{"title":"[化学发光免疫分析法评价血液受体血清对梅毒螺旋体的特异性抗体]。","authors":"Xue-chun Wang, Ling-zhi Chen, Qin Yu, Ai-hua Sun","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to evaluate the diagnostic performance of chemiluminescence immunoassay (CLIA ) , in comparison with that of the following currently used treponemal tests: hemagglutination test (TPHA), and Western Blot (WB).</p><p><strong>Methods: </strong>First, a retrospective study was performed with a panel of 18 494 blood receiver sera by CLIA and TPHA, the specific antibody against T. pallidum in 177 were positive sera by CLIA and/or TPHA, 81 clinical and serologically characterized syphilitic sera, 55 sera obtained from subjects with potentially interfering diseases, and 250 healthy sera were negative were detected by CLIA, TPHA and WB.</p><p><strong>Results: </strong>The results of WB as the gold standard, the sensitivity of CLIA (98. 4%) was significantly higher than that of TPHA (94. 4%) (Chi2 = 5.76,P <0. 05), the specificity of CLIA (100%) was similar to that of TPHA (99.7%) (Chi2 =1. 0, P >0. 05).</p><p><strong>Conclusion: </strong>CLIA is characterized with higher sensitivity and specificity, It is suitable for screening Syphilis in clinical laboratory.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 1","pages":"70-2"},"PeriodicalIF":0.0000,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Evaluation of specific antibodies of blood receiver sera to Treponema pallidum by chemiluminescence immunoassay].\",\"authors\":\"Xue-chun Wang, Ling-zhi Chen, Qin Yu, Ai-hua Sun\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The purpose of this study was to evaluate the diagnostic performance of chemiluminescence immunoassay (CLIA ) , in comparison with that of the following currently used treponemal tests: hemagglutination test (TPHA), and Western Blot (WB).</p><p><strong>Methods: </strong>First, a retrospective study was performed with a panel of 18 494 blood receiver sera by CLIA and TPHA, the specific antibody against T. pallidum in 177 were positive sera by CLIA and/or TPHA, 81 clinical and serologically characterized syphilitic sera, 55 sera obtained from subjects with potentially interfering diseases, and 250 healthy sera were negative were detected by CLIA, TPHA and WB.</p><p><strong>Results: </strong>The results of WB as the gold standard, the sensitivity of CLIA (98. 4%) was significantly higher than that of TPHA (94. 4%) (Chi2 = 5.76,P <0. 05), the specificity of CLIA (100%) was similar to that of TPHA (99.7%) (Chi2 =1. 0, P >0. 05).</p><p><strong>Conclusion: </strong>CLIA is characterized with higher sensitivity and specificity, It is suitable for screening Syphilis in clinical laboratory.</p>\",\"PeriodicalId\":70973,\"journal\":{\"name\":\"中华实验和临床病毒学杂志\",\"volume\":\"27 1\",\"pages\":\"70-2\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验和临床病毒学杂志\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验和临床病毒学杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Evaluation of specific antibodies of blood receiver sera to Treponema pallidum by chemiluminescence immunoassay].
Objective: The purpose of this study was to evaluate the diagnostic performance of chemiluminescence immunoassay (CLIA ) , in comparison with that of the following currently used treponemal tests: hemagglutination test (TPHA), and Western Blot (WB).
Methods: First, a retrospective study was performed with a panel of 18 494 blood receiver sera by CLIA and TPHA, the specific antibody against T. pallidum in 177 were positive sera by CLIA and/or TPHA, 81 clinical and serologically characterized syphilitic sera, 55 sera obtained from subjects with potentially interfering diseases, and 250 healthy sera were negative were detected by CLIA, TPHA and WB.
Results: The results of WB as the gold standard, the sensitivity of CLIA (98. 4%) was significantly higher than that of TPHA (94. 4%) (Chi2 = 5.76,P <0. 05), the specificity of CLIA (100%) was similar to that of TPHA (99.7%) (Chi2 =1. 0, P >0. 05).
Conclusion: CLIA is characterized with higher sensitivity and specificity, It is suitable for screening Syphilis in clinical laboratory.