Critical Reviews in Eukaryotic Gene Expression最新文献_第3页

Glycosaminoglycans (GAGs) adenogenesis factors: immunohistochemical espression in endometriosis tissues compared to the endometrium 糖胺聚糖（GAGs）腺生成因子：与子宫内膜相比，在子宫内膜异位症组织中的免疫组化表达

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-09-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054273

Pietro G Signorile, Alfonso Baldi, Rosa Viceconte, Emma Carraturo, Maria Rosaria Boccellino, Mario Fordellone, Marco Montella

{"title":"Glycosaminoglycans (GAGs) adenogenesis factors: immunohistochemical espression in endometriosis tissues compared to the endometrium","authors":"Pietro G Signorile, Alfonso Baldi, Rosa Viceconte, Emma Carraturo, Maria Rosaria Boccellino, Mario Fordellone, Marco Montella","doi":"10.1615/critreveukaryotgeneexpr.2024054273","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054273","url":null,"abstract":"Endometriosis is a chronic inflammatory pathology estrogen-dependent. It is a condition affecting 5%–10% of women of reproductive age worldwide. Recent evidence indicating an embryological origin of endometriosis has provided new insights into its pathogenesis and potential therapeutic approaches. In this study, we compared the immunohistochemical expression of extracellular matrix molecules involved in the interaction between epithelium and stroma in endometriotic lesions and normal endometrial tissue.\u0000A total of 41 cases were analyzed. We examined the immunohistochemical expression of Chondroitin sulfate proteoglycan 4 (CSPG4), Keratan sulfate, Chondroitin sulfate (CS-56), Hyaluronic acid, and Heparan sulfate (HEP).\u0000Our results showed higher expression of CSPG4 and CS-56 in epithelial endometriosis samples compared to normal endometrial tissue, while HEP, Keratan sulfate, and Hyaluronic acid showed decreased expression in epithelial endometriosis samples relative to normal endometrial tissue. Additionally, endometriotic stroma exhibited more frequent low intensity of Hyaluronic acid and HEP compared to normal endometrial stroma.\u0000Investigating the levels of these molecules in eutopic and ectopic endometrial tissues enables the identification of potential therapeutic targets and the development of novel treatments aimed at disrupting the adhesive and invasive properties of endometriotic lesions.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"10 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin-carbon dots suppress periodontitis via regulating METTL3/IRE1α signaling 姜黄素碳点通过调节 METTL3/IRE1α 信号抑制牙周炎

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-08-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054463

Ling Li, Yueyan Wang, Lang Gao, Shunying Wu, Ying Jin

引用次数: 0

DNMT1-dependent DNA methylation of lncRNA FTX inhibits the ferroptosis of hepatocellular carcinoma lncRNA FTX的DNA甲基化依赖于DNMT1，可抑制肝细胞癌的铁变态反应

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-07-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054376

Sunfu Fan, Chaodan Shao, Shengnan Jia, Dafei Xie, Bingqi Yu

{"title":"DNMT1-dependent DNA methylation of lncRNA FTX inhibits the ferroptosis of hepatocellular carcinoma","authors":"Sunfu Fan, Chaodan Shao, Shengnan Jia, Dafei Xie, Bingqi Yu","doi":"10.1615/critreveukaryotgeneexpr.2024054376","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054376","url":null,"abstract":"Hepatocellular carcinoma (HCC) is one of the most malignant solid tumors worldwide. Long non-coding RNAs (lncRNAs) are the key factor in the pathogenesis of HCC. This study aimed to investigate the roles of lncRNA FTX in HCC. mRNA levels were detected using RT-qPCR. Protein expression was determined using western blot. cellular functions were determined using CCK-8 and PI staining assay. RNA fluorescent in situ hybridization (FISH) assay was conducted to analyze the location of lncRNA FTX and DNMT1. RNA pulldown, RNA immunoprecipitation (RIP), and chromatin-immunoprecipitation (ChIP) assays were used to ascertain the involved mechanisms. We found that FTX was downregulated in HCC patients, which was associated with poor prognosis. Moreover, DNMT1-mediated methylation of FTX promoter inhibited its expression. Interestingly, overexpression of FTX promoted the ferroptosis of HCC cells. FTX sponged miR-374b-3p to upregulate TFRC expression. However, downregulation of miR-374b-3p or overexpression of TFRC alleviated the effects of FTX knockdown and promoted the survival of HCC cells. In conclusion, DNMT1-dependent DNA methylation of FTX promotes the development of HCC through regulating miR-374b-3p/TFRC axis. Therefore, DNMT1/FTX/miR-374b-3p/TFRC axis may be a potential target for HCC.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"38 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141551615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATF4 exacerbates cerebral infarction-induced sensory dysfunction via HDAC1/DNMT1/GPX4 signaling ATF4 通过 HDAC1/DNMT1/GPX4 信号转导加剧脑梗塞诱发的感觉功能障碍

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-07-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054443

Bingtuan Lu, Ninghui Mu, Pu Li, Xindan Zhang, Lili Tao, Haiping Yin, Xiaoming Yin

引用次数: 0

MS4A3 promotes the chemosensitivity of lung cancer via THAP1/EGFR pathways MS4A3 通过 THAP1/EGFR 通路促进肺癌的化疗敏感性

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053662

Zhihui Duan

{"title":"MS4A3 promotes the chemosensitivity of lung cancer via THAP1/EGFR pathways","authors":"Zhihui Duan","doi":"10.1615/critreveukaryotgeneexpr.2024053662","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053662","url":null,"abstract":"MS4A3 functions as a tumor suppressor in multi-type cancer. However, the role of MS4A3 in lung cancer is still unknown. Therefore, this study aims to investigate the potential of MS4A3 in lung cancer. RT-qPCR is carried out to detect mRNA expression. CCK-8 and colony formation assay are conducted to determine cell proliferation. Tube formation assay is performed to determine angiogenesis. Flow cytometry is used to calculate apoptosis rates. JASPAR is used to determine the binding motif of THAP1. Luciferase and ChIP assay are conducted to verify that MS4A3 can interact with THAP1 to transcriptionally inactivate EGFR. MS4A3 is downregulated in non-small cell lung cancer (NSCLC) patients, which predicts poor clinical outcomes of NSCLC patients. Overexpressed MS4A3 enhances the chemosensitivity of NSCLC cells to Osimertinib. Moreover, MS4A3 suppresses the proliferation and angiogenesis and promotes the apoptosis of NSCLC cells. Moreover, MS4A3 upregulates apoptosis-related THAP1 to inactivate EGFR. However, THAP1 knockdown attenuates the effects of MS4A3 and promotes the malignant behavior of NSCLC cells. MS4A3 functions as an anti-tumor gene in NSCLC. MS4A3/THAP1/EGFR signaling enhances the chemosensitivity of lung cancer to EGFR tyrosine kinase inhibitor (TKI).","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"32 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141253651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Runx2 as a Prognostic Factor in Human Cancers 作为人类癌症预后因素的 Runx2

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054162

Jennifer Toner, Jonathan Gordon, Haley Greenyer, Peter Kaufman, Janet Stein, Gary Stein, Jane Lian

{"title":"Runx2 as a Prognostic Factor in Human Cancers","authors":"Jennifer Toner, Jonathan Gordon, Haley Greenyer, Peter Kaufman, Janet Stein, Gary Stein, Jane Lian","doi":"10.1615/critreveukaryotgeneexpr.2024054162","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054162","url":null,"abstract":"The RUNX2 transcription factor was discovered as an essential transcriptional regulator for commitment to osteoblast lineage cells and bone formation. Expression of Runx2 in other tissues, such as breast, prostate and lung, has been linked to oncogenesis, cancer progression and metastasis. In this study, we sought to determine the extent of Runx2 involvement in other tumors using a pan–cancer analysis strategy. We correlated RUNX2 expression and clinical-pathological parameters in human cancers by interrogating using publicly available multiparameter clinical data. Our analysis demonstrated that altered RUNX2 expression or function is associated with several cancer types from different tissues. We identified that 3 tumor types were associated with increased RUNX2 expression and decreased expression of RUNX2 is associated with 4 other cancer types. Our pan-cancer analysis for RUNX2 revealed numerous other discoveries for RUNX2 regulation of different cancers identified in each of the pan-cancer databases we used. Both up and down regulation of RUNX2 was observed during progression of specific types of cancers in promoting the distinct types of cancers.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"59 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141192647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer cell type dependent modifications of metastatic parameters by SLIT2-ROBO1 and RHOA cAMP signaling in response to TGFB1 and FGF2 癌细胞类型依赖于 SLIT2-ROBO1 和 RHOA cAMP 信号对 TGFB1 和 FGF2 转移参数的改变

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-06-01 DOI: 10.1615/critreveukaryotgeneexpr.2024054055

Abdul Rauf Shakoori, Quratulain Amjad, Gary S. Stein, Andre van Wijnen, Abdul Rauf Shakoori

{"title":"Cancer cell type dependent modifications of metastatic parameters by SLIT2-ROBO1 and RHOA cAMP signaling in response to TGFB1 and FGF2","authors":"Abdul Rauf Shakoori, Quratulain Amjad, Gary S. Stein, Andre van Wijnen, Abdul Rauf Shakoori","doi":"10.1615/critreveukaryotgeneexpr.2024054055","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054055","url":null,"abstract":"The epithelial to mesenchymal transition (EMT) is a multistep process involving structural and functional alterations that are required for cancer metastasis, as well as loss of epithelial markers (e.g., E-cadherin/CDH1) and gain of mesenchymal markers (e.g., N-cadherin/CDH2, vimentin/VIM). Pathological events modify cell-cell interactions, cell-matrix adhesion and extra cellular matrix integrity leading to cell migration, evasion from the primary tumor and augmented invasiveness in the metastatic niche. This transformation is modulated by multiple paracrine factors (e.g., chemokines, growth factor), as well as SLIT2-ROBO1 signaling that collectively regulate expression of RHO GTPases (e.g., RHOA) and EMT marker genes. Yet, the roles of SLIT proteins in cancer remain enigmatic. In some cancer types, SLIT2 is anti-tumorigenic, while in other cancers it contributes towards the metastatic phenotype. Here we investigated the ambivalent metastatic activity of SLIT2 by analyzing how cAMP/RHOA signal transduction modulates SLIT-ROBO controlled metastatic parameters in response to the phosphodiesterase inhibitor IBMX (3-isobutyl-1-methylxanthine) and paracrine factors (TGF-β/TGFB1 and FGF2). Upon SLIT2 administration cell migration and proliferation increases in colon cancer cells and decreases in cervical cancer cells, while altering cell morphology and proliferation in both cancer types. These effects are reinforced by TGF-β/TGBF1 and FGF2, but attenuated by elevation of cAMP with IBMX, depending on the cancer cell type. Our data indicate that SLIT2 represents a potential biomarker for cancer diagnosis, prognosis, and therapy.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"15 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141551616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture alleviates Parkinson’s disease via promoting METTL9-catalyzed histidine methylation of NF-κB 电针通过促进 METTL9 催化的 NF-κB 组氨酸甲基化缓解帕金森病

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053243

Xiang Zhou, Liang Zhou, Jiayi Sun, Juan Zhang, Lei Sun

{"title":"Electroacupuncture alleviates Parkinson’s disease via promoting METTL9-catalyzed histidine methylation of NF-κB","authors":"Xiang Zhou, Liang Zhou, Jiayi Sun, Juan Zhang, Lei Sun","doi":"10.1615/critreveukaryotgeneexpr.2024053243","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053243","url":null,"abstract":"This study aimed to investigate the effects of electroacupuncture (EA) treatment on PD. MPTP administration was used establish PD mice model. The number of neurons was determined using TH staining. mRNA expression was detected using RT-qPCR. Protein expression was detected using western blot. Gene expression was determined using immunofluorescence and immunohistochemistry. The functions of neurons were determined using TUNEL and flow cytometry assay. The binding sites on NF-κB RELA in the promoter of NLRP3 was predicted by JASPAR and verified by luciferase and ChIP assays. EA treatment improved motor dysfunction in patients with PD. In vivo assays showed that MPTP administration induced the loss of neurons in mice, which was restored by EA treatment. Moreover, EA treatment attenuates motor deficits in MPTP-induced PD mice. EA treatment also inhibited the enrichment of pro-inflammatory cytokines and LDH, and suppressed neuronal pyroptosis. EA treatment increased the expression of METTL9. However, METTL9 deficiency dampened the effects of EA treatment and induced neuronal pyroptosis. Additionally, METTL9 promoted histidine methylation of NF-κB RELA, resulting the inhibition of epigenetic transcription of NLRP3. EA treatment restored neuronal function and improved motor dysfunction via promoting METTL9 histidine methylation of NF-κB/NLRP3 signaling.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"78 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141059103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and clinicopathological significance of extracellular matrix remodeling markers in esophageal squamous carcinoma 细胞外基质重塑标记物在食管鳞癌中的表达及其临床病理学意义

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053646

Zhiqin Fan, Fei Chen, Yingmin Liu, Xiaotong Huang, Siyue Tian, Yuqing Ma

{"title":"Expression and clinicopathological significance of extracellular matrix remodeling markers in esophageal squamous carcinoma","authors":"Zhiqin Fan, Fei Chen, Yingmin Liu, Xiaotong Huang, Siyue Tian, Yuqing Ma","doi":"10.1615/critreveukaryotgeneexpr.2024053646","DOIUrl":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024053646","url":null,"abstract":"Esophageal squamous cell carcinoma (ESCC) is a common malignancy of the gastrointestinal tract with a single therapeutic option and a lack of effective clinical therapeutic biomarkers.Extracellular matrix (ECM) remodelling plays a pro-carcinogenic role in a variety of malignancies, but its role in esophageal squamous carcinoma remains to be elucidated.In this study, we examined the expression levels of ECM remodelling markers in 71 pairs of esophageal squamous carcinoma tissues and normal tissues adjacent to the carcinoma using immunohistochemical staining, and analysed their relationship with clinicopathological features and prognosis.The results suggested that extracellular matrix remodelling markers (Integrin αV, Fibronectin, MMP9) were abnormally highly expressed in esophageal squamous carcinoma tissues.There was a statistically significant difference between the positive expression of ECM remodelling and the TNM stage of esophageal squamous carcinoma, and there was no statistically significant correlation with age, gender and carcinoembryonic antigen expression, differentiation degree, T stage and lymph node metastasis.Overall survival rate and overall survival time were significantly lower in patients with positive ECM remodelling expression, which was an independent risk factor for poor prognosis of esophageal squamous carcinoma.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"41 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140884522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The alteration of the relationship between cells and their context is the basis of a pathological condition 细胞与其环境之间关系的改变是病理状态的基础

IF 1.6 4区医学

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI: 10.1615/critreveukaryotgeneexpr.2024053711

Domenico RIBATTI

引用次数: 0