Journal of Natural Medicines最新文献

{"title":"Processing reduces diester diterpenoid alkaloids content of fuzi products, resulting in reduced toxicity and modified bioactivities","authors":"Tian Xiang,&nbsp;Xiaozhou Yang,&nbsp;Xiaoyao Zhang,&nbsp;Haobo Yuan,&nbsp;Man Xu,&nbsp;Chenxuan Yang,&nbsp;Murtala Bindawa Isah,&nbsp;Chen Chen,&nbsp;Hao Han,&nbsp;Xiaoying Zhang","doi":"10.1007/s11418-025-01895-6","DOIUrl":"10.1007/s11418-025-01895-6","url":null,"abstract":"<div><p>Fuzi is a generic term for various processed products of the lateral roots of <i>Aconitum carmichaelii</i> Debeaux, with a long history of medicinal use including hypoglycemic, anti-inflammatory, and immunity-enhancing. However, the toxicity of Fuzi limits its widespread use. Different processing methods have been used to minimize toxicity and improve the medicinal properties of Fuzi. Three processed Fuzi products were prepared according to Chinese Pharmacopoeia and their chemical compositions were qualitatively and quantitatively analysed using UPLC-MS. The toxicity, antioxidant properties and bioactivity changes were assessed in <i>Caenorhabditis elegans</i>. A total of 99 compounds were preliminarily identified, and a subsequent multivariate analysis showed significant differences among the different processed products in terms of chemical compositions. The processing led to a significant loss of alkaloids, decrease in the contents of total polyphenols and flavonoids, and a decrease in antioxidant capacity while increasing the total polysaccharide and uronic acid contents in Yan Fuzi and Hei Shunpian as well as the content of monoester diterpenoid alkaloids in Hei Shunpian and Bai Fupian. Furthermore, the processed products prevented cold stress in <i>C. elegans</i>. In conclusion, processing altered the composition and reduced the toxicity of Fuzi and led to differences in the pharmacological activities of different processed Fuzi products. These results provide a theoretical basis for the in-depth pharmacological study and application of processed products of Fuzi.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"695 - 705"},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the mechanism of Buyang Huanwu decoction in improving learning and memory impairment in Alzheimer's disease mice based on lipidomics","authors":"Jing Jiang,&nbsp;Kai Duo,&nbsp;Siyu Zhu,&nbsp;Yitong Wang,&nbsp;Hui Xue,&nbsp;Chengyu Piao,&nbsp;Yifan Ren,&nbsp;Xia Lei,&nbsp;Yafeng Zhang,&nbsp;Jianxin Liu,&nbsp;Lihong Yang,&nbsp;Ning Zhang","doi":"10.1007/s11418-025-01890-x","DOIUrl":"10.1007/s11418-025-01890-x","url":null,"abstract":"<div><p>In this study, a lipid disorder Alzheimer’s disease (AD) model was developed with high-fat diet and <span>d</span>-galactose injected intraperitoneally (HFD &amp; <span>d</span>-gal) to evaluate the activities of Buyang Huanwu Decoction (BYHWD) compared with donepezil hydrochloride. The learning and memory abilities of BYHWD were evaluated by Morris water maze test (MWM). The lipid levels in serum, histopathology, and immunohistochemistry of hyperphosphorylated tau protein in hippocampal neurons were conducted to prove the therapy effects of BYHWD. After the identification of constituents absorbed into the brain using LC–MS, UPLC-TQ-MS was employed to analyze endogenous lipid metabolites in the hippocampi of mice. Based on the validated differential markers identified through lipidomics analysis, we further substantiated potential therapeutic pathway of BYHWD through the application of molecular docking technology. The mechanism underlying BYHWD was subsequently confirmed by palmitic acid-injured HT22 cells. The results showed that BYHWD significantly improved the cognitive deficits and regulated the lipid levels of HFD &amp; D-gal mice. BYHWD also protected the neuronal cell condition of hippocampal neurons, increased the density of dendritic spines, and reduced the expression of P-tau. Lipidomics revealed that 41 differential lipid metabolites were retuned after BYHWD administration, and this change may be related to the PPARγ pathway. Calycosin-7-glucoside showed good interaction with PPARγ in vivo composition analysis. Calycosin-7-glucoside increased the mRNA expression levels of lipid metabolism-related enzymes and PPARγ, as well as the expression of PPARγ protein in vitro study. BYHWD activated the PPARγ pathway to induce peroxisome proliferation and regulated lipid metabolism disorders in the AD mice brain.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"568 - 590"},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11418-025-01890-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring new natural products by utilizing untapped secondary metabolic pathways in actinomycetes","authors":"Shotaro Hoshino","doi":"10.1007/s11418-025-01903-9","DOIUrl":"10.1007/s11418-025-01903-9","url":null,"abstract":"<div><p>Actinomycetes have produced a variety of bioactive secondary metabolites; however, discovering new actinobacterial natural products using conventional approaches has become increasingly challenging. Meanwhile, genomic studies of actinomycetes have revealed that numerous secondary metabolite biosynthetic gene clusters (SM-BGCs) remain untapped. Thus, utilizing these secondary metabolic pathways is expected to facilitate the discovery of new actinomycetes-derived natural products. In this review, I primarily describe our research on the utilization of these untapped actinobacterial SM-BGCs and the discovery of new secondary metabolites. First, I introduce our studies on the activation of silent SM-BGCs through the co-cultivation of various actinomycetes with mycolic acid-containing bacteria (MACB), which led to the identification of 20 actinobacterial secondary metabolites, including 16 new compounds. In the latter part, I describe our recent findings on arsenic-related secondary metabolism, which has been overlooked in model actinomycetes, including the identification of a novel organoarsenic natural product, and the elucidation of its unique biosynthetic strategy, which is independent of <i>S</i>-adenosylmethionine (SAM)-dependent enzymes.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"465 - 476"},"PeriodicalIF":2.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chaihu Guizhi Ganjiang Decoction ameliorates chronic pancreatitis by modulating the SK1/S1P signaling pathway","authors":"Guo-Wang Yao,&nbsp;Cai-Xia Li,&nbsp;Yu-Xing Fan,&nbsp;Yu-Zhen Zhuo,&nbsp;Shu-Kun Zhang,&nbsp;Li-Hua Cui","doi":"10.1007/s11418-025-01901-x","DOIUrl":"10.1007/s11418-025-01901-x","url":null,"abstract":"<div><p>Chronic pancreatitis (CP) is a progressive disease characterized by injury on pancreatic acinar cells (PACs), ongoing fibrosis, and gradual loss of exocrine and endocrine functions. Sphingosine kinase 1 (SK1) expression is elevated in injured PACs, and its metabolite sphingosine-1-phosphate (S1P) promotes the activation of pancreatic stellate cell (PSC) through autophagy and pyroptosis. Chaihu Guizhi Ganjiang Decoction (CGGD), a traditional Chinese medicine is widely used in the clinical treatment of digestive diseases. However, whether CCGD affects the SK1/S1P axis and relieves pancreatic damage through this pathway remains unknown. In this study, CP rats were treated with CGGD, individually or in combination with S1P and SKI-178 for four weeks to assess the effect of CGGD on pancreatic injury, fibrosis, autophagy and pyroptosis. The results showed that SK1, S1P and S1PR2 levels were increased in the pancreatic tissues of CP rats, while CGGD reduced these levels. Treatment with S1P exacerbated histological damage, promoted fibrosis, accelerated autophagy, and induced pyroptosis. Conversely, SKI-178 suppressed these effects. Notably, CGGD mitigated histological damage, decreased serum amylase and lipase levels, and alleviated pancreatic fibrosis induced by S1P. Furthermore, CGGD downregulated autophagy and pyroptosis induced by S1P, exhibiting an effect comparable to SKI-178 in CP. In conclusion, CGGD ameliorates pancreatic damage by reducing fibrosis, inhibiting autophagy, and suppressing pyroptosis through the SK1/S1P axis.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"706 - 720"},"PeriodicalIF":2.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Ceramicines U–Z from Chisocheton ceramicus and structure–antimalarial activity relationship study","authors":"Alfarius Eko Nugroho,&nbsp;Tomoyuki Komuro,&nbsp;Takuya Kawaguchi,&nbsp;Yusuke Shindo,&nbsp;Chin Piow Wong,&nbsp;Yusuke Hirasawa,&nbsp;Toshio Kaneda,&nbsp;Takahiro Tougan,&nbsp;Toshihiro Horii,&nbsp;A. Hamid A. Hadi,&nbsp;Hiroshi Morita","doi":"10.1007/s11418-025-01898-3","DOIUrl":"10.1007/s11418-025-01898-3","url":null,"abstract":"","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"721 - 721"},"PeriodicalIF":2.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11418-025-01898-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine diminishes the malignant progression of non-small cell lung cancer cells by targeting CDCA5 and CCNA2","authors":"Xin Zhao,&nbsp;Minwen Ha,&nbsp;Lulu Zhou,&nbsp;Yanyun Wang,&nbsp;Ping Li","doi":"10.1007/s11418-025-01885-8","DOIUrl":"10.1007/s11418-025-01885-8","url":null,"abstract":"<div><h3>Background</h3><p>Berberine (BBR), an isoquinoline alkaloid from Coptidis Rhizoma, possesses powerful activities against diverse human malignancies, including non-small cell lung cancer (NSCLC). Nevertheless, the underlying anti-tumor mechanisms of BBR in NSCLC remain poorly understood.</p><h3>Methods</h3><p>NSCLC cells were cultured and treated with various doses (0, 15, 30, and 45 μM) of BBR for 48 h. Cell viability, proliferation, apoptosis, migration, and invasion were detected using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU), flow cytometry, transwell, and wound healing assays. Cell division cycle-associated protein 5 (CDCA5) and Cyclin A2 (CCNA2) mRNA level and protein level were measured using real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assays. After STRING databases prediction, the possible interaction between CDCA5 and CCNA2 was identified using Co-Immunoprecipitation (IP) assays. The biological role of BBR treatment on NSCLC tumor growth was assessed using the xenograft tumor model in vivo.</p><h3>Results</h3><p>BBR treatment blocked NSCLC cell proliferation, migration, invasion, and promoted apoptosis. CDCA5 and CCNA2 levels were increased in NSCLC tissues, whereas their expression was decreased in BBR-induced NSCLC cells. CDCA5 or CCNA2 overexpression might attenuate the inhibitory role of BBR on NSCLC cell malignant behaviors. CDCA5 interacted with CCNA2 to regulate its expression in NSCLC cells. BBR administration blocked NSCLC xenograft growth in vivo.</p><h3>Conclusion</h3><p>BBR hindered NSCLC cell malignant progression partly by modulating CDCA5 and CCNA2, providing a promising therapeutic target for NSCLC treatment.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"530 - 542"},"PeriodicalIF":2.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Ameliorative effect of bofutsushosan (Fangfengtongshengsan) extract on the progression of aging-induced obesity","authors":"Takafumi Saeki,&nbsp;Saya Yamamoto,&nbsp;Junji Akaki,&nbsp;Takahiro Tanaka,&nbsp;Misaki Nakasone,&nbsp;Hidemasa Ikeda,&nbsp;Wei Wang,&nbsp;Makoto Inoue,&nbsp;Yoshiaki Manse,&nbsp;Kiyofumi Ninomiya,&nbsp;Toshio Morikawa","doi":"10.1007/s11418-025-01899-2","DOIUrl":"10.1007/s11418-025-01899-2","url":null,"abstract":"","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"722 - 722"},"PeriodicalIF":2.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11418-025-01899-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amide and phenylpropanoid glycosides from the fruits of Piper longum L. and their anti-inflammatory activity","authors":"Guanghui Gou,&nbsp;Liu Liu,&nbsp;Wenli Bao,&nbsp;Jun Li,&nbsp;Haji Akber Aisa","doi":"10.1007/s11418-025-01893-8","DOIUrl":"10.1007/s11418-025-01893-8","url":null,"abstract":"<div><p>Ten glycosidic compounds (<b>1–10</b>), including two novel amide glycosides and one new phenylpropanoid glycoside, were isolated from the fruits of <i>Piper longum</i> L. These novel compounds were identified as (<i>E</i>)-<i>N</i>-feruloylpiperidine 4′-<i>O</i>-<i>β</i>-<i><span>d</span></i>-glucopyranosyl-(1 → 4)-<i>β</i>-<i><span>d</span></i>-glucopyranoside (<b>1</b>), (<i>E</i>)-<i>N</i>-<i>p</i>-coumaroylpiperidine 4′-<i>O</i>-<i>β</i>-<i><span>d</span></i>-glucopyranosyl-(1 → 4)-<i>β</i>-<i><span>d</span></i>-glucopyranoside (<b>2</b>), and (<i>E</i>)-cinnamyl alcohol 9-<i>O</i>-<i>β</i>-<i><span>d</span></i>-glucopyranosyl-(1 → 4)-<i>α</i>-<i><span>l</span></i>-rhamnose-(1 → 6)-<i>β</i>-<i><span>d</span></i>-glucopyranoside (<b>3</b>) by detailed spectroscopic and spectrometric techniques. Acid hydrolysis was employed to determine the glycosidic linkages, facilitating the structural elucidation of these compounds. The anti-inflammatory activities of all isolated compounds were assessed, and the results demonstrated that compounds <b>8</b> and <b>9</b> exhibited moderate inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"686 - 694"},"PeriodicalIF":2.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Development of a determination method for quality control markers utilizing metabolic profiling and its application on processed Zingiber officinale Roscoe rhizome","authors":"Tomohisa Kanai,&nbsp;Tatsuya Shirahata,&nbsp;Shunsuke Nakamori,&nbsp;Yota Koizumi,&nbsp;Eiichi Kodaira,&nbsp;Noriko Sato,&nbsp;Hiroyuki Fuchino,&nbsp;Noriaki Kawano,&nbsp;Nobuo Kawahara,&nbsp;Takayuki Hoshino,&nbsp;Kayo Yoshimatsu,&nbsp;Yoshinori Kobayashi","doi":"10.1007/s11418-025-01900-y","DOIUrl":"10.1007/s11418-025-01900-y","url":null,"abstract":"","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"723 - 723"},"PeriodicalIF":2.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11418-025-01900-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, characterization, and biological activities of a novel polysaccharide extract from Fructus caryophylli","authors":"Qingtao Yu,&nbsp;Wenzhi Li,&nbsp;Jieyi Long,&nbsp;Ming Liang,&nbsp;Lingli Jiang,&nbsp;Xiaoliang Lin,&nbsp;Dongqing He,&nbsp;Zhuoyan Wu,&nbsp;Xiaole Xia","doi":"10.1007/s11418-025-01891-w","DOIUrl":"10.1007/s11418-025-01891-w","url":null,"abstract":"<div><p>This study showed that Polysaccharide-rich <i>Fructus caryophylli</i> extracts (FCE) were prepared for investigation through hot water extraction. Glucose was found to be the significant monosaccharide by chemical analysis, which included Fourier transform-infrared (FT-IR), high-performance liquid chromatography (HPLC), and high-performance gel permeation chromatography (HPGPC). The average molecular weight of FCE was ranged from 15.19 and 208.53 kDa. The bioactivities of FCE, including antioxidant, whitening, tissue regeneration, and anti-wrinkle properties, were evaluated using both in vitro and in vivo tests. In vitro antioxidant experiments demonstrated scavenging of 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl radicals, and strong reducing power. FCE treatment effectively reduced oxidative stress in an in vivo antioxidant experiment involving zebrafish embryos exposed to a nonlethal dose of LPS, demonstrating its potent antioxidant potential. Furthermore, FCE exhibited promise in decreasing tyrosinase activity and total melanin content in zebrafish embryos, while promoting the relative expression levels of the elastin-regulating gene Eln1 RNA and the collagen-related gene col1a1a, thereby facilitating the positive stimulation of wound healing. This research provides valuable insights into the development of FCE as a novel functional raw material for applications in the food and cosmetics industries.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 3","pages":"673 - 685"},"PeriodicalIF":2.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}