中华实验和临床病毒学杂志最新文献_第6页

[The expression of interferon-lambda1 in CHO cell]. [干扰素-lambda1 在 CHO 细胞中的表达]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Wu-Mei Yuan, Fen-Lian Ma, Qian Zhang, Wen-Zhi Zheng, Li-Shu Zheng

{"title":"[The expression of interferon-lambda1 in CHO cell].","authors":"Wu-Mei Yuan, Fen-Lian Ma, Qian Zhang, Wen-Zhi Zheng, Li-Shu Zheng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To construct the eukaryotic expression vector PCI-dhfr-lambda1 and PCI-dhfr-SP163-lambda1 which linked the enhancer SP163 with interferon lambda1. Then express the interferon lambda1 in CHO (dhfr-) cells.Methods: Using PCR method to introduce the restriction enzyme sites and through the fusion PCR binding the enhancer with the interferon Lambda1. After sequenced, lambda1 and SP163-lambda1 was inserted into PCI-dhfr forming the expression vector PCI-dhfr-lambda1 and PCI-dhfr-SP163-lambda1 which was constructed successfully confirming by sequencing. Then the expressing vectors were transfected into CHO (dhfr-) cells using liposome transfection method and interferon lambda1 protein was assayed with indirect immunofluorescence and Western Blot. Using cytopathic effect inhibition evaluated the antiviral activity of interferon lambda1.Results: Successfully constructing the eukaryotic expression vectors of interferon lambda and the vectors could express interferon lambda1. The result of immunofluorescence showed the enhancer developed the expression of interferon lambda1. Detecting the interferon lambda1 in CHO (dhfr-) cells after transfecting 48 hour using Western Blot. The cytopathic effect inhibition showed the expressed interferon lambda1 has the antiviral activity.Conclusion: Successfully expressed the interferon lambda1 in CHO (dhfr-) cells and the protein possesses antiviral activity, which may supply a valuable basis for building the stable cell line of interferon lambda1.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"190-2"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31939085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages]. [发热伴血小板减少综合征病毒在巨噬细胞中的亚细胞定位]

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Ying Han, Yi-Xiang Li, Cong Jin, Chuan Li, Jing Qu, Fu-Shun Zhang, Quan-Fu Zhang, Mi-Fang Liang, Pei-Hong Qiu

引用次数: 0

[The therapeutic effects of pegasys in chronic hepatitis B patients with low-level ALT]. [pegasys对慢性乙型肝炎低ALT患者的治疗效果]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Yun-Song Qian, Ling Li

{"title":"[The therapeutic effects of pegasys in chronic hepatitis B patients with low-level ALT].","authors":"Yun-Song Qian, Ling Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To observe the therapeutic effects of Pegasys (pegylated interferon alpha-2a) in chronic hepatitis B (CHB) patients with low-level alanine transaminase (ALT) < 2 x upper limit of normal (ULN).Methods: One hundred and seven CHB patients were randomized enrolled including 52 with ALT < 2 x ULN and liver tissues inflammation activity > or = G2 as observational group and 55 with ALT > 2 x ULN as control group. All the enrolled patients received pegasys treatment for 48 weeks and the responses between two groups were compared. Measurement data were analyzed using t test and numeration data were analyzed using chi square test.Results: The reductions of HBV DNA in observational group at different time points were all less than control group (all P < 0.05). At the end of treatment, the HBV DNA negative rate, HBeAg seroconversion rate and HBsAg loss rate in the observational group were 51.9%, 48.8% and 1.9% , respectively, which were all lower than control group (67.3% , 66.7% and 7. 3% , respectivley) ( all P <0. 05). The ALT normalization rates of two groups were 75% and 76.4% (P > 0. 05).Conclusion: Pegasys is efficient for CHB patients with ALT < 2 x ULN and liver tissues inflammation activity > or = G2, while it is still inferior to those with ALT > 2 x ULN.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"196-8"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31938466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[To explore the safety testing evaluation index of breast-feeding by hepatitis B-positive mothers]. 探讨乙型肝炎阳性母亲母乳喂养安全性检测评价指标。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Wen-Ping Zhang, He-Ying Shi, Cai-Bin Li, Qi-Hong Li, Peng Du

引用次数: 0

[Comparison of ELISA diagnostic kits used in China for hepatitis C virus IgG antibody detection]. [国内丙型肝炎病毒IgG抗体ELISA检测试剂盒比较]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Qing-Ling Meng, Jian Lu, Feng Qiu, Hai-Yan Shi, Yong-Zhen Jiang, Hong-Lan Zhao, Feng Wang

{"title":"[Comparison of ELISA diagnostic kits used in China for hepatitis C virus IgG antibody detection].","authors":"Qing-Ling Meng, Jian Lu, Feng Qiu, Hai-Yan Shi, Yong-Zhen Jiang, Hong-Lan Zhao, Feng Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To compare the commercial diagnostic reagent available in China for hepatitis C virus ( HCV) IgG antibody detection in order to provide some useful information for HCV prevention.Methods: The HCV-IgG detection reagents produced by six different Enterprises named A, B, C, D, E and F were chosen and applied to detect 160 HCV specious sera samples. HCV-IgG reagent from ABBOTT was adopted as gold-standard and the samples in gray zone were determined by RIBA method finally.Results: 160 sera samples comprised 88 positive samples and 72 negative samples. The total conformity ranged from 88.13% to 98.13% and the Youden indexes ranged from 0.74 to 0.96 when the reagents from six different Enterprises were compared with gold-standard. The highest conformity was 98.13%, observed in D reagent with the highest Youden index of 0.96.Conclusion: The total conformity rates were more than 88% when the HCV-IgG antibody detection reagents from six different Enterprises were compared with ABBOTT reagent. It was highly conformable. However, some reagent proved to be less conformable in negative samples detection.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"221-3"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31940056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Construction and characterization of EGFP reporter gene labeled Sindbis virus]. [Sindbis病毒EGFP报告基因的构建与表征]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Ling-Ling Deng, Jiang-Jiao Li, Yan Wei, Huan-Qin Wang, Feng-Juan Zhang, Ji-Guo Sun, Chang Chen, Wu-Yang Zhu, Guo-Dong Liang

引用次数: 0

[Value of high risk human papilloma virus detection in screening and diagnosing cervical lesion in littoral of Zhejiang province]. [高危人乳头瘤病毒检测在浙江省沿海地区宫颈病变筛查诊断中的价值]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Jun Zhu, Ying Lin, Hai-Bo Ruan, Xia-Hui Zhang, Qian Yang

{"title":"[Value of high risk human papilloma virus detection in screening and diagnosing cervical lesion in littoral of Zhejiang province].","authors":"Jun Zhu, Ying Lin, Hai-Bo Ruan, Xia-Hui Zhang, Qian Yang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To analyse the infection of high-risk human papiliomavirus (HR-HPV) in cervical lesion wome, and evaluate the significance of high-risk human pappilomavirus detection by hybrid capture II (HV-II) in screening and diagnosing cervical lesion, especially high grade cervical intraepithelial neoplasia (CIN).Methods: A series of 1130 patients of cervical lesion were preliminarily diagnosed by cervical cytological examination, HR-HPV detection by HC-II , colposcopy and biopsy under the colposcopy between June 2009 and December 2008, including 212 CIN I and (or) condyloma (CIN I/HPV I), 442 CIN II/III, 28 invasive cervical cancer. cervical cytological examination is by thin prep liquid-based cytology test(TCT),and HR-HPV detection is by HC-II.Results: In 1130 cases the positive of HR-HPV was 65.84% (744/1130). Unusual cytology result were 862 cases, with 356 ASCUS, 84 ASCH, 216 LSIL, 184HSIL and 22 cancer. The number of biopsy > or = CINI/HPVI was 682, positive rate of HR-HPV was 78.59% (536/682). In screening CIN II or above, sensitivity, specificity, PPV and NPV of TCT were 88.94%, 32.73%, 48.49%, 80.60%, of HR-HPV DNA detectiort by HC-II were 90.21%, 51.82%, 57.14%, 88.14%, and of HR-HPV detection combined with cytology were 97.45%, 22.42%, 47.22%, 92.50%.Conclusion: The infection rate of HR-HPV in cervical lesions is higher in each age group. Infection rate of HR-HPV is ascending with serious degree of cervical lesion. HR-HPV detection by HC- II is an important method in screening cervical lesion. HR-HPV detection is a viable option in the management of women with ASCUS and LSIL of TCT, with higher sensitivity and NPV.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"212-4"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31938472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular and epidemiological study of human adenovirus among infants with diarrhea in Nanjing]. [南京地区婴幼儿腹泻人腺病毒分子及流行病学研究]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Wen-Juan Gao, Xiao-Le Li, Na Liu, Zhao-Jun Duan, Yu Jin

引用次数: 0

[HBV genotype and liver histology effect of peginterferon alpha treatment of HBeAg-position chronic hepatitis B]. [聚乙二醇干扰素α治疗hbeag位点慢性乙型肝炎的HBV基因型和肝脏组织学影响]。

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Chuan-Tong Lu, Guo-Sheng Gao, Hua-Dong Yan, Yao-Ren Hu

{"title":"[HBV genotype and liver histology effect of peginterferon alpha treatment of HBeAg-position chronic hepatitis B].","authors":"Chuan-Tong Lu, Guo-Sheng Gao, Hua-Dong Yan, Yao-Ren Hu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the efficacy of PEG-interferon alpha (PEG-IFN alpha) treatment of HBeAg-positive chronic hepatitis B and HBV genotypes and liver tissues effect of HBeAg seroconversion.Methods: 54 cases confirmed by liver biopsy, genotype clear HBeAg positive chronic hepatitis B (CHB) patients according to body weight, respectively, subcutaneous injection of PEG-IFN-alpha2a 135 microg or 180 microg, or PEG-IFN-alpha2b 50 microg, 80 microg or 100 microg once weekly treatment for 48 weeks and followed for 24 weeks after discontinuation. Statistics of HBeAg seroconvertion, HBV genoty pes and liver histology e antigen seroconversion after the end of treatment.Results: 54 patients were followed up at the end of HBeAg seroconversion rate was 29.63% (16/54). Genotype B patients with HBeAg seroconversion rate was 35.29%, 27.03% higher than the C-type patients, but the difference was not statistically significant (chi2 = 0.382, P = 0.537). Inflammation of the liver activity highter ( > G2) , the degree of fibrosis heavier ( > S1) HBeAg seroconversion rate (50.00% vs. 25.00%, 40.90% vs. 21.88%), but were not statistically significant (chi2 = 1.391, 1.444, P = 0.238, 0.229). Activity of HBV genotype, liver inflammation, liver fibrosis and other factors by multivariate Logistic regression analysis, only liver inflammation activity of the important factors of HBeAg seroconversion.Conclusion: Important factors, liver inflammation activity of PEG-interferon alpha treatment of HBeAg-position chronic hepatitis B patients and HBV genotypes and liver fibrosis may be of little significance.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"193-5"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31938465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Assessment of healthy related quality of life in patients with HBV induced liver cirrhosis]. HBV肝硬化患者健康相关生活质量评价

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Jian-Rong Yue, Hong Xie, Hui Yang, Ling Lin, Xiao-Hui Liu, Gang Duan, Li-Hong Zeng, Li-Ping Zhang

{"title":"[Assessment of healthy related quality of life in patients with HBV induced liver cirrhosis].","authors":"Jian-Rong Yue, Hong Xie, Hui Yang, Ling Lin, Xiao-Hui Liu, Gang Duan, Li-Hong Zeng, Li-Ping Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To assess healthy related quality of life in patients with HBV induced liver cirrhosis.Methods: A total of 103 cases of patients with HBV induced liver cirrhosis were recruited from shenzhen Third People's Hospital during January 2009 to January 2012. The health related quality of life were assess using the most commonly used SF-36 scale questionnaire in all 103 patiemts. Sixty patients were further chosen for health related quality assessment using qualitive research method.Results: The quality of life of chronic hepatitis B patients with cirrhosis Child classification, four score of each group general health, vitality, social functioning, mental health was statistically significant (P < 0.05). Depth interviews found that the disease affects the psychological burden of the social needs of the three major affect quality of life factors.Conclusion: Qualitative research with the SF-36 scale associated to a better understanding of the needs of life of patients with chronic hepatitis B cirrhosis, provide a reference for subsequent targeted medical services.","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"199-201"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31938467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0