Journal of Natural Medicines最新文献

{"title":"New cerebrosides and ceramide with other constituents from the aerial parts of Raphidiocystis mannii Hook. f. and their cytotoxic activities.","authors":"Yves M Mba Nguekeu, Herman D Sonfack Fozeng, Chika Ifeanyi Chukwuma, Saw Y Yu Hnin, Nhat Nam Hoang, Emmanuel Mfotie Njoya, Silvère Augustin Ngouela, Mathieu Tene, Tshepiso Jan Makhafola, Hiroyuki Morita, Maurice Ducret Awouafack","doi":"10.1007/s11418-024-01875-2","DOIUrl":"https://doi.org/10.1007/s11418-024-01875-2","url":null,"abstract":"<p><p>Two new cerebrosides, raphimanosides A (1) and B (2), and a new ceramide, raphimanide (3), were isolated from the aerial parts of Raphidiocystis mannii Hook. f., along with ten known compounds (4-13). Their structures were fully established, based on extensive analyses of ¹H, ¹³C, DEPT, 2D NMR, ESIMS, and HRESIMS data and chemical conversion. Subsequently, methanol extract, ethyl acetate, and n-butanol soluble portions and the isolated compounds 1-9, 12, and 13 were assayed for their cytotoxic effects against three human cancer cell lines (MCF-7, HeLa and A549). None of the new compounds demonstrated efficacy against the tested cell lines. In contrast, the methanol extract, ethyl acetate soluble portion, and compounds 6, 7, 9, 12, and 13 showed moderate to low activities against different tested cell lines. Ethyl acetate soluble portion and compounds 13 and 7 were the most potent samples with the IC₅₀ value of 41.25 μg/mL, 36.76 μM, and 13.16 μM against MCF-7, HeLa, and A549 cell lines, respectively. To the best of our knowledge, this is the inaugural investigation into the chemical constituents of the genus Raphidiocystis, offering novel insights into the chemotaxonomy of this hitherto poorly understood genus.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ergostane-type steroids from mushrooms of Pleurotus genus.","authors":"Takashi Kikuchi","doi":"10.1007/s11418-024-01872-5","DOIUrl":"https://doi.org/10.1007/s11418-024-01872-5","url":null,"abstract":"<p><p>Steroids are physiologically important compounds for animals, plants, and fungi, and they have significantly contributed to drug discovery for many years. Fungi mainly biosynthesize ergostane-type steroids such as ergosterol. However, after the basic skeleton is biosynthesized, chemical transformations can lead to the cleavage or rearrangement of the fundamental skeleton of steroids. The cleaved (seco) and rearranged (abeo) steroids are chemically and pharmacologically intriguing because they often exhibit biological activities. As ergostane-type steroids including the normal, seco, and abeo types have been isolated from the mushrooms of the Pleurotus genus, this review focuses on them as a resource of ergostane-type steroids, providing their distribution, structure determination, and biological activity.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol as a BCL6 natural inhibitor suppresses germinal center derived Non-Hodgkin lymphoma cells growth.","authors":"Yajing Xing, Chunbin Tan, Zhoujiang Liu, Yanqi Liu, Simei Liu, Guixue Wang, Yadong Zhong","doi":"10.1007/s11418-024-01873-4","DOIUrl":"https://doi.org/10.1007/s11418-024-01873-4","url":null,"abstract":"<p><p>Non-Hodgkin lymphomas (NHL), including diffuse large B-cell lymphoma (DLBCL), Burkitt lymphoma (BL), and follicular lymphoma (FL), predominantly arise from B cells undergoing germinal center (GC) reactions. The transcriptional repressor B-cell lymphoma 6 (BCL6) is indispensable for GC formation and contributes to lymphomagenesis via its BTB domain-mediated suppression of target genes. Dysregulation of BCL6 underpins the pathogenesis of GC-derived NHL. While pharmacological targeting the BCL6-BTB domain has shown therapeutic promise, natural product-based inhibitors remain underexplored. In this study, resveratrol, a polyphenolic compound derived from grapes, was identified as a potent BCL6 inhibitor through a comprehensive screen of traditional Chinese medicine monomers using Homogeneous Time-Resolved Fluorescence (HTRF) assay. As a BCL6 natural inhibitor, resveratrol effectively disrupted the BCL6/SMRT interaction, reactivated suppressed gene expression, and inhibited the proliferation of GC-derived NHL cells. It also exhibited synergistic efficacy when combined with EZH2 and PRMT5 inhibitors. In vivo, resveratrol suppressed GC formation, reduced follicular helper T-cell frequencies, impaired class-switch recombination, and disrupted immunoglobulin affinity maturation. Furthermore, it markedly inhibited the progression of GC-derived NHL in animal models. Our findings demonstrate that resveratrol functions as a natural BCL6 inhibitor with significant therapeutic potential for the treatment of GC-derived NHL.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic activity of C₁₇ polyacetylenes from the roots of Glehnia littoralis against drug-resistant colorectal and lung cancer cells.","authors":"Jisu Park, Sihyun Jang, Seung-On Lee, InWha Park, Sainan Li, A-Young Nam, Jung-Hyun Shim, MinKyun Na","doi":"10.1007/s11418-024-01870-7","DOIUrl":"https://doi.org/10.1007/s11418-024-01870-7","url":null,"abstract":"<p><p>In the preliminary screening, falcarinol and falcarindiol, C₁₇ polyacetylenes from the roots of Glehnia littoralis F. Schmidt ex Miq (Umbelliferae), displayed cytotoxic activity both against oxaliplatin-sensitive/resistant colorectal cancer (CRC) and gefitinib-sensitive/resistant non-small cell lung cancer (NSCLC) cells. In this study, 13 polyacetylenes including a new (3R,11R)-11-hyroxy-isofalcarinolone (1) were isolated from G. littoralis, and the chemical structures were elucidated through NMR, HRMS, and optical rotation analysis as well as DP4 plus calculation. (3S,8S)-Oplopandiol (5) showed cytotoxic activities against oxaliplatin-sensitive and -resistant CRC (HCT116 and HCT116-OxR) cells. Unexpectedly, (3R,8S)-falcarindiol (3) and (3S)-falcarinol (12), newly purified from G. littoralis, did not show cytotoxic activity. It was confirmed that both falcarinol and falcarindiol, previously evaluated, were decomposed, which seems to be the cause of inconsistent biological results. These findings indicated that C₁₇ polyacetylene could be a good starting compound for the development of anticancer leads against drug-resistant CRC and NSCLC cells, however, the stability of C₁₇ polyacetylens should be considered for further studies.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthohumol attenuates TXNIP-mediated renal tubular injury in vitro and in vivo diabetic models.","authors":"Yan Zhang, Runzhou Pan, Zhang Shou, Yongcai Zhao","doi":"10.1007/s11418-024-01863-6","DOIUrl":"https://doi.org/10.1007/s11418-024-01863-6","url":null,"abstract":"<p><p>Thioredoxin-interacting protein (TXNIP), as a pivotal protein in the cellular stress response, plays a significant role in the progression of diabetic nephropathy (DN). Consequently, therapeutic strategies aimed at targeting TXNIP may offer novel interventions for patients with DN. Our study is to explore the therapeutic potential of targeting TXNIP in mitigating renal tubular injury induced by hyperglycemia. Cell viability and apoptosis of renal tubular epithelial cells (RTECs) were evaluated using CCK-8, Annexin V/7-AAD, and TUNEL staining after exposure to normal glucose (NG; 5 mM), high glucose (HG; 30 mM), or treatment with TXNIP inhibitors (Xanthohumol, Xan). Furthermore, histochemical staining was utilized to assess the morphological changes in the kidney. Xan was determined to be a potential inhibitor of TXNIP due to its low binding energy value of - 7.433 kcal/mol. Both genetic inhibition of TXNIP using sh-RNA and pharmacological inhibition with Xan were found to reverse HG-induced RTEC apoptosis and inflammatory response. In diabetic mice, administration of Xan resulted in significant improvements in pathological features such as tubular atrophy, tubular injury score, and collagen deposition in the tubulointerstitium. Additionally, treatment with Xan effectively reduced the up-regulation of TXNIP protein expression caused by hyperglycemia. In conclusion, Xan, as a bioactive natural product, has been shown to attenuate hyperglycemia-induced renal tubular injury in both in vitro and in vivo models, potentially through the inhibition of TXNIP expression. Xan has the potential to serve as a therapeutic compound for the treatment of DN.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142919051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red ginseng prevents niraparib-induced myelosuppression in C57BL/6 mice via inhibiting p53-mediated upregulation of p21 and p27.","authors":"Huiyan Liao, Xiangdan Hu, Shenming Chen, Zhaofeng Fan, Jing Xiao","doi":"10.1007/s11418-024-01866-3","DOIUrl":"https://doi.org/10.1007/s11418-024-01866-3","url":null,"abstract":"<p><p>Myelosuppression is a serious and common complication of targeted therapy for cancer patients, and there are few studies exploring the efficacy of natural drugs in this condition. Niraparib is a widely used targeted therapy for the treatment of advanced ovarian cancer. As a poly (ADP-ribose) polymerase (PARP) inhibitor, niraparib significantly improves progression-free and overall survival in patients. We aimed to explore the potential effect of red ginseng (RG) on niraparib-induced myelosuppression and to further reveal its possible molecular mechanism. Female C57BL/6 mice were divided into control, tumor, model, and RG groups (n = 6). After receiving ID8 ovarian cancer cell inoculation, the mice received niraparib treatment (80 mg/kg) for 3 days. Meanwhile, RG groups (100 and 200 mg/kg) were intragastrically treated with RG extract for 7 days. Compared with the model group, RG extract increased the counts of peripheral blood cells and enhanced the hematopoietic function of bone marrow. Furthermore, RG extract increased the colony yield of hematopoietic progenitor cells (HPCs), facilitated DNA damage repair, alleviated the G0/G1 phase cell cycle arrest, and significantly reversed the increased expression levels of p53, p21, and p27, while stimulating cyclinE1 expression levels. These findings indicate that RG might have therapeutic potential on niraparib-induced myelosuppression, which encourages further clinical trials. This study is the first to explore the efficacy and mechanism of RG in preventing myelosuppression induced by niraparib.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten new resin glycosides, ipoalbins I-X, from Ipomoea alba seeds.","authors":"Nodoka Misuda, Mizuki Watanabe, Hirotaka Nishikawa, Shin Yasuda, Hiroyuki Miyashita, Kazumi Yokomizo, Hitoshi Yoshimitsu, Ryota Tsuchihashi, Masafumi Okawa, Junei Kinjo, Masateru Ono","doi":"10.1007/s11418-024-01868-1","DOIUrl":"https://doi.org/10.1007/s11418-024-01868-1","url":null,"abstract":"<p><p>Ipomoea alba L. (Convolvulaceae) is an annual vine native to tropical America that is cultivated primarily for ornamental purposes. Its seeds are used in traditional medicine as a laxative, and young shoots are consumed as food. In this study, ten new resin glycosides, ipoalbins I (1)-X (10), were isolated from I. alba seeds. The structures of 1-10 were determined based on spectroscopic data. All compounds had intramolecular cyclic ester structures (jalapins). The sugar moieties in 1-10 were partially acylated by organic acids, including isobutyric, (E)-2-methylbut-2-enoic, and 2S-methyl-3S-hydroxybutyric acids. Notably, one of these compounds has a rare jalapin structure characterized by an ester linkage between the carboxyl group of the aglycone moiety and the hydroxyl group of an organic acid attached to the sugar moiety. In contrast, other compounds exhibited typical macrolactone structures. Furthermore, the cytotoxic activity of 1-10 against HL-60 human promyelocytic leukemia cells and their antiviral activity against herpes simplex virus type 1 (HSV-1) were evaluated. All tested compounds, except 3, were comparable to or slightly less cytotoxic than cisplatin, the positive control. In addition, all the compounds showed anti-HSV-1 activity; notably, 5 showed an EC₅₀ value lower than that of acyclovir, the positive control. However, the selectivity indices of these compounds were lower than that of acyclovir.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing post-harvest processing conditions for Angelica acutiloba roots in Hokkaido: storage temperature and duration.","authors":"Satoko Sasaki, Satoshi Yokota, Yohei Sasaki","doi":"10.1007/s11418-024-01867-2","DOIUrl":"https://doi.org/10.1007/s11418-024-01867-2","url":null,"abstract":"<p><p>The traditional post-harvest processing method of Angelica acutiloba roots, which involves hanging the roots outdoors after being harvested, is known to promote the conversion of starch in roots into sucrose, thereby increasing sweetness. At the same time, this method increases the dilute ethanol-soluble extract (DEE) content in A. acutiloba roots to meet the standard set by the Japanese Pharmacopoeia 18th edition. However, in Hokkaido, where A. acutiloba has been cultivated in recent years, it is a challenge to practice this traditional post-harvest processing method owing to the risk of freezing. Therefore, it is necessary to determine post-harvest processing conditions to increase DEE content in A. acutiloba roots in Hokkaido. In this study, we cultivated seedlings in plug trays and the open field in Hokkaido and stored the harvested products at various temperatures and durations to determine changes in DEE content. DEE content immediately after harvest was 20.5% in roots from plug-tray seedlings and 27.8% in roots from seedlings cultivated in the open field. DEE content in roots stored at 0 °C increased slowly over 5 weeks, whereas that in roots stored at 20 °C increased rapidly. We found a strong correlation between DEE content and sucrose content, but not (Z)-ligustilide content. Our findings on the post-harvest processing conditions for roots revealed that the optimal post-harvest processing conditions are storage at 0 °C for at least two weeks during which DEE content increases stably, similar to the traditional method.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ellagic acid prevents ovariectomy-induced bone loss and attenuates oxidative damage of osteoblasts by activating SIRT1.","authors":"Liwei Guo, Pengcheng Wei, Shijie Li, Lulu Zhou, Yunjie Yan, Duan Li","doi":"10.1007/s11418-024-01859-2","DOIUrl":"https://doi.org/10.1007/s11418-024-01859-2","url":null,"abstract":"<p><p>Oxidative stress has been implicated as a causative factor for the development and progression of osteoporosis(OP). Ellagic acid (EA), a natural polyphenol, presents anti-oxidative and anti-inflammatory properties. However, EA's role and molecular mechanism in osteoblasts have not yet been elucidated. In this study, exogenous supplementation with EA restored the osteoporotic bone defects in ovariectomized (OVX)-induced osteoporotic mice. Also, EA inhibited the H₂O₂-induced apoptosis of primary osteoblasts, prevented the production of reactive oxygen species, and restored the bone-forming potential of osteoblasts. Furthermore, EA was revealed to activate Sirtuin1 (SIRT1) and its downstream Nrf2/Heme Oxygenase 1 (HO-1) signaling pathway, and EX527 (a SIRT1 inhibitor) partially counteracted the effect of EA on bone loss. The findings suggest that EA protects against osteoporotic bone loss by activating SIRT1 and its downstream Nrf2/HO-1 signaling pathway, providing novel insights into the potential of EA as a treatment agent for osteoporosis-related bone metabolism diseases.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Huanglian decoction prevents and treats radiation-induced intestinal injury in lung cancer by regulating endoplasmic reticulum stress","authors":"Liang-liang Shi,&nbsp;Ya-ping Chen,&nbsp;Hao-ming Guo,&nbsp;Xue-peng Wang,&nbsp;Yang-yang Li,&nbsp;Shuai-zhe Wang,&nbsp;Shang-zu Zhang,&nbsp;Qi-hong Zhuo,&nbsp;Yong-qi Liu,&nbsp;Ben-jun Wei,&nbsp;Li-ying Zhang","doi":"10.1007/s11418-024-01860-9","DOIUrl":"10.1007/s11418-024-01860-9","url":null,"abstract":"<div><p>Radiotherapy is a primary treatment method for lung cancer, and radiation-induced lung injury has been widely studied. However, the lung and intestines are closely related, and patients receiving lung radiotherapy often experience gastrointestinal reactions. Huanglian Decoction has proven effective in treating intestinal diseases, but its role in radiation-induced intestinal injury in lung cancer has not yet been demonstrated. The study investigated the potential protective mechanisms of Huanglian Decoction against radiation-induced intestinal injury in lung cancer. In vivo experiments were conducted to examine the morphological changes. Changes in endoplasmic reticulum stress-related proteins were assessed using electron microscopy, immunohistochemistry, immunofluorescence, and Western blotting. In vitro studies involved the overexpression of TRPA1 protein in NCM460 cells via lentiviral vectors. Tumor-bearing mice exhibited severe damage to both lung and colon tissues following radiotherapy, with elevated levels of IL-33, increased expression of ST2L and TRPA1 in colon tissues, higher levels of endoplasmic reticulum stress-related proteins, and the presence of apoptosis and inflammatory responses. Huanglian Decoction reduced radiation-induced intestinal injury by lowering IL-33 levels, which in turn reduced endoplasmic reticulum stress response in colon tissues. In TRPA1-overexpressing NCM460 cells, Huanglian Decoction decreased TRPA1 expression levels and significantly alleviated endoplasmic reticulum stress response. Conclusively, the results indicate that Huanglian Decoction alleviates radiation-induced intestinal endoplasmic reticulum stress by reducing IL-33 levels, subsequently inhibiting the expression of ST2L and TRPA1 in colon tissues. This demonstrates the protective effect of Huanglian Decoction on the intestines during lung cancer radiotherapy, highlighting its promising clinical application in radiation protection.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"79 1","pages":"143 - 161"},"PeriodicalIF":2.5,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}