{"title":"[Construction and characterization of EGFP reporter gene labeled Sindbis virus].","authors":"Ling-Ling Deng, Jiang-Jiao Li, Yan Wei, Huan-Qin Wang, Feng-Juan Zhang, Ji-Guo Sun, Chang Chen, Wu-Yang Zhu, Guo-Dong Liang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).</p><p><strong>Methods: </strong>The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.</p><p><strong>Results: </strong>We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.</p><p><strong>Conclusion: </strong>The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"228-30"},"PeriodicalIF":0.0000,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验和临床病毒学杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).
Methods: The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.
Results: We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.
Conclusion: The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.
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