Localization of EFA6A, an exchange factor for Arf6, in Z-lines and sarcoplasmic reticulum membranes in addition to myofilaments in I-domains of skeletal myofibers of peri-natal mice

IF 2.3 4区生物学 Q4 CELL BIOLOGY

Acta histochemica Pub Date : 2024-08-09 DOI:10.1016/j.acthis.2024.152187

Surang Chomphoo , Hiroyuki Sakagami , Hisatake Kondo , Wiphawi Hipkaeo

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引用次数: 0

Abstract

Membrane trafficking and actin-remodeling are critical for well-maintained integrity of the cell organization and activity, and they require Arf6 (ADP ribosylation factor 6) activated by GEF (guanine nucleotide exchange factor) including EFA6 (exchange factor for Arf6). In the present immuno-electron microscopic study following previous immunohistochemical study by these authors (Chomphoo et al., 2020) of in situ skeletal myoblasts and myotubes of pre-and perinatal mice, the immunoreactivity for EFA6A was found to be localized at Z-bands and sarcoplasmic reticulum (SR) membranes in I-domains as well as I-domain myofilaments of skeletal myofibers of perinatal mice. Based on the previous finding that EFA6 anchored on the neuronal postsynaptic density via α-actinin which is known to be shared by muscular Z-bands, the present finding suggests that EFA6A is also anchored on Z-bands via α-actinin and involved in the membrane trafficking and actin-remodeling in skeletal myofibers. The localization of EFA6A-immunoreactivity in I-domain SR suggests a differential function in the membrane traffic between the I- and A-domain intracellular membranes in perinatal skeletal myofibers.

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EFA6A（Arf6 的交换因子）在围产期小鼠骨骼肌纤维 I 域的 Z 线和肌浆网膜以及肌丝中的定位。

细胞膜贩运和肌动蛋白重塑对维持细胞组织和活动的完整性至关重要，它们需要 Arf6（ADP 核糖基化因子 6）通过包括 EFA6（Arf6 的交换因子）在内的 GEF（鸟嘌呤核苷酸交换因子）激活。在本免疫电镜研究中，继这些作者之前（Chomphoo 等人，2020 年）对出生前和围产期小鼠的原位骨骼肌母细胞和肌管进行免疫组化研究之后，发现 EFA6A 的免疫反应定位于围产期小鼠骨骼肌纤维 I 域和 I 域肌丝的 Z 带和肌浆网（SR）膜。之前的研究发现，EFA6通过α-肌动蛋白固定在神经元突触后密度上，而已知肌肉Z带也共享α-肌动蛋白，因此本研究结果表明，EFA6A也通过α-肌动蛋白固定在Z带上，并参与骨骼肌纤维的膜运输和肌动蛋白重塑。EFA6A免疫反应在I域SR中的定位表明，它在围产期骨骼肌纤维I域和A域细胞内膜之间的膜运输中发挥着不同的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta histochemica 生物-细胞生物学

CiteScore

4.60

自引率

4.00%

发文量

107

审稿时长

23 days

期刊介绍： Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted