Journal of Analytical Chemistry最新文献

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Gas-Chromatographic Determination of the Concentration of the Organochlorine Pesticide Lindane in Some Samples of Agricultural Products during Primary and Secondary Cultivation in the Same Soil 气相色谱法测定同一土壤一、二次栽培中部分农产品中有机氯农药林丹的浓度
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825700522
D. E. Musabirov, R. A. Daukaev, D. O. Karimov, E. N. Usmanova, E. E. Zelenkovskaya, V. Yu. Guskov
{"title":"Gas-Chromatographic Determination of the Concentration of the Organochlorine Pesticide Lindane in Some Samples of Agricultural Products during Primary and Secondary Cultivation in the Same Soil","authors":"D. E. Musabirov,&nbsp;R. A. Daukaev,&nbsp;D. O. Karimov,&nbsp;E. N. Usmanova,&nbsp;E. E. Zelenkovskaya,&nbsp;V. Yu. Guskov","doi":"10.1134/S1061934825700522","DOIUrl":"10.1134/S1061934825700522","url":null,"abstract":"<p>The residual concentration of the organochlorine pesticide lindane in model samples of tomatoes, cucumbers, and onions is studied by gas chromatography. A pesticide solution was added to soil, after which these plants were grown twice in the contaminated soil. Two sample preparation methods were used in the analysis: according to GOST 30349 and a similar procedure using liquid nitrogen at the homogenization stage. It was found that the use of liquid nitrogen in the sample preparation increased the recovery of the analytes by up to two times. For tomato and cucumber samples, it was found that both during primary and secondary plant cultivation, the residual amount of organochlorine pesticide increased with increasing pesticide concentration in spraying. However, this is not typical for onion samples. In the last case, the residual amounts of lindane decreased with increasing concentration of the initial analyte. Such behavior is typical for root crop samples, which do not include onion. The residual concentration of lindane in different parts of tomato and cucumber plants varied: tomato accumulated the pesticide to a greater extent in the fruit, and cucumber, in the tops. In re-cultivation in contaminated soil, the residual amount of lindane did not exceed 12% of the initial one. As a result, it was found that the most suitable plant for growing on lindane-contaminated soils is cucumber, because 80–90% of lindane is accumulated in inedible cucumber roots, and the fruits contain the smallest amount of the pollutant among all vegetables. Tomato is unsuitable for growing on contaminated soils, because the fruits accumulate significant amounts of lindane.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1226 - 1234"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145168785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New Micellar Polymers as Polyfunctional Modifiers of Electrophoretic Systems 新型胶束聚合物作为电泳体系的多功能改性剂
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825700571
A. A. Adamova, D. A. Potapenko, P. A. Fetin, L. A. Kartsova
{"title":"New Micellar Polymers as Polyfunctional Modifiers of Electrophoretic Systems","authors":"A. A. Adamova,&nbsp;D. A. Potapenko,&nbsp;P. A. Fetin,&nbsp;L. A. Kartsova","doi":"10.1134/S1061934825700571","DOIUrl":"10.1134/S1061934825700571","url":null,"abstract":"<p>New modifiers of electrophoretic systems—cationic polyelectrolytes with micellar properties, i.e. poly-11-acryloyloxyundecyl-<i>N</i>-methylpiperidinium bromide (<b>pAUMP-Br</b>), poly-11-acryloyloxyundecylpyridinium bromide, poly-11-acryloyloxyundecyl-1,4-diazabicyclo[2.2.2]octane-1-ium bromide, and a chiral copolymer based on acylated quinine and <i>N</i>-(11-acryloyloxyundecyl)-<i>N</i>-methylpiperidinium bromide (<b>pAUMP–AQin</b>)—have been synthesized. The electrophoretic properties of these polymers have been studied in the separation model mixtures of biologically active substances, steroid hormones, biogenic amines, and amino acids, in micellar electrokinetic chromatography and capillary electrochromatography modes. It was found that the new polyelectrolytes are polyfunctional modifiers of quartz capillary walls and the background electrolyte and ensure the implementation of various modes of electrophoretic separation of biologically active substances, indirect detection in the presence of a chromophore group in the polymer, generation of an anodic electroosmotic flow, and governing the efficiency and selectivity of the analyte separation. Field-amplified sample stacking in a quartz capillary modified with pAUMP-Br was performed, which ensured the 15-fold preconcentration of biogenic amines and reduced the limits of detection. It was found that the pAUMP–AQin copolymer with a chiral label favors the separation of tryptophan enantiomers.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1292 - 1300"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficient Approach to the Immobilization of Glucose Oxidase in Sol-Gel Polymeric Matrices Using Silane Precursors with One and Two Non-Hydrolyzable Bonds 用硅烷前驱体固定化葡萄糖氧化酶在溶胶-凝胶聚合物基质中的高效方法
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825700583
O. A. Kamanina, E. A. Lantsova, P. V. Rybochkin
{"title":"Efficient Approach to the Immobilization of Glucose Oxidase in Sol-Gel Polymeric Matrices Using Silane Precursors with One and Two Non-Hydrolyzable Bonds","authors":"O. A. Kamanina,&nbsp;E. A. Lantsova,&nbsp;P. V. Rybochkin","doi":"10.1134/S1061934825700583","DOIUrl":"10.1134/S1061934825700583","url":null,"abstract":"<p>The objective of this study is to utilize novel hybrid biomaterials, comprising glucose oxidase coated with an alkyl-modified silica polymer shell in conjunction with a Clark oxygen electrode, as biocatalysts. This work studied the efficiency of immobilization of glucose oxidase in a sol-gel matrix based on the silane precursors tetraethoxysilane, methyltriethoxysilane, and diethoxydimethylsilane. The use of a 50/50 vol % mixture of methyltriethoxysilane and tetraethoxysilane produces a polymer material with smaller pores compared to a mixture of other silane precursors. When precursors are added during the formation of the material with non-hydrolyzed bonds, the surface area of the materials increases from 40 m<sup>2</sup>/g when using only tetraethoxysilane to 70 m<sup>2</sup>/g with a mixture of diethoxydimethylsilane and tetraethoxysilane, and up to 110 m<sup>2</sup>/g with a mixture of methyltriethoxysilane and tetraethoxysilane. In addition, adding diethoxydimethylsilane to the tetraethoxysilane system increases the pore volume from 0.008 to 0.09 cm<sup>3</sup>/g, and adding methyltriethoxysilane increases the pore volume to 0.33 cm<sup>3</sup>/g. A biosensor using glucose oxidase immobilized in a polymer matrix based on a diethoxydimethylsilane/tetraethoxysilane 50/50 vol % mixture is characterized by a high sensitivity coefficient of 3.8 mg O<sub>2</sub>/min mmol and a glucose concentration range of 1–280 mmol/L. Therefore, this study demonstrates that the use of polymers with linear fragments is more effective for the immobilization of glucose oxidase compared to traditional immobilization methods. This could lead to the development of new and efficient enzyme immobilization methods for the determination of individual substances in biological fluids, e.g., blood glucose.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1301 - 1312"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of Quercetin in Pharmaceuticals by Digital Colorimetry Using Assemblable Microfluidic Devices Based on Paper Modified with Gold and Silver Nanoparticles 基于金、银纳米粒子修饰的可组装微流控装置的数字比色法测定药物中的槲皮素
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825700480
A. A. Furletov, A. V. Yakimenko, V. V. Apyari, S. G. Dmitrienko, I. I. Torocheshnikova
{"title":"Determination of Quercetin in Pharmaceuticals by Digital Colorimetry Using Assemblable Microfluidic Devices Based on Paper Modified with Gold and Silver Nanoparticles","authors":"A. A. Furletov,&nbsp;A. V. Yakimenko,&nbsp;V. V. Apyari,&nbsp;S. G. Dmitrienko,&nbsp;I. I. Torocheshnikova","doi":"10.1134/S1061934825700480","DOIUrl":"10.1134/S1061934825700480","url":null,"abstract":"<p>One of current areas of application of microfluidic paper-based analytical devices (<b>μPADs</b>) is the determination of biologically active substances in various samples, including pharmaceuticals. Such determination is often carried out as screening analysis to identify samples that should be examined in more detail by highly informative, but relatively expensive methods. In this paper, an original method for the colorimetric determination of quercetin is proposed using microfluidic analytical systems based on paper modified with gold and silver nanoparticles of various morphologies. It is based on the reduction of silver(I) ions to metallic silver under the action of quercetin with a contrasting change of the color of the μPADs detection zones. A possibility of using a monitor calibrator and a smartphone camera for recording the analytical signal is demonstrated. Optimal conditions for the analysis are selected. It is shown that the type of nanoparticles affects the sensitivity of quercetin determination, which offers promise for creating multisensor systems for discriminating complex samples. The limits of detection for quercetin under the selected conditions are 70–120 ng, depending on the nature of the analytical reagent and the method of recording the analytical signal. The analytical range is 2–10 μg. The sample volume sufficient for analysis does not exceed 25 μL. The selectivity of the proposed method for determining quercetin with respect to a number of common inorganic ions and organic substances is assessed. The applicability of the developed approach to determining quercetin in three pharmaceutical preparations is shown.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1170 - 1181"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of High-Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry to the Assessment of Acrylamide Contamination of Roasted Coffee Beans 高效液相色谱-串联三重四极杆质谱法在烘焙咖啡豆丙烯酰胺污染评价中的应用
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825600076
Dinh Vu Le, Nguyen Thi Bich Phuong, Van Trong Nguyen
{"title":"Application of High-Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry to the Assessment of Acrylamide Contamination of Roasted Coffee Beans","authors":"Dinh Vu Le,&nbsp;Nguyen Thi Bich Phuong,&nbsp;Van Trong Nguyen","doi":"10.1134/S1061934825600076","DOIUrl":"10.1134/S1061934825600076","url":null,"abstract":"<p>High-performance liquid chromatography-tandem triple quadrupole mass spectrometry was validated to analyze acrylamide in coffee samples. Under optimal conditions, the quantitative range for acrylamide was 50–1000 µg/L, and the method detection limit was 30 µg/L. The recovery ranged from 85.2 to 97.3%, with high repeatability and reproducibility. The measurement uncertainty of the method was calculated using a top-down approach and determined to be 12.3%. The validated method was then applied to analyze 68 samples of roasted coffee beans collected from markets in Ho Chi Minh City. The results showed that 51.5% of the samples contained acrylamide at concentrations ranging from 51 to 560 µg/kg, and 10.3% had acrylamide contamination exceeding the maximum acceptance level. The results also demonstrated that the acrylamide contamination level in medium-roast coffee was much higher than that in light-roast and dark-roast coffee. The observation indicates that the level of acrylamide contamination of roasted coffee sold in Ho Chi Minh City markets is an excellent concern for food safety and quality management.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1256 - 1263"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Comprehensive Review of Analytical Methodologies for Memantine Hydrochloride 盐酸美金刚的分析方法综述
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825600489
Hardik L. Varu, Hitesh N. Parmar, Harshad D. Vadhel, Mrunal A. Ambasana
{"title":"A Comprehensive Review of Analytical Methodologies for Memantine Hydrochloride","authors":"Hardik L. Varu,&nbsp;Hitesh N. Parmar,&nbsp;Harshad D. Vadhel,&nbsp;Mrunal A. Ambasana","doi":"10.1134/S1061934825600489","DOIUrl":"10.1134/S1061934825600489","url":null,"abstract":"<p>The present review provides a comprehensive overview of the development of analytical methodologies that are well-developed and validated for the estimation of memantine. Memantine is an N-methyl-D-aspartate receptor and is widely used for dementia, Parkinson’s disease, pervasive developmental disorders, schizophrenia, and alcohol abuse disorder. However, the estimation of memantine continues to attract researchers' attention due to its unique chemical properties. Memantine is a saturated compound belonging to biopharmaceutics classification system Class I, due to its high solubility and permeability. However, its lack of unsaturation makes direct detection using ultraviolet-visible (<b>UV-Vis</b>) spectroscopy challenging. Consequently, various analytical approaches have been developed and validated for the detection of memantine, including UV-Vis spectroscopy, fluorescence spectroscopy, infrared spectroscopy, voltammetry, potentiometry, liquid chromatography, gas chromatography, nuclear magnetic resonance spectroscopy, and other hyphenated techniques. This paper provides comprehensive insights for analysts interested in developing analytical methods.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1125 - 1139"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sample Preparation of Large Mass Black Shale Samples Using Ammonium Bifluoride for ICP–MS/AES Instrumental Analysis 双氟化铵制备大质量黑色页岩样品用于ICP-MS /AES仪器分析
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825700510
V. P. Kolotov, V. I. Kazin, E. A. Zakharchenko, I. N. Gromyak
{"title":"Sample Preparation of Large Mass Black Shale Samples Using Ammonium Bifluoride for ICP–MS/AES Instrumental Analysis","authors":"V. P. Kolotov,&nbsp;V. I. Kazin,&nbsp;E. A. Zakharchenko,&nbsp;I. N. Gromyak","doi":"10.1134/S1061934825700510","DOIUrl":"10.1134/S1061934825700510","url":null,"abstract":"<p>A procedure is developed for acid digestion of black shale samples weighing up to 1000 mg in an open system. The procedure involves the destruction of the mineral matrix with a fivefold excess of ammonium bifluoride at 230°C for 6 h, followed by the reagent removal through distillation and the subsequent treatment of the residue with a mixture of perchloric and nitric acids to achieve the complete dissolution. The resulting solution remains stable over time and serves as the basis for the direct determination of more than 60 elements using a combination of ICP–MS and ICP–AES techniques. The method enables the quantification of gold, silver, and rhodium directly in the post-decomposition solution with the computational correction of spectral interferences. The method was tested on certified reference black shale materials from the Baikal–Vitim province SLg-1 and SChS-1 (Sukhoi Log deposit, Irkutsk district, Russia). The study also presents analytical results for their updated counterparts, SLg-1A and SChS-1A.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1212 - 1225"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resonance Rayleigh Scattering and Spectrofluorimetric Sensing of 6-Mercaptopurine Using PbS Quantum Dot–Glutathione Nanocomposites PbS量子点-谷胱甘肽纳米复合材料对6-巯基嘌呤的共振瑞利散射和荧光光谱检测
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825700509
Abdolmohsen Amouri, Farzaneh Marahel, Alireza Geramizadegan, Mohammad Reza Asghari Ganjeh
{"title":"Resonance Rayleigh Scattering and Spectrofluorimetric Sensing of 6-Mercaptopurine Using PbS Quantum Dot–Glutathione Nanocomposites","authors":"Abdolmohsen Amouri,&nbsp;Farzaneh Marahel,&nbsp;Alireza Geramizadegan,&nbsp;Mohammad Reza Asghari Ganjeh","doi":"10.1134/S1061934825700509","DOIUrl":"10.1134/S1061934825700509","url":null,"abstract":"<p>In this study, a resonance Rayleigh scattering (<b>RRS</b>) technique and a spectrofluorimetric method were developed using a PbS quantum dot–glutathione nanocomposite sensor for the detection of 6-mercaptopurine in real samples. The sensor was characterized using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The scattering intensity (∆<i>I</i><sub>RRS</sub>) signal was detected using a fluorescence detector at an excitation wavelength of 345 nm. The analytical performance of the coupled system demonstrated a linear response to 6-mercaptopurine over a concentration range of 0.05–200.0 ng/mL. The method exhibited a relative standard deviation of ±1.1% and a detection limit (3 s/m) of 0.05 ng/mL, with a response time of 60 s in a Britton – Robinson buffer solution. Furthermore, the PbS quantum dot–glutathione nanocomposite sensor, based on the RRS technique, was successfully applied for the determination of 6-mercaptopurine in samples. The proposed method could be used for the analysis of pharmaceutical formulations, human biological fluids, and hospital samples for the detection of different drugs.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1203 - 1211"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Voltammetric Sensor Based on Tin Dioxide Nanoparticles for the Determination of Taxifolin 二氧化锡纳米颗粒伏安传感器测定紫杉醇
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825700558
G. K. Ziyatdinova, A. D. Tarabukina
{"title":"A Voltammetric Sensor Based on Tin Dioxide Nanoparticles for the Determination of Taxifolin","authors":"G. K. Ziyatdinova,&nbsp;A. D. Tarabukina","doi":"10.1134/S1061934825700558","DOIUrl":"10.1134/S1061934825700558","url":null,"abstract":"<p>A voltammetric sensor for the determination of taxifolin is developed based on a glassy carbon electrode (GCE) modified with tin dioxide nanoparticles (SnO<sub>2</sub> NPs) dispersed in <i>N</i>-hexadecylpyridinium bromide. The modification of the electrode surface enhances the reversibility of the electrochemical reaction and significantly increases redox currents compared to an unmodified GCE, by factors of 2.3 and 3.3 for the anodic and cathodic peaks, respectively. Scanning electron microscopy reveals a uniformly distributed layer of spherical SnO<sub>2</sub> NPs on the electrode surface; their diameters range from 20 to 40 nm. This modification results in a 3.9-fold increase in the electrode’s effective surface area and a 143-fold enhancement in the heterogeneous electron transfer rate constant. It was found that the electrooxidation of taxifolin involves protons. The electrode reaction follows a mixed control mechanism including both diffusion and adsorption components. Under differential pulse voltammetry conditions, using Britton–Robinson buffer of pH 6.0, the sensor detects taxifolin over a concentration range of 0.075 to 25 μM, with a limit of detection of 70.7 nM. These analytical parameters are comparable to those achieved by other electrochemical techniques; however, the proposed method offers greater simplicity and higher speed, and does not require complex electrode modification. The sensor demonstrated successful application to the analysis of dietary supplements containing dihydroquercetin (taxifolin). The results aligned with those obtained by coulometric titration using electrochemically generated bromine.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1264 - 1273"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Catalytic Hairpin Assembly-Driven Quadruped DNA Walker for the Ratiometric Electrochemical Determination of Mucin 1 催化发夹驱动的四足DNA步行者用于黏液蛋白1的比例电化学测定
IF 1.1 4区 化学
Journal of Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1134/S1061934825600611
Zhimin Guan, Yaoyao Xie, Ke Li, Junling An, Ping Wang
{"title":"Catalytic Hairpin Assembly-Driven Quadruped DNA Walker for the Ratiometric Electrochemical Determination of Mucin 1","authors":"Zhimin Guan,&nbsp;Yaoyao Xie,&nbsp;Ke Li,&nbsp;Junling An,&nbsp;Ping Wang","doi":"10.1134/S1061934825600611","DOIUrl":"10.1134/S1061934825600611","url":null,"abstract":"<p>A ratiometric electrochemical aptasensor for the sensitive detection of mucin 1 (<b>MUC1</b>) was designed based on a quadruped DNA walker driven by catalytic hairpin assembly (<b>CHA</b>). With the tetrahedral DNA nanostructure as the framework, the four legs that extend out acted as the walking chains. MUC1 bound specifically to the aptamer, activating the quadruped DNA walker and initiating the CHA reaction. The activated quadruped DNA walker hybridized with hairpin DNA1 labeled with methylene blue (<b>MB-HP1</b>) immobilized on the electrode surface, and the toehold area of the MB-HP1 probe was exposed to bind with hairpin DNA2 labeled with ferrocene (<b>Fc-HP2</b>), causing the quadruped DNA walker to be released. It continued to move on the electrode surface and bound with another MB-HP1. Eventually, a large number of electrochemical signal molecules were brought close to the electrode surface, while MB moved farther away. Under optimal conditions, the peak current ratio (<i>I</i><sub>Fc</sub>/<i>I</i><sub>MB</sub>) was linearly correlated with the logarithm of MUC1 concentration from 1 fg/mL to 1 ng/mL, with a detection limit as low as 0.56 fg/mL. In addition, the sensing platform demonstrated good performance in the analysis of serum samples.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 7","pages":"1274 - 1282"},"PeriodicalIF":1.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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