生物物理化学(英文)最新文献_第10页

Vibrational spectra of distorted structure Macro & Nano molecules: An algebraic approach 扭曲结构宏观与纳米分子的振动谱:一种代数方法

生物物理化学(英文) Pub Date : 2012-08-28 DOI: 10.4236/JBPC.2012.33031

S. Karumuri, J. Vijayasekhar, V. Rao, G. Srinivas, A. Hanumaiah

引用次数: 1

The low frequency electromagnetic field on the rat EEG 低频电磁场对大鼠脑电图的影响

生物物理化学(英文) Pub Date : 2012-08-28 DOI: 10.4236/JBPC.2012.33026

S. Sallam

引用次数: 1

Homology modeling and structural analysis of human γ-glutamylcysteine ligase catalytic subunit for antitumor drug development 人γ-谷氨酰半胱氨酸连接酶催化亚基抗肿瘤药物开发的同源性建模及结构分析

生物物理化学(英文) Pub Date : 2012-08-28 DOI: 10.4236/JBPC.2012.33028

H. Yamaguchi, T. Akitaya, Y. Kidachi, K. Kamiie, H. Umetsu

{"title":"Homology modeling and structural analysis of human γ-glutamylcysteine ligase catalytic subunit for antitumor drug development","authors":"H. Yamaguchi, T. Akitaya, Y. Kidachi, K. Kamiie, H. Umetsu","doi":"10.4236/JBPC.2012.33028","DOIUrl":"https://doi.org/10.4236/JBPC.2012.33028","url":null,"abstract":"Homology modeling and structural analysis of human glutamate cysteine ligase catalytic subunit (hGCLC) were performed with a software package the Molecular Operating Environment. A yeast GCLC (yGCLC; PDB code: 3LVV) was selected as a template for the 3D structure modeling of hGCLC. The modeled hGCLC showed significant 3D similarities at the ligand biding site (LBS) to the yGCLC structure. The contact energy profiles of the hGCLC model were in good agreement with those of the yGCLC structure. Ramachandran plots revealed that only 1.4% of the amino acid residues were in the disfavored region for hGCLC. The molecular electrostatic potential (MEP) map of the hGCLC model exhibited that the model was slightly different from the yGCLC model electrostatically at the LBS. Further, docking simulations revealed the similarity of the ligand-receptor bound location between the hGCLC and yGCLC models. The different binding orientations between the glutathione (GSH)-hGCLC and GSH-yGCLC complexes reflected the different MEP maps at the LBSs between the hGCLC and yGCLC models. These results indicate that the hGCLC model was successfully modeled and analyzed. To the best of our knowledge, this is the first report of a hGCLC model with detailed analyses, and our data verify that the model can be utilized for application to target hGCLC for the development of anticancer drugs.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":"3 1","pages":"238-248"},"PeriodicalIF":0.0,"publicationDate":"2012-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70901591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular basis of scavenging effect of zonisamide on hydroxyl radical in vitro 唑尼沙胺体外清除羟基自由基的分子基础

生物物理化学(英文) Pub Date : 2012-08-28 DOI: 10.4236/JBPC.2012.33030

Ken-ichi Tanaka, T. Nanba, Tomoyuki Furubayashi, Y. Noda, L. Willmore, A. Mori

引用次数: 1

Insilico studies of 2-methylheptyl isonicotinate produced by Streptomyces sps. 201 against dihydrodipicolinate synthase enzyme of Mycobacterium tuberculosis 链霉菌合成异烟酸2-甲基庚酯的硅化研究。201抗结核分枝杆菌二氢二吡啶酸合酶

生物物理化学(英文) Pub Date : 2012-08-28 DOI: 10.4236/JBPC.2012.33027

S. P. Singh, R. L. Bezbaruah, T. Bora

引用次数: 5

Build mutant and build homology protein structure predictions for indonesian avian influenza neuraminidase 构建印尼禽流感神经氨酸酶突变体及同源蛋白结构预测

生物物理化学(英文) Pub Date : 2012-05-29 DOI: 10.4236/JBPC.2012.32020

S. Herlambang, R. Saleh

{"title":"Build mutant and build homology protein structure predictions for indonesian avian influenza neuraminidase","authors":"S. Herlambang, R. Saleh","doi":"10.4236/JBPC.2012.32020","DOIUrl":"https://doi.org/10.4236/JBPC.2012.32020","url":null,"abstract":"Protein structure modeling using a homologous template is one of many routines that accompany the molecular dynamics simulation for biological material. There are currently two protocols of protein modeling available in Accelrys Discovery Studio 2.1, Build Mutants and Build Homology Modeling protocols. Both are template-based modeling, but with a different process. In this study, two different templates, 3CKZ and 274Y, have been used to see how much the differences will be made by those two protocols if the templates has significant percentage of identity. Evaluation of structure models has been performed using DOPE score, 3D-profile, and PROCHECK. The results indicated that Build Mutants Protocols produces more stable structures but has a low reliability values and low quality of stereochemistry when using a template that has a lower percentage of identity. The results also yield more stable, reliable, and higher percentage of residues in most favoured and additionally allowed region for the usage of Build Homology Modeling Protocol on both templates. These observations suggest that Build Homology Modeling protocol is recommended for protein modeling.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":"3 1","pages":"183-190"},"PeriodicalIF":0.0,"publicationDate":"2012-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70901227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

N-acetyl-L-cysteine amide protects retinal pigment epithelium against methamphetamine-induced oxidative stress n -乙酰- l-半胱氨酸酰胺保护视网膜色素上皮免受甲基苯丙胺诱导的氧化应激

生物物理化学(英文) Pub Date : 2012-05-29 DOI: 10.4236/JBPC.2012.32012

J. W. Carey, Shakila Tobwala, Xinsheng Zhang, Atrayee Banerjee, N. Ercal, E. Pınarcı, H. Karacal

{"title":"N-acetyl-L-cysteine amide protects retinal pigment epithelium against methamphetamine-induced oxidative stress","authors":"J. W. Carey, Shakila Tobwala, Xinsheng Zhang, Atrayee Banerjee, N. Ercal, E. Pınarcı, H. Karacal","doi":"10.4236/JBPC.2012.32012","DOIUrl":"https://doi.org/10.4236/JBPC.2012.32012","url":null,"abstract":"Methamphetamine (METH), a highly addictive drug used worldwide, induces oxidative stress in various animal organs. Recent animal studies indicate that methamphetamine also induces oxidative stress in the retina, which is an embryonic extension of the forebrain. The aim of this study, therefore, was to evaluate the protecttive effects of N-acetylcysteine amide (NACA) against oxidative stress induced by METH in retinal pigment epithelium (RPE) cells. Our studies showed that NACA protected against METH-induced oxidative stress in retinal pigment epithelial cells. Although METH significantly decreased glutathione (GSH) levels and increased reactive oxygen species (ROS) and malondialdehyde (MDA) levels, these returned to control levels with NACA treatment. Overall observations indicated that NACA protected RPE cells against oxidative cell damage and death by inhibiting lipid peroxidation, scavenging ROS, increasing levels of intracellular GSH, and maintaining the antioxidant enzyme activity and the integrity of the bloodretinal barrier (BRB). The effectiveness of NACA should be further evaluated to determine its potential for the treatment of numerous retinal diseases caused by oxidative stress.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":"3 1","pages":"101-110"},"PeriodicalIF":0.0,"publicationDate":"2012-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70900943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions 生理条件下大鼠小肠葡萄糖吸收的动力学和机制

生物物理化学(英文) Pub Date : 2012-05-29 DOI: 10.4236/JBPC.2012.32021

A. Gruzdkov, L. V. Gromova, N. Grefner, Y. Komissarchik

{"title":"Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions","authors":"A. Gruzdkov, L. V. Gromova, N. Grefner, Y. Komissarchik","doi":"10.4236/JBPC.2012.32021","DOIUrl":"https://doi.org/10.4236/JBPC.2012.32021","url":null,"abstract":"Previous studies have shown two components of glucose absorption in the small intestine: a secondary active transport through SGLT1, and unsaturated component, recently attributed mainly to the facilitated diffusion through GLUT2, but the relationship between these two components under physiological conditions remains controversial. In chronic experiments on nonanesthetized rats we investigated for the first time the kinetics of maltose hydrolysis and glucose absorption in the isolated loop of the small intestine in a wide range of maltose and glucose concentrations (25 ÷ 200 mmol/l glucose). The processes were simulated on mathematical models which took into account the current views about mechanisms of hydrolysis and transport of nutrients and geometric characteristics of the intestinal surface. The results of chronic experiments and mathematical simulation have shown that under the close to physiological conditions the glucose transport mediated by SGLT1 is the main mechanism of its absorption in comparison with the unsaturated component. This was demonstrated not only at low, but also at high substrate concentrations. We conclude that correct evaluation of the relative contribution of different mechanisms in glucose transport through the intestinal epithelium requires taking into account the geometric specificities of its surface.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":"3 1","pages":"191-200"},"PeriodicalIF":0.0,"publicationDate":"2012-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70901249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cancer chemoprevention through the induction of apoptosis by natural compounds 通过天然化合物诱导细胞凋亡的癌症化学预防

生物物理化学(英文) Pub Date : 2012-05-29 DOI: 10.4236/JBPC.2012.32018

T. Kuno, Testuya Tsukamoto, A. Hara, Takuji Tanaka

{"title":"Cancer chemoprevention through the induction of apoptosis by natural compounds","authors":"T. Kuno, Testuya Tsukamoto, A. Hara, Takuji Tanaka","doi":"10.4236/JBPC.2012.32018","DOIUrl":"https://doi.org/10.4236/JBPC.2012.32018","url":null,"abstract":"As cell and tissue homeostasis are mediated by the balance between proliferation and apoptosis, controlling this balance is important for cancer chemoprevention. Cancer chemoprevention can be achieved by the use of natural, synthetic or biologic compounds that reverse, suppress or prevent the development of epithelial malignancies. Natural compounds including flavonoids are able to reduce oxidative stress, which is the most likely mechanism mediating the protective effects against cancer development. In addition, in vitro and in vivo studies have suggested that flavonoids, such as (-)-epigallocatechin-3-gallete (EGCG), quercetin, and curcumin, act by induction of apoptosis. Several natural compounds inhibit cell proliferation and angiogenesis. Certain natural products have been shown to inhibit the activation of nuclear factor kappa B (NF-κB) and Akt signaling pathways, both of which are known to maintain a homeostatic balance between cell survival and apoptosis. Understanding the mechanism of these natural products will contribute to the development of more specific preventive strategies against cancer development. Here we focus on the ability of natural cancer chemopreventive agents to induce apoptosis, and attempt to provide evidence for the preventive and therapeutic effects of natural compounds, EGCG, quercetin, and curcumin, in a succinct manner highlightingκand Akt signaling pathways in vivo.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":"3 1","pages":"156-173"},"PeriodicalIF":0.0,"publicationDate":"2012-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4236/JBPC.2012.32018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70900981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 123

Role of endothelium on the abnormal Angiotensin-mediated vascular functions in epileptic rats 内皮在癫痫大鼠血管紧张素介导的异常血管功能中的作用

生物物理化学(英文) Pub Date : 2012-05-29 DOI: 10.4236/JBPC.2012.32019

Carolina Baraldi Araujo Restini, Rosana I. Reis, C. Costa-Neto, N. Garcia-Cairasco, José A. Cortes‐de‐Oliveira, L. Bendhack

{"title":"Role of endothelium on the abnormal Angiotensin-mediated vascular functions in epileptic rats","authors":"Carolina Baraldi Araujo Restini, Rosana I. Reis, C. Costa-Neto, N. Garcia-Cairasco, José A. Cortes‐de‐Oliveira, L. Bendhack","doi":"10.4236/JBPC.2012.32019","DOIUrl":"https://doi.org/10.4236/JBPC.2012.32019","url":null,"abstract":"Epidemiological studies have found that the risk for cardiovascular disease is increased in patients with epilepsy. The Renin Angiontensin System (RAS), an important player in vascular tone control, is also involved in many neurological disorders, including seizures and epilepsy. Although it has been reported that Angiotensin II (Ang II) release and Angiotensin receptors expression are altered in many cerebral areas in patients/animal models with neurological disorders, there are no data on the vascular function. We evaluated Ang I and Ang II-mediated vascular responses and to correlate their contractile responses to the pres- ence of endothelium and the protein levels of components of the RAS (AT1, AT2, Mas and ACE) in aorta isolated from genetically epileptic rats (WAR strain). The major finding was that the vascular contractile response induced by Ang I and Ang II is endothelium-dependent. Ang II induced contractions in aortas from Wistar rats either with intact endothelium (E+) (1.16 ± 0.04 g, n = 6) and endothelium-denuded (E-) (1.24 ± 0.04 g, n = 6). Maximum contractile response (ME) induced by Ang I was lower in Wistar E+ (0.45 ± 0.03 g, n = 6) compared with Wistar E- (1.13 ± 0.08 g, n = 6). Ang I and Ang II failed to induce contraction in WAR E+, whereas the ME induced by Ang I in WAR E- was lower (0.52 ± 0.04 g, n = 11) than in the Wistar. ME induced by Ang II in aortas from WAR was also lower (0.40 ± 0.03 g, n = 11) compared with Wistar. AT1 receptor expression in both E+ WAR and Wistar was lower than in both E- WAR and Wistar. AT2 and Mas receptor expression was higher in Wistar E- and E+ as compared to WAR E- and E+. ACE expression was higher in both E+ WAR and Wistar, but it was lower in both E- WAR and Wistar. Endothelium impairs the contractile response induced by Angiotensin in WAR, suggesting that endothelial relaxing factors play important role on the aorta contraction.","PeriodicalId":62927,"journal":{"name":"生物物理化学(英文)","volume":"3 1","pages":"174-182"},"PeriodicalIF":0.0,"publicationDate":"2012-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70901157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2