Journal of Natural Medicines最新文献

{"title":"Fufangteng Yixin Formula alleviates myocardial ischemia-reperfusion injury by modulating gut microbiota and resultant metabolites in rats","authors":"Fengyi Li,&nbsp;Haitao Zhao,&nbsp;Yilu Lei,&nbsp;Jinwei Luo,&nbsp;Boling Chen,&nbsp;Chenglin Li,&nbsp;Xuan Zhao,&nbsp;Huizhen Jiang","doi":"10.1007/s11418-026-02065-y","DOIUrl":"10.1007/s11418-026-02065-y","url":null,"abstract":"<div><p>This study aimed to explore whether Fufangteng Yixin Formula (FFTYXF) can ameliorate myocardial ischemia-reperfusion injury (MIRI) by regulating the gut microbiota and resultant metabolites. Wistar rats were given FFTYXF by gavage for 7 days, and MIRI rat model was established. Serum level of inflammatory markers was determined by ELISA, and heart function was evaluated by echocardiography. Myocardial histological changes and infarct size were examined by hematoxylin-eosin and triphenyltetrazolium chloride staining, respectively. 16S rRNA gene and metagenomics analyses were employed to explore gut microbiota, while untargeted metabolomics analysis was used to explore serum metabolites. FFTYXF pretreatment could significantly improve cardiac function, reduce infarct size, decrease level of inflammatory factors (TNF-α and IL-6) and inflammatory cells infiltration. At genus level, g__<i>Oscillibacter</i> and g__<i>Rikenellaceae</i>_<i>RC9</i>_<i>gut</i>_<i>group</i> were identified as key microbial bacteria in MIRI rat response to FFTYXF pretreatment. After FFTYXF pretreatment, the functional categories of gut microbiota were participated in fatty acid (FA) biosynthesis/metabolism, glycolysis _ gluconeogenesis and sphingolipid metabolism. Genes response to FFTYXF pretreatment in MIRI rats included K00023 (phbB), K00281 (GLDC, gcvP), K03879 (ND2), K07827 (KRAS) and K15192 (BTAF1), and they were mainly participated in carbon, butanoate, glyoxylate and dicarboxylate metabolism. Differential metabolites were also mainly participated in FA biosynthesis/metabolism, such as alpha-Linolenic acid, omega-3/omega-6 FA, and flavan-3-ol metabolic pathway. Abundance of g__<i>Rikenellaceae</i>_<i>RC9</i>_<i>gut</i>_<i>group</i> positively correlated with differential metabolites FAHFA 34:0, FAHFA 16:1/18:3, and FA 24:5. FFTYXF could alleviate MIRI by modulating gut microbial bacteria alteration and resultant metabolites, particularly short-chain FAs.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1521 - 1537"},"PeriodicalIF":2.6,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148597443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetyl group dictates macrophage immune activation by polysaccharide ME-2 via TLR4-NF-κB/MAPK pathways","authors":"Nan Wang,&nbsp;Lan Zhou,&nbsp;Si-Liang Jiang,&nbsp;Wen-Fei Wang,&nbsp;Hai-Xue Kuang,&nbsp;Jun Liang,&nbsp;Yong-Gang Xia","doi":"10.1007/s11418-026-02022-9","DOIUrl":"10.1007/s11418-026-02022-9","url":null,"abstract":"<div><p>ME-2, a GXG’G’’M type polysaccharide with a high <i>O</i>-acetyl group content, was isolated and purified from <i>Auricularia auricula</i>-judae. However, the mechanism underlying its immunomodulatory activity has not been thoroughly investigated. This study demonstrates that ME-2 effectively reverses cyclophosphamide-induced immunosuppression in vivo by normalizing organ indices, increasing serum cytokine levels, and restoring the structure of the spleen tissue. In vitro, ME-2 binds to TLR4 on the surface of RAW264.7 cells. This binding triggered the activation of both the MAPK and NF-κB signaling pathways. Consequently, ME-2 treatment promoted the secretion of immune effector factors, enhanced endocytic activity, and induced a shift toward the M1 phenotype. Subsequently, the above mechanism was reversely validated through TLR4 knockdown and specific signaling pathway inhibitors. These results demonstrate that ME-2 exhibits a potent immune activation effect, laying a foundation for the development of ME-2-based immunomodulators. In addition, experimental results demonstrated that dME-2 with complete de-<i>O</i>-acetylation exhibits no binding affinity for TLR4 and fails to activate its downstream signaling pathways. These findings confirm that <i>O</i>-acetyl groups are indispensable for ME-2-mediated immune activation.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1339 - 1356"},"PeriodicalIF":2.6,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin attenuates cisplatin-induced muscle atrophy via Hippo/YAP1 signaling pathway","authors":"Jingchun Lv,&nbsp;Jianwei Lin,&nbsp;Xiaojing Chen,&nbsp;Qi Wu,&nbsp;Zhiyao Cao,&nbsp;Dapeng Jiang","doi":"10.1007/s11418-026-02062-1","DOIUrl":"10.1007/s11418-026-02062-1","url":null,"abstract":"<div><p>Muscle wasting is a common event among cancer patients receiving chemotherapy treatment and has been reported to affect their survival. However, current therapies for counteracting this side effect are ineffective. This study investigates quercetin’s efficacy against cisplatin-induced muscle atrophy and its mechanism. Using both C2C12 myotube and mouse models, we found quercetin pretreatment significantly alleviates cisplatin-induced muscle wasting. Mechanistically, transcriptome analysis identified that the Hippo signaling pathway was involved in cisplatin-induced muscle atrophy. Quercetin restored the activity of this pathway, including the expression and nuclear localization of its effector YAP1. Furthermore, quercetin mitigated cisplatin-induced muscle damage by improving mitochondrial membrane quality and function. Molecular docking revealed a direct interaction between quercetin and YAP1. Additionally, the possible interaction between YAP1 and mitochondrial function was revealed. Our findings demonstrate that quercetin attenuates cisplatin-induced muscle atrophy by modulating the Hippo/YAP1 pathway and preserving mitochondrial homeostasis, highlighting its therapeutic potential.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1446 - 1460"},"PeriodicalIF":2.6,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two novel compounds (lovastatin analog Y and lovastatin analog Z) isolated from health foods containing beni-koji (red yeast rice) with adverse events report","authors":"Seiji Tanaka,&nbsp;Naoko Masumoto,&nbsp;Takuya Makino,&nbsp;Yuji Matsushima,&nbsp;Toshio Morikawa,&nbsp;Michiho Ito","doi":"10.1007/s11418-026-02041-6","DOIUrl":"10.1007/s11418-026-02041-6","url":null,"abstract":"<div><p>Ingestion of a contaminated health food product containing beni-koji (red yeast rice), one of the so-called “Foods with Functional Claims (FFC),’’ was recently reported to have adverse health effects, such as kidney damage. We previously revealed the presence of unexpected components, namely lovastatin analog Y and lovastatin analog Z, in contaminated FFC samples, which, incidentally, also contained puberulic acid. However, the structures and stereochemistries of these novel compounds remain unknown. In this study, we addressed this by conducting a comprehensive instrumental analysis of lovastatin analogs Y and Z isolated from the FFC samples. The two-dimensional structures of the isolated compounds were determined by ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and NMR spectroscopy. Their absolute configurations were established by comparison with those of chemically synthesized lovastatin analogs Y and Z. X-ray structural analysis using the crystalline sponge method confirmed that the synthesized lovastatin analogs Y and Z have the same absolute configuration as lovastatin acid. UHPLC-HRMS and NMR analyses further showed that the absolute configurations of lovastatin analogs Y and Z isolated from the FFC samples are identical to those of the synthesized compounds. Considering that lovastatin analogs Y and Z are produced through the co-incubation of <i>Monascus pilosus</i> and <i>Penicillium adametzioides</i>, these findings are expected to contribute to biosynthetic research related to these fungal species.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1367 - 1383"},"PeriodicalIF":2.6,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ephedplics E-I: three lignans and two benzofuran-type compounds from the roots of Ephedra sinica Stapf and their neuroprotective activity","authors":"Yunlong Dou,&nbsp;Bowen Zhang,&nbsp;Meng Li,&nbsp;Xiaolan Wang,&nbsp;Shuyan Liu,&nbsp;Yanjun Sun,&nbsp;Xiaoke Zheng,&nbsp;Weisheng Feng","doi":"10.1007/s11418-026-02036-3","DOIUrl":"10.1007/s11418-026-02036-3","url":null,"abstract":"<div><p>Five new compounds Ephedplics E-I were isolated from the roots of <i>Ephedra sinica</i> Stapf, including three lignans (<b>1–3</b>) and two benzofuran-type compounds (<b>4–5</b>). Their structures were elucidated by spectroscopic methods, involving UV, IR, NMR spectra and HRESIMS analyses. The absolute configuration of compounds <b>1–5</b> was determined by comparing their experimental and calculated ECD spectra. All compounds were assessed for their neuroprotective activities against damage induced by corticosterone (CORT) in PC-12 cells. Compounds <b>1</b> and <b>2</b> were capable of significantly ameliorating CORT-induced PC-12 injury.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1357 - 1366"},"PeriodicalIF":2.6,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Garcinuntones A‒C, three new polyprenylated xanthones with cytotoxic and anti-HIV-1 activities from Garcinia nuntasaenii","authors":"Suppisak Chaturonrutsamee,&nbsp;Chutima Kuhakarn,&nbsp;Sariyarach Thanasansurapoung,&nbsp;Samran Prabpai,&nbsp;Arthit Chairoungdua,&nbsp;Radeekorn Akkarawongsapat,&nbsp;Narong Nuntasaen,&nbsp;Sakchai Hongthong,&nbsp;Bodee Nutho,&nbsp;Vichai Reutrakul","doi":"10.1007/s11418-026-02060-3","DOIUrl":"10.1007/s11418-026-02060-3","url":null,"abstract":"<div><p>Three previously undescribed polyprenylated xanthone derivatives named garcinuntones A‒C (<b>1</b> − <b>3</b>), together with fifteen known compounds (<b>4</b> − <b>18</b>) were isolated from the leaves and twigs of <i>Garcinia nuntasaenii</i> Ngerns. &amp; Suddee (Clusiaceae). Their structures were determined by analysis of their physical and spectroscopic data and by comparison with those of related compounds reported in the literature. The absolute configuration of garcinuntone B (<b>2</b>) was established by single crystal X-ray crystallographic analysis using Cu Kα radiation data. Cytotoxic activity against several cancer cell lines (P-388, KB, HT-29, MCF-7, A549, and ASK) and anti-HIV-1 activity using syncytium inhibition and anti-HIV-1 reverse transcriptase assays of some isolated compounds were evaluated. Garcinuntone B exhibited cytotoxicity against some tested cell lines with IC₅₀ values in the range of 4.82 to 6.92 μM. In anti-HIV-1 assays, garcinuntone B demonstrated selective activity against the reverse transcriptase (RT) enzyme, showing an IC₅₀ of 23.61 µM, while it proved to be toxic to the host cell line in the syncytium inhibition assay. In addition, the binding affinity of garcinuntone B to HIV-1 RT was assessed through molecular docking study. Like other non-nucleoside RT inhibitors, garcinuntone B showed stable number of contacts with the binding residues in the binding pocket of HIV-1 RT enzyme.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1433 - 1445"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11418-026-02060-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148366533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative pharmaco dynamic material basis and COX-2/PGE2-mediated anti-RA mechanism of wild and simulated-wild cultivated Saposhnikovia divaricata","authors":"Xinwei Nie,&nbsp;Mingyue Zhou,&nbsp;Dingfei Yang,&nbsp;Jingyan Hu,&nbsp;Yuxuan Zou,&nbsp;Zihan Yang,&nbsp;Yulan Wang,&nbsp;Wenqian Xie,&nbsp;Shengli Wei","doi":"10.1007/s11418-026-02049-y","DOIUrl":"10.1007/s11418-026-02049-y","url":null,"abstract":"<div><p>Saposhnikoviae Radix (SR) is a traditional Chinese herbal medicine widely used for the treatment of “Bi syndrome”. The cultivation patterns of SR have been adjusted in response to wild resource scarcity. Different cultivation patterns directly affect the appearance, chemical constituents and pharmacological efficacy of SR. However, the mechanism of SR from various patterns against rheumatoid arthritis (RA) remains unclear. This study systematically studied morphological characteristics, chemical constituents, and therapeutic efficacy against RA between wild-sourced SR (WS-SR) and simulated-wild cultivated SR (SWC-SR). Putative bioactive constituents and their underlying target pathways were prioritized through integrated LC-MS analysis and network pharmacology. The therapeutic efficacy was validated using an adjuvant-induced arthritis (AIA) rat model, while the molecular mechanisms of putative active constituents were elucidated in MH7A cells, focusing on the modulation of the COX-2/PGE2 signaling pathway. Our findings demonstrated that both WS-SR and SWC-SR exerted significant anti-RA efficacy. Notably, 5-<i>O</i>-methylvisammioside was identified as a potential bioactive constituent which significantly inhibited inflammatory responses <i>via</i> the COX-2/PGE2 signaling pathway. By elucidating the critical pathways and main targets of SR, this study provided new insights evidence to support the feasibility of simulated-wild cultivation as a viable and effective alternative to wild populations for sustainable medicinal production.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1384 - 1402"},"PeriodicalIF":2.6,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tinosporide promotes melanogenesis via activation of cAMP/PKA/CREB–MITF signaling in B16F10 and SK-MEL-2 cells","authors":"Sukhamoy Dhabal,&nbsp;Anirban Kr Chakrabarty,&nbsp;Rajiva Kumar Rai,&nbsp;Satyabrata Mohapatra,&nbsp;Rahul Singh,&nbsp;Chandra Kant Katiyar,&nbsp;Sunil Kumar Dubey","doi":"10.1007/s11418-026-02054-1","DOIUrl":"10.1007/s11418-026-02054-1","url":null,"abstract":"<div><p>Hypopigmentation disorders are characterized by impaired melanogenesis and remain challenging to manage due to limited efficacy of current interventions and insufficient mechanistic insights. Natural products represent a valuable source of bioactive phytoconstituents capable of regulating key pathways involved in melanin synthesis. Despite its extensive traditional use and diverse pharmacological activities, <i>Tinospora cordifolia</i> and its major bioactive constituent tinosporide remain unexplored for their role in melanogenesis. The present study aimed to evaluate the melanogenesis-promoting potential of bioactive phytoconstituents isolated from <i>Tinospora cordifolia</i>, and to elucidate their underlying molecular mechanisms. Four phytoconstituents–columbin, tinosporide, 8-hydroxy tinosporide and ecdysterone–were isolated and structurally characterized from <i>T. cordifolia.</i> Their melanogenic potential was evaluated in murine (B16F10) and human (SK-MEL-2) melanoma cells by assessing melanin content, tyrosinase activity, and expression of melanogenesis-associated genes. Intracellular cAMP levels and associated signalling pathways were further analysed to investigate the mechanism of action. Among the isolated compounds, tinosporide exhibited the most pronounced melanogenic activity, significantly increasing melanin synthesis and tyrosinase activity. Tinosporide promotes nuclear localization of the microphthalmia-associated transcription factor (MITF), leading to upregulation of MITF-target genes, including <i>Trp1</i>, <i>Tyr</i>, <i>Dct</i>, <i>Pmel</i>, and <i>Mlana.</i> Furthermore, mechanistic studies revealed that tinosporide elevated intracellular cAMP levels and induced the phosphorylation of PKA and CREB, thereby activating cAMP/PKA/CREB signalling pathway. Pharmacological inhibition of PKA and CREB markedly attenuated tinosporide-induced melanogenesis, confirming the essential role of the cAMP/PKA/CREB–MITF axis. Collectively, these findings identify tinosporide as a promising natural melanogenic agent derived from <i>Tinospora cordifolia</i> and highlight its potential relevance in the development of plant-based interventions for hypopigmentation disorders.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>According to the Submission Guidelines, the graphics for chemical structure tinosporideshould be created using drawing programs such as ChemDraw (ChemBioDraw) with the ACS 1996 document settings.</p></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1403 - 1416"},"PeriodicalIF":2.6,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supporting exploration and sustainable utilization of the medicinal plant Uncaria rhynchophylla in Kyushu, Japan through potential distribution modeling using MaxEnt and GIS","authors":"Toshiyuki Atsumi,&nbsp;Suzuka Hokazono,&nbsp;Kaito Kikuchi,&nbsp;Taichi Fujii,&nbsp;Kiyoshi Takejima,&nbsp;Mikiyo Wada,&nbsp;Katsunori Miyake,&nbsp;Tadahiro Yahagi,&nbsp;Kenichi Nakamura,&nbsp;Akihito Takano,&nbsp;Motoyasu Minami","doi":"10.1007/s11418-026-02058-x","DOIUrl":"10.1007/s11418-026-02058-x","url":null,"abstract":"<div><p>Sustainable utilization of wild medicinal plant resources requires reproducible approaches for locating and managing natural populations. In Japan, exploration and resource planning for wild medicinal plants rely heavily on expert knowledge, and reproducible approaches remain scarce. We developed a species distribution model (SDM) for <i>Uncaria rhynchophylla</i> (Rubiaceae), a botanical source of the crude drug Uncaria Hook used in Kampo medicine, to estimate its potential distribution and identify major environmental correlates in the Kyushu region, southern Japan. Using 122 occurrence records collected from 2021 to 2023 and nine environmental predictors, MaxEnt models were trained with background points and bootstrap replicates. Because mean minimum winter temperature in January and mean maximum summer temperature in August were highly correlated, three candidate models were compared using the small-sample corrected Akaike information criterion (AICc), test omission rates, and test area under the receiver operating characteristic curve (AUC). The model including winter minimum temperature was best supported (test AUC = 0.82; test omission rate = 24%), whereas adding summer maximum temperature provided limited improvement (ΔAICc = + 75.75). Variable importance and jackknife tests ranked winter minimum temperature as the most influential predictor, followed by slope angle and distance to the nearest rivers. The suitable area, defined using the maximum training sensitivity plus specificity (MTSS) threshold, covered 10,595 km² (25.9% of the terrestrial area) and formed continuous zones across hilly and low-montane regions. Targeted surveys in high-suitability areas confirmed three additional sites in the Kirishima Mountains. Overall, integrating SDM and geographic information systems provides a reproducible, data-driven, decision-support framework for the exploration, planning of sustainable harvesting, and resource management of wild medicinal plants.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 5","pages":"1417 - 1432"},"PeriodicalIF":2.6,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid chromatography–mass spectrometry-based automated annotation program-assisted structural characterization of daphnane diterpenoids from Daphne genkwa","authors":"Kouharu Otsuki,&nbsp;Mi Zhang,&nbsp;Kakeru Hirose,&nbsp;Takashi Kikuchi,&nbsp;Wei Li","doi":"10.1007/s11418-026-02056-z","DOIUrl":"10.1007/s11418-026-02056-z","url":null,"abstract":"<div><p><i>Daphne genkwa</i> is a deciduous shrub distributed throughout East Asia and has long been used in traditional medicine. Previous phytochemical investigations have shown that <i>D. genkwa</i> is a rich source of bioactive daphnane diterpenoids. In the present study, a comprehensive structural characterization of daphnane diterpenoids in the flowers of <i>D. genkwa</i> was carried out using an LC–MS/MS-based approach. This approach integrated automated annotation program, manual inspection of MS/MS fragmentation pathways, and phytochemical investigation. It led to the structural characterization of 37 daphnane diterpenoids, including nine previously undescribed compounds. These findings demonstrated that <i>D. genkwa</i> flowers contain structurally diverse daphnane diterpenoids, mainly daphnane orthoesters and polyhydroxy daphnanes. Moreover, the diagnostic MS/MS fragmentation and retention time features established in this study provide reliable LC–MS/MS-based criteria for discriminating closely related structural isomers of polyhydroxy daphnanes that differ in their B-ring structures and acylation patterns.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"80 4","pages":"1188 - 1198"},"PeriodicalIF":2.6,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}