Journal of Natural Medicines最新文献_第2页

Ginsenoside Rd promotes the expression of acetylated α-tubulin and FOXJ1 in human bronchial epithelial cells. 人参皂苷Rd促进人支气管上皮细胞乙酰化α-微管蛋白和FOXJ1的表达。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-23 DOI: 10.1007/s11418-026-02084-9

Kouta Noriyama, Seikou Nakamura, Nobuyo Tamiya, Yuki Toda, Shigekuni Hosogi, Eishi Ashihara

{"title":"Ginsenoside Rd promotes the expression of acetylated α-tubulin and FOXJ1 in human bronchial epithelial cells.","authors":"Kouta Noriyama, Seikou Nakamura, Nobuyo Tamiya, Yuki Toda, Shigekuni Hosogi, Eishi Ashihara","doi":"10.1007/s11418-026-02084-9","DOIUrl":"https://doi.org/10.1007/s11418-026-02084-9","url":null,"abstract":"Mucociliary clearance is an airway defense mechanism driven by the coordinated function of mucus and motile cilia. Although ciliary beat frequency (CBF) reflects the motile function of ciliated cells, the generation and maintenance of ciliated cells are also essential for sustained airway epithelial defense. Although short-term treatment with ginsenoside Rd (Rd) increases CBF, its long-term effects on the differentiation of human airway epithelial cells remain unclear. We investigated the effects of Rd on primary normal human bronchial epithelial cells cultured under air-liquid interface conditions. On day 10, Rd increased the percentage of acetylated α-tubulin (Ac-α-tubulin)-positive cells, whereas the percentage of MUC5AC-positive cells remained unchanged. Rd promoted the time-dependent increase in Ac-α-tubulin-positive cells, while LDH release or total cell number were not significantly altered. Long-term Rd treatment did not affect baseline CBF. Furthermore, perfusion with Rd increased CBF independent of differentiation conditions. These observations suggest that the increase in Ac-α-tubulin-positive cells by long-term Rd treatment and the increase in CBF by short-term Rd treatment may be functionally separable. The increase in the percentage of Ac-α-tubulin-positive cells was suppressed by ERβ inhibitors and CREB-dependent transcription inhibitors. Rd also increased the expression of FOXJ1 mRNA transcripts. These findings suggest that Rd may modulate the expression of ciliated cell markers, such as acetylated α-tubulin and FOXJ1, and ciliary motility in human airway epithelial cells.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erinacerins from Hericium erinaceus: a review of their chemistry, bioactivities, and NMR characterization. 猴头菌中的猴头菌素：化学、生物活性和核磁共振表征的综述。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-21 DOI: 10.1007/s11418-026-02080-z

Ahmed Othman, Yhiya Amen, Kuniyoshi Shimizu

引用次数: 0

Ginsenoside Rk3 modulates glutamine metabolism and inhibits Th17 cell differentiation via gut microbiota regulation to ameliorate psoriasis. 人参皂苷Rk3通过调节肠道菌群调节谷氨酰胺代谢和抑制Th17细胞分化，改善银屑病。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-21 DOI: 10.1007/s11418-026-02073-y

Yuan Liu, Xiaoqi Wang, Liang Shi, Jiani Jiang, Zhiguang Duan, Chenhui Zhu, Rongzhan Fu

{"title":"Ginsenoside Rk3 modulates glutamine metabolism and inhibits Th17 cell differentiation via gut microbiota regulation to ameliorate psoriasis.","authors":"Yuan Liu, Xiaoqi Wang, Liang Shi, Jiani Jiang, Zhiguang Duan, Chenhui Zhu, Rongzhan Fu","doi":"10.1007/s11418-026-02073-y","DOIUrl":"https://doi.org/10.1007/s11418-026-02073-y","url":null,"abstract":"Dysbiosis of the gut microbiota has been demonstrated to trigger immune responses that may heighten psoriasis susceptibility. However, conventional therapies for psoriasis are often limited by significant side effects. Ginsenoside Rk3 (Rk3), a bioactive triterpenoid glycoside compound derived from natural ginseng, has shown promise as a potent immunomodulator and gut regulator. Therefore, this study aims to investigate the potential protective effect of Rk3 against psoriasis, with a focus on the gut microbiota modulation and immune response regulation. Network pharmacology was first employed to predict potential targets for the action of Rk3 on psoriasis and an imiquimod (IMQ)-induced psoriasis mice model was used to investigate the palliative effect of Rk3 on psoriasis. Our results reveal that Rk3 restores gut microbiota homeostasis, especially modulating the abundance of Akkermansia muciniphila and Parabacteroides distasonis, and effectively alleviates IMQ-induced psoriasis through gut microbiota-dependent mechanisms. Furthermore, Rk3 exerts a regulatory effect on aberrant amino acid metabolism, suppressing glutamine catabolism while enhancing short-chain fatty acids (SCFAs) biosynthesis. Rk3-mediated regulation of glutamine metabolism exerts an inhibition effect on the conversion of glutamine to glutamate, suppresses Th17 cell differentiation and alleviates psoriasis via the IL-6/JAK1-STAT3 signaling axis. Overall, our research elucidates Rk3's mechanism of action in ameliorating psoriasis and highlights its potential as a gut microbiota modulator for psoriasis treatment.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactivity-guided optimization of Gastrodia elata cultivation: integrated modulation of spawn wood length, tree species and pit size. 天麻栽培的生物活性优化：卵长、树种和坑大小的综合调节。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-21 DOI: 10.1007/s11418-026-02086-7

Caiyun Wang, Tong Liu, Min Liu, Xiangyu Zhang, Gaiyun Men, Rui Shen, Xiaodi Kou, Aihong Yang

{"title":"Bioactivity-guided optimization of Gastrodia elata cultivation: integrated modulation of spawn wood length, tree species and pit size.","authors":"Caiyun Wang, Tong Liu, Min Liu, Xiangyu Zhang, Gaiyun Men, Rui Shen, Xiaodi Kou, Aihong Yang","doi":"10.1007/s11418-026-02086-7","DOIUrl":"https://doi.org/10.1007/s11418-026-02086-7","url":null,"abstract":"Gastrodia elata serves as a natural product source for treating neuroinflammation and neurodegenerative diseases, yet inconsistent planting practices cause significant variations in its therapeutic components. This study investigated how spawn wood length, tree species and pit size influenced yield and bioactive compound accumulation. Bioactive compounds were quantified by HPLC, and anti-neuroinflammatory activities of major components were validated in LPS-induced BV2 microglial cells. The highest total and graded yields were achieved with 15 cm spawn wood length. Birch and cherry species yielded significantly higher production, with birch showing the highest accumulation of parishin A and B. The 0.4 m2 pit size resulted in the highest total yield and contents of parishin A and B, while maintaining relatively high levels of gastrodin and 4-hydroxybenzyl alcohol. Consistent with previous reports, parishin A and B exhibited potent anti-neuroinflammatory activities, with IC50 values below 0.1 μM for NO inhibition, surpassing gastrodin (2.30 μM) and 4-hydroxybenzyl alcohol (0.65 μM). By integrating planting conditions, yield, multi-component contents, and anti-inflammatory activities, a bioactivity-guided cultivation optimization of G. elata was explored to identify the most effective cultivation parameters. The results showed that it could simultaneously enhance both yield and enrichment of anti-neuroinflammatory components, positioning G. elata as a promising natural product source of molecules with enhanced anti-neuroinflammatory potential.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemp seed oil ameliorates DSS-induced ulcerative colitis in mice by altering transcriptomics and metabolomics. 大麻籽油通过改变转录组学和代谢组学改善dss诱导的小鼠溃疡性结肠炎。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-21 DOI: 10.1007/s11418-026-02082-x

Yujie Zhang, Yiyun Guan, Junjie Zhou, Jing Yang, Hailong Lu, Lixi Li, Zhongjie Zou, Mengjuan Gong

{"title":"Hemp seed oil ameliorates DSS-induced ulcerative colitis in mice by altering transcriptomics and metabolomics.","authors":"Yujie Zhang, Yiyun Guan, Junjie Zhou, Jing Yang, Hailong Lu, Lixi Li, Zhongjie Zou, Mengjuan Gong","doi":"10.1007/s11418-026-02082-x","DOIUrl":"https://doi.org/10.1007/s11418-026-02082-x","url":null,"abstract":"Ulcerative colitis (UC) is a chronic relapsing inflammatory bowel disease with complex pathogenesis and limited treatments. Hemp seed oil, with its unique ω-6/ω-3 polyunsaturated fatty acid ratio and phenolic antioxidants, exhibits anti-inflammatory and gut barrier-enhancing properties, though its mechanisms in UC are not fully understood. Here, we applied an integrated transcriptomics and metabolomics approach to investigate the effects of hemp seed oil in dextran sulfate sodium (DSS)-induced UC mice. Treatment with hemp seed oil (1 g/kg) significantly alleviated disease activity, suppressed proinflammatory cytokine levels (interleukin (IL)-6, IL-1β, and IL-17), and improved colonic histopathology. Metabolomic profiling via ultrahigh-performance liquid chromatography-quadrupole-orbitrap tandem mass spectrometry (UPLC-Q-Orbitrap MS/MS) identified 19 significantly altered colonic metabolites, predominantly involved in amino acid metabolism, energy homeostasis, and lipid signalling pathways. Transcriptomic analysis suggested that hemp seed oil treatment was associated with inhibition of the IL-17 and tumor necrosis factor (TNF) signalling pathways while upregulating genes involved in barrier function (e.g., zonula occludens-1 (ZO-1) and Occludin). Integrative analysis revealed strong inverse correlations between key metabolites (e.g., docosahexaenoic acid ethyl ester, adenosine diphosphate (ADP)) and IL-17 pathway activation (IL17A, C-X-C motif chemokine ligand 10 (CXCL10)), suggesting that hemp seed oil may attenuate UC through reprogramming the \"metabolism‒inflammation\" axis. These findings suggest potential multitarget therapeutic mechanisms of hemp seed oil against UC, indicate critical links between metabolic reprogramming and the immune‒inflammatory axis, and offer novel insights for the development of phytomedicine for the treatment of inflammatory bowel disease.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ceramaximin A and B, two bromotyrosine derivatives from the worm snail Ceraesignum maximum and their cytotoxic activity. 两种溴酪氨酸衍生物陶酰胺A和陶酰胺B及其细胞毒活性。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-21 DOI: 10.1007/s11418-026-02087-6

Pham Thanh Binh, Kieu Thi Phuong Linh, Vu Thanh Trung, Pham Thi Mai Huong, Le Ba Vinh, Nguyen Phuong Thao, Nguyen Chi Mai, Tran My Linh, Nguyen Van Thanh

引用次数: 0

Rhizobial inoculation promotes glycyrrhizic acid production through jasmonic acid signaling in Glycyrrhiza uralensis. 根瘤菌接种通过甘草中茉莉酸信号传导促进甘草酸的产生。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-15 DOI: 10.1007/s11418-026-02072-z

Shion Yamamoto, Aya Shimomura, Satoshi Watanabe, Nazmul Hasan, Akihiro Suzuki

{"title":"Rhizobial inoculation promotes glycyrrhizic acid production through jasmonic acid signaling in Glycyrrhiza uralensis.","authors":"Shion Yamamoto, Aya Shimomura, Satoshi Watanabe, Nazmul Hasan, Akihiro Suzuki","doi":"10.1007/s11418-026-02072-z","DOIUrl":"https://doi.org/10.1007/s11418-026-02072-z","url":null,"abstract":"We previously found that inoculation with rhizobia tends to increase both biomass production and glycyrrhizic acid (GL) production in the medicinal plants Glycyrrhiza uralensis and G. glabra. In this study, we investigated the mechanism by which rhizobial inoculation promotes GL production. Transcriptome analysis of plants grown for 3, 6, 9, and 13 weeks after rhizobial inoculation revealed significant enrichment of GO terms related to root tissue differentiation and reorganization in inoculated plants. In addition, jasmonic acid (JA)-mediated signaling pathway, fatty acid biosynthetic process, and isoprenoid biosynthetic process were specifically enriched in inoculated plants, and these terms included Jasmonate ZIM-domain protein (JAZ)-like, MYC-related transcription factor 2 (MYC2)-like, Allene oxide cyclase (AOC)-like, Allene oxide synthase (AOS)-like, and Squalene synthase 2 (SQS2)-like genes. Time-course analysis of the expression patterns of these genes showed that JAZ-like genes were upregulated at the early growth stages in inoculated plants, whereas at 13 weeks after inoculation, expression of JAZ-like genes decreased while MYC2b and CYP88D6 expression increased. These results suggest that CYP88D6 expression may be regulated by MYC2. These results suggest that activation of JA biosynthesis and JA signaling is involved in the promotion of GL production by rhizobial inoculation, thereby supporting our previously proposed hypothesis at the transcriptome level. Further studies, including JA quantification, elucidation of transcriptional regulatory mechanisms, and functional analyses, will be necessary.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lusitropic effect of Aurantii Fructus Immaturus extract on isolated mouse ventricular myocardium. 枳实提取物对离体小鼠心室心肌的致肥作用。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-08 DOI: 10.1007/s11418-026-02069-8

Maika Seki, Yuna Shiota, Ayaka Konno, Kouharu Otsuki, Takashi Kikuchi, Shogo Hamaguchi, Wei Li, Iyuki Namekata, Hikaru Tanaka

{"title":"Lusitropic effect of Aurantii Fructus Immaturus extract on isolated mouse ventricular myocardium.","authors":"Maika Seki, Yuna Shiota, Ayaka Konno, Kouharu Otsuki, Takashi Kikuchi, Shogo Hamaguchi, Wei Li, Iyuki Namekata, Hikaru Tanaka","doi":"10.1007/s11418-026-02069-8","DOIUrl":"https://doi.org/10.1007/s11418-026-02069-8","url":null,"abstract":"Impaired myocardial relaxation is a key feature of diastolic dysfunction-associated heart failure, including heart failure with preserved ejection fraction (HFpEF), and the development of agents with lusitropic activity is highly desired. In the present study, we investigated the direct acute effects of the methanolic extract from Aurantii Fructus Immaturus (Kijitsu) on myocardial relaxation and explored its active constituents and underlying mechanisms. Using isolated mouse right ventricular myocardial preparations, relaxation was evaluated by measuring the 90% relaxation time (RT90). Kijitsu extract shortened RT90 in a concentration-dependent manner, with a maximal reduction of 17.2 ± 2.6%. Among the eight major compounds identified by HPLC analysis, synephrine and nobiletin each exhibited lusitropic effects when administered alone. Pharmacological investigations demonstrated that the effects of Kijitsu extract and synephrine were abolished by the β-adrenergic receptor antagonist propranolol and the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor cyclopiazonic acid (CPA), whereas the effect of nobiletin was abolished only by CPA. Coadministration experiments resulted in enhanced lusitropic effects partially reproducing the lusitropic effect of Kijitsu extract; the lusitropic efficacy was in the order of synephrine alone < synephrine + nobiletin < synephrine + flavonoids. Furthermore, Kijitsu extract significantly improved impaired relaxation in diabetic mice exhibiting diastolic dysfunction. These findings suggest that Kijitsu extract promotes myocardial relaxation through cooperative activation of the intracellular cAMP-SERCA pathway mediated by synephrine and flavonoids, providing novel evidence that natural compounds can modulate myocardial relaxation.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyrolappaconitine, one new alkaloid isolated from sand-fried Aconitum sinomontanum Nakai with less toxicity and analgesic activity. 从沙煎乌头中分离得到的一种新生物碱——鹿蹄草碱，具有较低的毒性和镇痛作用。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-08 DOI: 10.1007/s11418-026-02081-y

Jiajia Chen, Qingyang Zheng, Yujie Wang, Wenhao Yu, Jiaying Zheng

{"title":"Pyrolappaconitine, one new alkaloid isolated from sand-fried Aconitum sinomontanum Nakai with less toxicity and analgesic activity.","authors":"Jiajia Chen, Qingyang Zheng, Yujie Wang, Wenhao Yu, Jiaying Zheng","doi":"10.1007/s11418-026-02081-y","DOIUrl":"https://doi.org/10.1007/s11418-026-02081-y","url":null,"abstract":"Aconitum sinomontanum Nakai is a traditional Chinese medicinal herb whose alkaloids serve as both its analgesic constituents and toxic components. Although sand-frying can reduce the toxicity of alkaloids, the chemical constituents of sand-fried A. sinomontanum have not been investigated. To address the issue, a phytochemical investigation was conducted on the sand-fried product of A. sinomontanum. One new compound (1) and ten known compounds (2-11) were isolated from the processed product using chromatographic separation techniques. The absolute configuration was further determined by single-crystal X-ray diffraction analysis for compound 1. Among the known isolates, compounds 2 and 10 were obtained from the processed product of A. sinomontanum for the first time, and the NMR data of compound 2 were fully reported. Based on structural similarities and literature precedents, reasonable hypotheses for the transformation pathways of the major alkaloids during sand-frying were proposed. The acute toxicity assay exhibited LD50 values of 239.9 mg/kg, 152.8 mg/kg, 8.1 mg/kg, 13.5 mg/kg, and 24.2 mg/kg for compounds 1, 3, 5, 6, and 8, respectively. Furthermore, pyrolappaconitine (1) demonstrated peripheral analgesic activity, with an ED50 value of 16.1 mg/kg.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical constituents of Guarea glabra: a new alkaloid, a new dihydro-α-pyrone, and antitrypanosomal pimarane-type diterpenes. 光桂的化学成分：一种新的生物碱、一种新的二氢-α-吡酮和抗锥虫的海马兰型二萜。

IF 2.6 4区医学

Journal of Natural Medicines Pub Date : 2026-09-08 DOI: 10.1007/s11418-026-02077-8

Takehiro Nishimura, Yuto Yamauchi, Naoko Yoshida, Ulises G Castillo, Karla Alas, Gabriel Cerén, Toshihiro Mita, Junko Nakajima-Shimada, Marvin J Núñez, Haruhisa Kikuchi

{"title":"Chemical constituents of Guarea glabra: a new alkaloid, a new dihydro-α-pyrone, and antitrypanosomal pimarane-type diterpenes.","authors":"Takehiro Nishimura, Yuto Yamauchi, Naoko Yoshida, Ulises G Castillo, Karla Alas, Gabriel Cerén, Toshihiro Mita, Junko Nakajima-Shimada, Marvin J Núñez, Haruhisa Kikuchi","doi":"10.1007/s11418-026-02077-8","DOIUrl":"https://doi.org/10.1007/s11418-026-02077-8","url":null,"abstract":"Chemical investigation of the methanolic extract of Guarea glabra Vahl (Meliaceae) led to the isolation of a new amide alkaloid containing a pyrrolidine moiety (1) and a dihydro-α-pyrone derivative (2), together with two known pimarane-type diterpenes (3, 4). The structures of the new compounds were elucidated using comprehensive spectroscopic analyses, including 1D and 2D NMR experiments. The absolute configuration of 2 was determined by comparing experimental and calculated electronic circular dichroism spectra. Compounds 1-4 demonstrated antitrypanosomal activity against the epimastigote form of Trypanosoma cruzi, with IC50 values ranging from 0.66 to 2.3 µM; compound 4 exhibited submicromolar activity. In the intracellular assay, compound 3 reduced the number of intracellular amastigotes at 10 µM. None of the isolated compounds showed significant antimalarial activity under the tested conditions. Compounds 1-4 exhibited favorable selectivity for T. cruzi over mammalian HT1080 cells. These findings highlight pimarane-type diterpenes as structurally distinct, sp3-rich scaffolds with favorable selectivity profiles and potential as leads for the development of new antitrypanosomal agents.","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0