Critical Reviews in Eukaryotic Gene Expression最新文献

Exosomal circ_001860 promotes colorectal cancer progression through miR-582-5p/ZEB1 axis 外泌体circ_001860通过miR-582-5p/ZEB1轴促进结直肠癌进展

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-09-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054404

Weizhen Huang, Jun Li, Siwei Zhou, Yi Li, Xia Yuan

{"title":"Exosomal circ_001860 promotes colorectal cancer progression through miR-582-5p/ZEB1 axis","authors":"Weizhen Huang, Jun Li, Siwei Zhou, Yi Li, Xia Yuan","doi":"10.1615/critreveukaryotgeneexpr.2024054404","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054404","url":null,"abstract":"Extensive research has recently been conducted to investigate the regulating impact of exosomal circular RNAs (circRNAs) in throughout the development of multiple malignancies. Nevertheless, there is still much to learn about the biological roles and underlying mechanisms of exosomal circRNAs in colorectal cancer (CRC). Exosomes (exo) were isolated from blood samples and CRC cells by differential centrifugation. In addition, the competitive endogenous RNA (ceRNA) mechanism of circ_001860 in CRC was determined through Starbase and dual-luciferase reporter gene experiments. Gain and loss of function experiments verified the regulatory effect of circ_001860/miR-582-5p/ZEB1 on the malignant phenotype of CRC cells. The therapeutic effect of circ_001860 on CRC xenograft tumor model was explored through mouse experiment. Circ_001860 was significantly enriched in exo isolated from CRC blood samples and CRC cells. Circ_001860 can be transported into CRC cells via exo. Through competitive binding to miR-582-5p, circ_001860 increased ZEB1, thereby facilitating tumor formation in vivo as well as stimulating CRC cell proliferation and metastasis in vitro. Through the miR-582-5p/ZEB1 axis, exosomal circ_001860 enhanced the advancement of CRC. This finding may offer non-invasive biomarkers for clinical screening and diagnosis of CRC patients.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"2 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosaminoglycans (GAGs) adenogenesis factors: immunohistochemical espression in endometriosis tissues compared to the endometrium 糖胺聚糖（GAGs）腺生成因子：与子宫内膜相比，在子宫内膜异位症组织中的免疫组化表达

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-09-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054273

Pietro G Signorile, Alfonso Baldi, Rosa Viceconte, Emma Carraturo, Maria Rosaria Boccellino, Mario Fordellone, Marco Montella

{"title":"Glycosaminoglycans (GAGs) adenogenesis factors: immunohistochemical espression in endometriosis tissues compared to the endometrium","authors":"Pietro G Signorile, Alfonso Baldi, Rosa Viceconte, Emma Carraturo, Maria Rosaria Boccellino, Mario Fordellone, Marco Montella","doi":"10.1615/critreveukaryotgeneexpr.2024054273","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054273","url":null,"abstract":"Endometriosis is a chronic inflammatory pathology estrogen-dependent. It is a condition affecting 5%–10% of women of reproductive age worldwide. Recent evidence indicating an embryological origin of endometriosis has provided new insights into its pathogenesis and potential therapeutic approaches. In this study, we compared the immunohistochemical expression of extracellular matrix molecules involved in the interaction between epithelium and stroma in endometriotic lesions and normal endometrial tissue.\u0000A total of 41 cases were analyzed. We examined the immunohistochemical expression of Chondroitin sulfate proteoglycan 4 (CSPG4), Keratan sulfate, Chondroitin sulfate (CS-56), Hyaluronic acid, and Heparan sulfate (HEP).\u0000Our results showed higher expression of CSPG4 and CS-56 in epithelial endometriosis samples compared to normal endometrial tissue, while HEP, Keratan sulfate, and Hyaluronic acid showed decreased expression in epithelial endometriosis samples relative to normal endometrial tissue. Additionally, endometriotic stroma exhibited more frequent low intensity of Hyaluronic acid and HEP compared to normal endometrial stroma.\u0000Investigating the levels of these molecules in eutopic and ectopic endometrial tissues enables the identification of potential therapeutic targets and the development of novel treatments aimed at disrupting the adhesive and invasive properties of endometriotic lesions.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"10 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin-carbon dots suppress periodontitis via regulating METTL3/IRE1α signaling 姜黄素碳点通过调节 METTL3/IRE1α 信号抑制牙周炎

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-08-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054463

Ling Li, Yueyan Wang, Lang Gao, Shunying Wu, Ying Jin

引用次数: 0

DNMT1-dependent DNA methylation of lncRNA FTX inhibits the ferroptosis of hepatocellular carcinoma lncRNA FTX的DNA甲基化依赖于DNMT1，可抑制肝细胞癌的铁变态反应

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-07-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054376

Sunfu Fan, Chaodan Shao, Shengnan Jia, Dafei Xie, Bingqi Yu

{"title":"DNMT1-dependent DNA methylation of lncRNA FTX inhibits the ferroptosis of hepatocellular carcinoma","authors":"Sunfu Fan, Chaodan Shao, Shengnan Jia, Dafei Xie, Bingqi Yu","doi":"10.1615/critreveukaryotgeneexpr.2024054376","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054376","url":null,"abstract":"Hepatocellular carcinoma (HCC) is one of the most malignant solid tumors worldwide. Long non-coding RNAs (lncRNAs) are the key factor in the pathogenesis of HCC. This study aimed to investigate the roles of lncRNA FTX in HCC. mRNA levels were detected using RT-qPCR. Protein expression was determined using western blot. cellular functions were determined using CCK-8 and PI staining assay. RNA fluorescent in situ hybridization (FISH) assay was conducted to analyze the location of lncRNA FTX and DNMT1. RNA pulldown, RNA immunoprecipitation (RIP), and chromatin-immunoprecipitation (ChIP) assays were used to ascertain the involved mechanisms. We found that FTX was downregulated in HCC patients, which was associated with poor prognosis. Moreover, DNMT1-mediated methylation of FTX promoter inhibited its expression. Interestingly, overexpression of FTX promoted the ferroptosis of HCC cells. FTX sponged miR-374b-3p to upregulate TFRC expression. However, downregulation of miR-374b-3p or overexpression of TFRC alleviated the effects of FTX knockdown and promoted the survival of HCC cells. In conclusion, DNMT1-dependent DNA methylation of FTX promotes the development of HCC through regulating miR-374b-3p/TFRC axis. Therefore, DNMT1/FTX/miR-374b-3p/TFRC axis may be a potential target for HCC.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"38 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141551615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATF4 exacerbates cerebral infarction-induced sensory dysfunction via HDAC1/DNMT1/GPX4 signaling ATF4 通过 HDAC1/DNMT1/GPX4 信号转导加剧脑梗塞诱发的感觉功能障碍

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-07-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054443

Bingtuan Lu, Ninghui Mu, Pu Li, Xindan Zhang, Lili Tao, Haiping Yin, Xiaoming Yin

引用次数: 0

A Review: The bioactivities and mechanisms of fungus extracts and compounds in colon cancer 综述：真菌提取物和化合物在结肠癌中的生物活性和机制

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-07-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054308

Xinze Liu, Kaijing Sun, Lin Feng, Xin Jin, Ying Sun, Wei Wu, Changbao Chen, Xilin Wan

{"title":"A Review: The bioactivities and mechanisms of fungus extracts and compounds in colon cancer","authors":"Xinze Liu, Kaijing Sun, Lin Feng, Xin Jin, Ying Sun, Wei Wu, Changbao Chen, Xilin Wan","doi":"10.1615/critreveukaryotgeneexpr.2024054308","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054308","url":null,"abstract":"Colon cancer is a malignant tumor caused by a malignant lesion of the colonic mucosal epithelium, with a high incidence in recent years. Fungi substances contain polysaccharides, terpenes, flavonoids and other chemical components, and the diversity of chemical components determines the strength of its biological activity. Studies have shown that the chemical components in fungi can be used as drugs to inhibit the growth of colon cancer. All available information about the bioactivities and mechanisms of fungus extracts and compounds in colon cancer was supplied by library database and electronic search (PubMed, ScienceDirect, CNKI, Web of Science, Google Scholar, etc.). Fungi reflect significant anti colon cancer effects through effects on colon cancer cell proliferation, cell cycle, apoptosis, tumor growth and protein expression. At present, most research focus on colon cancer cells and animal models. This review is on the purpose of the inhibition effects of chemical components in fungi on colon cancer at two levels in vivo and in vitro were reviewed. All the studies demonstrated significant improvements in colorectal cancer cells and animals after the fungi substances interventions. In this review, we give a complete overview of this subject, and summarizes recent research findings about molecular mechanisms on anti colorectal cancer.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"55 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MS4A3 promotes the chemosensitivity of lung cancer via THAP1/EGFR pathways MS4A3 通过 THAP1/EGFR 通路促进肺癌的化疗敏感性

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053662

Zhihui Duan

{"title":"MS4A3 promotes the chemosensitivity of lung cancer via THAP1/EGFR pathways","authors":"Zhihui Duan","doi":"10.1615/critreveukaryotgeneexpr.2024053662","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053662","url":null,"abstract":"MS4A3 functions as a tumor suppressor in multi-type cancer. However, the role of MS4A3 in lung cancer is still unknown. Therefore, this study aims to investigate the potential of MS4A3 in lung cancer. RT-qPCR is carried out to detect mRNA expression. CCK-8 and colony formation assay are conducted to determine cell proliferation. Tube formation assay is performed to determine angiogenesis. Flow cytometry is used to calculate apoptosis rates. JASPAR is used to determine the binding motif of THAP1. Luciferase and ChIP assay are conducted to verify that MS4A3 can interact with THAP1 to transcriptionally inactivate EGFR. MS4A3 is downregulated in non-small cell lung cancer (NSCLC) patients, which predicts poor clinical outcomes of NSCLC patients. Overexpressed MS4A3 enhances the chemosensitivity of NSCLC cells to Osimertinib. Moreover, MS4A3 suppresses the proliferation and angiogenesis and promotes the apoptosis of NSCLC cells. Moreover, MS4A3 upregulates apoptosis-related THAP1 to inactivate EGFR. However, THAP1 knockdown attenuates the effects of MS4A3 and promotes the malignant behavior of NSCLC cells. MS4A3 functions as an anti-tumor gene in NSCLC. MS4A3/THAP1/EGFR signaling enhances the chemosensitivity of lung cancer to EGFR tyrosine kinase inhibitor (TKI).","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"32 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141253651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FTO-mediated m6A modification of FTH1 inhibits ferroptosis of neurons in neonatal cerebral hypoxic ischaemia FTO 介导的 FTH1 m6A 修饰可抑制新生儿脑缺氧缺血时神经元的铁蜕变

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054011

Yanhong Chen, Jia Huang

{"title":"FTO-mediated m6A modification of FTH1 inhibits ferroptosis of neurons in neonatal cerebral hypoxic ischaemia","authors":"Yanhong Chen, Jia Huang","doi":"10.1615/critreveukaryotgeneexpr.2024054011","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054011","url":null,"abstract":"FTO is aberrantly expressed in brain disorders. However, the roles of FTO in neonatal hypoxic-ischemic brain injury (HIE) are still unclear. This study aims to investigate the potential of FTO in neonatal HIE. Oxygen-glucose deprivation (OGD) was used to establish HIE in vitro. mRNA levels were detected by RT-qPCR. Protein expression was detected by western blot. The levels of MDA, SOD, Fe2+ and GSH was detected by specific kit. m6A sites were analyzed using SRAMP and further verify by MeRIP assay. Cell proliferation was determined by CCK-8. Cell death was determined by PI staining. FTO was downregulated in patients with neonatal HIE and OGD-treated neurons. Moreover, FTO mRNA expression was decreased in ferroptosis inducer, especially FAC. However, overexpression of FTO inhibited the ferroptosis of neurons. Moreover, FTO-mediated m6A modification of FTH1 suppressed its mRNA expression and stability, inhibiting its protein expression. However, overexpression of FTH1 abrogated the effects of FTO and promoted the ferroptosis of neurons. In summary, FTO functions as a protective role in neonatal HIE via inhibiting FTH1 signaling. Thence, targeting may be a promising strategy for FTO neonatal HIE.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"24 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141501318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KDM6A promotes angiogenesis, migration, and invasion of pancreatic cancer via activating LAMP3 KDM6A 通过激活 LAMP3 促进胰腺癌的血管生成、迁移和侵袭

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054038

Liang Xu, Sunfu Fan, Dafei Xie, Yongqi Yu

引用次数: 0

Runx2 as a Prognostic Factor in Human Cancers 作为人类癌症预后因素的 Runx2

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054162

Jennifer Toner, Jonathan Gordon, Haley Greenyer, Peter Kaufman, Janet Stein, Gary Stein, Jane Lian

{"title":"Runx2 as a Prognostic Factor in Human Cancers","authors":"Jennifer Toner, Jonathan Gordon, Haley Greenyer, Peter Kaufman, Janet Stein, Gary Stein, Jane Lian","doi":"10.1615/critreveukaryotgeneexpr.2024054162","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054162","url":null,"abstract":"The RUNX2 transcription factor was discovered as an essential transcriptional regulator for commitment to osteoblast lineage cells and bone formation. Expression of Runx2 in other tissues, such as breast, prostate and lung, has been linked to oncogenesis, cancer progression and metastasis. In this study, we sought to determine the extent of Runx2 involvement in other tumors using a pan–cancer analysis strategy. We correlated RUNX2 expression and clinical-pathological parameters in human cancers by interrogating using publicly available multiparameter clinical data. Our analysis demonstrated that altered RUNX2 expression or function is associated with several cancer types from different tissues. We identified that 3 tumor types were associated with increased RUNX2 expression and decreased expression of RUNX2 is associated with 4 other cancer types. Our pan-cancer analysis for RUNX2 revealed numerous other discoveries for RUNX2 regulation of different cancers identified in each of the pan-cancer databases we used. Both up and down regulation of RUNX2 was observed during progression of specific types of cancers in promoting the distinct types of cancers.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"59 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141192647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0