Acta histochemica最新文献

{"title":"Localization of alpha 2,6-linked sialic acid residues in gastrointestinal tract compartments of some tetrapod’s representatives: Comparative histochemical study","authors":"Aziz Awaad,&nbsp;Ahmed Rushdy,&nbsp;Mohamed A. Adly","doi":"10.1016/j.acthis.2023.152055","DOIUrl":"10.1016/j.acthis.2023.152055","url":null,"abstract":"<div><p><span><span>Epithelial mucins composed mainly of glycoproteins<span><span> and play a vital role as protective barrier against a variety of harmful molecules and microbial infection. Additionally sialic acids, like glycoproteins, are considered as a main component of epithelial mucins and play an important role in </span>mucosal immunity. For example, alpha 2,6-linked galactose/N-acetyl-galactosamine (Gal/GalNAc) sialic acid residues can recognize and mask different biological sites in some intermolecular or intercellular interactions. In this study, the localization sites relationship between general mucins and alpha 2,6-linked Gal/GalNAc sialic acid residues in different compartments in </span></span>gastrointestinal tract<span><span><span> (GIT) of tetrapod representatives were investigated using </span>lectin </span>histochemistry. The toad (</span></span><span><em>Bufo</em><em> regularis</em></span>), lizard (<em>Trachylepis quinquetaeniata</em>), pigeon (<em>Columba livia domestica</em>) and mouse (<span><em>Mus musculus</em></span><span><span><span>) were used as amphibian, reptilian, avian and mammalian representatives respectively. In general, the biodistribution<span> sites of mucins are localized in most compartment sites and partially overlapped with the sites of sialic acid residues in some compartment in each animal representative. Additionally, the localization sites of both mucins and sialic acid in the GIT regions differ based on the tissue type<span> in each tetrapod representative. The mucosa of oesophagus in the toad and lizard showed higher positive signal of general mucins compared with other tetrapod representatives. However, the mucosa of the oesophagus in the toad revealed a positive signal of sialic acid in the tubular glands only, whereas the lizard’s mucosa showed a positive signal of sialic acid in the </span></span></span>goblet cells. Additionally, the pigeon’s oesophagus showed no localization of the sialic acid or mucins while, all layers of the mouse’s oesophagus showed a positive localization of the sialic acid residues. In the stomach, all </span>stomach mucosa<span><span> compartments in all representatives showed positive signal of mucins, while the gastric glands in the toad, pigeon (proventricular glands) and mouse showed signals of sialic acid residues localization but in different trends. While the lizard showed a localization of the sialic acid in the mucosal lamina propria only. Furthermore, the mucosa of the ileum showed positive signal of mucin in the goblet cells and some absorptive cells brush borders in all tetrapod animals. While a higher signal of the sialic acid residues in the absorptive cells but not the goblet cells in the case of the toad and mouse. While the lizard’s ileum showed a higher localization of sialic acid in the goblet cells only. Mucin localization in the rectum was similar to those in ileum. Specifically, the toad and lizard showed signals of the sialic acid residues in","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9637514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dihydroartemisinin inhibited interleukin-18 expression by decreasing YAP1 in hepatocellular carcinoma cells","authors":"Yi Gong ,&nbsp;Qing Peng ,&nbsp;Yuting Gao ,&nbsp;Jiali Yang ,&nbsp;Junlan Lu ,&nbsp;Yuman Zhang ,&nbsp;Yanguang Yang ,&nbsp;Hua Liang ,&nbsp;Yuan Yue ,&nbsp;Xinli Shi","doi":"10.1016/j.acthis.2023.152040","DOIUrl":"10.1016/j.acthis.2023.152040","url":null,"abstract":"<div><h3>Background</h3><p>Yes-associated protein 1<span><span> (YAP1) is highly expressed in liver cancer and has been used as an independent prognostic marker for hepatocellular carcinoma (HCC), while inhibition of YAP1 slows down the progression of HCC. Interleukin-18 (IL-18) also tends to be highly expressed in liver cancer. Previous research has proved that dihydroartemisinin (DHA) plays an important role in HCC </span>treatment by reducing YAP1 expression. However, the relationship between YAP1 and IL-18 has not been reported in HCC, especially during DHA therapy.</span></p></div><div><h3>Objective</h3><p>The purpose of this study was to clarify the relationship between YAP1 and IL-18 in HCC cells, and to explicit the role of IL-18 in the treatment of HCC by DHA.</p></div><div><h3>Methods and results</h3><p><span>We found that YAP1 and IL-18 were highly expressed in patients with hepatocellular carcinoma by bioinformatics analysis. Moreover, </span><em>YAP1</em> was positively correlated with <em>IL18</em> in liver cancer. <em>YAP1</em> and <em>IL18</em><span> correlated with immune cell<span> infiltration, notably T cell exhaustion. </span></span><em>YAP1</em> knockdown decreased IL-18 expression<em>,</em> while <em>YAP1</em> overexpression increased the IL-18 expression in HCC cells. DHA reduced IL-18 expression through YAP1 in HCC cells<em>.</em><span> Further, DHA reduced the growth of Hepa1–6 cells subcutaneous xenograft tumors by inhibiting the expression of YAP1 and IL-18. However, DHA improved IL-18 in serum and adjacent tissues from DEN/TCPOBOP-induced liver tumor model in C57BL/6 mice.</span></p></div><div><h3>Conclusion</h3><p>YAP1 was positively correlated with IL-18 in HCC. DHA reduced the expression of IL-18 by inhibiting YAP1 and plays a role in the treatment of HCC. Our study suggested that IL-18 is a potential target for the treatment of HCC, and DHA is a promising drug for HCC therapy.</p></div><div><h3>Data availability</h3><p>The dataset that supports the findings of this study is available from the corresponding author upon reasonable request.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9522578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical distribution of Ca2+/calmodulin-dependent protein kinase II subunits in the rat carotid body","authors":"Hiroki Saito ,&nbsp;Takuya Yokoyama ,&nbsp;Nobuaki Nakamuta ,&nbsp;Yoshio Yamamoto","doi":"10.1016/j.acthis.2023.152043","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152043","url":null,"abstract":"<div><p><span>Carotid body<span> (CB) activity stimulated by a lower partial oxygen pressure in rats is enhanced by exposure to chronic intermittent hypoxia. However, the mechanisms that modulate CB activity remain unclear. In the present study, the expression and distribution of one of the candidate molecules to modulate reactivity, Ca</span></span>²⁺<span><span><span>/calmodulin-dependent protein kinase II (CaMKII) were examined in the rat CB using reverse transcriptional polymerase chain reaction and </span>immunofluorescence<span> with isoform-specific antibodies. CaMKIIγ and CaMKIIδ were distributed in CB chemoreceptor cells, and exhibited intense </span></span>immunoreactivity<span><span><span> in dopamine β-hydroxylase-positive chemoreceptor cells. CaMKIIβ and CaMKIIγ were distributed in sensory nerve endings attached to chemoreceptor cells of the CB. In the petrosal </span>ganglion, immunoreactivities for CaMKIIα, CaMKIIβ, CaMKIIγ, and CaMKIIδ were detected in the perinuclear region of ganglion cells. The present results indicate that CaMKIIγ and CaMKIIδ in chemoreceptor cells and CaMKIIβ and CaMKIIγ in sensory nerve endings enhanced reciprocal </span>synaptic transmission<span><span>, i.e., noradrenaline and ATP for cells to neurons and </span>glutamate for neurons to cells.</span></span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49769249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KCCs, NKCCs, and NCC: Potential targets for cardiovascular therapeutics? A comprehensive review of cell and region specific expression and function","authors":"Akshat D. Modi ,&nbsp;Areej Naim Khan ,&nbsp;Wing Yan Elizabeth Cheng ,&nbsp;Dharmeshkumar M. Modi","doi":"10.1016/j.acthis.2023.152045","DOIUrl":"10.1016/j.acthis.2023.152045","url":null,"abstract":"<div><p><span><span><span>Cardiovascular diseases, the leading life-threatening conditions, involve cardiac arrhythmia, coronary artery disease, myocardial infarction, heart failure, cardiomyopathy, and heart valve disease that are associated with the altered functioning of cation-chloride </span>cotransporters. The decreased number of cation-chloride cotransporters leads to reduced reactivity to </span>adrenergic stimulation<span><span>. The KCC family is crucial for numerous physiological processes<span> including cell proliferation and invasion, regulation of membrane trafficking, maintaining ionic and osmotic </span></span>homeostasis<span>, erythrocyte swelling, dendritic spine<span> formation, maturation of postsynaptic GABAergic inhibition, and inhibitory/excitatory signaling in neural tracts. KCC2 maintains intracellular chlorine homeostasis and opposes β-adrenergic stimulation-induced Cl- influx to prevent arrhythmogenesis. KCC3-inactivated cardiac tissue shows increased </span></span></span></span>vascular resistance<span><span>, aortic distensibility, heart size and weight (i.e. hypertrophic cardiomyopathy). Due to KCC4’s high affinity for K+, it plays a vital role in cardiac ischemia<span> with increased extracellular K+. The NKCC and NCC families play a vital role in the regulation of saliva volume, establishing the potassium-rich endolymph in the cochlea, sodium uptake in astrocytes, inhibiting </span></span>myogenic response<span><span><span> in microcirculatory beds, regulation of smooth muscle tone<span> in resistance vessels, and blood pressure. NKCC1 regulates chlorine homeostasis and knocking it out impairs cardiomyocyte depolarization and </span></span>cardiac contractility<span><span> as well as impairs depolarization and contractility of </span>vascular smooth muscle rings in the aorta. The activation of NCC in vascular cells promotes the formation of the </span></span>abdominal aortic aneurysm<span>. This narrative review provides a deep insight into the structure and function of KCCs, NKCCs, and NCC in human physiology and cardiac pathobiology. Also, it provides cell-specific (21 cell types) and region-specific (6 regions) expression of KCC1, KCC2, KCC3, KCC4, NKCC1, NKCC2, and NCC in heart.</span></span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geniposide alleviates pressure overload in cardiac fibrosis with suppressed TGF-β1 pathway","authors":"Yanmei Yao ,&nbsp;Leqing Lin ,&nbsp;Wenxue Tang ,&nbsp;Yueliang Shen ,&nbsp;Fayu Chen ,&nbsp;Ning Li","doi":"10.1016/j.acthis.2023.152044","DOIUrl":"10.1016/j.acthis.2023.152044","url":null,"abstract":"<div><h3>Background</h3><p>Cardiac fibrosis is one of the main contributors to the pathogenesis of heart failure. Geniposide (GE), a major iridoid in gardenia fruit extract, has recently been reported to improve skeletal muscle fibrosis through the modulation of inflammation response. This investigation aimed to illuminate the cardio-protective effect and the potential mechanism of GE in cardiac fibrosis.</p></div><div><h3>Material and methods</h3><p>A transverse aortic contraction (TAC) induction mice model was established and GE (0 mg/kg; 10 mg/kg; 20 mg/kg; 40 mg/kg) was administered by oral gavage daily for 4 weeks. Hemodynamic parameters, Masson’s trichrome stain, and hematoxylin-eosin (HE) staining were estimated and cardiomyocyte fibrosis, interstitial collagen levels, and hypertrophic markers were analyzed using qPCR and western blot. <em>In vitro</em>, H9C2 cells were exposed to the Ang II (1 μM) pretreated with GE (0.1 μM, 1 μM, and 10 μM). Cardiomyocyte apoptosis was detected. Moreover, the transforming growth factor β1 (TGF-β1)/Smad2 pathway was assessed in vivo and in vitro.</p></div><div><h3>Results</h3><p>GE significantly ameliorated TAC-induced cardiac hypertrophy, ventricular remodeling, myocardial fibrosis, and improved cardiac function in vivo, and it inhibited Ang II-induced cardiomyocyte apoptosis in vitro<em>.</em> We further observed that the inflammatory channel TGF-β1/Smad2 pathway was suppressed by GE both in vivo and in vitro.</p></div><div><h3>Conclusion</h3><p>These results indicate that GE inhibited myocardial fibrosis and improved hypertrophic cardiomyocytes with attenuated the TGF-β1/Smad2 pathway and proposed to be an important therapeutic of cardiac fibrosis reduced by TAC.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9990340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular alkaline pH enhances migratory behaviors of hepatocellular carcinoma cells as a caution against the indiscriminate application of alkalinizing drug therapy: In vitro microscopic studies","authors":"Nemany A.N. Hanafy","doi":"10.1016/j.acthis.2023.152032","DOIUrl":"10.1016/j.acthis.2023.152032","url":null,"abstract":"<div><p><span><span>The migratory process is a highly organized, differentiated, and polarized stage by which many signaling pathways are regulated to control cell migration. Since the significant evidence of migrating cells is the reorganization of the </span>cytoskeleton<span>. In the recent study, the cell migration model was assessed on the fact that any disruption obtained in the cellular monolayer confluent, may cause stimulation for surrounding cells to migrate. We attempt to demonstrate the morphological alterations associated with these migrating cells. In this case, sterilized 1 N NaOH (1 µl) was used as alkaline burnt. It leads to scratching the monolayer of hepatocellular carcinoma (HLF cell line) allowing cells to lose their connection. Scanning electron microscopy (SEM), fluorescence microscopy, light inverted microscopy, and dark field were used for discovering the morphological alterations associated with migrating </span></span>cancer cells<span>. The findings show that cells exhibited distinctive alterations including a polarizing stage, accumulation of the actin nodules in front of the nucleus, and protrusions. Nuclei appeared as lobulated shapes during migration. Lamellipodia<span> and uropod were extended as well. Additionally, TGFβ1 proved its expression in HLF and SNU449 after their stimulation. It is demonstrated that hepatocellular carcinoma cells<span> can migrate after their stimulation and there is a caution against the indiscriminate application of alkalinizing drug therapy.</span></span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9522579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Cu/Zn-SOD and Mn-SOD on UVC radiation-induced damage in NIH/3T3 cells and murine skin","authors":"Disi Chen ,&nbsp;Xiaoyang Ai ,&nbsp;Yang Li ,&nbsp;Yue Li ,&nbsp;Yunfan Ao ,&nbsp;Jun Rong ,&nbsp;Guopan Li","doi":"10.1016/j.acthis.2023.152030","DOIUrl":"10.1016/j.acthis.2023.152030","url":null,"abstract":"<div><p><span><span><span><span>Superoxide dismutase (SOD) is an antioxidant enzyme with multiple metal cofactors that can specifically clear </span>reactive oxygen species (ROS), which plays an important role in a variety of ultraviolet-induced lesions. Therefore, SOD has the anti-ultraviolet radiation effect. The objective of this study was to compare the differences in the anti-ultraviolet radiation effect of SOD with distinct metal cofactors: Cu/Zn-SOD and Mn-SOD. SOD was first purified using </span>hydrophobic interaction chromatography and ion-exchange chromatography. Second, the Methylthiazolyldiphenyl-tetrazolium bromide method and cell senescence kits were used to study the protective effect of SOD on ultraviolet-induced cell damage. Finally, the protective effect of SOD on ultraviolet -induced </span>skin damage was histopathologically evaluated, and the expression levels of </span>malondialdehyde<span><span> (MDA) and matrix metalloproteinases (MMPs) in tissues were detected. The results showed that Cu/Zn-SOD was superior to Mn-SOD in promoting </span>cell proliferation, alleviating cell damage, protecting skin structure, and regulating the expression levels of MDA and MMPs, and it has no side effects. In conclusion, Cu/Zn-SOD had a better anti-ultraviolet radiation effect than Mn-SOD, and it can be used in anti-aging and anti-ultraviolet skin-care products.</span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9914922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advantages of mesenchymal stem cell over the other stem cells","authors":"Janani Gopalarethinam ,&nbsp;Aswathy P. Nair ,&nbsp;Mahalaxmi Iyer ,&nbsp;Balachandar Vellingiri ,&nbsp;Mohana Devi Subramaniam","doi":"10.1016/j.acthis.2023.152041","DOIUrl":"10.1016/j.acthis.2023.152041","url":null,"abstract":"<div><p><span>A stem cell is a particular group of cells that has the extraordinary potential to convert within the body into particular cell types. They are used to regenerate tissues and cells in the body that have been damaged or destroyed by the disease. Stem cells come in three different varieties: adult stem cells<span>, embryonic stem cells and </span></span>induced pluripotent stem cells<span><span> (iPSCs). Embryonic stem cells have a high chance of immune rejection and also have ethical dilemmas and iPSCs have genetic instability. Adult stem cells are difficult to analyze and extract for research since they are frequently insufficient in native tissues. However, </span>mesenchymal stem cells<span><span> (MSC) one of the categories of adult stem cells are stromal cells with a variety of potentials that can differentiate into a wide range of cell types. MSCs can be transplanted into a variety of people without worrying about rejection because they have demonstrated the ability to prevent an adverse reaction from the immune system. These transplants have powerful anti-inflammatory and </span>immunosuppressive<span> effects and greatly enhance the body's inherent healing capacity. While MSCs do not offer treatment<span> for illnesses, the idea behind them is to enable the body to recover sufficiently for a protracted reduction in symptoms. In many cases, this is sufficient to significantly enhance the patient’s well-being. Inspite of several advantages some potential long-term concerns connected to MSC therapy<span> are maldifferentiation, immunosuppression and cancerous tumor growth. In this review, we will compare the mesenchymal stem cells with other stem cells with respect to the source of origin, their properties and therapeutic applications, and discuss the MSC’s disadvantages.</span></span></span></span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9875089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia activates the PI3K/AKT/HIF-1α pathway to promote the anti-inflammatory effect of adipose mesenchymal stem cells","authors":"Hongjing Ren ,&nbsp;Mengchang Liu ,&nbsp;Yueda Jihu ,&nbsp;Huizhen Zeng ,&nbsp;Chong Yao ,&nbsp;Hong Yan","doi":"10.1016/j.acthis.2023.152042","DOIUrl":"10.1016/j.acthis.2023.152042","url":null,"abstract":"<div><p><span>This study aimed to investigate the effect of hypoxia on the anti-inflammatory effect of adipose-derived mesenchymal stem cells (AMSCs) </span><em>in vitro</em> and its possible mechanism. AMSCs were cultured <em>in vitro</em> in a hypoxic environment with 3% O₂, and a normoxic (21% O₂) environment was used as the control. The cells were identified by <em>in vitro</em><span> adipogenic and osteogenic differentiation and cell surface antigen<span> detection, and the cell viability were detected. The effect of hypoxic AMSCs on macrophage inflammation was analyzed by co-culture. The results showed that under hypoxia, AMSCs had better viability, significantly downregulated the expression of inflammatory factors, alleviated macrophage inflammation, and activated the PI3K/AKT/HIF-1α pathway.</span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10136041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The differentiation and generation of glucose-sensitive beta like-cells from menstrual blood-derived stem cells using an optimized differentiation medium with platelet-rich plasma (PRP)","authors":"Atefeh Hojjat ,&nbsp;Reyhaneh Nassiri Mansour ,&nbsp;Seyed Ehsan Enderami ,&nbsp;Hadi Hassannia ,&nbsp;Mohammadreza Mahdavi ,&nbsp;Amir Mellati ,&nbsp;Kayvan Mehdipour chari ,&nbsp;Reza Salarinia ,&nbsp;Ehsan Saburi","doi":"10.1016/j.acthis.2023.152025","DOIUrl":"10.1016/j.acthis.2023.152025","url":null,"abstract":"<div><p><span>Regarding their reversible damage of insulin-producing cells (IPCs) and the inefficiency of treatment methods for </span>type 1 diabetes mellitus<span><span><span> (T1DM), scientists decided to produce IPCs from an unlimited source of cells. But the production of these cells is constantly faced with problems such as low differentiation efficiency in cell therapy and regenerative medicine. This study provided an ideal differentiation medium enriched with plasma-rich platelet (PRP) delivery to produce IPCs from menstrual blood-derived stem cells (MenSCs). We compared them with and without PRP differentiation medium. MenSCs were then cultured in two experimental groups: with/without PRP differentiation medium and a control group (undifferentiated MenSCs). After 18 days, differentiated cells were analyzed for expression of pancreatic gene markers by real-time PCR. </span>Immunocytochemical staining was used to detect the presence of insulin and Pdx-1 in the differentiated cells, and insulin and C-peptide secretion response to glucose were tested by ELISA. Finally, the morphology of differentiated cells was examined by an inverted microscope. </span>In vitro studies<span> showed that MenSCs differentiated in the PRP differentiation medium had strong properties of IPCs such as pancreatic islet-like structure. The expression of pancreatic markers at both RNA and protein levels showed that the differentiation efficiency was higher in the PRP differentiation medium. In both experimental groups, the differentiated cells were functional and secreted C-peptide and insulin on glucose stimulation, but the secretion of C-peptide and insulin in the PRP group was higher than those cultured in the without PRP differentiation medium. Our findings showed that using of PRP enriched differentiation medium can promote the differentiation of MenSCs into IPCs compared to the without PRP culture group. Therefore, the use of PRP into differentiation media can be proposed as a new approach to producing IPCs from MenSCs and used in cell-based therapies for T1DM.</span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9413231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}