生物物理化学(英文)最新文献_第5页

Maintained Exposure to Spring Water but Not Double Distilled Water in Darkness and Thixotropic Conditions to Weak (~1 µT) Temporally Patterned Magnetic Fields Shift Photon Spectroscopic Wavelengths: Effects of Different Shielding Materials 在黑暗和触变条件下保持暴露于泉水而不是双重蒸馏水中，以弱(~1µT)时间图案磁场位移光子光谱波长:不同屏蔽材料的影响

生物物理化学(英文) Pub Date : 2015-01-08 DOI: 10.4236/JBPC.2015.61002

N. Murugan, L. Karbowski, R. Lafrenie, M. Persinger

{"title":"Maintained Exposure to Spring Water but Not Double Distilled Water in Darkness and Thixotropic Conditions to Weak (~1 µT) Temporally Patterned Magnetic Fields Shift Photon Spectroscopic Wavelengths: Effects of Different Shielding Materials","authors":"N. Murugan, L. Karbowski, R. Lafrenie, M. Persinger","doi":"10.4236/JBPC.2015.61002","DOIUrl":"https://doi.org/10.4236/JBPC.2015.61002","url":null,"abstract":"Spring water but not double-distilled water was exposed, in darkness, to a temporally patterned weak magnetic field that has been shown to affect planarian behavior and slow the rate of cancer cell proliferation. Exposure to the magnetic field caused a reliable shift in the peak (longer) wave-length of ~10 nm for fluorescence emissions and a ~20% increase (~100 counts) in fluorescence intensity. Spectral analyses verified a shift of 5 and 10 nm, equivalent to ~1.5 × 10-20 J “periodicity” across the measured wavelengths, which could reflect a change in the an intrinsic energy as predicted by Del Giudice and Preparata and could correspond to two lengths of O-H bonds. Wrapping the water sample containers during exposure with copper foil, aluminum foil, or plastic altered these fluorescent profiles. The most conspicuous effect was the elimination of a ~280 nm peak in the UV-VIS emission spectra only for samples wrapped with copper foil but not aluminum or plastic. These results suggest that weak magnetic fields produce alterations in the water-ionic complexes sufficient to be reliably measured by spectrophotometry. Because the effect was most pronounced when the spring water was exposed in darkness and was not disturbed the role of thixotropic phenomena and Del Giudice entrapment of magnetic fields within coherent domains of Pollack virtual exclusion zones (EZ) may have set the conditions for subsequent release of the energy as photons.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70903525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Retracted HIV Study Provides New Information about the Status of the in Vitro Inhibition of DNA Replication by Backbone Methylation 撤回的HIV研究提供了关于主链甲基化在体外抑制DNA复制状态的新信息

生物物理化学(英文) Pub Date : 2015-01-08 DOI: 10.4236/JBPC.2015.61003

H. Buck

引用次数: 3

Dynamic Changes in Lipid Peroxidation and Antioxidant Level in Rat’s Tissues with Macrovipera lebetina obtusa and Montivipera raddei Venom Intoxication 大鼠粗白蝮蛇和黑蝮蛇毒液中毒后组织脂质过氧化和抗氧化水平的动态变化

生物物理化学(英文) Pub Date : 2014-11-14 DOI: 10.4236/JBPC.2014.54017

N. Zaqaryan, N. Ghazaryan, N. Ayvazyan

{"title":"Dynamic Changes in Lipid Peroxidation and Antioxidant Level in Rat’s Tissues with Macrovipera lebetina obtusa and Montivipera raddei Venom Intoxication","authors":"N. Zaqaryan, N. Ghazaryan, N. Ayvazyan","doi":"10.4236/JBPC.2014.54017","DOIUrl":"https://doi.org/10.4236/JBPC.2014.54017","url":null,"abstract":"We investigated the balance of free radicals in different tissues (liver, heart, brain and muscle) of rats in course of in vivo and in vitro processing by Macrovipera lebetina obtusa (MLO) and Montivipera raddei (MR) snake venoms. Chemiluminescence (ChL) levels were examined in tissue assays after incubation (at 37 °C for a period of 10 min) with venom for in vitro experiments and in tissue assays isolated of 10 min after venom injection for in vivo experiments. The TBA-test was also performed to confirm the free radical expression. The activities of antioxidant enzymes (such as superoxide dismutase and glutathione peroxidase) in isolated tissues were detected by spectro-photometry. During the in vitro processing chemiluminescence levels of tissue homogenates significantly decreased, while in course of in vivo intoxication the level of ChL was elevated in brain and liver; lipid peroxidation also increased in brain tissue, but there was no significant balance change in other tissues; the activity of superoxide dismutase mainly correlated with changes of free radical balance during intoxication. On the contrary, the activity of glutathione peroxidase showed the reverse tendencies to change. We suggest that free radicals and their oxidative stresses may play a role in the early stage of intoxication causing the so-named “spreading-effect”, which is very characteristic for the venom of vipers.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70903336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Volume Change of the Random Coil to Folded Conformational Transition of Thermomyces lanuginosus Xylanase at 24°C and pH = 7.0 via Application of the Clausius-Clapeyron Equation 应用Clausius-Clapeyron方程研究24°C和pH = 7.0条件下褐热藓木聚糖酶随机螺旋形到折叠构象转变的体积变化

生物物理化学(英文) Pub Date : 2014-11-14 DOI: 10.4236/JBPC.2014.54015

H. Wilks, T. Arrington, B. M. Britt

引用次数: 0

Thermodynamics of the Second Dissociation Constants (pK2) of Piperazine-N,N′-bis-2-hydroxypropanesulfonic Acid (POPSO Sesquisodium Salt) and Associated Thermodynamic Functions from (278.15 to 328.15) K (278.15 ~ 328.15) K范围内哌嗪-N,N ' -双-2-羟基丙磺酸(POPSO倍半钠盐)第二解离常数(pK2)的热力学及相关热力学函数

生物物理化学(英文) Pub Date : 2014-11-14 DOI: 10.4236/JBPC.2014.54016

R. Roy, L. N. Roy, Katherine E. Hundley, Taylor R. Wehmeyer, Lucas S. Tebbe

{"title":"Thermodynamics of the Second Dissociation Constants (pK2) of Piperazine-N,N′-bis-2-hydroxypropanesulfonic Acid (POPSO Sesquisodium Salt) and Associated Thermodynamic Functions from (278.15 to 328.15) K","authors":"R. Roy, L. N. Roy, Katherine E. Hundley, Taylor R. Wehmeyer, Lucas S. Tebbe","doi":"10.4236/JBPC.2014.54016","DOIUrl":"https://doi.org/10.4236/JBPC.2014.54016","url":null,"abstract":"The second acidic dissociation constants of protonated piperazine-N,N′-bis-2-hydroxypropane-sulfonic acid (POPSO sesquisodium salt) have been determined at 12 different temperatures from (278.15 to 328.15) K including 310.15 K. Electromotive-force measurement technique was used employing hydrogen-silver chloride cells without liquid junction. The results of pK2 are given by the equation: pK2 = -1041.77/T + 51.0459 - 6.97646lnT. The uncertainty of the fit is ±0.0008. At 289.15 K, pK2 = 7.8029; whereas, at 310.15 K (body temperature), pK2 = 7.6862. Thus, the buffer solutions of POPSO and its sodium salt are useful for pH control in the physiological pH region of (7.0 to 8.5). The changes of Gibbs free energy (G°), enthalpy (H°), entropy (S°) and heat capacity Cp° were computed from the temperature derivative of the pK2 for the dissociation of the zwitterionic acid POPSO±-3 = POPSO-4 + H+ in the standard state. At 298.15 K, these results are compared with those of similar components, which are the derivatives of the parent compounds TAURINE, PIPERAZINE and MORPHOLINE.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70903012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consequences of Non-Uniformity in the Stoichiometry of Component Fractions within One and Two Loops Models of α-Helical Peptides α-螺旋肽一环和二环模型中组分组分化学计量不均匀的后果

生物物理化学(英文) Pub Date : 2014-11-14 DOI: 10.4236/JBPC.2014.54014

W. Schmidt, C. Hapeman, J. Wachira, Clayton Thomas

{"title":"Consequences of Non-Uniformity in the Stoichiometry of Component Fractions within One and Two Loops Models of α-Helical Peptides","authors":"W. Schmidt, C. Hapeman, J. Wachira, Clayton Thomas","doi":"10.4236/JBPC.2014.54014","DOIUrl":"https://doi.org/10.4236/JBPC.2014.54014","url":null,"abstract":"A 3-D electrostatic density map generated using the Wavefront Topology System and Finite Element Method clearly demonstrates the non-uniformity and periodicity present in even a single loop of an α-helix. The four dihedral angles (N-C*-C-N, C*-C-N-C*, and C-N-C*-C) fully define a helical shape independent of its length: the three dihedral angles, φ = -33.5°, ω = 177.3°, and Ψ = -69.4°, generate the precise (and identical) redundancy in a one loop (or longer) α-helical shape (pitch = 1.59 /residue; r = 2.25 ). Nevertheless the pattern of dihedral angles within an 11 and a 22-peptide backbone atom sequence cannot be distributed evenly because the stoichiometry in fraction of four atoms never divides evenly into 11 or 22 backbone atoms. Thus, three sequential sets of 11 backbone atoms in an α-helix will have a discretely different chemical formula and correspondingly different combinations of molecular forces depending upon the assigned starting atom in an 11-step sequence. We propose that the unit cell of one loop of an α-helix occurs in the peptide backbone sequence C-(N-C*-C)3-N which contains an odd number of C* plus even number of amide groups. A two-loop pattern (C*-C-N)7-C* contains an even number of C* atoms plus an odd number of amide groups. Dividing the two-loop pattern into two equal lengths, one fraction will have an extra half amide (N-H) and the other fraction will have an extra half amide C=O, i.e., the stoichiometry of each half will be different. Also, since the length of N-C*-C-N, C*-C-N-C*, and C-N-C*-C are unequal, the summation of the number of each in any fraction of n loops of an α-helix in sequence will always have unequal length, depending upon the starting atom (N, C*, or C).","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70903226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Thermodynamics of the Second Stage Dissociation Step (pK 2 ) of Buffer Monosodium 1,4-Piperazinediethanesulfonate from (278.15 to 328.15) K 缓冲液1,4-哌嗪二烷基磺酸钠从(278.15)K到(328.15)K的第二阶段解离(pK 2)热力学

生物物理化学(英文) Pub Date : 2014-06-10 DOI: 10.4236/JBPC.2014.53010

R. Roy, L. N. Roy, J. Dinga, Matthew R. Medcalf, Katherine E. Hundley, E. Hines, Ryan R. Parmar, Jamie Veliz, Clark B. Summers, Lucas S. Tebbe

{"title":"Thermodynamics of the Second Stage Dissociation Step (pK 2 ) of Buffer Monosodium 1,4-Piperazinediethanesulfonate from (278.15 to 328.15) K","authors":"R. Roy, L. N. Roy, J. Dinga, Matthew R. Medcalf, Katherine E. Hundley, E. Hines, Ryan R. Parmar, Jamie Veliz, Clark B. Summers, Lucas S. Tebbe","doi":"10.4236/JBPC.2014.53010","DOIUrl":"https://doi.org/10.4236/JBPC.2014.53010","url":null,"abstract":"Values of the second thermodynamic dissociation constant pK2 of the protonated form of monosodium 1,4-piperazinediethanesulfonate (PIPES) have been determined at twelve different temperatures in the temperature range from (278.15 to 328.15) K including the body temperature 310.15 K by measurement of the electromotive-force for cells without liquid junction of the type: Pt (s), H2 (g), 101.325 kPa|Na-PIPES (m1) + Na 2-PIPES (m2) + NaCl (m3)|AgCl (s), Ag (s), where m1, m2 and m3 indicate the molalities of the corresponding species at 1 atm = 101.325 kPa in SI units. The pK2 values for the dissociation of Na-PIPES are represented by the equation: pK2 = -1303.76/T + 48.369 - 6.46889 lnT with an uncertainty of ± 0.001. The values of pK2 for Na-PIPES were found to be 7.1399 ± 0.0004 at 298.15 K and 7.0512 ± 0.0004 at 310.15 K, respectively, and indicate that this buffer would be useful as pH standard in the range of physiological application. Standard thermodynamic quantities for the acidic dissociation process of Na-PIPES have been derived from the temperature coefficients of the pK2. These values are compared with those of structurally related N-substituted PIPERAZINE and TAURINE at 298.15 K.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70902942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Acid Dissociation Constants and Related Thermodynamic Functions of Protonated 2,2-Bis(Hydroxymethyl)-2,2’,2”- Nitrilotriethanol (BIS-TRIS) from (278.15 to 328.15) K (278.15 ~ 328.15) K质子化2,2-二(羟甲基)-2,2′，2”-硝基三乙醇(BIS-TRIS)的酸解离常数及相关热力学函数

生物物理化学(英文) Pub Date : 2014-06-10 DOI: 10.4236/JBPC.2014.53013

R. Roy, L. N. Roy, Katherine E. Hundley, J. Dinga, Mathew R. Medcalf, Lucas S. Tebbe, Ryan R. Parmar, J. A. Veliz

{"title":"Acid Dissociation Constants and Related Thermodynamic Functions of Protonated 2,2-Bis(Hydroxymethyl)-2,2’,2”- Nitrilotriethanol (BIS-TRIS) from (278.15 to 328.15) K","authors":"R. Roy, L. N. Roy, Katherine E. Hundley, J. Dinga, Mathew R. Medcalf, Lucas S. Tebbe, Ryan R. Parmar, J. A. Veliz","doi":"10.4236/JBPC.2014.53013","DOIUrl":"https://doi.org/10.4236/JBPC.2014.53013","url":null,"abstract":"Thermodynamic dissociation constants pKa of 2,2-bis(hydroxymethyl)-2,2’,2”-nitrilotriethanol have been determined at 12 temperatures from (278.15 to 328.15) K including the body temperature 310.15 K by the electromotive-force measurements (emf) of hydrogen-silver chloride cells without liquid junction of the type: Pt(s), H2(g), 101.325 kPa|BIS-TRIS (m) + BIS-TRIS·HCl (m)| AgCl(s), Ag(s), where m denotes molality. The pKa values for the dissociation process of BIS-TRIS·H++ H2O = H3O+ + BIS-TRIS given as a function of T in Kelvin (K) by the equation pKa = 921.66 (K/T) + 14.0007-1.86197 ln(T/K). At 298.15 and 310.15 K, the values of pKa for BIS-TRIS were found to be 6.4828 ± 0.0005 and 6.2906 ± 0.0006 respectively. Thus buffer solutions composed of BIS-TRIS and its hydrochloride would be useful as secondary pH buffer standards and for control of acidity in the pH range 6 to 8. At 298.15 K the thermodynamic functions G°, H°, S° and Cp° for the dissociation process of BIS-TRIS·H+ are G°=37,005 J·mol-1, H° = 28,273 J·mol-1, S°= 29.3 J·K-1·mol-1 and Cp° = 36 J·K-1·mol-1. These results are compared with the dissociation of protonated bases structurally related to BIS-TRIS·H+.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70903321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

DNA Variation of Capoeta damascina (Valenciennes, 1842) in Three Rivers in Northern Israel 巴伦西亚，1842年在以色列北部三河地区发现的大马士革卡波塔(Capoeta damascina)的DNA变异

生物物理化学(英文) Pub Date : 2014-06-10 DOI: 10.4236/JBPC.2014.53012

G. Degani

{"title":"DNA Variation of Capoeta damascina (Valenciennes, 1842) in Three Rivers in Northern Israel","authors":"G. Degani","doi":"10.4236/JBPC.2014.53012","DOIUrl":"https://doi.org/10.4236/JBPC.2014.53012","url":null,"abstract":"The present study is in agreement with the hypothesis that the variation of ecological conditions in three rivers in northern Israel—the Dan, Hasbani and Hermon Rivers—affects the genetic variations of the species Capoeta damascina. Using mitochondrial DNA (mtDNA), cytochrome b gene (Cytb), 16S and nuclear DNA (nDNA), and Random Amplified Polymorphic DNA (RAPD), four different clusters were found in the Cytb of the Hasbani and Hermon Rivers and only two in the Dan River. Moreover, the clusters in the Hasbani River differed from those found in the Hermon River. A similar result was found when an analysis was made of a different sequence from five different haplotype frequencies using the MegAlign program, the lowest being in the Dan River (only two haplotypes) and the highest in the Hasbani River (four haplotypes). The analysis of molecular variance of Cytb and 16S (AMOVA) for individuals of C. damascina from eight populations in northern Israel showed significant differences between the rivers and the populations. The analysis by mitochondrial 16S of haplotype frequencies of C. damascina populations in the rivers in northern Israel was very low compared to Ctb. Sixteen different haplotypes were found in the different rivers: eight in the Hasbani River, seven in the Dan River and only five in the Hermon River.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70903117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Intraflagellar Protein 88 Interactome Analysis: A Bioinformatics Approach Highlights Its Role in Testis and Sperm Function 鞭毛内蛋白88相互作用组分析:生物信息学方法强调其在睾丸和精子功能中的作用

生物物理化学(英文) Pub Date : 2014-06-10 DOI: 10.4236/JBPC.2014.53011

M. J. Freitas, M. Fardilha

引用次数: 0