中国临床实用医学最新文献_第6页

Expression and clinical significance of mTOR/p70S6K signaling pathway in breast carcinoma mTOR/p70S6K信号通路在乳腺癌中的表达及临床意义

中国临床实用医学 Pub Date : 2016-04-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2016.02.014

Haili Lao, Xia Li, Haizhen Wang, Yanhai Li

{"title":"Expression and clinical significance of mTOR/p70S6K signaling pathway in breast carcinoma","authors":"Haili Lao, Xia Li, Haizhen Wang, Yanhai Li","doi":"10.3760/CMA.J.ISSN.1673-8799.2016.02.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-8799.2016.02.014","url":null,"abstract":"Objective \u0000To investigate the expression of TOR(mammalian target of rapamycinm)/p70S6K(70 kDa ribosomal protein S6 kinase) signaling pathway in human breast carcinoma. \u0000 \u0000 \u0000Methods \u0000From January 2013 to January 2016, 40 cases of breast carcinom tissues,40 cases of paracancerous tissues,32 cases of normal breast tissues were selected in Binzhou Central Hospital Affiliated to Binzhou Medical College.RT-PCR and Immunohistochemistry were employed to determine the expression of mTOR/p70S6K signaling pathway in cancerous and paracancerous tissuess of 40 cases and normal healthy tissues of 32 cases. The relationship between expression of the genes mRNA in breast carcinom tissues and related parameters was analyzed. \u0000 \u0000 \u0000Results \u0000The expression level of mTOR was markedly higher in the breast carcinoma tissues (0.611±0.098) than in the paracancerous tissues (0.419±0.101) or normal breast tissues (0.381±0.043) .The expression of p70S6K mRNA was also higher in the breast carcinoma tissues(0.625±0.109) than in the paracancerous tissues(0.471±0.129) or normal breast tissues(0.441±0.130), with a significant difference among groups (P<0.05). There was a positive correlation between mTOR mRNA and p70S6K mRNA expressions in breast carcinom tissues (γ=0.491, P=0.019). The expression of mTOR/p70S6K signaling pathway in the breast carcinom tissues was significantly correlated with the pathological grading, lymph node metastasis, but not with the diameter, and age. \u0000 \u0000 \u0000Conclusions \u0000The mTOR/P70S6K signaling pathway is specifically activated in breast carcinom tissues. The mTOR/p70S6K signaling pathway might play an important role in the pathogenesis of breast carcinom. \u0000 \u0000 \u0000Key words: \u0000Breast carcinoma; Mammalian target of rapamycinm(mTOR); 70 kDa ribosomal protein S6 kinase(P70S6K); RT-PCR; Immunohistochemistry","PeriodicalId":64135,"journal":{"name":"中国临床实用医学","volume":"7 1","pages":"50-53"},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69974121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Survival prediction comparison of liver cancer different staging criteria on liver cancer resection 肝癌切除术后不同分期标准的生存预测比较

中国临床实用医学 Pub Date : 2016-04-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2016.02.003

P. Cui, D. Cheng, Yanmei Ma, Youfu Zhang, Yu-Shin Song

{"title":"Survival prediction comparison of liver cancer different staging criteria on liver cancer resection","authors":"P. Cui, D. Cheng, Yanmei Ma, Youfu Zhang, Yu-Shin Song","doi":"10.3760/CMA.J.ISSN.1673-8799.2016.02.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-8799.2016.02.003","url":null,"abstract":"Objective \u0000To investigate the prediction value of liver cancer different staging criteria on liver cancer resection. \u0000 \u0000 \u0000Methods \u0000Clinical data of 218 cases with advanced liver cancer in four hospitals were selected. All patients were scored by CS、CLIP、TNM staging and OKUDA. Evaluated this four staging systems on survival prediction value of 6 month advanced liver cancer. COX proportional hazard model was used to eliminate likelihood estimator in order to judge which staging criteria had better prognosis prediction. \u0000 \u0000 \u0000Results \u0000Follow-up period of 218 cases was 2-36 month, average follow-up time was (22.34±5.89) month, three-year survival rate was 33.94%, median survival time was 25.38 month. Of the four staging methods, degree of distinction was better TNM staging、OKUDA staging criteria. At the mean time,homogeneity,discrimination power,gradient singularity of TNM staging and OKUDA staging criteria all had higher contributed value. \u0000 \u0000 \u0000Conclusions \u0000Application of TNM staging, OKUDA on liver cancer resection have better survival prediction value. \u0000 \u0000 \u0000Key words: \u0000Liver cancer; Staging criteria; Survival prediction; COX proportional hazard model","PeriodicalId":64135,"journal":{"name":"中国临床实用医学","volume":"7 1","pages":"6-8"},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69973536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of the location of fresh embryo transfer on pregnancy outcome 新鲜胚胎移植位置对妊娠结局的影响

中国临床实用医学 Pub Date : 2016-04-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2016.02.011

Bingxue Xu, Yujie He, Juan Ma, Yuanyunzi Wang

{"title":"The influence of the location of fresh embryo transfer on pregnancy outcome","authors":"Bingxue Xu, Yujie He, Juan Ma, Yuanyunzi Wang","doi":"10.3760/CMA.J.ISSN.1673-8799.2016.02.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-8799.2016.02.011","url":null,"abstract":"Objective \u0000To explore the effects of in vitro fertilization and embryo transfer(IVF-ET) position after abdominal ultrasound guided on the pregnancy outcome. \u0000 \u0000 \u0000Methods \u0000Retrospective analysis of 293 IVF-ET cycles in our center from September 2014 to July 2015.According to the difference of the distance from the embryo to the bottom of the uterus, transfer cycles are divided into four groups: 2.0 cm,meanwhile according to the ratio of the distance from the embryo to the bottom of the uterus and the depth of the uterine cavity, transfer cycles are also divided into four groups:<1/6、1/6~<1/3、1/3~<1/2、≥1/2, respectively analysis if there were significant differences in pregnancy outcome among the groups. \u0000 \u0000 \u0000Results \u0000There was no significant differences in the observed indexes between the four groups 2.0 cm in the 293 fresh cycles (P>0.05). While the 0.05),but with the increase of the proportion,the abortion rate was increasing, the rate of ectopic pregnancy was decreased. \u0000 \u0000 \u0000Conclusions \u0000In the process of embryo transfer, the difference of the distance from the embryo to the bottom of the uterus has no effect on the outcome of the pregnancy, and the difference of the ratio of the distance from the embryo to the bottom of the uterus and the depth of the uterine cavity influence pregnancy outcome. \u0000 \u0000 \u0000Key words: \u0000In vitro fertilization and embryo transfer; Pregnancy outcome; Ultrasonic inspection","PeriodicalId":64135,"journal":{"name":"中国临床实用医学","volume":"7 1","pages":"39-42"},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69973948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy evaluation of traditional Chinese medicine combined with sorafenib in the treatment of primary hepatocellular carcinoma in the middle and late stage 中药联合索拉非尼治疗原发性中晚期肝癌的疗效评价

中国临床实用医学 Pub Date : 2016-04-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2016.02.002

Yue Wu, Benqiang Yang, Wen-yuan Liu

{"title":"Efficacy evaluation of traditional Chinese medicine combined with sorafenib in the treatment of primary hepatocellular carcinoma in the middle and late stage","authors":"Yue Wu, Benqiang Yang, Wen-yuan Liu","doi":"10.3760/CMA.J.ISSN.1673-8799.2016.02.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-8799.2016.02.002","url":null,"abstract":"Objective \u0000Evaluation of the treatment effects between sorafenib soley and by combination of SLFN and Chinese Medicine on mid to later phase hepatic carcinomas patients. \u0000 \u0000 \u0000Methods \u0000Take 48 mid to later phase hepatic carcinomas patients of our hospital from 2010 to 2015. Randomly assign 24 patients to sorafenib group and 24 to sorafenib combine Chinese medicine group. To compare the change of their liver function and multilayer spiral MSCT image. \u0000 \u0000 \u0000Results \u0000There were no significant statistical difference between the two groups on size of the tumors, liver diffusion outside, cancer embolus of portal vein(P>0.05). But there were meaningful statistical difference on TBIL, DBIL, ALT, ALB and GLB(P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Compare to chemotherapy only method, there are no different results on tumor growth and diffusion by combing with Chinese Medicine with sorafenib, but there are some effect on improving liver function and life quality. \u0000 \u0000 \u0000Key words: \u0000Primary hepatocellular carcinoma; Chinese medicine treatment; Multi-layer spiral CT","PeriodicalId":64135,"journal":{"name":"中国临床实用医学","volume":"7 1","pages":"3-5"},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69973429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia and serum deprivation protected MiaPaCa-2 cells from KAI1-induced apoptosis through autophagy pathway activation in solid tumors 在实体瘤中，缺氧和血清剥夺通过激活自噬途径保护MiaPaCa-2细胞免受kai1诱导的凋亡

中国临床实用医学 Pub Date : 2016-04-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2016.02.005

Chunyan Wu

{"title":"Hypoxia and serum deprivation protected MiaPaCa-2 cells from KAI1-induced apoptosis through autophagy pathway activation in solid tumors","authors":"Chunyan Wu","doi":"10.3760/CMA.J.ISSN.1673-8799.2016.02.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-8799.2016.02.005","url":null,"abstract":"Objective \u0000KAI1 closely correlates with pancreatic cancer metastasis. There might be some factors that protect the cells from a proliferation inhibition by KAI1 in the solid tumors' microenvironment. Hypoxia and ischemia are the main characteristics of the microenvironment within solid tumors. Whether they affect the KAI1 inhibitory effects on cell proliferation is still unclear. \u0000 \u0000 \u0000Methods \u0000MiaPaCa-2 human pancreatic cancer cells do not express KAI1 protein. However, after being infected with Ad5-KAI1, they expressed KAI1 protein. We cultured them under hypoxic and serum-free conditions to simulate the solid tumor hypoxic-ischemic microenvironment. The cells were divided into the control, hypoxic, serum-free, and hypoxic with serum-free groups. The apoptosis were observed Annexin V-FITC/PI. The green fluorescent protein-labeled light chain 3 association with autophagosome membranes was detected by confocal microscopy. The ratio of LC3-Iito LC3-I expression level was detected by western blot. Pre-treatment of 3-MA was used to inhibit the autophagy. We, then observed whether the hypoxic and serum-free condi-tions could change the effect of KAI1 on cell survival and whether the pretreatment of 3-MA could inhibit the effect of hypoxic and serum-free conditions on KAI1 function. \u0000 \u0000 \u0000Results \u0000Hypoxia and serum-free media effectively reduced the apoptosis and proliferation inhibition caused by KAI1 and was beneficial to the cell survival. 3-MA pretreatment effectively blocked the protective effect of hypoxia and serum-free media on the cells by autophagy block. \u0000 \u0000 \u0000Conclusion \u0000Serum free media and hypoxia protected the MiaPaCa-2 cells from a KAI1-induced apoptosis via autophagy induction. \u0000 \u0000 \u0000Key words: \u0000KAI1; Human pancreatic cancer; Hypoxia; Serum deprivation; Autophagy","PeriodicalId":64135,"journal":{"name":"中国临床实用医学","volume":"7 1","pages":"13-17"},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69973555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of pemirolast potassium on serum IL-4, IL-12 content in allergic rhinitis patients 培米司特钾对变应性鼻炎患者血清IL-4、IL-12含量的影响

中国临床实用医学 Pub Date : 2016-04-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2016.02.013

R. Chai, K. Ning, Jing Liu, Hua Xie, Xiaoping Lin

{"title":"Effect of pemirolast potassium on serum IL-4, IL-12 content in allergic rhinitis patients","authors":"R. Chai, K. Ning, Jing Liu, Hua Xie, Xiaoping Lin","doi":"10.3760/CMA.J.ISSN.1673-8799.2016.02.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-8799.2016.02.013","url":null,"abstract":"Objective \u0000To study the influence of decoction cytokines in allergic rhinitis patients,and explore the therapeutic mechanism. \u0000 \u0000 \u0000Methods \u0000120 person wererandomized into 3 groups,40 allergic rhinitis (AR)group,40normal control group,40 Pemirolast potassium tablets group.The serum levels of IL-4 and IL-12 were determined by ELISA methods. \u0000 \u0000 \u0000Results \u0000Pemirolast potassium serum level of IL-4 in Pemirolast potassium tablets decoction group significantly decreased compared with that of AR group (68.265±0.542) vs (38.547±1.065) (P<0.01),and the serum level of IL-12 in Pemirolast potassium tablets decoction group significantly increased (9.872±1.514) vs (15.245±1.598) (P<0.01).There is a positive correlation between them. \u0000 \u0000 \u0000Conclusions \u0000The therapeutic mechanism of Pemirolast potassium tablets decoction on allergic rhinitis is decreasing the level of IL-4 and increasing the level of IL-12, which suggests that IL-4 and IL-12 are important factors in the pathogenesis of AR. \u0000 \u0000 \u0000Key words: \u0000Pemirolast potassium tablets; Allergic rhinitis; Interleukin","PeriodicalId":64135,"journal":{"name":"中国临床实用医学","volume":"7 1","pages":"47-49"},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69974076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prognostic value of serum creatinine levels and blood lactic acid on predicting patients with severe acute pancreatitis 血清肌酐和血乳酸水平对预测重症急性胰腺炎的预后价值

中国临床实用医学 Pub Date : 2014-01-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2014.01.018

Mingli Luo, Z. Jiao, Yilin Liu, Li Li

引用次数: 0

Management of obesity related hypertension 肥胖相关高血压的管理

中国临床实用医学 Pub Date : 2014-01-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2014.01.003

Hongyu Wang

引用次数: 0

Comparison of laparoscopic and laparotomy for early detection and application value in blunt abdominal trauma 腹腔镜与剖腹手术对钝性腹部创伤早期发现的比较及应用价值

中国临床实用医学 Pub Date : 2014-01-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2014.01.022

Zheng Lin, Binsheng Lin

{"title":"Comparison of laparoscopic and laparotomy for early detection and application value in blunt abdominal trauma","authors":"Zheng Lin, Binsheng Lin","doi":"10.3760/CMA.J.ISSN.1673-8799.2014.01.022","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-8799.2014.01.022","url":null,"abstract":"Objective To compare in blunt abdominal trauma,the application value of laparoscopic early detection and traditional laparotomy compared.Methods from 2007 March-2012 year in October at the Institute for 96 cases of blunt abdominal trauma patients,divided into laparoscopy group and laparotomy group,to observe the postoperative recovery time,ECG monitoring time oxygen inhalation time,postoperative complications and mortality.Results laparoscopic exploration group of patients postoperative ECG monitoring time,compared with the control group,(ECG monitoring time)t=17.506 (P＜0.01).(continuous oxygen time) t=5.582 (P＜0.01),postoperative recovery time compared to the control group,t=14.756 (P＜0.01),with significant statistical difference significance.The postoperative complications compared with those in control group (P＜0.05 x2=7.253).The laparoscopic group died in 10％,while the control group patients died after operation in 14 cases (30.42％),x2=4.608,P＜0.05.Conclusion laparoscopy operation early interventional therapy can effectively reduce the mortality of patients after operation,complications,time and ECG monitoring time recovery to improve the postoperative patients,monitoring time,reduce the economic burden of the patients,improve the quality of life. \u0000 \u0000Key words: \u0000Blunt abdominal trauma; Laparoscopy; laparotomy","PeriodicalId":64135,"journal":{"name":"中国临床实用医学","volume":"5 1","pages":"49-50"},"PeriodicalIF":0.0,"publicationDate":"2014-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69973449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

阴式子宫肌瘤剔除术的临床观察及护理阴式子宫肌瘤剔除术的临床观察及护理

中国临床实用医学 Pub Date : 2014-01-25 DOI: 10.3760/CMA.J.ISSN.1673-8799.2014.01.040

吴如红

引用次数: 0