Journal of Natural Medicines最新文献

{"title":"Exploration of bioactive natural products from tropical plant genetic resources: from diversity to functional seeds.","authors":"Hiroshi Morita, Yusuke Hirasawa, Toshio Kaneda","doi":"10.1007/s11418-026-02085-8","DOIUrl":"https://doi.org/10.1007/s11418-026-02085-8","url":null,"abstract":"<p><p>Natural products derived from higher plants continue to represent an important source of structurally diverse and biologically active molecules for drug discovery. In particular, tropical plant genetic resources provide a rich reservoir of unique secondary metabolites owing to their exceptional biodiversity. Through long-term collaborative studies conducted mainly in Southeast Asia, extensive collections of tropical plant materials were screened for diverse biological activities, leading to the discovery of numerous bioactive natural products. Early phytochemical studies on medicinal plants such as Eurycoma longifolia, a well-known traditional herbal medicine widely used in Malaysia and Indonesia, also contributed to the establishment of our research platform for tropical natural product discovery. This review summarizes representative studies on structurally unique compounds isolated from tropical plant resources and highlights their biological activities and mechanisms of action. Representative examples include ceramicines, limonoids from Chisocheton ceramicus with anti-lipid droplet accumulation activity, calofolic acids from Calophyllum scriblitifolium exhibiting vasorelaxant effects through PI3K-PKA signaling modulation, cassiarins, alkaloids from Cassia siamea with potent antimalarial activity, bisindole alkaloids from Leuconotis species inducing autophagy through AKT-mTOR pathway inhibition, cyclolinopeptides from flaxseed suppressing osteoclast differentiation via the c-Fos/RANK axis, celogentins, bicyclic peptides from Celosia argentea inhibiting tubulin polymerization, and mollugin derivatives from Rubia species targeting TLR4-mediated inflammatory signaling pathways. These studies demonstrate that tropical plant-derived natural products possess remarkable structural diversity and act on biologically relevant molecular targets. Furthermore, several cases illustrate the importance of mechanistic analysis and structural modification for the optimization of pharmacological activity. Collectively, this review highlights the significance of biodiversity-driven natural product exploration as a strategy for identifying functional seeds for future drug discovery.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-MS profiling and antiulcer mechanisms of Rumex pictus extract and its isolated emodin-8-O-β-glucoside against ethanol-induced gastric damage.","authors":"Enaam M AbouZeid, Lamia T Abou El-Kassem, Sally A El Awdan, Usama W Hawas, Nagwa M Ammar","doi":"10.1007/s11418-026-02091-w","DOIUrl":"https://doi.org/10.1007/s11418-026-02091-w","url":null,"abstract":"<p><p>Peptic ulcer disease is considered a global health concern, commonly caused by Helicobacter pylori infection, chronic NSAID use, and ethanol-induced gastric mucosal injury. Although proton pump inhibitors and anti-secretory drugs are effective, their prolonged use increases risks of fractures, infections, and nutrient deficiencies, highlighting the urgent need for safer alternatives. The Rumex genus, particularly R. pictus Forssk. aerial parts, has long been employed in Middle Eastern traditional medicine to alleviate gastric inflammation and ulcers. The antiulcer bioassays of the 70% aqueous methanolic R. pictus extract (RPE) (150-300 mg/kg) and its isolated emodin-8-O-β-glucoside (EGL) (15-30 mg/kg) were assessed using an in vivo ethanol-induced gastric injury model. Additionally, LC-ESI-MS/MS was used to identify the phenolic constituents from RPE. 42 Metabolites were identified from LC-ESI-MS/MS profiling of RPE including phenolic acids (e.g. caffeic, ferulic, chlorogenic), flavonoid C- and O-glycosides (e.g. orientin, isoorientin, vitexin, isovitexin, hyperoside), and anthraquinones (e.g. emodin, chrysophanol, physcion, and their glycosides). Both RPE and EGL restored gastric antioxidant defenses by elevating glutathione and glutathione-S-transferase while lowering MDA levels, downregulated pro-inflammatory cytokines: TNF-α and IL-1β, and enhanced cytoprotective PGE2 production. Notably, the higher dose of EGL (30 mg/kg) produced superior gastroprotection as it demonstrated greater suppression of oxidative stress and cytokine release alongside enhanced mucosal regeneration. Histopathological analysis further confirmed reduced hemorrhage, edema, and epithelial necrosis, particularly at higher doses. These findings establish RPE and EGL as promising gastroprotective candidates, reinforcing their ethnomedicinal value and supporting further development as safer alternatives for peptic ulcer management.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a species-specific PCR-lateral flow assay integrated with SYBR Safe for rapid authentication of commercial Polygoni Multiflori Caulis (Ye-Jiao-Teng).","authors":"Ngoc-Phuoc Ngo, Yen-Ying Kung, PeiJung Chiang, Meng-Shiou Lee, Kai-Wei Chang, Kun-Chang Wu","doi":"10.1007/s11418-026-02068-9","DOIUrl":"https://doi.org/10.1007/s11418-026-02068-9","url":null,"abstract":"<p><p>Pleuropterus multiflorus (PM) is a widely used medicinal plant in traditional Chinese medicine (TCM). However, the closely related species Pleuropterus angulatus (PA) is commonly misidentified as PM due to morphological similarities, leading to frequent adulteration in commercial Polygoni Multiflori Caulis (PMC) products. To address this issue, we developed a species-specific PCR-lateral flow assay integrated with SYBR Safe for rapid and species-specific authentication of PM and PA. Species-specific primers were designed based on a diagnostic single nucleotide polymorphism (SNP) within the chloroplast trnL-trnF region. After validation of specificity, visual detection of PM and PA was achieved using lateral flow strips. The assay demonstrated detection limits of 0.5 ng/μL for PM and 0.05 ng/μL for PA genomic DNA. The application of this assay to eighteen commercial PMC products revealed the presence of PA in all samples, with only one product containing both PM and PA, indicating widespread adulteration in the herbal marketplace. In conclusion, the developed PCR-lateral flow assay provides a rapid, sensitive, and user-friendly molecular diagnostic tool suitable for authenticity testing of herbal products and quality control during pharmaceutical manufacturing.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiproliferative activities of panduratin A and its derivatives against U-251 MG glioblastoma cells and U-251 MG cancer stem-like cells: structure-activity relationships and identification of PHB2 as a candidate binding protein.","authors":"Kousuke Araki, Yuna Yoshitani, Rina Okamoto, Shigekuni Hosogi, Ryota Fujikawa, Moe Kaneko, Reia Ishikura, Chiho Shimadzu, Yuzuki Takahashi, Rina Mantani, Tomoe Ohta, Seikou Nakamura","doi":"10.1007/s11418-026-02095-6","DOIUrl":"https://doi.org/10.1007/s11418-026-02095-6","url":null,"abstract":"<p><p>Panduratin A (1), a cyclohexenyl chalcone isolated from Boesenbergia rotunda, has been reported to exhibit various biological activities, including antitumor effects. However, its antiproliferative activity against glioblastoma cells and glioblastoma-derived cancer stem-like cells (CSCs), and the molecular basis of this activity, remain incompletely elucidated. In this study, we evaluated the antiproliferative activity of 1 against U-251 MG glioblastoma cells (non-CSCs) and U-251 MG CSCs and found that 1 exhibited potent activity against both cell populations. To investigate the structural features associated with this activity, cyclohexenyl chalcone derivatives of 1 (2-11) were synthesized through a chalcone intermediate generated from an acetophenone precursor, followed by Diels-Alder reactions with dienes. Structure-activity relationship analysis, particularly in U-251 MG non-CSCs, suggested that the phenolic oxygen functionalities of 1 contribute to its potent activity, whereas some acetylated derivatives retained moderate activity. Based on this observation, affinity-based target identification using U-251 MG non-CSC extracts identified prohibitin 2 (PHB2), an inner mitochondrial membrane protein, as a candidate binding protein for 1. In these cells, 1 also markedly altered the mitochondrial morphology and reduced the mitochondrial membrane potential. Together, these findings suggest that the antiproliferative activity of 1 in U-251 MG glioblastoma cells may be associated with PHB2 and mitochondrial alterations.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-derived phytochemicals for Alzheimer's disease: mechanisms of action, experimental evidence, and therapeutic perspectives.","authors":"Janhavi Nimbalkar, Rushikesh Shinde","doi":"10.1007/s11418-026-02090-x","DOIUrl":"https://doi.org/10.1007/s11418-026-02090-x","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterised by cholinergic deficits, amyloid-beta accumulation, tau hyperphosphorylation, oxidative stress, neuroinflammation, and synaptic dysfunction, which collectively contribute to cognitive decline and neuronal loss. Conventional pharmacotherapies provide symptomatic relief but largely target single pathological pathways, highlighting the need for multi-targeted approaches. Herbal therapeutics have emerged as promising complementary or alternative interventions, offering multifaceted mechanisms that simultaneously modulate key disease processes. Preclinical studies, including in vitro and animal models, indicate that extracts of medicinal plants, including Bacopa monnieri (aerial parts), Ginkgo biloba (leaves), Curcuma longa (rhizomes), and Withania somnifera (roots), may enhance cognitive function, exert neuroprotective effects, attenuate oxidative stress and inflammation, and modulate amyloid and tau pathology. However, clinical evidence remains limited, heterogeneous, and inconclusive, with existing studies reporting variable effects on memory, attention, executive function, and global cognition and generally providing only preliminary evidence of safety and efficacy. This translational gap is further influenced by poor bioavailability and brain delivery of several phytoconstituents, variability in herbal preparations, inadequate standardisation, differences in dosing and study design, and the scarcity of adequately powered, long-term clinical trials. Emerging approaches, including nanotechnology-based delivery systems, rational combinations with conventional therapeutics or complementary herbal agents, and personalised interventions based on genetic and metabolic characteristics, may improve therapeutic precision and clinical translation. Integrating mechanistic insights with experimental and clinical evidence may help identify the most promising herbal candidates while defining their translational limitations. This review synthesises current evidence and future directions, providing a framework for advancing herbal interventions in AD.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A standardized fraction of Cajanus scarabaeoides attenuates diabetic nephropathy via antiglycation, anti-inflammatory, and anti-fibrotic mechanisms.","authors":"Rahul Baghel, Arvind K Maurya, Preety Kaushik, Manisha Yadav, Ishbal Ahmad, Madhav N Mugale, Sanjeev Kanojiya, Akhilesh K Tamrakar","doi":"10.1007/s11418-026-02089-4","DOIUrl":"https://doi.org/10.1007/s11418-026-02089-4","url":null,"abstract":"<p><p>Diabetic nephropathy is the critical microvascular complication of diabetes and a leading cause of end-stage renal disease. Its pathophysiology incriminates complex and interconnected mechanisms driven by hemodynamic and metabolic alterations, accompanied with glycation, inflammation, oxidative stress, and fibrosis. Targeting these cues through natural interventions may preserve kidney functions. The study aimed to investigate the renoprotective potential of a standardized ethyl acetate fraction of C. scarabaeoides (EAFCS), enriched with flavonoids including isoorientin, orientin, vitexin, isovitexin, and genistin, in diabetic nephropathy. In vitro mechanistic studies were performed in NRK-52E rat kidney tubular epithelial cells exposed to high glucose, while in vivo efficacy was assessed in a streptozotocin-induced diabetic rats. EAFCS markedly prevented advanced glycation end product (AGE) formation at early and intermediate stages of the glycation process and also promoted the degradation of preformed AGEs. In vitro, EAFCS attenuated glucose-induced ROS generation, inflammatory response, and pro-fibrotic gene expression. In diabetic rats, EAFCS ameliorated classical diabetic manifestations, including polyphagia, polydipsia, polyuria, and impaired glucose tolerance. Also, EAFCS reduced albuminuria and proteinuria, and improved renal function markers. Histopathological and molecular analyses demonstrated that EAFCS alleviated diabetes-induced renal structural damage and fibrosis. Mechanistically, EAFCS curbed TGF-β/Smad signaling, prevented myofibroblast activation, and reduced fibronectin and collagen accumulation in renal tissue. Additionally, EAFCS downregulated RAGE expression and inhibited NF-κB- and MAPK-mediated inflammatory signaling cascades. Collectively, the findings suggest that EAFCS attenuates multiple manifestations of renal dysfunctions and may exert renoprotective effects through mechanisms associated with reduced glycation, oxidative stress, inflammation, and fibrosis. These results support EAFCS as a potential candidate for further investigation in the management of diabetic nephropathy.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracts of okra mucilage protect intestinal homeostasis through gut microbiota and immune responses.","authors":"Mengli Qing, Benyue Sun, Yuehan Li, Zhiyi Meng, Qidong Lu, Keqiang Li, Gang Chen","doi":"10.1007/s11418-026-02083-w","DOIUrl":"https://doi.org/10.1007/s11418-026-02083-w","url":null,"abstract":"<p><p>Okra pods (Abelmoschus esculentus (L.) Moench) are known as a vegetable, the mucilage of which possesses many bioactivities. Okra mucilage (OM) is a carbohydrate-rich natural extract containing polysaccharides and other bioactive components. However, its efficacy under different types of intestinal stress and its comparison with standard therapeutic agents remain unclear. Chemical characterization demonstrated that OM contains abundant carbohydrates and diverse chemical constituents. In this study, we evaluated the protective effects of OM in dextran sulfate sodium (DSS)-induced colitis and fasting-induced intestinal injury models in mice. Mesalazine and GA were used as a positive control in DSS and fasting-induced injury models, respectively. Generally, OM treatment significantly alleviated intestinal pathological damage, restored intestinal barrier integrity, promoted epithelial regeneration, and modulated gut microbial composition in these two models. In DSS-induced colitis, OM increased the abundance of short-chain fatty acid (SCFA)-producing bacterial families Muribaculaceae and Lachnospiraceae, while reducing pro-inflammation-associated Bacteroidaceae, and suppressed key inflammatory genes and factors, including Cxcl1, Cxcl2, Cxcl5, TNF, NF-κB. Notably, the regulatory effects of OM were comparable to mesalazine. In fasting-induced intestinal injury, OM restored microbial balance by regulating taxa such as Enterobacteriaceae and Erysipelotrichaceae, and downregulated gluconeogenesis-related genes including Klf15, Pck1, and Hmgcs2. OM upregulated genes involved in epithelial barrier function and immune homeostasis such as Btnl7, Btnl8, regulating pathways HIF-1, PI3K-Akt, MAPK and glycolysis/gluconeogenesis pathways. These findings demonstrate that OM alleviates intestinal injury by modulating gut microbiota, suppressing inflammation, and restoring intestinal immune and metabolic homeostasis, highlighting its potential as a natural mucilage-derived ingredient for maintaining intestinal homeostasis.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The endophyte secondary metabolite phenazine-1-carboxylic acid promotes tanshinone biosynthesis in Salvia miltiorrhiza callus.","authors":"Miho Hamai, Anju Ishii, Nozomi Sakamoto, Hiroki Fujioka, Junko Tsukioka, Tomoe Ohta, Seikou Nakamura, Takahiro Matsumoto","doi":"10.1007/s11418-026-02075-w","DOIUrl":"https://doi.org/10.1007/s11418-026-02075-w","url":null,"abstract":"<p><p>Salvia miltiorrhiza is a traditional medicinal plant widely used to treat cardiovascular and cerebrovascular diseases. Tanshinones, including cryptotanshinone, a major bioactive constituent, exhibit various pharmacological activities. Therefore, methods for efficiently increasing tanshinone production are urgently needed. Recently, plant endophytes have attracted considerable attention as novel elicitors that promote the production of valuable bioactive compounds. In the present study, we investigated the effects of secondary metabolites produced by endophytes on the biosynthesis and accumulation of tanshinones in S. miltiorrhiza. Pseudomonas chlororaphis KPUPH-SMC was identified as an endophyte capable of promoting tanshinone production, and phenazine-1-carboxylic acid (PCA) was identified as the metabolite responsible for this activity. Our transcriptome analysis further demonstrated that PCA significantly upregulated the gene expression of tanshinone biosynthetic enzymes (GGPS, CPS, and KSL1). These findings suggest that endophyte-derived secondary metabolites can be used to enhance the biosynthesis of valuable compounds in medicinal plants. This approach represents a promising strategy to improve the quality of herbal medicines.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ganoderma lucidum polysaccharide protects against myocardial ischemic injury by restoring myocardial energy metabolism and alleviating oxidative stress in mice and AC16 cardiomyocytes.","authors":"Xiao-Ting Zhang, Ming-Qiao Hu, Shi-Yu Wu, Jiang-Ying Feng, Xu Xu, Zhong-Yuan Gan, Jian-Ping Gao","doi":"10.1007/s11418-026-02093-8","DOIUrl":"https://doi.org/10.1007/s11418-026-02093-8","url":null,"abstract":"<p><p>Myocardial ischemic injury is characterized by impaired energy metabolism and oxidative stress, which contribute to cardiac dysfunction and disease progression. Ganoderma lucidum polysaccharide (GLP), a major bioactive component of Ganoderma lucidum, exhibits various pharmacological activities; however, its protective effects against myocardial ischemic injury and underlying mechanisms remain unclear. This study investigated the effects of GLP against isoproterenol (ISO)-induced myocardial ischemic injury and its involvement in metabolic regulation and oxidative stress. A mouse myocardial ischemic injury model was established by subcutaneous injection of ISO (2 mg/kg/day) for 11days. Mice were randomly divided into normal control, model, GLP treatment groups with GLP administered orally at 50 and 100 mg/kg/day, and at 50 mg/kg/day by intraperitoneal injection. An ISO-induced injury model in vitro was established using AC16 cardiomyocytes, which were pretreated with GLP (0.25 ~ 1.00 µg/mL) followed by ISO exposure (40 µM, 24 h). GLP significantly alleviated ISO-induced myocardial injury, reduced cardiac hypertrophy, improved histopathological alterations, and decreased serum AST and LDH activities in mice. Similar protective effects were observed in AC16 cells. Moreover, GLP restored myocardial energy metabolic homeostasis, by improving ATP, phosphocreatine, creatine, and total adenine nucleotides levels, while reducing cAMP accumulation. GLP also alleviated oxidative stress by decreasing malondialdehyde level and enhancing total superoxide dismutase activity. Furthermore, GLP increased the expression of AMPK-α2, phosphorylated AMPK-α2, PGC-1α, and PPAR-α in myocardial tissue. These findings suggest that GLP protects against ISO-induced myocardial ischemic injury by improving myocardial energy metabolism and reducing oxidative stress, with AMPK/PGC-1α/PPAR-α-related metabolic regulation potentially contributing to its cardioprotective effects.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new phenolic glycosides from root bark of Periploca sepium and their hypoglycemic activity.","authors":"Cai-Lian Fan, Hang-Hang Wang, Zhi-Heng Shu, Wei-Wu Chen","doi":"10.1007/s11418-026-02088-5","DOIUrl":"https://doi.org/10.1007/s11418-026-02088-5","url":null,"abstract":"<p><p>Three new phenolic glycosides (1-3) and four known compounds were isolated from the ethanol extract of the root bark of Periploca sepium. Their chemical structures were elucidated based on comprehensive spectroscopic analyses, including high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), 1D and 2D nuclear magnetic resonance (NMR) spectra. The sugar moieties were determined via acid hydrolysis followed by comparison with standard sugars. The hypoglycemic activity was evaluated by α-glucosidase and α-amylase inhibition assays. All four phenolic glycosides exhibited inhibitory activity against α-glucosidase and α-amylase with IC₅₀ values below 100 µM. Among them, compound 1 exhibited the strongest α-glucosidase inhibitory activity with an IC₅₀ value of 17.25 ± 1.39 µM, which was significantly better than that of the positive control acarbose (IC₅₀ = 111.2 ± 0.35 µM). Meanwhile, compound 2 showed the most potent α-amylase inhibitory activity with an IC₅₀ value of 30.99 ± 0.92 µM, also superior to acarbose (IC₅₀ = 149.63 ± 0.64 µM). Molecular docking results indicated that compounds 1-4 had strong binding affinity with α-glucosidase and α-amylase (ΔG < -6 kcal/mol), which further demonstrated that the four phenolic glycosides from P. sepium exerted their inhibitory effects on the two key enzymes through stable molecular binding interactions. These results suggested that the phenolic glycosides from P. sepium could be served as potential lead compounds for the development of anti-diabetic agents.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}