Journal of Natural Medicines最新文献

{"title":"Dendrobium nobile Lindl. alkaloids protect CCl₄-induced acute liver injury via upregulating LAMP1 expression and activating autophagy flux.","authors":"Nan Nan, Yonggang Yang, Xiaolong Fu, Siting Xian, Qin Wu, Jingshan Shi, Shaoyu Zhou","doi":"10.1007/s11418-024-01852-9","DOIUrl":"https://doi.org/10.1007/s11418-024-01852-9","url":null,"abstract":"<p><p>Dendrobium nobile Lindl. alkaloids (DNLA) are considered important active ingredients of Dendrobium, which have a variety of pharmacological functions. Recent studies indicate that DNLA has beneficial activity in acute liver injury. However, the specific mechanism by which DNLA produces liver protective effects is stills unclear. This study was designed to determine whether regulation of autophagy is involved in the mode of action of DNLA in liver protection. Using CCl₄-induced acute liver injury (ALI) and cell culture models, the molecular mechanism of DNLA-mediated autophagy regulation was studied. The results showed that DNLA significantly improved CCl₄-induced liver damage and oxidative stress, which was confirmed in AML-12 cells. DNLA promoted autophagy in cells treated with CCl₄, manifested by reduced protein expressions of p62 and LC3-II. Fluorescence imaging showed a decrease in the number of autophagosomes in AML-12 cells transfected with mCherry-GFP-LC3B. In addition, DNLA inhibited lysosomal membrane permeabilization by upregulating lysosomal associated membrane protein-1 (LAMP1), thereby promoting autophagy, preventing CCl₄-induced mitochondrial dysfunction, and reducing the production of mitochondrial reactive oxygen species (ROS). While pretreatment of cells with lysosomal inhibitor chloroquine weakened mitochondrial protection elicited by DNLA, overexpression of mitochondrial-targeted SOD2 in AML-12 cells significantly blocked CCl₄ induced downregulation of LAMP1, thereby improving lysosome integrity and promoting lysosome dependent autophagy, suggesting that there may exist a bidirectional regulation between mitochondrial ROS and lysosome-autophagy activation. Collectively, these results demonstrated that DNLA can protect the liver injury mediated by dysregulation of lysosome-autophagy process through promoting ROS-lysosome-autophagy axis and improving mitochondrial damage.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cathagines A-D, new bisindole alkaloids from Catharanthus roseus.","authors":"Yusuke Hirasawa, Chiaki Kasagi, Erika Koyama, Hitomi Myojin, Takahiro Tougan, Toshihiro Horii, Nahoko Uchiyama, Toshio Kaneda, Hiroshi Morita","doi":"10.1007/s11418-024-01857-4","DOIUrl":"https://doi.org/10.1007/s11418-024-01857-4","url":null,"abstract":"<p><p>Four new bisindole alkaloids, cathagines A (1)-D (4) consisting of an aspidosperma and the fused tetracyclic 3-spirooxindole derived from an iboga type skeleton were isolated from the whole plant of Catharanthus roseus. The structures including absolute stereochemistry were elucidated on the basis of 2D NMR data and CD spectra. Cathagine B (2) showed moderate anti-malarial activity against Plasmodium falciparum 3D7.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daphnepedunins G and H, anti-HIV macrocyclic 3,4-seco-daphnane orthoesters from Daphne pedunculata","authors":"Lingjian Tan,&nbsp;Kouharu Otsuki,&nbsp;Mi Zhang,&nbsp;Takashi Kikuchi,&nbsp;Di Zhou,&nbsp;Ning Li,&nbsp;Li Huang,&nbsp;Chin-Ho Chen,&nbsp;Wei Li","doi":"10.1007/s11418-023-01750-6","DOIUrl":"10.1007/s11418-023-01750-6","url":null,"abstract":"<div><p>Daphnepedunins G (<b>1</b>) and H (<b>2</b>) with unusual macrocyclic 3,4-<i>seco</i>-daphnane orthoester structure were isolated from <i>Daphne pedunculata</i>. Their structures were determined by physicochemical and spectroscopic analyses combined with synthetic methods, including methyl esterification, derivatization reaction using a chiral anisotropic agent, and biomimetic conversion. Compounds <b>1</b> and <b>2</b> along with their methyl esters <b>1a</b> and <b>2a</b> were evaluated for anti-HIV activity, among which <b>1a</b> and <b>2a</b> exhibited potent activity with IC₅₀ values of 1.08 and 1.17 μM, respectively.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"114 - 122"},"PeriodicalIF":2.5,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10235927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of Panax ginseng cultivated in Japan and its relation with some plant characteristics","authors":"Honoka Ito,&nbsp;Michiho Ito","doi":"10.1007/s11418-023-01747-1","DOIUrl":"10.1007/s11418-023-01747-1","url":null,"abstract":"<div><p>In East Asia, <i>Panax ginseng</i> is one of the most important medicinal plants and has been used in traditional medicines from ancient times. Today, <i>P. ginseng</i> is cultivated in Korea, China, and Japan. Although the genetic diversity of <i>P. ginseng</i> in Korea and China has been reported previously, that of <i>P. ginseng</i> cultivated in Japan is largely unknown. In the present study, genetic diversity of <i>P. ginseng</i> cultivated in Japan was analyzed using eight simple sequence repeat markers that have been used in other studies, and the results were compared with previous results for Korea and China. The correlation between genetic diversity and plant characteristics, such as ginsenoside contents, were also examined. The genetic diversity of <i>P. ginseng</i> in Japan was substantially different from that in Korea and China, probably due to Japan’s history of cultivation and the ginseng reproduction system of agamospermy. The genetic analysis indicated that <i>P. ginseng</i> cultivated in Japan could be classified into two clusters. The classification was related to the contents of ginsenosides Re and Ro in the main root but not to the cultivation region of the samples. These results may be useful for the cultivation and quality control of <i>P. ginseng</i> in Japan.</p></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"91 - 99"},"PeriodicalIF":2.5,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10233023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ligustilide enhances pregnancy outcomes via improvement of endometrial receptivity and promotion of endometrial angiogenesis in rats","authors":"Dongjie He,&nbsp;Yanli Song,&nbsp;Huidongzi Xiao,&nbsp;Shaoqi Shi,&nbsp;Hongyan Song,&nbsp;Tianwei Cui,&nbsp;Tingting Ni,&nbsp;Jing Wang,&nbsp;Xingxing Ren,&nbsp;Aiwu Wei","doi":"10.1007/s11418-023-01739-1","DOIUrl":"10.1007/s11418-023-01739-1","url":null,"abstract":"<div><p>Ligustilide (LIG) is the main active ingredient of <i>Angelica sinensis</i> (Oliv.) Diels, which could promote focal angiogenesis to exert neuroprotection. However, there was no report that verified the exact effects of LIG on endometrial angiogenesis and the pregnancy outcomes. To explore the effects of LIG on low endometrial receptivity (LER) and angiogenesis, pregnancy rats were assigned into Control (saline treatment), LER (hydroxyurea-adrenaline treatment), LIG 20 mg/kg and LIG 40 mg/kg groups. Hematoxylin and eosin (H&amp;E) staining was performed to evaluate endometrial morphology. Quantitative real-time PCR, immunofluorescence staining, western blot and immunohistochemistry staining were employed to assess the expression of endometrial receptivity factors and angiogenesis-related gene/protein, respectively. RNA sequencing was used to analyze the effects of LIG on LER caused by Kidney deficiency and blood stasis. We found that endometrial thickness and the implanted embryo number were substantially reduced in the hydroxyurea-adrenaline-treated pregnancy rats. At the same time, the gene and protein expressions of ERα, LIF, VEGFA and CD31 in the endometrium were markedly reduced, while the expressions of MUC1, E-cadherin were increased in the LER group. Administration of LIG raised the endometrial thickness and implanted embryos, as well as reversed the expressions of these factors. Collectively, our findings revealed that LIG could facilitate embryo implantation via recovery of the endometrium receptivity and promotion of endometrial angiogenesis.</p></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"42 - 52"},"PeriodicalIF":2.5,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10214475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ceramicines U–Z from Chisocheton ceramicus and structure–antimalarial activity relationship study","authors":"Alfarius Eko Nugroho,&nbsp;Tomoyuki Komuro,&nbsp;Takuya Kawaguchi,&nbsp;Yusuke Shindo,&nbsp;Chin Piow Wong,&nbsp;Yusuke Hirasawa,&nbsp;Toshio Kaneda,&nbsp;Takahiro Tougan,&nbsp;Toshihiro Horii,&nbsp;A. Hamid A. Hadi,&nbsp;Hiroshi Morita","doi":"10.1007/s11418-023-01746-2","DOIUrl":"10.1007/s11418-023-01746-2","url":null,"abstract":"<div><p>Ceramicines are a series of limonoids which were isolated from the barks of Malaysian <i>Chisocheton ceramicus</i> (Meliaceae), and were known to show various biological activity. Six new limonoids, ceramicines U–Z (<b>1</b>–<b>6</b>), with a cyclopentanone[α]phenanthrene ring system with a β-furyl ring at C-17 were isolated from the barks of <i>C. ceramicus</i>. Their structures were determined on the basis of the 1D and 2D NMR analyses, and their absolute configurations were investigated by CD spectroscopy. Ceramicine W (<b>3</b>) exhibited potent antimalarial activity against <i>Plasmodium falciparum</i> 3D7 strain with IC₅₀ value of 1.2 µM. In addition, the structure–antimalarial activity relationship (SAR) of the ceramicines was investigated to identify substituent patterns that may enhance activity. It appears that ring B and the functional groups in the vicinity of rings B and C are critical for the antimalarial activity of the ceramicines. In particular, bulky ester substituents with equatorial orientation at C-7 and C-12 greatly increase the antimalarial activity.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"68 - 77"},"PeriodicalIF":2.5,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10256577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isovincathicine from Catharanthus roseus induces apoptosis in A549 cells","authors":"Mizuki Hiroki,&nbsp;Aishanjiang Abulikemu,&nbsp;Chihiro Totsuka,&nbsp;Yusuke Hirasawa,&nbsp;Toshio Kaneda,&nbsp;Hiroshi Morita","doi":"10.1007/s11418-023-01740-8","DOIUrl":"10.1007/s11418-023-01740-8","url":null,"abstract":"<div><p>A dimeric indole alkaloid, isovincathicine consisting of an aspidosperma type and modified iboga with C-7–C-20 connection type skeletons was first isolated from <i>Catharanthus roseus</i>, and the structure including stereochemistry was elucidated on the basis of spectroscopic data as well as DP4 statistical analysis. Isovincathicine inhibited cell proliferation in A549 cells. We investigated the detailed mode of action of isovincathicine-induced inhibitory effects on cell proliferation in A549 cells. Flow cytometric analysis showed that isovincathicine-treated cells accumulated in the G₂ phase after 24 h, and the percentage of cells showing cell death increased after 48 h. Western blotting also showed increased expression of BimEL, an apoptosis-related protein, and decreased expression of Mcl-1 and Bcl-xL. Isovincathicine was suggested to induce apoptosis in A549 cells by a mechanism is similar to that of vinblastine.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"216 - 225"},"PeriodicalIF":2.5,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10508805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway","authors":"Xiao-Jun Li,&nbsp;Ting Liu,&nbsp;Yuan Wang","doi":"10.1007/s11418-023-01745-3","DOIUrl":"10.1007/s11418-023-01745-3","url":null,"abstract":"<div><p>Acute kidney injury (AKI) is a complication that can be induced by different factors. Allicin is a class of organic sulfur compounds with anticancer and antibacterial effects, and has not been reported in sepsis-induced AKI (S-AKI). S-AKI was induced in c57BL/6 mice by cecal ligation puncture. In response to the treatment of allicin, the survival rate of mice with S-AKI was increased. Reduced levels of serum creatinine, blood urea nitrogen, UALB, KIM-1 and NGAL indicated an improvement in renal function of S-AKI mice. Allicin inhibited the inflammation and cell apoptosis, which evidenced by decreased levels of inflammatory cytokines and apoptosis-related proteins. Oxidative stress was evaluated by the levels of oxidative stress biomarkers, and suppressed by allicin. In addition, allicin-alleviated mitochondrial dysfunction was characterized by decreased JC-1 green monomer. These effects of allicin were also evidenced in HK2 cells primed with lipopolysaccharide (LPS). Both in vivo and in vitro experiments showed that the nuclear translocation of Nrf2 and the expression of HO-1 increased after allicin treatment, which was confirmed by ML385 and CDDO-Me. In summary, this study revealed the alleviating effect of allicin on S-AKI and demonstrated the promotive effect of allicin on nuclear translocation of Nrf2 for the first time. It was inferred that allicin inhibited the progression of S-AKI through Nrf2/HO-1 signaling pathway. This study makes contributions to the understanding of the roles of allicin in S-AKI.</p></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"53 - 67"},"PeriodicalIF":2.5,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10764392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10508807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic activities of alkaloid constituents from the climbing stems and rhizomes of Sinomenium acutum against cancer stem cells","authors":"Masaya Okayama,&nbsp;Takahiro Matsumoto,&nbsp;Takahiro Kitagawa,&nbsp;Seikou Nakamura,&nbsp;Tomoe Ohta,&nbsp;Tatsusada Yoshida,&nbsp;Tetsushi Watanabe","doi":"10.1007/s11418-023-01744-4","DOIUrl":"10.1007/s11418-023-01744-4","url":null,"abstract":"<div><p>From the methanolic extract of the climbing stems and rhizomes of <i>Sinomenium acutum</i>, two new aporphine analogues, acutumalkaloids I and II, were isolated together with fifteen known compounds including lysicamine. The chemical structures of the isolated new compounds were elucidated based on chemical/physicochemical evidence such as NMR and MS spectra. For acutumalkaloids I and II, the absolute configurations were established by comparison of experimental and predicted electronic circular dichroism (ECD) data. We compared anti-proliferative activities of isolated compounds with reported naturally occurring Wnt/<i>β</i>-catenin pathway inhibitor, nuciferine. Among the isolated compounds, we found lysicamine have anti-proliferative activity against both of HT-29 human colon cancer cell line and its cancer stem cells (CSCs). The IC₅₀ values of lysicamine against non-CSCs and its CSCs were lower than that of nuciferine. In addition, the results of western blotting analysis suggested that lysicamine inhibited the expression of Wnt/<i>β</i>-catenin pathway target protein such as survivin. These results suggested that lysicamine show cytotoxic activity via inhibition of Wnt/<i>β</i>-catenin pathway.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"226 - 235"},"PeriodicalIF":2.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10484787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Euchronin A–F isolated from the Arnebia euchroma (Royle) Johnst. and their anti-proliferative activities in vitro","authors":"Zhen-Peng Zhang,&nbsp;Yan Liu,&nbsp;Hai-Dan Zou,&nbsp;Juan Pan,&nbsp;Zhi-Chao Hao,&nbsp;Wei Guan,&nbsp;Adnan Mohammed Algradi,&nbsp;Hai-Xue Kuang,&nbsp;Bing-You Yang","doi":"10.1007/s11418-023-01738-2","DOIUrl":"10.1007/s11418-023-01738-2","url":null,"abstract":"<div><p>Six new naphthoquinones, euchronin A–F (<b>1</b>–<b>6</b>) and nine known naphthoquinones (<b>7</b>–<b>15</b>), were isolated from the roots of <i>Arnebia euchroma</i> (Royle) Johnst. The structures of the new compounds were confirmed by extensive spectroscopic analyses, including UV, IR, HR-ESI–MS, 1D and 2D NMR. In the present study, we estimated the anti-proliferative activities of these compounds with HaCaT cells. The results indicated that compounds <b>2</b> and <b>4</b> showed strong anti-proliferative activities at 25 μM, with relative viability at 38.83% and 68.44%, respectively.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 1","pages":"33 - 41"},"PeriodicalIF":2.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10135751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}