[化学发光免疫分析法评价血液受体血清对梅毒螺旋体的特异性抗体]。

中华实验和临床病毒学杂志 Pub Date : 2013-02-01
Xue-chun Wang, Ling-zhi Chen, Qin Yu, Ai-hua Sun
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引用次数: 0

摘要

目的:本研究的目的是评价化学发光免疫分析法(CLIA)的诊断性能,并与目前使用的螺旋体试验:血凝试验(TPHA)和免疫印迹(WB)进行比较。方法:首先对18 494份经CLIA和TPHA检测的血液受体血清进行回顾性研究,其中CLIA和/或TPHA检测阳性血清177份,临床和血清学特征的梅毒血清81份,潜在干扰性疾病患者血清55份,CLIA、TPHA和WB检测阴性的健康血清250份。结果:以WB为金标准,CLIA的灵敏度为98。4%)显著高于TPHA(94。4%) (ch2 = 5.76,P < 0.05)。05)。结论:CLIA具有较高的敏感性和特异性,适用于临床实验室梅毒筛查。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Evaluation of specific antibodies of blood receiver sera to Treponema pallidum by chemiluminescence immunoassay].

Objective: The purpose of this study was to evaluate the diagnostic performance of chemiluminescence immunoassay (CLIA ) , in comparison with that of the following currently used treponemal tests: hemagglutination test (TPHA), and Western Blot (WB).

Methods: First, a retrospective study was performed with a panel of 18 494 blood receiver sera by CLIA and TPHA, the specific antibody against T. pallidum in 177 were positive sera by CLIA and/or TPHA, 81 clinical and serologically characterized syphilitic sera, 55 sera obtained from subjects with potentially interfering diseases, and 250 healthy sera were negative were detected by CLIA, TPHA and WB.

Results: The results of WB as the gold standard, the sensitivity of CLIA (98. 4%) was significantly higher than that of TPHA (94. 4%) (Chi2 = 5.76,P <0. 05), the specificity of CLIA (100%) was similar to that of TPHA (99.7%) (Chi2 =1. 0, P >0. 05).

Conclusion: CLIA is characterized with higher sensitivity and specificity, It is suitable for screening Syphilis in clinical laboratory.

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CiteScore
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