生物物理化学(英文)最新文献_第6页

Phosphoprotein Phosphatase 1 Isoforms Alpha and Gamma Respond Differently to Prodigiosin Treatment and Present Alternative Kinase Targets in Melanoma Cells 在黑色素瘤细胞中，磷酸化磷酸酶1亚型α和γ对Prodigiosin治疗的反应不同，并提出了替代激酶靶点

生物物理化学(英文) Pub Date : 2014-04-23 DOI: 10.4236/JBPC.2014.52008

M. Fardilha, J. Figueiredo, M. Espona-Fiedler, Juliana Felgueiras, L. Korrodi-Gregório, S. Esteves, S. Rebelo, O. D. C. E. Silva, E. F. C. E. Silva, R. Pérez-Tomás

{"title":"Phosphoprotein Phosphatase 1 Isoforms Alpha and Gamma Respond Differently to Prodigiosin Treatment and Present Alternative Kinase Targets in Melanoma Cells","authors":"M. Fardilha, J. Figueiredo, M. Espona-Fiedler, Juliana Felgueiras, L. Korrodi-Gregório, S. Esteves, S. Rebelo, O. D. C. E. Silva, E. F. C. E. Silva, R. Pérez-Tomás","doi":"10.4236/JBPC.2014.52008","DOIUrl":"https://doi.org/10.4236/JBPC.2014.52008","url":null,"abstract":"Reversible protein phosphorylation is a central regulatory mechanism of cell function. Deregulation of the balanced actions of protein kinases and phosphatases has been frequently associated with several pathological conditions, including cancer. Many studies have already addressed the role of protein kinases misregulation in cancer. However, much less is known about protein phosphatases influence. Phosphoprotein Phosphatase 1 (PPP1) is one of the major serine/threonine protein phosphatases who has three catalytic isoforms: PPP1CA, PPP1CB, and PPP1CC. Its function is achieved by binding to regulatory subunits, known as PPP1-interacting proteins (PIPs), which may prefer a catalytic isoform. Also, some inhibitors/enhancers may exhibit isoform specificity. Here we show that, prodigiosin (PG), a molecule with anticancer properties, promotes the formation of PPP1CA-AKT complex and not of PPP1CC-MAPK complex. Both, AKT and MAPK, are well-known PIPs from two pathways that crosstalk and regulate melanoma cells survival. In addition, the analysis performed using surface plasmon resonance (SPR) technology indicates that PPP1 interacts with obatoclax (OBX), a drug that belongs to the same family of PG. Overall, these results suggest that PG might, at least in part, act through PPP1C/PIPs. Also, this study is pioneer in demonstrating PPP1 isoform-specific modulation by small molecules.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70902557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atherogenic Indices and HDL Particle Size as Laboratory Parameters to Evaluate Cardiovascular Risk in the Presence of Dyslipidemia 在血脂异常的情况下，动脉粥样硬化指数和高密度脂蛋白颗粒大小作为评估心血管风险的实验室参数

生物物理化学(英文) Pub Date : 2014-04-23 DOI: 10.4236/JBPC.2014.52004

Ana Paula Caires dos Santos, M. S. Vieira, D. Deus, Rogério Jorge B. de Oliveira, A. Morikawa, Roque Aras Júnior, A. Atta, F. D. Couto, R. Maranhão, R. Couto

{"title":"Atherogenic Indices and HDL Particle Size as Laboratory Parameters to Evaluate Cardiovascular Risk in the Presence of Dyslipidemia","authors":"Ana Paula Caires dos Santos, M. S. Vieira, D. Deus, Rogério Jorge B. de Oliveira, A. Morikawa, Roque Aras Júnior, A. Atta, F. D. Couto, R. Maranhão, R. Couto","doi":"10.4236/JBPC.2014.52004","DOIUrl":"https://doi.org/10.4236/JBPC.2014.52004","url":null,"abstract":"Dyslipidemia may influence enzymes and transfer proteins needed to the lipoprotein particle remodeling. Calculated indices and evaluation of lipoprotein particle size have widely been used to predict cardiovascular risk. The aim of this study was to evaluate HDL particle size and LDL particle size estimate based on TG/HDL-C as well as apoB/apoA-I ratio as possible marker and atherogenic indices, respectively, of cardiovascular disease risk in the presence of dyslipidemia. We evaluated 100 individuals of both gender, without treatment with lipid-lowering drugs, 27 normolipidemic and 73 dyslipidemic, such as isolated hypercholesterolemia (n = 16), isolated hypertriglyceridemia (n = 17), low HDL-C (n = 26) and mixed dyslipidemia (n = 14). The HDL particle size did not differ between groups. The TG/HDL-C ratio was higher in groups with isolated hypertriglyceridemia (4.2 ± 1.5), low HDL-C (5.2 ± 3.1) and mixed dyslipidemia (5.3 ± 1.6). The apoB/apoA-I ratio was increased in all groups of dyslipidemia (apoB/apoA-I > 0.5) when compared to normolipidemic (apoB/apoA-I = 0.5, p","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70902715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Gibbs-Donnan Potential as a Tool for Membrane Vesicles Polarization Gibbs-Donnan电位作为膜囊极化的工具

生物物理化学(英文) Pub Date : 2014-04-23 DOI: 10.4236/JBPC.2014.52009

Mazur Iuliia, Kosterin Sergiy, V. Tetyana, Shkrabak Oleksandr

引用次数: 1

Non-Local pH Shifts and Shared Changing Angular Velocity Magnetic Fields: Discrete Energies and the Importance of Point Durations 非局部pH位移和共享变化角速度磁场:离散能量和点持续时间的重要性

生物物理化学(英文) Pub Date : 2014-04-23 DOI: 10.4236/JBPC.2014.52006

Nicolas Rouleau, Trevor N. Carniello, M. Persinger

{"title":"Non-Local pH Shifts and Shared Changing Angular Velocity Magnetic Fields: Discrete Energies and the Importance of Point Durations","authors":"Nicolas Rouleau, Trevor N. Carniello, M. Persinger","doi":"10.4236/JBPC.2014.52006","DOIUrl":"https://doi.org/10.4236/JBPC.2014.52006","url":null,"abstract":"Macroscopic productions of “non-locality” or “excess correlations” of dynamic changes within media between two spaces could be utilized as alternative communication systems. Previous experiments have shown that injections of a weak acid within one of two volumes of spring water sharing the same patterned circular magnetic fields with changing angular accelerations separated by non-traditional (5 m) distances were associated with opposite (basic) shifts in pH within the non-injected, non-local volume. In the present experiments, employing a different technology, pairs of beakers separated by 1 m containing either 25 cc, 50 cc, or 100 cc of spring water were placed within toroids generating weak (30, 300 nT) changing acceleration magnetic fields with 1 ms, 2 ms, or 3 ms point durations or a field whose point durations changed. When a proton source (weak acid) was injected into one beaker (local) pH shifts in the other (non-local) beaker exhibit increased acidity for the 3 ms point duration but increased alkalinity for the 1 ms duration. Neither intermittent point durations nor variable point durations for the same volumes of water placed between the two magnetic field-coupled beakers exhibited significant changes from baseline. Contingent upon the point duration of the applied field, the pH shift was consistent with a fixed quantity of decreased free protons (increased pH) or increased protons (decreased pH) in the non-local beakers. The opposite directions of the pH shifts at 1 ms and 3 ms that correspond to quantitative cosmological solutions for electrons and protons suggest these results may reflect a fundamental physical process.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70902396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Genetic Variation in Salamandra infraimmaculata from Different Habitats Using Amplified Fragment Length Polymorphism 利用扩增片段长度多态性分析不同生境下小鲵的遗传变异

生物物理化学(英文) Pub Date : 2014-04-23 DOI: 10.4236/JBPC.2014.52007

G. Degani, T. Goldberg, E. Nevo

{"title":"Genetic Variation in Salamandra infraimmaculata from Different Habitats Using Amplified Fragment Length Polymorphism","authors":"G. Degani, T. Goldberg, E. Nevo","doi":"10.4236/JBPC.2014.52007","DOIUrl":"https://doi.org/10.4236/JBPC.2014.52007","url":null,"abstract":"The purpose of the present study was to examine the genetic variation in Salamandra infraimmaculata from different breeding site habitats using the Amplified Fragment Length Polymorphism (AFLP) method. The results of the dendogram from a hierarchical cluster analysis show that the grouping of S. infraimmaculata as cluster 5 differs from all the other clusters, including the St1 (Tel-Dan stream) population, which was the most predictable. Five Haplogroups (Hg) were characterized. The mean number of alleles per locus in each population (Ne) ranged from 10.566 (Sp1) to 2.720 (Po6). An average estimated heterozygosity (He) by population ranged from 0.100 (Po6) to 0.186 (St1). Population St1, a permanent breeding site where water was available all year round, exhibited the highest level of polymorphism, while population Po6, from the ephemeral breeding site, exhibited the lowest level of polymorphism. Gene flow between clusters showed that clusters 3 and 4 are sources of migrants and also receive gene flow, while clusters 1 and 2 may be a source of migrants but may not receive much gene flow. A phylogenetic analysis, based on clustering using Nei’s genetic distance, demonstrated that the Tel-Dan population is located on a separate branch within its sub-population. The conclusion of the present study shows that the genetic divergence among isolated populations is not correlated to distance but is affected by the variation of habitats.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70902454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Hemin Protects U937 Cells from Oxidative Stress via Glutathione Synthesis 血红蛋白通过谷胱甘肽合成保护U937细胞免受氧化应激

生物物理化学(英文) Pub Date : 2014-04-23 DOI: 10.4236/JBPC.2014.52003

Y. Satoh, Kyohei Oyama, K. Sakurai

{"title":"Hemin Protects U937 Cells from Oxidative Stress via Glutathione Synthesis","authors":"Y. Satoh, Kyohei Oyama, K. Sakurai","doi":"10.4236/JBPC.2014.52003","DOIUrl":"https://doi.org/10.4236/JBPC.2014.52003","url":null,"abstract":"Although heme oxygenase-1 and glutathione play important roles in the antioxidant defense system, the sharing and/or cross-talking of the HO-1 and GSH system remain poorly understood. The object of this study is to determine whether the glutathione system is involved in the antioxidant function of hemin. Hydrogen peroxide decreased the viability of the human leukemic monocyte lymphoma cell line U937. When these cells were pretreated with hemin before the addition of hydrogen peroxide, cell death was prevented. An inhibitor of heme oxygenase-1 or glutathione biosynthesis significantly abolished this protective effect of hemin. These results suggest that both heme oxygenase-1 and glutathione are involved in the protective effects of hemin against U937 cell death, which was induced by hydrogen peroxide. Hemin induced an increase in glutathione levels following the upregulation of the gene expression and protein levels of glutamate-cysteine ligase catalytic subunit. The inhibitor of heme oxigenase-1 inhibited the upregulation of glutamate-cysteine ligase catalytic subunit expression. These results suggest that hemin induces glutathione synthesis through heme oxygenase-1 activation to protect cells from hydrogen peroxide-induced oxidative stress.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70902700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Unique Conformational Behaviour of Glutamine Peptides 谷氨酰胺肽的独特构象行为

生物物理化学(英文) Pub Date : 2014-04-23 DOI: 10.4236/JBPC.2014.52005

M. Baskar, M. Shafique, F. S. Nandel

{"title":"A Unique Conformational Behaviour of Glutamine Peptides","authors":"M. Baskar, M. Shafique, F. S. Nandel","doi":"10.4236/JBPC.2014.52005","DOIUrl":"https://doi.org/10.4236/JBPC.2014.52005","url":null,"abstract":"Trinucleotide repeat expansions (CAG) lead to increase in glutamine residues and hence increase in glutamine stretch. This leads to number of neurodegenerative diseases. Therefore, the conformation of poly Q of varying chain lengths has been investigated by quantum mechanical and molecular dynamics approaches. Glutamine contains amide linkage in the side chain. It is the interaction between side chain amide linkage and the peptide bond of the backbone which dictates the conformational behaviour. Some of the glutamine residues adopt phi psi values corresponding to poly-L-proline type II structure. Not more than three glutamine residues are found to have the same set of values and hence polyglutamine is adopting random coil structure. Carbonyl-carbonyl, CH-O interactions and hydrogen bond formation involving backbone and side chain amide chain linkages are found to contribute to the stability of the adopted structure. In simulation studies due to interaction of water molecules with the amide linkages the values undergo change and this leads to weakening of carbonyl-carbonyl interactions and hydrogen bonds. The conformational behaviour of polyglutamine peptides is shown to be chain length dependent and this may provide some insight regarding the aggregation behaviour of proteins containing poly Q stretch. Possibly this is the first systematic study of the conformational behaviour of polyglutamine peptides.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70902780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geometrical criteria for left-handed twists within protein beta-strands 蛋白质-链内左旋扭曲的几何准则

生物物理化学(英文) Pub Date : 2014-01-15 DOI: 10.4236/JBPC.2014.51002

B. Caudron, J. Jestin

引用次数: 1

Antioxidants as effective remedies at hepatotoxic action of carbon tetrachloride 抗氧化剂对四氯化碳肝毒性作用的有效补救

生物物理化学(英文) Pub Date : 2014-01-15 DOI: 10.4236/JBPC.2014.51001

K. Hovnanyan, V. Mamikonyan, A. Margaryan, K. Sargsyan, M. Hovnanyan, M. Karagyozyan, K. Karageuzyan

引用次数: 7

Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method 水滴法观察水表面双棕榈酰磷脂酰胆碱(DPPC)单分子膜的形态

生物物理化学(英文) Pub Date : 2013-11-21 DOI: 10.4236/JBPC.2013.44016

D. Yoshida, T. Yokoyama, T. Shimoaki, T. Tomita, T. Yoshida, Yasushi Yamamoto, K. Taga, Ayumi Sumino, Takehisa Dewa, M. Nango, Masato Yamamoto, Zameer Sheravani

引用次数: 11