[A double antibody sandwich ELISA based assay for titration of severe fever with thrombocytopenia syndrome virus].

中华实验和临床病毒学杂志 Pub Date : 2013-06-01

Lin Liu, Quan-Fu Zhang, Chuan Li, Jian-Dong Li, Xiao-Lin Jiang, Fu-Shun Zhang, Wei Wu, Mi-Fang Liang, De-Xin Li

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引用次数: 0

Abstract

Objective: To develop an assay for titration of severe fever with thrombocytopenia syndrome virus (SFTSV) based on double antibody sandwich ELISA.

Methods: A double antibody sandwich ELISA was developed for detection of SFTSV based on SFTSV nucleocapsid (N) protein specific poly- and monoclonal antibodies, procedures were optimized and evaluated. This ELISA based titration assay was compared with fluorescence assasy and plaque assay based titration method.

Results: The results suggested that the titers obtained by ELISA based method are consistent with those obtained by IFA based method (R = 0.999) and the plaque assay titration method (R = 0.949).

Conclusion: The novel ELISA based titration method with high sensitivity and specificity is easy to manage and perform, and can overcome the subjectivity associated with result determination of the fluorescence assay and plaque assay based methods. The novel ELISA based titration method can also be applied to high throughput detection.

本刊更多论文

[基于双抗体夹心ELISA法测定发热伴血小板减少综合征病毒]。

目的:建立一种基于双抗体夹心ELISA法测定发热伴血小板减少综合征病毒(SFTSV)的方法。方法:建立以SFTSV核衣壳蛋白特异性多克隆和单克隆抗体为基础的双抗体夹心ELISA法检测SFTSV病毒，并对检测方法进行优化和评价。将该方法与荧光法和斑块法进行比较。结果:ELISA法所得滴度与IFA法所得滴度一致(R = 0.999)，与斑块测定法所得滴度一致(R = 0.949)。结论:该方法灵敏度高，特异度高，易于管理和操作，克服了荧光法和斑块法测定结果的主观性。该方法也可用于高通量检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中华实验和临床病毒学杂志

CiteScore

0.20

自引率

0.00%

发文量

4549