Photoacoustics最新文献_第8页

Laser ultrasonic detection for defects of LAM components based on variable time window intensity mapping with adaptive 2σ thresholds 基于自适应 2σ 阈值的可变时间窗强度映射的激光超声波检测 LAM 组件的缺陷

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-08-06 DOI: 10.1016/j.pacs.2024.100638

Zhuangzhuang Wan , Xue Bai , Jian Ma , Zhaowen Xu , Yaolu Liu

{"title":"Laser ultrasonic detection for defects of LAM components based on variable time window intensity mapping with adaptive 2σ thresholds","authors":"Zhuangzhuang Wan , Xue Bai , Jian Ma , Zhaowen Xu , Yaolu Liu","doi":"10.1016/j.pacs.2024.100638","DOIUrl":"10.1016/j.pacs.2024.100638","url":null,"abstract":"<div><p>Metallurgical defects in metal laser additive manufacturing (LAM) are inevitable due to complex non-equilibrium thermodynamics. A laser ultrasonic system was designed for detecting surface/near-surface defects in the layer-by-layer LAM process. An approach was proposed for ultrasonic imaging of defects based on variable time window intensity mapping with adaptive 2σ threshold denoising. The Gaussian mixture model hypothesis and expectation-maximization algorithm can automatically differentiate between components dominated by defects and background noises, thereby providing an adaptive threshold that accommodates detection environments and surface roughness levels. Results show that the ultrasonic wave reflection at defect boundaries diminishes far-field ultrasonic intensity upon pulsed laser irradiation on surface defects, enabling defect size and location characterization. This method is applicable to LAM samples with a significant surface roughness of up to 37.5 μm. It can detect superficial and near-surface defects down to 0.5 mm in diameter and depth, making it significant for online defect detection in additive manufacturing.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"39 ","pages":"Article 100638"},"PeriodicalIF":7.1,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000557/pdfft?md5=671577a6b460aa11d1232b4d147abbdc&pid=1-s2.0-S2213597924000557-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141952808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Open-closed single-tube on-beam tuning-fork-enhanced fiber-optic photoacoustic spectroscopy 开闭式单管光束调谐叉增强光纤光声光谱仪

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-08-05 DOI: 10.1016/j.pacs.2024.100639

Yufeng Pan , Lujun Fu , Jiangshan Zhang , Ping Lu

{"title":"Open-closed single-tube on-beam tuning-fork-enhanced fiber-optic photoacoustic spectroscopy","authors":"Yufeng Pan , Lujun Fu , Jiangshan Zhang , Ping Lu","doi":"10.1016/j.pacs.2024.100639","DOIUrl":"10.1016/j.pacs.2024.100639","url":null,"abstract":"<div><p>A proof-of-concept on-beam tuning-fork-enhanced photoacoustic sensor based on an open-closed single-tube acoustic-microresonator (AmR) was proposed and investigated for the first time, to the best of our knowledge. Due to the high acoustic amplification effect, the open-closed AmR improved the detection sensitivity by 54 times with respect to the bare tuning fork (TF). Compared to traditional dual-tube/single-tube on-beam spectrophone configuration, the developed approach significantly facilitates the laser beam alignment and reduces the sensor size and gas consumption. A 6.6 kHz low-frequency custom aluminum alloy TF was employed as the acoustic transducer to detect the photoacoustic signal. The vibration of TF was measured by a fiber-optic Fabry-Pérot (FP) interferometer (FPI). The modulation depth, tube length and laser power were experimentally optimized and evaluated in detail. An acetylene (C<sub>2</sub>H<sub>2</sub>) 1σ minimum detection limit (MDL) of 6.3 ppb was obtained with a high laser power of ∼ 500 mW, corresponding to a normalized noise equivalent absorption (NNEA) coefficient of 6.5 × 10<sup>−9</sup> cm<sup>−1</sup> W/Hz<sup>1/2</sup>. The compact spectrophone size and all-fiber measurement method can make the PAS-based sensor have great application prospects in dissolved gases detection in transformer oil, remote gas detection, space-limited gas detection, and etc.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"39 ","pages":"Article 100639"},"PeriodicalIF":7.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000569/pdfft?md5=6d4cffa5677e7afef3c3bb8325f235ac&pid=1-s2.0-S2213597924000569-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-infrared multispectral photoacoustic analysis of lipids and intraplaque hemorrhage in human carotid artery atherosclerosis 对人体颈动脉粥样硬化中的脂质和斑块内出血进行近红外多谱段光声分析

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-08-01 DOI: 10.1016/j.pacs.2024.100636

Jonas J.M. Riksen , Sowmiya Chandramoorthi , Antonius F.W. Van der Steen , Gijs Van Soest

{"title":"Near-infrared multispectral photoacoustic analysis of lipids and intraplaque hemorrhage in human carotid artery atherosclerosis","authors":"Jonas J.M. Riksen , Sowmiya Chandramoorthi , Antonius F.W. Van der Steen , Gijs Van Soest","doi":"10.1016/j.pacs.2024.100636","DOIUrl":"10.1016/j.pacs.2024.100636","url":null,"abstract":"<div><p>Spectral photoacoustic imaging in combination with unmixing techniques may be applied to retrieve information about high-risk features present in atherosclerotic plaques, possibly providing prognostic insights into future stroke events. We present the photoacoustic spectral contrast found in 12 systematically scanned advanced atherosclerotic plaques in the near-infrared wavelength range (850–1250 nm). The main absorbers are lipid, water, and hemoglobin, with the highest photoacoustic intensities at the lipid’s second overtone at 1190 and 1210 nm. Linear unmixing resulted in visualizing regions with high lipid and hemoglobin absorption, corresponding to the histological presence of lipid and intraplaque hemorrhage. A non-negative matrix factorization approach reveals differences in lipid spectral contrast, providing potential insights into the vulnerability of atherosclerotic plaque. These results provide a reference for future, more complex, <em>in vivo</em> photoacoustic imaging of carotid artery atherosclerosis, potentially contributing to assessing the risk of future events and treatment decision.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"38 ","pages":"Article 100636"},"PeriodicalIF":7.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000533/pdfft?md5=fa47a7c8373980dde43bfcf8ce653a90&pid=1-s2.0-S2213597924000533-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141945456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: “Extreme ultraviolet transient gratings: A tool for nanoscale photoacoustics” [Photoacoustics 29 (2023) 100453] 更正："极紫外瞬态光栅：纳米级光声学工具" [Photoacoustics 29 (2023) 100453] 更正

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-07-31 DOI: 10.1016/j.pacs.2024.100637

L. Foglia , R. Mincigrucci , A.A. Maznev , G. Baldi , F. Capotondi , F. Caporaletti , R. Comin , D. De Angelis , R.A. Duncan , D. Fainozzi , G. Kurdi , J. Li , A. Martinelli , C. Masciovecchio , G. Monaco , A. Milloch , K.A. Nelson , C.A. Occhialini , M. Pancaldi , E. Pedersoli , F. Bencivenga

引用次数: 0

Simultaneous measurement of methane, propane and isobutane using a compact mid-infrared photoacoustic spectrophone 利用紧凑型中红外光声光谱仪同时测量甲烷、丙烷和异丁烷

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-07-30 DOI: 10.1016/j.pacs.2024.100635

Huaiyu Mei , Gaoxuan Wang , Yinghe Xu , Haijie He , Jun Yao , Sailing He

{"title":"Simultaneous measurement of methane, propane and isobutane using a compact mid-infrared photoacoustic spectrophone","authors":"Huaiyu Mei , Gaoxuan Wang , Yinghe Xu , Haijie He , Jun Yao , Sailing He","doi":"10.1016/j.pacs.2024.100635","DOIUrl":"10.1016/j.pacs.2024.100635","url":null,"abstract":"<div><p>Hydrocarbon gas sensing is a challenging task using laser absorption spectroscopy due to the complex and broad structure of absorption lines. This application requires quick, accurate and highly sensitive detection of hydrocarbon gases concentrations. In this paper, a compact photoacoustic spectrophone was developed to simultaneously measure methane, propane and isobutane. This spectrophone uses wavelength modulation spectroscopy (WMS) with a single acoustic resonator and a single DFB laser emitting at 3368 nm, which greatly reduces the system complexity without using time-division multiplexing technology for multi-gas sensing. Due to the complex and broadband absorption of hydrocarbon gases, a novel signal processing method based on multilinear regression with Ridge regression (MLR-RG) is proposed to reduce the measurement error caused by the nonlinearity of spectra signal. For single gas measurement, the detection limits of methane, propane, and isobutane are determined to be 828 ppb, 419 ppb, and 619 ppb (SNR <span><math><mo>=</mo></math></span> 1, integration time <span><math><mo>=</mo></math></span> 20 s), respectively. For simultaneous multi-gas sensing in a gaseous mixture, the detection limits of propane and isobutane are determined to be 7 ppb, 68 ppb with an integration time of 860 s, 460 s, respectively. The measurement accuracy of propane and isobutane using MLR-RG is higher than that of ordinary least squares regression and partial least squares regression by 75% and 60%, respectively. The proposed algorithm based on MLR-RG provides a promising approach to process the broad overlapping absorption spectra for accurately retrieving hydrocarbon gases concentrations.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"39 ","pages":"Article 100635"},"PeriodicalIF":7.1,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000521/pdfft?md5=e951b6daa845250160412ddb3ac51cdd&pid=1-s2.0-S2213597924000521-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harmonic phase-sensitive detection for quartz-enhanced photoacoustic-thermoelastic spectroscopy 用于石英增强光声热弹光谱的谐波相位敏感检测技术

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-07-10 DOI: 10.1016/j.pacs.2024.100633

Mengpeng Hu , Dongqing Zhang , Hui Zhang , Yu Liu , Weibiao Wang , Qiang Wang

{"title":"Harmonic phase-sensitive detection for quartz-enhanced photoacoustic-thermoelastic spectroscopy","authors":"Mengpeng Hu , Dongqing Zhang , Hui Zhang , Yu Liu , Weibiao Wang , Qiang Wang","doi":"10.1016/j.pacs.2024.100633","DOIUrl":"10.1016/j.pacs.2024.100633","url":null,"abstract":"<div><p>Quartz tuning fork (QTF)-based techniques of photoacoustic spectroscopy and thermoelastic spectroscopy play a significant role in trace gas sensing due to unique high sensitivity and compactness. However, the stability of both techniques remains plagued by the inevitable and unpredictable laser power variation and demodulation phase variation. Herein, we investigate the phase change of a QTF when integrating both techniques for enhanced gas sensing. By demonstrating harmonic phase-sensitive methane detection as an example, we achieve stable gas measurement at varying laser power (2.4–9.4 mW) and varying demodulation phase (−90–90°). Besides, this method shows more tolerance to resonant frequency drift, contributing to a small signal fluctuation of ≤ 6.4 % over a wide modulation range (>10 times of the QTF bandwidth). The realization of harmonic-phase detection allows strengthening the stability of QTF-based sensors in a simple manner, especially when stable parameters, such as laser power, demodulation phase, even resonant frequency, cannot always be maintained.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"38 ","pages":"Article 100633"},"PeriodicalIF":7.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000508/pdfft?md5=ffde7522b28f0260504dcaef4f69cfaa&pid=1-s2.0-S2213597924000508-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-sensitivity trace gas detection based on differential Helmholtz photoacoustic cell with dense spot pattern 基于具有密集光斑模式的差分亥姆霍兹光声电池的高灵敏度痕量气体检测技术

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-07-09 DOI: 10.1016/j.pacs.2024.100634

Chu Zhang, Ying He, Shunda Qiao, Yahui Liu, Yufei Ma

{"title":"High-sensitivity trace gas detection based on differential Helmholtz photoacoustic cell with dense spot pattern","authors":"Chu Zhang, Ying He, Shunda Qiao, Yahui Liu, Yufei Ma","doi":"10.1016/j.pacs.2024.100634","DOIUrl":"https://doi.org/10.1016/j.pacs.2024.100634","url":null,"abstract":"<div><p>A high-sensitivity photoacoustic spectroscopy (PAS) sensor based on differential Helmholtz photoacoustic cell (DHPAC) with dense spot pattern is reported in this paper for the first time. A multi-pass cell based on two concave mirrors was designed to achieve a dense spot pattern, which realized 212 times excitation of incident laser. A finite element analysis was utilized to simulate the sound field distribution and frequency response of the designed DHPAC. An erbium-doped fiber amplifier (EDFA) was employed to amplify the output optical power of the laser to achieve strong excitation. In order to assess the designed sensor's performance, an acetylene (C<sub>2</sub>H<sub>2</sub>) detection system was established using a near infrared diode laser with a central wavelength 1530.3 nm. According to experimental results, the differential characteristics of DHPAC was verified. Compared to the sensor without dense spot pattern, the photoacoustic signal with dense spot pattern had a 44.73 times improvement. The minimum detection limit (MDL) of the designed C<sub>2</sub>H<sub>2</sub>-PAS sensor can be improved to 5 ppb when the average time of the sensor system is 200 s.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"38 ","pages":"Article 100634"},"PeriodicalIF":7.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221359792400051X/pdfft?md5=c472b4d3cf77e9506db1ab5606b3d76c&pid=1-s2.0-S221359792400051X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141596838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unsupervised deep learning enables real-time image registration of fast-scanning optical-resolution photoacoustic microscopy 无监督深度学习实现快速扫描光学分辨光声显微镜的实时图像配准

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-07-05 DOI: 10.1016/j.pacs.2024.100632

Xiaobin Hong , Furong Tang , Lidai Wang , Jiangbo Chen

{"title":"Unsupervised deep learning enables real-time image registration of fast-scanning optical-resolution photoacoustic microscopy","authors":"Xiaobin Hong , Furong Tang , Lidai Wang , Jiangbo Chen","doi":"10.1016/j.pacs.2024.100632","DOIUrl":"https://doi.org/10.1016/j.pacs.2024.100632","url":null,"abstract":"<div><p>A fast scanner of optical-resolution photoacoustic microscopy is inherently vulnerable to perturbation, leading to severe image distortion and significant misalignment among multiple 2D or 3D images. Restoration and registration of these images is critical for accurately quantifying dynamic information in long-term imaging. However, traditional registration algorithms face a great challenge in computational throughput. Here, we develop an unsupervised deep learning based registration network to achieve real-time image restoration and registration. This method can correct artifacts from B-scan distortion and remove misalignment among adjacent and repetitive images in real time. Compared with conventional intensity based registration algorithms, the throughput of the developed algorithm is improved by 50 times. After training, the new deep learning method performs better than conventional feature based image registration algorithms. The results show that the proposed method can accurately restore and register the images of fast-scanning photoacoustic microscopy in real time, offering a powerful tool to extract dynamic vascular structural and functional information.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"38 ","pages":"Article 100632"},"PeriodicalIF":7.1,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000491/pdfft?md5=e6516bd7a7efc746e98722f5b7f2867a&pid=1-s2.0-S2213597924000491-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141596839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probe fusion all-optic OCT-PAM dual-mode imaging system for biomedical imaging 用于生物医学成像的探针融合全光学 OCT-PAM 双模成像系统

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-07-01 DOI: 10.1016/j.pacs.2024.100631

Ning Ding , Huiwen Jiang , Ben Xiang , Yao Yu , Cheng Ji , Jian Liu , Yuqian Zhao , Jingmin Luan , Yanqiu Yang , Yi Wang , Zhenhe Ma

{"title":"Probe fusion all-optic OCT-PAM dual-mode imaging system for biomedical imaging","authors":"Ning Ding , Huiwen Jiang , Ben Xiang , Yao Yu , Cheng Ji , Jian Liu , Yuqian Zhao , Jingmin Luan , Yanqiu Yang , Yi Wang , Zhenhe Ma","doi":"10.1016/j.pacs.2024.100631","DOIUrl":"https://doi.org/10.1016/j.pacs.2024.100631","url":null,"abstract":"<div><p>We proposed a non-contact photoacoustic (PA) detection method using spectral domain optical coherence tomography (SDOCT). Two interference spectrums (A-lines) were acquired before and after the PA excitation with SDOCT. PA signal propagated within the sample causing the vibration. The vibration inner the sample introduced phase change between the acquired two A-lines. Thus, the PA signal can be detected by evaluating the difference in phase between the two A-lines. Based on the method, an OCT-PAM dual-mode imaging system was constructed. In the system, SDOCT served as the detection unit for PAM. Thus, the combination of the two imaging modalities was simplified. Another advantage of the system is that it realizes non-contact all-optic detection, which is attractive for biomedical imaging. Using the system, we imaged phantoms of carbon fibers, asparagus leaves and human hairs. Furthermore, the cortical vasculature of rat was imaged <em>in vivo</em> and the flow status was evaluated quantitatively.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"38 ","pages":"Article 100631"},"PeriodicalIF":7.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221359792400048X/pdfft?md5=a7e50712e087130120a606c8951882fd&pid=1-s2.0-S221359792400048X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141542317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive optoacoustic imaging of dermal microcirculatory revascularization in diet-induced obese mice undergoing exercise intervention 对接受运动干预的饮食诱导型肥胖小鼠真皮微循环血管再造进行无创光声成像

IF 7.1 1区医学

Photoacoustics Pub Date : 2024-06-30 DOI: 10.1016/j.pacs.2024.100628

Shan Huang , Hailong He , Robby Zachariah Tom , Sarah Glasl , Pia Anzenhofer , Andre C. Stiel , Susanna M. Hofmann , Vasilis Ntziachristos

{"title":"Non-invasive optoacoustic imaging of dermal microcirculatory revascularization in diet-induced obese mice undergoing exercise intervention","authors":"Shan Huang , Hailong He , Robby Zachariah Tom , Sarah Glasl , Pia Anzenhofer , Andre C. Stiel , Susanna M. Hofmann , Vasilis Ntziachristos","doi":"10.1016/j.pacs.2024.100628","DOIUrl":"https://doi.org/10.1016/j.pacs.2024.100628","url":null,"abstract":"<div><p>Microcirculatory dysfunction has been observed in the dermal white adipose tissue (dWAT) and subcutaneous white adipose tissue (scWAT) of obese humans and has been proposed as an early prediction marker for cardio-metabolic disease progression. <em>In-vivo</em> visualization and longitudinal monitoring of microvascular remodeling in these tissues remains challenging. We compare the performance of two optoacoustic imaging methods, i.e. multi-spectral optoacoustic tomography (MSOT) and raster-scanning optoacoustic mesoscopy (RSOM) in visualizing lipid and hemoglobin contrast in scWAT and dWAT in a mouse model of diet-induced obesity (DIO) undergoing voluntary wheel running intervention for 32 weeks. MSOT visualized lipid and hemoglobin contrast in murine fat depots in a quantitative manner even at early stages of DIO. We show for the first time to our knowledge that RSOM allows precise visualization of the dWAT microvasculature and provides quantitative readouts of skin layer thickness and vascular density in dWAT and dermis. Combination of MSOT and RSOM resolved exercise-induced morphological changes in microvasculature density, tissue oxygen saturation, lipid and blood volume content in dWAT and scWAT. The combination of MSOT and RSOM may allow precise monitoring of microcirculatory dysfunction and intervention response in dWAT and scWAT in a mouse model for DIO. Our findings have laid out the foundation for future clinical studies using optoacoustic-derived vascular readouts from adipose tissues as a biomarker for monitoring microcirculatory function in metabolic disease.</p></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"38 ","pages":"Article 100628"},"PeriodicalIF":7.1,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213597924000454/pdfft?md5=a6c16dd1f34badab58fd3a932b351336&pid=1-s2.0-S2213597924000454-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141542333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0