Critical Reviews in Eukaryotic Gene Expression最新文献_第2页

Cancer cell type dependent modifications of metastatic parameters by SLIT2-ROBO1 and RHOA cAMP signaling in response to TGFB1 and FGF2 癌细胞类型依赖于 SLIT2-ROBO1 和 RHOA cAMP 信号对 TGFB1 和 FGF2 转移参数的改变

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054055

Abdul Rauf Shakoori, Quratulain Amjad, Gary S. Stein, Andre van Wijnen, Abdul Rauf Shakoori

{"title":"Cancer cell type dependent modifications of metastatic parameters by SLIT2-ROBO1 and RHOA cAMP signaling in response to TGFB1 and FGF2","authors":"Abdul Rauf Shakoori, Quratulain Amjad, Gary S. Stein, Andre van Wijnen, Abdul Rauf Shakoori","doi":"10.1615/critreveukaryotgeneexpr.2024054055","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054055","url":null,"abstract":"The epithelial to mesenchymal transition (EMT) is a multistep process involving structural and functional alterations that are required for cancer metastasis, as well as loss of epithelial markers (e.g., E-cadherin/CDH1) and gain of mesenchymal markers (e.g., N-cadherin/CDH2, vimentin/VIM). Pathological events modify cell-cell interactions, cell-matrix adhesion and extra cellular matrix integrity leading to cell migration, evasion from the primary tumor and augmented invasiveness in the metastatic niche. This transformation is modulated by multiple paracrine factors (e.g., chemokines, growth factor), as well as SLIT2-ROBO1 signaling that collectively regulate expression of RHO GTPases (e.g., RHOA) and EMT marker genes. Yet, the roles of SLIT proteins in cancer remain enigmatic. In some cancer types, SLIT2 is anti-tumorigenic, while in other cancers it contributes towards the metastatic phenotype. Here we investigated the ambivalent metastatic activity of SLIT2 by analyzing how cAMP/RHOA signal transduction modulates SLIT-ROBO controlled metastatic parameters in response to the phosphodiesterase inhibitor IBMX (3-isobutyl-1-methylxanthine) and paracrine factors (TGF-β/TGFB1 and FGF2). Upon SLIT2 administration cell migration and proliferation increases in colon cancer cells and decreases in cervical cancer cells, while altering cell morphology and proliferation in both cancer types. These effects are reinforced by TGF-β/TGBF1 and FGF2, but attenuated by elevation of cAMP with IBMX, depending on the cancer cell type. Our data indicate that SLIT2 represents a potential biomarker for cancer diagnosis, prognosis, and therapy.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"15 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141551616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture alleviates Parkinson’s disease via promoting METTL9-catalyzed histidine methylation of NF-κB 电针通过促进 METTL9 催化的 NF-κB 组氨酸甲基化缓解帕金森病

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053243

Xiang Zhou, Liang Zhou, Jiayi Sun, Juan Zhang, Lei Sun

{"title":"Electroacupuncture alleviates Parkinson’s disease via promoting METTL9-catalyzed histidine methylation of NF-κB","authors":"Xiang Zhou, Liang Zhou, Jiayi Sun, Juan Zhang, Lei Sun","doi":"10.1615/critreveukaryotgeneexpr.2024053243","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053243","url":null,"abstract":"This study aimed to investigate the effects of electroacupuncture (EA) treatment on PD. MPTP administration was used establish PD mice model. The number of neurons was determined using TH staining. mRNA expression was detected using RT-qPCR. Protein expression was detected using western blot. Gene expression was determined using immunofluorescence and immunohistochemistry. The functions of neurons were determined using TUNEL and flow cytometry assay. The binding sites on NF-κB RELA in the promoter of NLRP3 was predicted by JASPAR and verified by luciferase and ChIP assays. EA treatment improved motor dysfunction in patients with PD. In vivo assays showed that MPTP administration induced the loss of neurons in mice, which was restored by EA treatment. Moreover, EA treatment attenuates motor deficits in MPTP-induced PD mice. EA treatment also inhibited the enrichment of pro-inflammatory cytokines and LDH, and suppressed neuronal pyroptosis. EA treatment increased the expression of METTL9. However, METTL9 deficiency dampened the effects of EA treatment and induced neuronal pyroptosis. Additionally, METTL9 promoted histidine methylation of NF-κB RELA, resulting the inhibition of epigenetic transcription of NLRP3. EA treatment restored neuronal function and improved motor dysfunction via promoting METTL9 histidine methylation of NF-κB/NLRP3 signaling.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"78 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141059103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foxm1-mediated transcriptional inactivation of NLRP3 inflammasome promotes immunosuppression in cervical cancer Foxm1 介导的 NLRP3 炎症小体转录失活可促进宫颈癌的免疫抑制

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053577

Weipeng Ji, Yang Jin, Wen Jiang

{"title":"Foxm1-mediated transcriptional inactivation of NLRP3 inflammasome promotes immunosuppression in cervical cancer","authors":"Weipeng Ji, Yang Jin, Wen Jiang","doi":"10.1615/critreveukaryotgeneexpr.2024053577","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053577","url":null,"abstract":"Foxm1 function as an oncogene in multiple human malignancies, including cervical cancer. However, the potential of Foxm1 in the tumor microenvironment (TME) is still unknown. The purpose of the present study is to investigate the role of Foxm1 in CD8+ T cell anti-tumor immunity. RT-qPCR is conducted to calculate mRNA levels. JASPAR is used to predict the binding sites between Foxm1 and NLRP3. ChIP assay is performed to verify the occupancy of Foxm1 on the promoter of NLRP3. Modulatory relationship between Foxm1 and NLRP3 is verified by luciferase assay. In vivo assays are conducted to further verify the role of Foxm1/NLRP3 axis in cervical cancer. HE staining assay is applied for histological analysis. Fow cytometry is conducted to determine the functions of immune cells. We found that Foxm1 knockdown decreases tumor burden and suppresses tumor growth of cervical cancer. Foxm1 knockdown promotes the infiltration of CD8+ T cells. Foxm1 deficiency inhibits the exhaustion of CD8+ T cells and facilitates the maintenance of CD8+ effector and stem-like T cells. Moreover, Foxm1 transcriptionally inactivates NLRP3 and suppressed the expression of innate cytokines IL-1β and IL-18. However, inhibition of NLRP3 inflammasome or neutralizing IL-1β and IL-18 inhibits anti-tumor immunity and promoted tumor growth in Foxm1 deficiency in CD8+ T cells. In summary, targeting Foxm1 mediates the activation of NLRP3 inflammasome and stimulates CD8+ T cell anti-tumor immunity in cervical cancer.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"62 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141166756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Palmdelphin inhibits ovarian cancer cell stem specification via downregulating ring finger protein 145 掌叶素通过下调环指蛋白145抑制卵巢癌细胞干的分化

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053542

Nanzi Xie, Sisi Yang, Changlan Dai, Wei Chen

引用次数: 0

Expression and clinicopathological significance of extracellular matrix remodeling markers in esophageal squamous carcinoma 细胞外基质重塑标记物在食管鳞癌中的表达及其临床病理学意义

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053646

Zhiqin Fan, Fei Chen, Yingmin Liu, Xiaotong Huang, Siyue Tian, Yuqing Ma

{"title":"Expression and clinicopathological significance of extracellular matrix remodeling markers in esophageal squamous carcinoma","authors":"Zhiqin Fan, Fei Chen, Yingmin Liu, Xiaotong Huang, Siyue Tian, Yuqing Ma","doi":"10.1615/critreveukaryotgeneexpr.2024053646","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053646","url":null,"abstract":"Esophageal squamous cell carcinoma (ESCC) is a common malignancy of the gastrointestinal tract with a single therapeutic option and a lack of effective clinical therapeutic biomarkers.Extracellular matrix (ECM) remodelling plays a pro-carcinogenic role in a variety of malignancies, but its role in esophageal squamous carcinoma remains to be elucidated.In this study, we examined the expression levels of ECM remodelling markers in 71 pairs of esophageal squamous carcinoma tissues and normal tissues adjacent to the carcinoma using immunohistochemical staining, and analysed their relationship with clinicopathological features and prognosis.The results suggested that extracellular matrix remodelling markers (Integrin αV, Fibronectin, MMP9) were abnormally highly expressed in esophageal squamous carcinoma tissues.There was a statistically significant difference between the positive expression of ECM remodelling and the TNM stage of esophageal squamous carcinoma, and there was no statistically significant correlation with age, gender and carcinoembryonic antigen expression, differentiation degree, T stage and lymph node metastasis.Overall survival rate and overall survival time were significantly lower in patients with positive ECM remodelling expression, which was an independent risk factor for poor prognosis of esophageal squamous carcinoma.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"41 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140884522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The alteration of the relationship between cells and their context is the basis of a pathological condition 细胞与其环境之间关系的改变是病理状态的基础

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053711

Domenico RIBATTI

引用次数: 0

Fundamentals and translational applications of stem cells and biomaterials in dental, oral and craniofacial regenerative medicine 干细胞和生物材料在牙科、口腔和颅面再生医学中的基础和转化应用

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-04-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053036

Yasaman Daneshian, Eric Lewallen, Amr Badreldin, Allan Dietz, Gary Stein, Simon Cool, Hyun-Mo Ryoo, Young Dan Cho, Andre van Wijnen

{"title":"Fundamentals and translational applications of stem cells and biomaterials in dental, oral and craniofacial regenerative medicine","authors":"Yasaman Daneshian, Eric Lewallen, Amr Badreldin, Allan Dietz, Gary Stein, Simon Cool, Hyun-Mo Ryoo, Young Dan Cho, Andre van Wijnen","doi":"10.1615/critreveukaryotgeneexpr.2024053036","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053036","url":null,"abstract":"Regenerative dental medicine continuously expands to improve treatments for prevalent clinical problems in dental and oral medicine. Stem cell based translational opportunities include regenerative therapies for tooth restoration, root canal therapy and inflammatory processes (e.g., periodontitis). The potential of regenerative approaches relies on the biological properties of dental stem cells. These and other multipotent somatic mesenchymal stem cell (MSC) types can in principle be applied as either autologous or allogeneic sources in dental procedures. Dental stem cells have distinct developmental origins and biological markers that determine their translational utility. Dental regenerative medicine is supported by mechanistic knowledge of the molecular pathways that regulate dental stem cell growth and differentiation. Cell fate determination and lineage progression of dental stem cells is regulated by multiple cell signaling pathways (e.g., WNTs, BMPs) and epigenetic mechanisms, including DNA modifications, histone modifications, and non-coding RNAs (e.g., miRNAs and lncRNAs). This review also considers a broad range of novel approaches in which stem cells are applied in combination with biopolymers, ceramics and composite materials, as well as small molecules (agonistic or anti-agonistic ligands) and natural compounds. Promising concepts in bone and dental tissue engineering continue to drive innovation in dental and non-dental restorative procedures.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"188 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammatory Markers Involved in the Pathogenesis of Dupuytren Contracture 参与杜普伊特伦挛缩症发病机制的炎症标志物

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-04-01 DOI: 10.1615/critreveukaryotgeneexpr.2024052889

William Cates, Janet Denbeigh, Ralph Salvagno, Sanjeev Kakar, Andre van Wijnen, Charles Eaton

{"title":"Inflammatory Markers Involved in the Pathogenesis of Dupuytren Contracture","authors":"William Cates, Janet Denbeigh, Ralph Salvagno, Sanjeev Kakar, Andre van Wijnen, Charles Eaton","doi":"10.1615/critreveukaryotgeneexpr.2024052889","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024052889","url":null,"abstract":"Dupuytren Disease is a common fibroproliferative disease that can result in debilitating hand deformities. Partial correction and return of deformity are common with surgical or clinical treatments at present. While current treatments are limited to local procedures for relatively late effects of the disease, the pathophysiology of this connective tissue disorder is associated with both local and systemic processes (e.g., fibrosis, inflammation). Hence, a better understanding of the systemic circulation of Dupuytren related cytokines and growth factors may provide important insights into disease progression. In addition, systemic biomarker analysis could yield new concepts for treatments of Dupuytren that attenuate circulatory factors (e.g., anti-inflammatory agents, neutralizing antibodies). Progress in the development of any disease modifying biologic treatment for Dupuytren has been hampered by the lack of clinically useful biomarkers. The characterization of nonsurgical Dupuytren biomarkers will permit disease staging from diagnostic and prognostic perspectives, as well as allows evaluation of biologic responses to treatment. Identification of such markers may transcend their use in Dupuytren treatment, because fibrotic biological processes fundamental to Dupuytren are relevant to fibrosis in many other connective tissues and organs with collagen-based tissue compartments. There is a wide range of potential Dupuytren biomarker categories that could be informative, including disease determinants linked to genetics, collagen metabolism, as well as immunity and inflammation (e.g., cytokines, chemokines). This narrative review provides a broad overview of previous studies","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"313 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A MicroRNA Approach to Evaluate Elevated Prostate Cancer Risk in Cancer-Free Men 评估未患癌症男性前列腺癌风险升高的 MicroRNA 方法

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-04-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053672

Scott Perrapato, Nicholas Farina, Adrian Berg, James Wallace, Steven Ades, Thomas Ahern, Janet Stein, Gary Stein, Jane Lian

引用次数: 0

PURPL promotes M2 macrophage polarization in lung cancer via regulating RBM4/xCT signaling PURPL 通过调节 RBM4/xCT 信号促进肺癌中 M2 巨噬细胞的极化

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-03-01 DOI: 10.1615/critreveukaryotgeneexpr.2024052788

Jipeng Guo, Chongwen Gong, Hao Wang

引用次数: 0