao, Yu-chen Fan, Feng Zhang, Yang Yang, Ze-hua Zhao, Feng-kai Sun, Kai Wang

{"title":"[Oxidative stress in chronic hepatitis C patients].","authors":"Jing Zhao, Yu-chen Fan, Feng Zhang, Yang Yang, Ze-hua Zhao, Feng-kai Sun, Kai Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: This study is aimed to investigate oxidative stress status in chronic hepatitis C (CHC) patients.Methods: 52 CHC patients were divided into two groups according to the serum level of alanine aminotransferase (ALT): group A (elevated ALT group) and group B (normal ALT group). 20 healthy controls were included in this study. Serum levels of xanthine oxidase (XOD), malondialdehyde (MDA), oxidizided glutathione (GSSG), glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), glutathione reductase (GR) and vitamin C (Vc) were determined by enzyme-linked immunosorbent assay (ELISA).Results: Serum levels of XOD, MDA, GST and GR increased in CHC patients compared with healthy controls. While, serum levels of GSH, GSH-Px and Vc decreased compared with healthy controls. Furthermore, serum levels of XOD, MDA, GSSG, GST and GR in group A were up-regulated compared with group B. Serum levels of GSH, GSH-Px and Vc in group A were down-regulated compared with group B. In CHC patients, serum ALT level positively correlated with serum levels of XOD, MDA, GSSG and GST, while, negatively correlated with serum levels of GSH, GSH-Px and Vc. Serum aspartate aminotransferase (AST) level positively correlated with serum levels of XOD, MDA, GSSG, GR and GST, while, negatively correlated with serum GSH-Px level in CHC patients. Serum gamma-glutamyl transpeptidase (GGT) level positively correlated with serum GR level and negatively correlated with serum GSH level in CHC patients. Serum alkaline phosphatase (AKP) level positively correlated with serum levels of MDA and GR in CHC patients. In CHC patients, serum XOD level was positively related with serum HCV RNA level.Conclusion: Oxidative stress was increased in CHC patients. In CHC patients with elevated serum ALT level, oxidative stress usually became serious.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 1","pages":"47-9"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31580604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0