Regularities of Retention of Alcohols, Ketones, and Sugars on a Sulfonic Acid Cation Exchanger in H+-, Ca2+- and La3+-Forms

Abstract

The chromatographic retention of alcohols, ketones, alditols, and carbohydrates on a cation exchanging column (200 × 4.6 mm, Sevko AA) packed with sulfonated poly(styrene-divinylbenzene) with a crosslinking degree of 10% and a particle diameter of 7 μm saturated with various counterions (H⁺, Ca²⁺ and La³⁺) is studied in the ligand exchange chromatography version. The sorbent in the Ca²⁺ form shows higher retention times and selectivity in the separation of carbohydrates and ethylene glycols. The sorbent in the La³⁺ form provides a strong retention and a better selectivity for the separation of alditols. A possibility of using this column for determining carbohydrates in food products (juice, honey) using refractometric detection is shown.