[Virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia].

中华实验和临床病毒学杂志 Pub Date : 2013-04-01

Le-Yun Xie, Li-Li Zhong, Bing Zhang, Zhao-Jun Duan, Zhi-Ping Xie, Han-Chun Gao, Qian-Qian Chen, Zhong-Hua Deng, Lin Lin, Xiao-Qian Gao, Ni-Guang Xiao

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引用次数: 0

Abstract

Objective: To investigate the prevalence of viral pathogen in children with severe pneumonia in Hunan.

Method: Bronchoalveolar lavage fluid [BALF] were collected from 122 hospitalized children with severe pneumonia in People's Hospital of Hunan province from January 2011 to December 2011. Nested- or reverse transcription Polymerase chain reaction (PCR or RT-PCR) was used to screen Adenovirus (ADV), Human Bocavirus (HBoV), Parainfluenzaviruses1-4 (PIV1-4), Human Respiratory Syneytial virus (RSV), Influenza virus A (IFVA), Influenza virus B (IFVB), Human Rhinovirus(HRV), Human Metapneumovirus (HMPV), human coronaviruses NL63 and HKU1 (HCoV-NL63, HCoV- HKU1).

Results: Among the 122 bronchoalveolar lavage fluid, viral agents were detected in 60 samples(49.1%), among which ADV (40.98%) was the most common virus, followed by RSV (7.37%) and HBoV (7.37%). Two viruses were detected in 21 individual (35%) samples, of which 20 were dual positive for ADV (40%).

Conclusion: ADV is the most frequently detected viral etiology of severe pneumonia in children in Hunan during this year. And its Coinfection with other respiratory viruses was common.

本刊更多论文

[122例重症肺炎患儿支气管肺泡灌洗液病毒检测]。

目的:了解湖南省重症肺炎患儿病毒性病原体的流行情况。方法:收集2011年1月~ 12月湖南省人民医院收治的122例重症肺炎患儿支气管肺泡灌洗液(BALF)。采用巢式或反转录聚合酶链反应(PCR或RT-PCR)筛选腺病毒(ADV)、人bocavv (HBoV)、副流感病毒1-4 (PIV1-4)、人呼吸道合胞病毒(RSV)、流感病毒A (IFVA)、流感病毒B (IFVB)、人鼻病毒(HRV)、人偏肺病毒(HMPV)、人冠状病毒NL63和HKU1 (HCoV-NL63、HCoV- HKU1)。结果:122份支气管肺泡灌洗液中检出病毒因子60份(49.1%)，其中以ADV(40.98%)最为常见，其次为RSV(7.37%)和HBoV(7.37%)。在21份(35%)样本中检测到两种病毒，其中20份ADV双阳性(40%)。结论:ADV是湖南省今年儿童重症肺炎中检出最多的病毒性病因。其与其他呼吸道病毒的合并感染也很常见。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中华实验和临床病毒学杂志

CiteScore

0.20

自引率

0.00%

发文量

4549