[A protein array based on quantum dots (QDs) encoded microbeads for detection of hepatitis C virus].

中华实验和临床病毒学杂志 Pub Date : 2013-02-01
Jun Liu, Guo-Xiang Zhang
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引用次数: 0

Abstract

Objective: To establish a hepatitis C virus (HCV) diagnostic assay using a protein array based on the quantum dots (QDs) encoded microbeads.

Method: Using QDs encoded microbeads array and immunofluorescence techniques, the highly purified HCV NS3, NS4, NS5 and Core protein were respectively immobilized on the surface of encoded beads, which were used for the detection of anti-HCV antibody in serum. To evaluate the microbeads protein array, 120 HCV positive and 50 HCV negative samples were tested, and compared with recombinant immunoblot assay(RIBA) results as golden standard. The sensitivity, specificity and accuracy value were calculated.

Results: Compared 120 positive samples detected with RIBA, the sensitivity of microbeads array is 97.50% (117/120), the specificity is 96.0% (48/50), and accuracy is 97. 06% [(117 + 48)/(120 + 50)], The sensitivity of microbeads protein array is similar with RIBA methods. In the 120 positive samples tested with protein array, the positive rate of anti-HCV Core is 92. 50% (111/120) , the positive rate of anti-HCV NS3 is 89. 17% (107/120), the positive rate of anti-HCV NS4 is 70. 83% (85/120), the positive rate of anti-HCV NS5 is 52.50% (63/120).

[基于量子点(QDs)编码微珠的蛋白质阵列用于检测丙型肝炎病毒]。
目的:建立基于量子点编码微珠的丙型肝炎病毒(HCV)蛋白阵列诊断方法。方法:采用量子点编码微球阵列和免疫荧光技术,将高纯度的HCV NS3、NS4、NS5和Core蛋白分别固定在编码微球表面,用于血清中抗HCV抗体的检测。为了评估微珠蛋白阵列,我们检测了120例HCV阳性和50例HCV阴性样本,并将重组免疫印迹(RIBA)结果作为金标准进行了比较。计算灵敏度、特异度和准确度值。结果:对比120份RIBA检测的阳性样本,微珠阵列检测的灵敏度为97.50%(117/120),特异性为96.0%(48/50),准确度为97。06%[(117 + 48)/(120 + 50)],微珠蛋白阵列的灵敏度与RIBA方法相近。蛋白阵列检测120例阳性样本,抗- hcv Core阳性率为92。50%(111/120),抗- hcv NS3阳性率为89。17%(107/120),抗- hcv NS4阳性率为70。83%(85/120),抗hcv NS5阳性率为52.50%(63/120)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
0.20
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