卫生研究最新文献

{"title":"[A nested case-control study on dyslipidemia in Chinese adults of 10 provinces based on gut microbiota and targeted lipid metabolomics].","authors":"Yifei Ouyang, Xiaofan Zhang, Fangxu Guan, Jiguo Zhang, Wenwen Du, Feifei Huang, Xiaofang Jia, Li Li, Chang Su, Huijun Wang, Bing Zhang","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.004","DOIUrl":"10.19813/j.cnki.weishengyanjiu.2025.05.004","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanisms by which the interaction between gut microbiota and host lipid metabolites affects dyslipidemia.</p><p><strong>Methods: </strong>Utilizing data from the China Health and Nutrition Survey(2018) and the \"China Development and Nutrition Health Impact Cohort Study\"(2022-2023), a nested case-control study design was employed. A total of 229 newly diagnosed dyslipidemia patients(case group) and 229 normolipidemic individuals(control group) were enrolled through 1∶1 matching by age and gender based on demographic information. Fasting blood samples collected were analyzed for serum lipid metabolic profiles using ultra-performance lipid chromatography-tandem mass spectrometry(UPLC-MS/MS), and fecal samples were subjected to 16 S rRNA high-throughput sequencing to analyze the structure of gut microbiota. Univariate analyses were performed to identify differentially expressed metabolites and microbial taxa between groups, followed by Spearman correlation analysis and conditional logistic regression modeling to explore the impact of microbiota-metabolite interactions on dyslipidemia.</p><p><strong>Results: </strong>A total of 18 differential lipid metabolites(9 upregulated, 9 downregulated) and 5 significant bacterial taxa were identified: Clostridium sensu stricto 1, Muribaculaceae, Holdemanella, Veillonella, and Faecalitalea. Correlation analysis revealed significant associations between 2 bacterial taxa and 5 metabolites. Conditional logistic regression indicated that increased abundance of Clostridium sensu stricto 1 was associated with a reduced risk of dyslipidemia(OR=0.69, 95%CI 0.41-1.50, P=0.04), while elevated levels of triglyceride TG(15:0＿18:2＿18:2) significantly increased the risk of developing the condition(OR=1.94, 95%CI 1.08-3.49, P=0.03).</p><p><strong>Conclusion: </strong>Clostridium sensu stricto 1 may reduce the risk of dyslipidemia by lowering the level of the lipid metabolite triglyceride(15:0＿18:2＿18:2).</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"732-737"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Ginseng adventitious root extract ameliorates high glucose-induced HK-2 cells injury by regulating the NLRP3 inflammasome signaling pathway].","authors":"Wenli Hu, Bingzhong Zhai, Bing Zhu","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.018","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.018","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether ginseng adventitious root extract(GARE)exerts protective effects against high glucose(HG)-induced injury in HK-2 cells and to elucidate the underlying inflammation-related molecular mechanisms.</p><p><strong>Methods: </strong>GARE was obtained via the hot reflux method. Human kidney 2(HK-2) cells were cultured and divided into the following groups: control(5.5 mmol/L glucose), high glucose(HG, 35 mmol/L glucose) and HG+GARE(0.1 and 1 μg/mL GARE). Cells were treated for 24 hours.</p><p><strong>Results: </strong>Treatment with GARE at concentrations of 0.1 and 1 μg/mL significantly improved the viability of HK-2 cells. Under HG conditions, the NLRP3 inflammasome pathway in HK-2 cells was activated, increasing the expression of inflammation and fibrosis related factors, including IL-18, IL-1β and Col Ⅰ. However, These alterations were reversed following GARE treatment.</p><p><strong>Conclusion: </strong>GARE may ameliorates HG-induced HK-2 cell injury by regulating the NLRP3 inflammasome signaling pathway and suppressing the expression of key inflammatory factors.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"827-832"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Impact of high-fat diet on intestinal fat absorption and fatty acid metabolism in rats transplanted with gut microbiota from hypertriglyceridemic individuals].","authors":"Yan Ma, Jian Huang, Qin Zhuo, Shi Shen, Bing Zhang, Huijun Wang, Jiguo Zhang, Ou Wang","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.008","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.008","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of fecal microbiota transplantation on fat absorption rate, fatty acid metabolic flux, and short-chain fatty acid production in germ-free Sprague-Dawley(SD) rats.</p><p><strong>Methods: </strong>Ten-week-old male germ-free SD rats were randomly grouped(5-6 rats/group) by body weight. Blank control: saline gavaged + germ-free basal diet. Normal control: colonized with normal-TG(≤1.7 mmol/L) human gut microbiota + germ-free basal diet. Two other groups: colonized with high-TG(&gt;1.7 mmol/L) human gut microbiota, one on germ-free basal diet and the other on high-fat diet(40.5% fat energy). Day 0 marked colonization start. On Days 0, 2, 4, rats were gavaged with fecal bacteria(1 mL/100 g body weight). After 10-day adaptation, high-fat group started high-fat diet on Day 14. Feces were induced and collected aseptically on Days 0, 14, 21, 56, 77 for total fat(fat absorption rate), fatty acid profile, and short-chain fatty acids like butyric acid.</p><p><strong>Results: </strong>On Day 77, compared to blank control, normal control had lower body weight, fat absorption rate, and fecal palmitoleic and linoleic acids(P&lt;0.05), but higher butyric, palmitic, arachidic, oleic, DHA, and stearic acids(P&lt;0.05). High-TG group had higher body weight, fat absorption rate, and fecal palmitoleic and linoleic acids than normal control(P&lt;0.05), with lower butyric, three saturated, and oleic acids(P&lt;0.05). High-fat group had higher body weight, fat absorption rate, and fecal fatty acids than high-TG group(P&lt;0.05). After Day 21, α-linolenic acid was undetectable in all groups.</p><p><strong>Conclusion: </strong>In contrast to the positive regulation of lipid metabolism in rats by colonization with non-high-TG microbiota, colonization with high-TG microbiota disrupts lipid metabolism balance, inhibits the excretion of saturated fatty acids and oleic acid, reduces butyrate production, increases fat absorption rate, elevates the risk of obesity, and may synergize with a high-fat diet to exacerbate intestinal lipid metabolism disorders.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"757-762"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Relationship between physical activity and sleep quality in some university students of 6 provinces in 2022: The mediating role of perceived stress and the moderating role of gender].","authors":"Yi Wei, Yulan Zhou, Yanjie Liu, Zan Huang, Dongao Liu","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.014","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.014","url":null,"abstract":"<p><strong>Objective: </strong>To explore the relationship among physical activity(PA), perceived stress, gender, and sleep quality of university students.</p><p><strong>Methods: </strong>A total of 2338(925 male and 1413 female) university students of economics, management, education and science from one comprehensive undergraduate university in Guizhou, Shaanxi, Shandong, Hubei, Anhui and Zhejiang provinces were selected by convenient sampling method from October to December 2022. International Physical Activity Questionnaire, Pittsburgh sleep quality index, and Perceived Stress Scale were used to conduct a questionnaire survey of university students, and Bootstrap sampling test was used to conduct moderated mediation effect analysis.</p><p><strong>Results: </strong>The average age of the respondents was 19.25. The first to fourth grades have 732, 769, 462 and 375 students, respectively. PA was negatively correlated with sleep quality(r=-0.103, P&lt;0.01), and perceived stress was positively correlated with sleep quality(r=0.215, P&lt;0.01). Bootstrap test indicated that perceived stress played a mediating role between PA and sleep quality. The direct effect of PA on sleep quality of university students was-0.06. The mediating effect of perceived stress was-0.02, accounted for 27.63% of the total effect. The interaction term of perceived stress and gender had a statistically significant effect on the prediction of sleep quality(β=-0.05, P&lt;0.05), and gender played a moderating role in the prediction of sleep quality. The simple slope showed that with the increase of perceived stress, the sleep quality scores of males(β=0.30, P&lt;0.01) and females(β=0.20, P&lt;0.01) showed a significant upward trend, and the upward trend of males was more significant.</p><p><strong>Conclusion: </strong>The total amount of PA is related to better sleep quality. PA can improve the sleep quality of university students through the mediating effect of perceived stress and the moderating effect of gender. Compared with female students, the prediction effect of perceived stress on poor sleep quality of male students is more significant.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"798-804"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Multi-omics association among intestinal microbiome, metabolome, and histone H4 acetylation in children with asthma].","authors":"Ping Zheng, Chuanchuan Yu, Xifang Lyu, Qiang Wang","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.012","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.012","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the correlation between intestinal microbiome, metabolome and histone H4 acetylation level in children with asthma, and explore the mechanism that gut microbiome affects children with asthma.</p><p><strong>Methods: </strong>Histone H4 acetylation in induced sputum, 16S rRNA gene sequencing of gut microbiome, and untargeted fecal metabolomics were detected in asthmatic children and control children. Network method was used to analyze the correlation between characteristic gut microbes of asthma and histone H4 acetylation, Quantile g-computation(QGC)was used to analyze the effect of characteristic microbes on histone H4 acetylation, and the mediation model was used to analyze the mediating effect of metabolites in the process of gut microbes affecting histone H4 acetylation.</p><p><strong>Results: </strong>The mean A(optical density) value of histone H4 acetylation in sputum was 0.668±0.260 in asthmatic children(n=37), and 0.593±0.069 in control group(n=20). A total of 5 characteristic gut genera of children with asthma were significantly correlated with H4 acetylation level(adjusted P&lt;0.05). QGC analysis showed that the 5 characteristic gut genera had a positive effect on the histone acetylation level of histone H4(P=0.001), and the A value of histone H4 acetylation increased by 0.124 for every 1 quantile increase in the relative abundance of characteristic gut genera(95%CI 0.058-0.190). By spearman analysis, 34 fecal metabolites were significantly correlated with the five characteristic gut genera and histone H4 acetylation levels. Prevotella was found have influenced the level of H4 acetylation through the mediating effect of N-delta-acetylornithine, phenethylamine, 2-hydroxy-3-methylvalerate, alpha-ketoglutaramate, gamma-glutamylglycine, and mevalonolactone.</p><p><strong>Conclusion: </strong>The metabolites related with the characteristic gut bacteria in asthmatic children were associated with histone H4 acetylation, which suggested that the gut microbiome may induce childhood asthma by regulating epigenetic variation.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"784-804"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of gut microbiota from individuals with different triglyceride levels on hepatic and serum metabolomics in rats].","authors":"Hanchen Du, Bing Zhang, Ou Wang, Jian Huang, Xiyue Zhang","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.006","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.006","url":null,"abstract":"<p><strong>Objective: </strong>Analyze the differences in metabolites and metabolic pathways in the livers and serum of rats colonized with gut microbiota from individuals with different triglyceride(TG) levels.</p><p><strong>Methods: </strong>Using germ-free SD rats as the research subjects, the rats were colonized with gut microbiota from individuals with normal serum TG levels(≤1.7 mmol/L) and those with high TG levels(&gt;1.7 mmol/L), respectively. After the microbiota had colonized and grown for 12 weeks, the rats were sacrificed to obtain liver and serum samples for TG content analysis and non-targeted metabolomics analysis. Combined with multivariate statistical analysis and pathway enrichment methods, key metabolites and regulatory pathways were screened.</p><p><strong>Results: </strong>The liver and serum TG levels of rats colonized with gut microbiota from the normal TG population were 0.70 mg/mg and 0.32 mmol/L, respectively, while those colonized with gut microbiota from the high TG population were 1.07 mg/mg and 0.583 mmol/L, respectively. The differences were statistically significant(P&lt;0.05). A total of 64 and 60 differential metabolites were identified in the liver and serum samples of the two groups of rats, respectively. Among the top 20 differential metabolites ranked by the projection values of important variables in the liver and serum, compared with the normal TG group, in the high TG group, 13 triglycerides, 1 phosphatidylcholine, and 1 diglyceride were upregulated in the liver, while 1 cholesteryl ester, 1 phenylpyruvic acid, 1 tyrosine, and 2 diglycerides were downregulated. In the serum, 10 triglycerides, 5 phosphatidylcholines, 1 phosphatidylethanolamine, and 1 phosphatidylinositol were upregulated, while 1 phenylalanine and 1 aminooctanoic acid were downregulated. Metabolic pathway analysis indicated that phenylalanine, tyrosine, and tryptophan biosynthesis, glycerophospholipid metabolism, and phenylalanine metabolism were the main pathways causing metabolic differences, among which the glycerophospholipid metabolism pathway had an important impact on both the liver and serum.</p><p><strong>Conclusion: </strong>The gut microbiota may affect the host&apos;s lipid metabolism by regulating glycerophospholipid metabolism and other pathways.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"744-762"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Differential gut microbiota among adult residents of 15 provinces of China in 2018 and their relationship with blood lipids].","authors":"Fangxu Guan, Feifei Huang, Xiaofan Zhang, Liusen Wang, Jiguo Zhang, Xiaofang Jia, Wenwen Du, Yifei Quyang, Huijun Wang, Bing Zhang","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.001","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.001","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the characteristics and differences of gut microbiota in adult residents of 15 provinces of China under different inflammation levels, and to analyze their correlation with blood lipid levels.</p><p><strong>Methods: </strong>A total of 4158 adult residents who participated in the 2018 China Health and Nutrition Survey and completed all the surveys were selected. Biochemical tests were performed on serum samples, and 16S amplicon sequencing was carried out on fecal samples. According to the ultra-sensitive C-reactive protein, the population was divided into a low inflammation group and a high inflammation group. The uniformity and richness of bacterial species and the overall distribution characteristics of bacterial communities between the two groups were analyzed through diversity analysis, and the differences in bacterial communities between the two groups were analyzed using linear discriminant analysis. Finally, the differences in bacterial communities between the groups were analyzed in association with the physiological and biochemical indicators of the population.</p><p><strong>Results: </strong>Compared with the low inflammation group, the bacterial diversity in the high inflammation group was lower, and there was a significant difference in the overall bacterial composition between the two groups(R~2=0.25%, P=0.01). Six genera were enriched in the samples of the low inflammation group, and four genera were enriched in the high inflammation group. Among them, Lactobacillus and Streptococcus were positively correlated with BMI, and Romboutsia was positively correlated with TC, LDL-C and HDL-C. Clostridium UCG-014, Ruminococcus and Faecalibacterium were positively correlated with TG.</p><p><strong>Conclusion: </strong>The study found that there are differences in the overall structure and dominant bacteria of the gut microbiome in people with different inflammation levels, and the inflammatory response may have a certain impact on blood lipid levels through these dominant bacterial genera.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"707-714"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Relationship between hypertriglyceridemia and hypertension among adults aged 35-75 in Jiangsu Province from 2021 to 2023].","authors":"Yibin Gu, Shujun Gu, Qiuyi Zhang, Yu Qin, Lulu Chen, Jian Su, Lan Cui, Wencong Du, Jinyi Zhou","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.010","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.010","url":null,"abstract":"<p><strong>Objective: </strong>To understand the association between hypertriglyceridemia and hypertension in residents aged 35-75 in Jiangsu Province.</p><p><strong>Methods: </strong>Between May 26, 2021, and May 9, 2023, a comprehensive survey was carried out involving 81 499 permanent residents aged 35 to 75 across 22 districts and counties in 12 cities in Jiangsu Province. The survey encompassed questionnaires(detailing socioeconomic information, cardiovascular disease risk factors, etc. ), physical examinations(including height, weight, waist circumference, and blood pressure), and laboratory tests(such as fasting blood glucose and lipid profiles). A non-conditional logistic regression analysis was employed to investigate the relationship between hypertriglyceridemia and hypertension.</p><p><strong>Results: </strong>The participants had an average age of(54.37±10.29) years. Of these, 33 700 were male(41.4%) and 47 799 were female(58.6%). Urban residents made up 61.7% of the total participants. There were 14 241 individuals diagnosed with hypertriglyceridemia, reflecting a prevalence rate of 17.47%. Among those with hypertriglyceridemia, 7168 also had hypertension, indicating a prevalence rate of 50.33%(36.36%, χ~2 =966.18, P&lt;0.01), which was notably higher compared to individuals without hypertriglyceridemia. Upon adjusting for confounding variables such as age, gender, educational background, marital status, average annual household income, place of residence, smoking habits, alcohol consumption, hypercholesterolemia, elevated low-density lipoprotein cholesterol, reduced high-density lipoprotein cholesterol, diabetes, and being overweight or obese, it was determined that hypertriglyceridemia significantly increases the risk of developing hypertension(OR=1.472, 95%CI 1.414-1.533).</p><p><strong>Conclusion: </strong>The prevalence of hypertriglyceridemia among adults over the age of 35 in Jiangsu Province is high, and hypertriglyceridemia increases the risk of hypertension.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"770-783"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and validation of a prediction model for dyslipidemia in adults based on gut microbiota features of 4 provinces].","authors":"Wenwen Du, Weiwei Zhou, Fangxu Guan, Feifei Huang, Xiaofan Zhang, Jiguo Zhang, Xiaofang Jia, Chang Su, Yifei Ouyang, Huijun Wang, Bing Zhang","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.005","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.005","url":null,"abstract":"<p><strong>Objective: </strong>To develop and validate a 5-year dyslipidemia prediction model for adults aged ≥18 years based on gut microbiota data.</p><p><strong>Methods: </strong>Data were derived from the China Development and Nutrition Health Impact Cohort Study(formerly the China Health and Nutrition Survey, 2018-2023). We included 650 adults aged ≥18 years with baseline gut microbiota data and non-dyslipidemia. Key predictors of dyslipidemia were selected using LASSO regression, and a Cox proportional hazards regression model was constructed to assess dyslipidemia risk. Internal validation was performed using 1000 bootstrap resamples. Model performance was evaluated by the area under the receiver operating characteristic curve(AUC-ROC), Harrell&apos;s concordance index(C-index), and calibration curves.</p><p><strong>Results: </strong>The median(Q1, Q3) age of participants was 55.2(46.5, 65.9) years old. Over the 5-year follow-up, 138 individuals(21.2%) developed dyslipidemia. LASSO regression identified 30 key predictors, with 23 variables(e. g. , body mass index, Eubacterium xylanophilum group, and Ruminococcus gauvreauii group) retained in the final Cox model. The model demonstrated strong discriminative ability for 5-year dyslipidemia risk(AUC-ROC: 0.7843) and a bootstrap-corrected C-index of 0.6944. Calibration curves indicated high agreement between predicted and observed probabilities of dyslipidemia.</p><p><strong>Conclusion: </strong>This gut microbiota-based prediction model, developed from a nutrition-health cohort, exhibits robust performance and may provide basis for personalized and precise intervention.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"738-743"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Contamination and antimicrobial resistance of Salmonella from retail aquatic products in Beijing from 2023 to 2024].","authors":"Maosong Tian, Yujie Hu, Shiwei Liu, Bingyan Li, Menghan Li, Yongning Wu, Li Bai","doi":"10.19813/j.cnki.weishengyanjiu.2025.05.016","DOIUrl":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.05.016","url":null,"abstract":"<p><strong>Objective: </strong>To understand the contamination and antimicrobial resistance of Salmonella in aquatic products in Beijing.</p><p><strong>Methods: </strong>A total of 216 snails and shrimps were collected from wholesale markets, wet markets and supermarkets in Beijing from July 2023 to July 2024. Broth microdilution method was used for testing the antimicrobial susceptibility of Salmonella isolates against 18 antimicrobial drugs which belong to 11 categories, and whole genome sequencing and bioinformatics analysis were performed. The association between the drug resistant phenotype and the antibiotic resistance genes(ARGs) were analyzed.</p><p><strong>Results: </strong>In total, 24 Salmonella strains were detected from 15 samples, with a detection rate of 6.9%(15/216). There was no statistically significant difference among different seasons, sampling place types, and sample sources of the detection rate. Antimicrobial susceptibility result showed that the 24 strains had the highest resistance rate to tetracycline(54.2%, 13/24), followed by trimethoprim-sulfamethoxazole(50.0%, 12/24), chloramphenicol(50.0%, 12/24) and florfenicol(41.7%, 10/24), while all strains were susceptible to imipenem. The multi-drug resistance rate was 41.7%(10/24), and some isolates could be concurrent resistant to as much as 9 classes of antimicrobials. There were 10 drug resistance spectrums, and the dominant one was TET-CHL-FFC(n=6). The 24 isolates were classified into 11 serotypes, with S. Havana(n=5) being the dominant one, followed by S. Havana. Liverpool(n=3), S. Augustenborg(n=2), S. Agona(n=2), S. Thompson(n=2), S. Telelkebir(n=2), S. Indiana(n=2), S. Kentucky(n=2), S. Corvallis(n=2), S. Corvallis(n=2), S. Potsdam(n=1) and S. Goldcoast or S. Brikama(n=1), there was a sample that detected two serotypes simultaneously. Multi-locus sequence typing(MLST) result showed that ST1527 was the main type, accounting for 20.8%(5/24). Genomic analysis showed 56 distinct ARGs in 24 Salmonella isolates, which mainly carried tet(A), floR, sul2, mphA, tet(M) and so on, and found a S. Indiana carrying the colistin resistance gene mcr-1, respectively. The relationship between ARGs and drug-resistant phenotypes found that the Kappa value of clinically important drugs, including azithromycin, cefotaxime and ciprofloxacin all beyond 0.50, the prediction result of ARGs were well consistent with the resistant phenotype.</p><p><strong>Conclusion: </strong>Salmonella contamination existed in the retail link of aquatic products in Beijing, and the phenomenon of multi-drug resistance was serious, and there were many serotypes and drug resistance genes in isolates.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 5","pages":"811-819"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}