METTL16-Mediated Upregulation of FGD5-AS1 Promotes Progression of Osteosarcoma through Targeting of miR-195-5p/SLC7A2 Axis.

IF 1.6 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2025-01-01 DOI:10.1615/CritRevEukaryotGeneExpr.2025058118

Weiqian Wu, Wenbiao Zheng, Weiwei Pan, Fanghu Chen, Langqing Jiang

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引用次数: 0

Abstract

Dysregulated long noncoding RNAs (lncRNAs) promote the progression of osteosarcoma (OS). This study aimed to investigate the potential of lncRNA FGD5-AS1 in OS. Gene expression was determined using RT-qPCR. Protein expression was detected by western blot. N6-methyladenosine (m6A) modification was confirmed by MeRIP. Cell behaviors were detected using CCK-8, colony formation, and transwell assays. The interaction between miR-195-5p and the FGD5-AS1/SLC7A2 axis was confirmed by luciferase assays. FGD5-AS1 was upregulated in OS, induced by METTL16 via mediating m6A modification. However, FGD5-AS1 knockdown inhibited the proliferation and epithelial-mesenchymal transition (EMT) of OS cells. FGD5-AS1 sponged miR-195-5p to mediate the upregulation of SLC7A2, overexpression of which promoted aggressiveness of OS cells. In summary, METTL16-mediated upregulation of FGD5-AS1 promotes the aggressiveness of OS though targeting of miR-195-5p/SLC7A2 axis.

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mettl16介导的FGD5-AS1上调通过靶向miR-195-5p/SLC7A2轴促进骨肉瘤的进展。

失调的长链非编码rna （lncRNAs）促进骨肉瘤（OS）的进展。本研究旨在探讨lncRNA FGD5-AS1在OS中的潜在作用。RT-qPCR检测基因表达。western blot检测蛋白表达。通过MeRIP验证了n6 -甲基腺苷（m6A）的修饰。使用CCK-8、菌落形成和transwell检测细胞行为。通过荧光素酶测定证实了miR-195-5p与FGD5-AS1/SLC7A2轴之间的相互作用。METTL16通过介导m6A修饰诱导FGD5-AS1在OS中表达上调。然而，FGD5-AS1敲低抑制OS细胞的增殖和上皮-间质转化（EMT）。FGD5-AS1海绵miR-195-5p介导SLC7A2的上调，SLC7A2的过表达促进了OS细胞的侵袭性。综上所述，mettl16介导的FGD5-AS1上调通过靶向miR-195-5p/SLC7A2轴促进OS的侵袭性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.