Journal of Analytical Chemistry最新文献

{"title":"Sorption–Fluorimetric Determination of Enrofloxacin Using Fibers Formed by Electrospinning","authors":"A. V. Syardina,&nbsp;E. V. Yurova,&nbsp;T. D. Smirnova,&nbsp;T. Yu. Rusanova","doi":"10.1134/S1061934826700553","DOIUrl":"10.1134/S1061934826700553","url":null,"abstract":"<p>The work is devoted to the development of a method for the sorption–fluorometric determination of enrofloxacin in aqueous solutions using sensitized fluorescence on the surface of a nanofiber formed by electrospinning. Enrofloxacin is a broad-spectrum antibacterial agent; as an inhibitor of bacterial DNA gyrase, it is widely used in livestock, poultry, and fisheries, and as a growth promoter and in food additives. However, the wide and often unjustified use of antibacterial substances is a reason for exceeding the permissible values of their residual quantities in the food raw materials and food products and in natural and waste waters, which necessitates monitoring of their contents. The effect of the nature of polymer fibers, the concentration of terbium ions, the acidity of the medium, and the nature of the surfactant on the recovery of enrofloxacin and the fluorescence intensity of the analytical system was studied. Polyacrylonitrile fiber formed from a 13% solution in dimethylformamide by electrospinning was selected as a sorbent. It was shown that, in the presence of terbium ions, the recovery of fluoroquinolone increased fivefold and reached 91%. The increase in this indicator is associated with the enhancement of electrostatic and hydrophobic interactions of the chelate with the sorbent, as well as with the possible implementation of the coordination binding of terbium ions with the nitrile group of polyacrylonitrile. The addition of micelles of cationic, nonionic, and anionic surfactants has virtually no effect on the recovery of enrofloxacin. The optimal sorption time was 90 min. It was shown that multilayer adsorption structures could form on the fiber surface as the enrofloxacin concentration was increased. The proposed sorption–fluorometric method for the determination of enrofloxacin is characterized by a low limit of detection of 1 × 10^–7 M and a fairly wide analytical range of 4 × 10^–7−5 × 10^–4 M. The method was tested on natural waters, and its accuracy was controlled by the added–found method.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1146 - 1151"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Authentication of Citronella Oil from Turpentine Oil Using Fourier-Transform Infrared Spectroscopy Followed by Principal Component Analysis and Partial Least Squares Regression","authors":"Afnan Syifa’ Muhammad,&nbsp;Agustina Ari Murti Budi Hastuti","doi":"10.1134/S1061934825603196","DOIUrl":"10.1134/S1061934825603196","url":null,"abstract":"<p>The adulteration of citronella (<i>Cymbopogon winterianus</i>) oil with a cheaper alternative, such as low-grade turpentine oil, is a recurring issue in the essential oil industry, posing risks to product quality and consumer safety. A rapid and non-destructive authentication method is therefore essential for quality control of this product. This study utilized Fourier-transform infrared spectroscopy followed by principal component analysis (<b>PCA</b>) and partial least squares (<b>PLS</b>) regression for qualitative and quantitative authentication of citronella oil mixed with turpentine oil, respectively. Samples were prepared with various adulteration concentrations of citronella oil in turpentine oil (0 to 100%). Additionally, several commercial products were analyzed. The PCA results showed that the best model was obtained using normal spectra without any pretreatment in the wavenumber range of 1200–900 cm^–1. This model demonstrated high discrimination ability with eigenvalues of 95.65% for PC1 and 3.84% for PC2. For PLS analysis, the best model was developed using first-derivative pretreated spectra in the wavenumber range of 3600–1600 cm^–1. The PLS calibration model produced the equation <i>y</i> = 0.99998<i>x</i> – 0.00001, with an <i>R</i>² value of 1.0000 and a root mean square error of calibration of 0.00038, indicating excellent linearity. The PLS validation model resulted in the equation <i>y</i> = 1.0094<i>x</i> – 0.0007, with an <i>R</i>² value of 0.9998 and a root mean square error of prediction of 0.0085, confirming the accuracy and precision of the model in detecting citronella oil adulterated with turpentine oil.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1244 - 1249"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Phospholipids in Infant Formula Using Infrared Spectroscopy and Chemometric Data Analysis","authors":"K. T. Aitalieva,&nbsp;N. A. Burmistrova,&nbsp;Yu. B. Monakhova","doi":"10.1134/S106193482670053X","DOIUrl":"10.1134/S106193482670053X","url":null,"abstract":"<p>Infant formula is an alternative to breast milk for newborns when breastfeeding is not possible for any reason. This study examines vibrational spectroscopic methods, namely near-infrared spectroscopy (NIR) and attenuated total reflectance infrared spectroscopy (ATR-IR) in the mid-infrared region, for the determination of phospholipids in infant nutrition products. Chemometric methods were applied for processing the spectroscopic data, including principal component analysis (PCA) and partial least squares regression (PLS). PCA demonstrated clustering of samples into groups according to their phospholipid content. Using PLS models based on ATR-IR and NIR spectra, it is possible to determine major classes of phospholipids with sufficient accuracy, including phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin, as well as total phosphorus content (coefficients of determination <i>R</i>² greater than 0.94 and 0.96 for ATR-IR and NIR data, respectively; mean squared prediction error less than 0.14% for both methods). Spectroscopic methods combined with chemometric approaches represent an effective tool for quality control of infant nutrition products.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1126 - 1132"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-Friendly Spectrophotometric and Paper-Based Microfluidic Techniques for Creatinine Determination in Serum","authors":"Elaf A. Abduljallel,&nbsp; Mohammad K. Hammood","doi":"10.1134/S1061934826700619","DOIUrl":"10.1134/S1061934826700619","url":null,"abstract":"<p>Creatinine is a key marker for renal function, and accurate measurement of creatinine in serum is important for clinical diagnostics. In the present work, a new colorimetric method using the creatinine-chlorophenol red (<b>CPR</b>) reaction was introduced and conducted with ultraviolet-visible and paper-based microfluidic (<b>µPAD</b>) measurement. Optimal conditions were established for each method, whereas analytical performance in terms of linearity, sensitivity, precision, recovery, and comparison with the reference analyzer (Cobas c311) was evaluated. A spectrophotometric method based on the formation of a pink-violet ion-pair complex and its bathochromic shift with an absorption maximum at 576 nm was developed, linear over 2–130 µg/mL, the limit of detection (<b>LOD</b>) was 1.2 µg/mL, and the limit of quantification (<b>LOQ</b>) was 2.25 µg/mL. Finally, the µPAD-based assay was optimized using a higher CPR concentration (100 µg/mL) and had a linear range of 10–160 µg/mL (<i>R</i>² = 0.9972), and LOD and LOQ were 7.0 and 11.57 µg/mL, respectively. Recovery values between 97.1 and 104.3%, corresponding closely to values obtained using a Cobas c311 analyzer, were achieved. The two procedures followed the tenets of Green Analytical Chemistry. These results underscore the robustness, sensitivity, and long-term stability of these approaches, thereby indicating their potential suitability for both laboratory-based centralized detection and point-of-care decentralized diagnostics.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1204 - 1216"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nondestructive Testing of Food Products and Pharmaceuticals by Smartphone-Based Digital Colorimetry","authors":"O. E. Emelyanov,&nbsp;V. G. Amelin","doi":"10.1134/S1061934826700498","DOIUrl":"10.1134/S1061934826700498","url":null,"abstract":"<p>This paper presents the results of original research on nondestructive quality control of food products and solid dosage forms of pharmaceuticals using digital colorimetry with a smartphone and chemometric processing of the resulting spectral data.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1087 - 1100"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision and Sensitivity of Photothermal Thermal-Lens Measurements in Aqueous Polyethylene Glycol Solutions","authors":"V. R. Khabibullin,&nbsp;E. S. Ryndina,&nbsp;I. V. Mikheev,&nbsp;S. V. Krasheninina,&nbsp;Yu. S. Vershinina,&nbsp;V. M. Shkinev,&nbsp;M. A. Proskurnin","doi":"10.1134/S1061934826700541","DOIUrl":"10.1134/S1061934826700541","url":null,"abstract":"<p>A feedback-mode variant of thermal lens spectrometry was employed for steady-state photothermal measurements and time-resolved studies of thermal lens formation dynamics to compare the precision and sensitivity of measurements in aqueous polyethylene glycol (PEG) solutions of different molecular weights using a series of model systems (ferroin, cobalt complexes with nitrosonaphthols, and aqueous fullerene dispersions). The results of thermal lens measurements obtained in single-phase aqueous PEG solutions were compared with those obtained under extractive preconcentration conditions in PEG-based aqueous two-phase systems. Time-resolved thermal lens development curves in PEG-containing media were shown to exhibit greater stability and improved temporal separation between the thermal lens effect itself and the interfering thermophoresis phenomenon (the Soret effect) than those recorded in polymer-free aqueous media. To improve the accuracy of thermal diffusivity measurements, experimental conditions were selected under which the deviation from the theoretical curve development was minimal, and the Soret effect was absent (50–300 ms from the beginning of the measurement cycle). In PEG-containing media, the dynamics of thermal lens development showed better agreement with theory even for finely dispersed systems (aqueous fullerene dispersions), resulting in a substantial increase in the sensitivity of thermal lens measurements. The precision of thermal lens measurements in PEG solutions was higher than that in aqueous solutions because of lower signal fluctuations, which further enhanced the sensitivity of thermal lens measurements in such media. Conditions were proposed for the extraction-thermal lens determination of cobalt with 2-nitroso-1-naphthol based on the partitioning of the colored complex in an aqueous two-phase system composed of PEG and phosphate ions (K₂HPO₄, NaH₂PO₄, or NH₄H₂PO₄), followed by thermal lens determination in the extract phase. The detection limit for cobalt with 2-nitroso-1-naphthol in the phosphate–PEG aqueous system was 1 μmol/L (532 nm, excitation laser power 10 mW), whereas the detection limit for cobalt with nitroso-R salt in the ammonium sulfate–PEG aqueous system (excitation laser power 100 mW) was 0.2 μmol/L. For aqueous fullerene dispersions, a twofold increase in measurement sensitivity was demonstrated compared with aqueous media without PEG.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1133 - 1145"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Doxorubicin in the Presence of Bortezomib in Blood Plasma Based on Fluorescence Quenching of Quantum Dots","authors":"S. A. Meshcheryakova,&nbsp;V. V. Olomskaya,&nbsp;D. D. Drozd,&nbsp;O. A. Goryacheva,&nbsp;I. Yu. Goryacheva","doi":"10.1134/S1061934826700565","DOIUrl":"10.1134/S1061934826700565","url":null,"abstract":"<p>A procedure for determining doxorubicin in the presence of bortezomib in model aqueous solutions and blood plasma is developed based on the fluorescence quenching of CdZnSeS/ZnS and AgInS/ZnS quantum dots. Since doxorubicin is often used in combination chemotherapy with bortezomib, monitoring its plasma concentration is important for increasing the effectiveness of chemotherapy and reducing the risk of side effects. The sensitivity of doxorubicin determination in the presence of bortezomib (0.3 μM) is compared using quantum dots with different surface ligands. A trend toward decreased fluorescence quenching efficiency of quantum dots by doxorubicin in the presence of bortezomib is demonstrated. The use of alloyed CdZnSeS/ZnS quantum dots stabilized with 3-mercaptropropionic acid for the determination of doxorubicin in the presence of bortezomib in plasma is demonstrated, with a detection limit of 0.08 μM and a quantification limit of 0.24 μM.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1152 - 1162"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality by Design Approach for the Development of a Rapid Stability-Indicating Spectrophotometric Method for the Estimation of Amphotericin B in Bulk and Formulations","authors":"Sanjana S. Prakash,&nbsp;Shivakumar Hagalvadi Nanjappa,&nbsp;Vanita Somasekhar,&nbsp;Rushikesh Shinde,&nbsp;Avichal Kumar","doi":"10.1134/S1061934825603202","DOIUrl":"10.1134/S1061934825603202","url":null,"abstract":"<p>This study presents the first application of an analytical quality by design approach using face-centered central composite design to optimize an ultraviolet-visible spectrophotometric method for the estimation of amphotericin B (<b>Amp B</b>). The optimal critical method parameters established using the desirability approach indicated a detection wavelength of 385 nm in a solvent of 0.1 N HCl (pH 1.2), which significantly improved (<i>p</i> &lt; 0.05) the extinction coefficient while minimizing aggregation. The method, when validated as per ICH Q2(R2), displayed high linearity (<i>R</i>² = 0.997) in the concentration range from 6–36 µg/mL. The method was found to display high accuracy, with recoveries ranging from 95.41 ± 0.02% to 97.77 ± 0.13%, and high precision (relative standard deviation &lt; 2%) for intra- and inter-day studies. The limit of detection and limit of quantification were found to be 4.75 and 14.40 µg/mL, respectively. The method was found to be highly specific for Amp B as it ruled out the possibility of any interference by excipients, and was quite robust and reproducible across analysts and conditions. The method was able to successfully assess the improved thermal stability, reduced aggregation, and therefore the decreased irritation potential of the nano-vesicular formulation compared to the marketed product. The validated method is simple, rapid, cost-effective, and accurate for routine quantification of Amp B, and it offers a valuable in vitro alternative tool for assessing ocular irritancy potential.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1217 - 1235"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensor Surfaces Based on Modified Silver Nanofilms for the Determination of Phosphates in Water by Surface-Enhanced Raman Scattering","authors":"I. K. Solontsov,&nbsp;M. V. Samodelova,&nbsp;O. O. Kapitanova,&nbsp;I. A. Veselova","doi":"10.1134/S1061934826700528","DOIUrl":"10.1134/S1061934826700528","url":null,"abstract":"<p>A sensitive approach to the determination of trace amounts of phosphate ions in aqueous solutions using surface-enhanced Raman spectroscopy on silver nanofilms modified with cetyltrimethylammonium bromide was proposed. The role of a surface modifier in enhancing the analytical signal was investigated. For pure sodium hydrogen phosphate solutions, the detection limit was 60 nM (5.7 μg/L), and the calibration function was linear over the hydrogen phosphate ion concentration range of 1 × 10^–7 to 1 × 10^–4 M.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1118 - 1125"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Low-Cost Method for Determining the Quantitative Chemical Composition of Slag Wool","authors":"B. Fernández-Arguijo,&nbsp;J. E. Vilasó-Cadre,&nbsp;A. Ramírez-Rodríguez,&nbsp;I. A. Reyes-Domínguez,&nbsp;J. Aguilar-Carrillo,&nbsp;E. F. Armendáriz-Alonso,&nbsp;R. Cruz,&nbsp;L. A. Guerra-Fuentes,&nbsp;R. I. Hernández-Molinar,&nbsp;E. J. Gutiérrez-Castañeda","doi":"10.1134/S1061934825601926","DOIUrl":"10.1134/S1061934825601926","url":null,"abstract":"<p>An important issue to consider when determining the chemical composition of slag wool (<b>CCSW</b>) is sample digestion due to the complexity of the matrix. Some methods usually employed to determine the composition of silicate rocks and minerals have been adapted to determine CCSW. However, the dissolution characteristics between rock/glass and slag wool fibers are rather different and thus affect quantitative analysis. The present work reports a reliable, quantitative, low-cost method for the determination of CCSW based on sample fusion, acid dissolution, and subsequent analysis by flame atomic absorption spectrometry. Some oxides were analyzed by gravimetry, allowing the overall chemical characterization of slag wool. Scanning electron microscopy/energy dispersive X-ray spectrometry and traditional current methods were performed for comparison. A study regarding the effects of sample amount and particle size is presented. The results show that the proposed method allows determining CCSW with accuracy; in addition, it offers the advantage of reducing both the melting temperature and the sample preparation time, representing a lower-cost analytical method for the determination of CCSW.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"81 :","pages":"1261 - 1271"},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}