Acta histochemica最新文献

{"title":"Corrigendum to “Intracerebral injection of oil cyst content of human craniopharyngioma (oil machinery fluid) as a toxic model in the rat brain” [Acta Histochem. 116(3) (2014) 448–56]","authors":"Martha Lilia Tena-Suck , Ma. Elena Hernández-Campos , Alma Ortiz-Plata , Citlaltepetl Salinas-Lara , Ana Laura Colín-González , Abel Santamaría","doi":"10.1016/j.acthis.2023.152089","DOIUrl":"10.1016/j.acthis.2023.152089","url":null,"abstract":"","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152089"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10334880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional human germinal centers of different sizes in patients diagnosed with lymphadenitis show comparative constant relative volumes of B cells, T cells, follicular dendritic cells, and macrophages","authors":"Constantin Maximilian Schemel ,&nbsp;Patrick Wurzel ,&nbsp;Sonja Scharf ,&nbsp;Hendrik Schäfer ,&nbsp;Sylvia Hartmann ,&nbsp;Ina Koch ,&nbsp;Martin-Leo Hansmann","doi":"10.1016/j.acthis.2023.152075","DOIUrl":"10.1016/j.acthis.2023.152075","url":null,"abstract":"<div><p><span><span><span>Germinal centers (GCs) are some of the most important structures in the human immune system. As such, their cell types and functions have been thoroughly investigated. B cells, </span>T cells<span>, follicular dendritic cells (FDCs), and macrophages have widely been found to typically be aggregated in GCs. However, the amount of space occupied by each of these cell types has yet to be investigated. In this study, we conducted confocal laser-based 3D cell-volume quantification of typical GC cells under reactive conditions in </span></span>lymphadenitis<span><span> and investigated how volume proportions change during GC development. For this investigation, we used anti-CD3 (T cells), anti-CD20 and anti-Pax5 (B cells), anti-CD23 (FDCs), anti-CD68 (macrophages), and DAPI<span> (nuclear staining). We detected average proportions of about 11% CD3, 9% CD20, 6% </span></span>CD23<span>, and 2% CD68 in the largest possible regions of interest within GCs. Interestingly, these values remained steady relatively independent of GC size. The remarkably low B cell proportion can be attributed to technical constraints given the use of the CD20 antibody in 3D. Applying the B cell marker Pax5, we found that about 44% of the volume was occupied by B cells after extrapolating the volume of B </span></span></span>cell nuclei to that of whole B cells. We concluded that Pax5 is more suitable than anti-CD20 for 3D B cell quantification in GCs. The substantial unstained volume in GCs raises the question of whether other cell types fill these open spaces. Our 3D investigation enabled a unique morphological and volumetric evaluation of GC cells that balance their overall volumes in GCs.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152075"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9827136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CellProfiler pipeline analysis of cell migration","authors":"Nur Syamimi Ariffin","doi":"10.1016/j.acthis.2023.152074","DOIUrl":"10.1016/j.acthis.2023.152074","url":null,"abstract":"<div><p><span><span>We demonstrate herein a refined method to evaluate the migration capacity of monolayer cells using the CellProfiler pipeline. We used MDA-MB-231 cells, a triple-negative breast cancer cell line<span>, as a model to perform the wound healing assay and proceeded with the pipeline analysis. In order to see a contrast in our analysis of cell migration, we treated the cells with 10 µM kartogenin for 48 h and compared the result to the control cells treated with 0.1 % </span></span>dimethyl sulfoxide (DMSO). The migration rate of MDA-MB-231 cells could be measured precisely using this method whereby in the presence of 10 µM kartogenin, the cells migrated at 6.3 ± 1.7µmh</span>⁻¹ whilst the vehicle control migrated at 9.1 ± 3.2 µmh⁻¹ (p &lt; 0.05). The small changes in the rate of migration could be significantly differentiated and we believe that this method is accurate in analyzing data of scratch assays as it is of high precision and therefore can be used for high-throughput screenings.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152074"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9829107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination","authors":"Diana I. Romero-Sánchez ,&nbsp;Sonia Vázquez-Santana ,&nbsp;Rafael A. Alonso-Alvarez ,&nbsp;Jorge M. Vázquez-Ramos ,&nbsp;Aurora Lara-Núñez","doi":"10.1016/j.acthis.2023.152092","DOIUrl":"10.1016/j.acthis.2023.152092","url":null,"abstract":"<div><p><span>In maize, immunoprecipitation<span> assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose<span> (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase controlling proliferation (a negative signal that slows down the cell cycle). Cell cycle proteins were analyzed by immunolocalization on longitudinal sections of maize </span></span></span>embryo axis<span> in seven different tissues or zones (with different proliferation or differentiation potential) and in the nucleus of their cells. Results showed a prevalence of these cell cycle proteins on embryo axes from dry seeds, particularly, their accumulation in nuclei of radicle cells. The absence of sugar caused the accumulation of these regulators in different proliferating zones. CyclinD2 abundance was reduced during germination in the presence of sucrose along the embryo axis, while there was an increase at 36 h on glucose. KRP proteins showed a slight increase at 18 h and a decrease at 36 h on both sugars. There was no correlation between cell cycle regulators/DNA co-localization on both sugars. Results suggest glucose induced a specific accumulation of each cell cycle regulator depending on the proliferation zone as well as nuclear localization which may reflect the differential morphogenetic program regarding the proliferation potential in each zone, while sucrose has a mild influence on both cell cycle proteins accumulation during germination. Whenever CycD2s were present in the nucleus, KRPs were absent after treatment with either sugar and at the two imbibition times analyzed, along the different embryo axe zones.</span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152092"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10284982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological analysis of cell cannibalism: An auxiliary tool in the prediction of central giant cell granuloma clinical behavior","authors":"Caio César da Silva Barros ,&nbsp;Luiz Miguel da Rocha Santos ,&nbsp;Mara Luana Batista Severo ,&nbsp;Márcia Cristina da Costa Miguel ,&nbsp;Cristiane Helena Squarize ,&nbsp;Éricka Janine Dantas da Silveira","doi":"10.1016/j.acthis.2023.152091","DOIUrl":"10.1016/j.acthis.2023.152091","url":null,"abstract":"<div><p><span><span>Central giant cell granuloma<span> (CGCG) is a benign jaw lesion with variable clinical behavior. Cell cannibalism is a cellular process associated with aggressiveness and invasion in malignant neoplasms. Here, we morphologically investigated cell cannibalism as an auxiliary method to predict CGCG clinical behavior. Cell cannibalism was quantitatively evaluated in 19 cases of peripheral giant cell granuloma (PGCG), 38 cases of CGCG (non-aggressive and aggressive), and 19 cases of </span></span>giant cell tumor of bone<span> (GCT) stained with hematoxylin<span> and eosin. T-test was performed to assess the differences between the variables analyzed (</span></span></span><em>p</em> ≤ 0.05). Cell cannibalism was identified in 21% of non-aggressive CGCGs and 68.4% of aggressive CGCGs. A significantly higher amount of cannibal multinucleated giant cells (CMGC) was observed in aggressive CGCG compared to PGCG and non-aggressive CGCG (<em>p</em> = 0.042; <em>p</em> = 0.044, respectively). There were no significant differences in the CMGC index between non-aggressive CGCG and PGCG (<em>p</em> = 0.858) and between aggressive CGCG and GCT (<em>p</em><span> = 0.069). CGGC cases that exhibited rapid growth and tooth displacement and/or root resorption had a higher amount of CMGC (</span><em>p</em> = 0.035; <em>p</em> = 0.041, respectively). Cell cannibalism can be identified in CGCG through routine anatomopathological examination. The quantification of CMGC can help to predict the clinical behavior of central giant cell granuloma.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152091"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10194116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 induced changes in the glycosylation pattern in the respiratory tract of Golden Syrian hamsters","authors":"Lea-Adriana Barlang ,&nbsp;Björn-Patrick Mohl ,&nbsp;Claudia Blaurock ,&nbsp;Sophia Harder ,&nbsp;Angele Breithaupt ,&nbsp;Olivia M. Merkel ,&nbsp;Anne Balkema-Buschmann ,&nbsp;Andreas Popp","doi":"10.1016/j.acthis.2023.152077","DOIUrl":"10.1016/j.acthis.2023.152077","url":null,"abstract":"<div><p><span>Even after more than two years of intensive research, not all of the pathophysiological processes of Coronavirus Disease 2019 (COVID-19), induced by </span>severe acute respiratory syndrome coronavirus<span><span><span><span> type 2 (SARS-CoV-2) infection, have been fully elucidated. The initial virus-host interaction at the respiratory epithelium plays a crucial role in the course and progression of the infection, and is highly dependent on the </span>glycosylation<span> pattern of the host cell and of the secreted mucins. Glycans are </span></span>polysaccharides<span><span> that can be attached to proteins and thereby add to their stability and functionality. Lectins are glycan-binding proteins that recognize specific glycan motifs, and lectin </span>histochemistry<span><span> is a suitable tool to visualize and examine glycosylation pattern changes in tissues. In this study we used lectins with different glycan-specificities for the visualization of glycosylation pattern changes in the respiratory tract of SARS-CoV-2 infected Golden Syrian hamsters. While some lectins (LEL, STL) enable the visualization of the damage to alveolar </span>type 1 pneumocytes, other lectins, e.g., GSLI, visualized the loss and subsequent </span></span></span>hyperplasia<span><span> of type 2 pneumocytes. UEAI staining was co-localized with KI67, a proliferation marker. Double staining of lectins LEL, STL and WGA with specific </span>immune cell markers (Iba1, CD68) showed co-localization and the dominant infiltration of monocyte-derived macrophages into infected alveolar tissue. The elucidation of the glycosylation pattern of the respiratory tract cells in uninfected and infected Golden Syrian hamsters revealed physiological and pathological aspects of the disease that may open new possibilities for therapeutic development.</span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152077"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9965484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topiramate promotes osteogenic differentiation through AMPK-dependent phosphorylation of Smad1/5/9","authors":"Kyeong-Min Kim ,&nbsp;Hyo-Eun Son ,&nbsp;Young-Ju Lim ,&nbsp;Won-Gu Jang","doi":"10.1016/j.acthis.2023.152095","DOIUrl":"10.1016/j.acthis.2023.152095","url":null,"abstract":"<div><p><span>Topiramate [2,3:4,5-bis-o-(1-methylethylidene) β-</span><span>D</span><span><span><span>-fructo-pyranose sulfamate<span><span>; TPM] is one of the most used new-generation antiepileptic drugs<span><span>. It has been reported to regulate the differentiation of human bone cells. However, the molecular mechanism of TPM in osteoblast differentiation is not fully elucidated. In the present study, we examined the effect of TPM on osteogenic differentiation of C3H10T1/2, MC3T3-E1, primary mouse calvarial cells, and primary </span>bone marrow stem cells (BMSCs). Primary cells were isolated from mice </span></span>calvaria<span> and bone marrow respectively. Expression of the osteogenic gene was determined by RT-PCR. The osteogenic protein levels were measured by Western blot analysis. </span></span></span>Alkaline phosphatase<span><span><span> (ALP) staining experiment was performed to evaluate ALP activity. Alizarin red s (ARS) staining was performed to measure zebrafish </span>caudal fin<span> regeneration. Treatment of TPM up-regulated the osteogenic genes including distal-less </span></span>homeobox 5 (Dlx5) and runt-related transcription factor 2 (Runx2). In addition, TPM also increased the Dlx5 and Runx2 protein levels, Smad1/5/9 phosphorylation, and alkaline phosphatase (ALP) activity. Furthermore, TPM activated </span></span>AMPK, and inhibition of AMPK decreased TPM-induced osteogenic differentiation. In the zebrafish model, osteogenic effect of TPM was identified. TPM was increased amputated caudal fin rays of zebrafish. These results demonstrate that TPM enhances osteogenic differentiation via AMPK-mediated Smad1/5/9 phosphorylation.</span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152095"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41104036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Nur77-TLR4/MyD88 signaling pathway is required for Ginsenoside Rc ameliorates hepatic fibrosis regression by deactivating hepatic stellate cells","authors":"Bo-Feng Qin ,&nbsp;Shan Gao ,&nbsp;Qi-yuan Feng,&nbsp;Wei Chen,&nbsp;Hai-Ming Sun,&nbsp;Jian Song","doi":"10.1016/j.acthis.2023.152079","DOIUrl":"10.1016/j.acthis.2023.152079","url":null,"abstract":"<div><p><span><span>HSCs (hepatic stellate cells) contribute to the excessive extracellular matrix<span> (ECM) deposition plays a key role in the progression of hepatic fibrosis<span>. The present study focused on the hepatoprotective effect of Ginsenoside Rc (Rc), one of the </span></span></span>protopanaxadiol<span><span><span> type ginsenoside, which has contributed to reverse activated HSCs to improve hepatic fibrosis via regulating Nur77-TLR4/MyD88 signaling pathway. We established the hepatic fibrosis model by </span>intraperitoneal injection of </span>carbon tetrachloride (CCl</span></span>₄<span><span>). And HSCs were stimulated with TGF-β, followed by silencing of Nur77, and then incubated in Rc. Rc significantly alleviated histopathological changes, reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Rc could upregulate the Nur77 and downregulate fibrosis markers in the liver of mice, including decreasing the expressions of α-SMA, Collagen-I, the ratio of TIMP-1/MMP-13. Rc significantly increased the expression of Nur77 and suppressed the production of ECM in HSCs. Rc inhibited </span>TLR4<span><span><span><span> signaling pathway, consequently reversing the inflammatory response, including the production of MyD88, </span>IRAK1, </span>IRAK4 and IL-23. When Nur77 was knocked in TGF-β-stimulated HSCs, TLR4 and α-SMA production were increased. Rc suppressed these activatory effects in Nur77 knockdown HSCs. Rc reduced inflammatory reaction by regulating the Nur77-TLR4 signaling pathway while suppressing the </span>fibrogenesis<span> suggesting, underscoring a promising approach of Rc for the treatment in hepatic fibrosis. Targeting Nur77-TLR4 signaling in HSCs would be the potential strategy for Rc against hepatic fibrosis.</span></span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152079"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10277653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A newly improved method of primary cell culture: Tissue block with continuous adhesion subculture in skin fibroblast","authors":"Qiyan Deng ,&nbsp;Lumei Liu ,&nbsp;Ran Tang ,&nbsp;Dehai Xian ,&nbsp;Jianqiao Zhong","doi":"10.1016/j.acthis.2023.152090","DOIUrl":"10.1016/j.acthis.2023.152090","url":null,"abstract":"<div><h3>Background</h3><p>Fibroblasts (FBs) have been widely used as a typical in vitro cell model for investigating the biological processes and cell pathophysiological mechanisms. However, FBs are prone to senescence in cell culture process after several passages. Thus, a new approach to cell culture is quite required to enhance the viability of cells.</p></div><div><h3>Objective</h3><p>To explore a novel method of cell culture based on skin FBs.</p></div><div><h3>Methods</h3><p>Dermal tissue blocks were obtained from BALB/c neonatal mice and randomly divided into experimental group and control group. The experimental group received the newly improved culture method, namely, continuous adherence subculture of tissue block (CASTB) method; while the traditional subculture method was applied in the control group. Cells at 1st, 5th and 10th passages were collected and identified by using histological/immunohistochemical and western blot analysis. Cellular viability, proliferation, senescence and apoptosis were analyzed through application of cell growth curve, CCK-8 assay, Ki67 assay, PCNA protein analysis, β-galactosidase staining, flow cytometry and western blot analysis.</p></div><div><h3>Results</h3><p>Cells under two culture patterns exhibited spindle/irregular shape and vimentin positive expression. With the increase of passage times, the cellular growth rate in the control group gradually decreased, but no alterations emerged from the experimental group. CASTB method remarkably promoted cell growth and proliferation. Moreover, a greatly lower apoptosis and senescence tendency appeared in the experimental group than the control group with passages increasing.</p></div><div><h3>Conclusion</h3><p>The method of CASTB is superior to traditional subculture, offering a large number of primary FBs with higher efficiency and success rate and being worth of further popularization and application.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152090"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10113203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial polarity-driven membrane separation but not cavitation regulates lumen formation of rat eccrine sweat glands","authors":"Zixiu Chen ,&nbsp;Junhong Zhao ,&nbsp;Cangyu Wang ,&nbsp;Xiang Liu ,&nbsp;Zihua Chen ,&nbsp;Jianda Zhou ,&nbsp;Lei Zhang ,&nbsp;Cuiping Zhang ,&nbsp;Haihong Li","doi":"10.1016/j.acthis.2023.152093","DOIUrl":"10.1016/j.acthis.2023.152093","url":null,"abstract":"<div><h3>Background</h3><p>Each eccrine sweat gland (ESG) is a single-tubular structure with a central lumen, and the formation of hollow lumen in the initial solid cell mass is a key developmental process. To date, there are no reports on the mechanism of native ESG lumen formation.</p></div><div><h3>Methods</h3><p><span><span><span>To investigate the lumen morphogenesis and the lumen formation mechanisms of Sprague-Dawley (SD) rat ESGs, SD rat hind-footpads at E20.5, P1–P5, P7, P9, P12, </span>P21<span><span><span>, P28 and P56 were obtained. The lumen morphogenesis of ESGs was examined by HE staining and </span>immunofluorescence staining for polarity markers. The possible mechanisms of lumen formation were detected by </span>terminal deoxynucleotidyl transferase dUTP </span></span>nick end labeling (TUNEL) </span>apoptosis assay<span> and autophagy marker LC3B immunofluorescence staining, and further explored by ouabain intervention experiment.</span></p></div><div><h3>Results</h3><p><span><span>In SD rat ESGs, the microlumen was formed at P1, and the small intact lumen with apical-basal polarity appeared at P3. The expression of apical marker F-actin, basal marker Laminin, basolateral marker E-cadherin was consistent with the timing of lumen formation of SD rat ESGs. During rat ESG development, </span>apoptosis and autophagy were not detected. However, inhibition of Na</span>⁺-K⁺-ATPase (NKA) with ouabain resulted in decreased lumen size, although neither the timing of lumen formation nor the expression of polarity proteins was altered.</p></div><div><h3>Conclusions</h3><p>Epithelial polarity-driven membrane separation but not cavitation regulates lumen formation of SD rat ESGs. NKA-regulated fluid accumulation drives lumen expansion.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"125 7","pages":"Article 152093"},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41094602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}