Photoacoustics最新文献

A joint photoacoustic imaging and broadband spectral analysis for early-stage intraoperative pathology assessment: A case study with colorectal cancer

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-03-04 DOI: 10.1016/j.pacs.2025.100712

Fan Yang , Zhengduo Yang , Zheng Zhu , Siwei Zhu , Wei Song , Yong Yang , Xiaocong Yuan

{"title":"A joint photoacoustic imaging and broadband spectral analysis for early-stage intraoperative pathology assessment: A case study with colorectal cancer","authors":"Fan Yang , Zhengduo Yang , Zheng Zhu , Siwei Zhu , Wei Song , Yong Yang , Xiaocong Yuan","doi":"10.1016/j.pacs.2025.100712","DOIUrl":"10.1016/j.pacs.2025.100712","url":null,"abstract":"<div><div>Accurate and rapid intraoperative diagnosis of micro-infiltration in early-stage tumors presents a formidable challenge for decades. Here, we propose a novel diagnostic approach, that combines Photoacoustic Morphological Imaging (PAMI) with an <em>in situ</em> broadband Photoacoustic Spectral Analysis (PASA), to implement intraoperative assessment of early-stage tumor while its high-frequencies between 50 and 150 MHz respond to various nuclei specifically. Our system, a broadband Ultraviolet Photoacoustic Microscopy (bUV-PAM), uniquely integrates ultraviolet laser-induced nucleus-specific photoacoustic excitation with broadband photoacoustic detection (up to 176 MHz at −6 dB) via an optical surface wave sensor. This approach facilitates the simultaneous acquisition of morphological and spectral information from unstained tissue sections, yielding a comprehensive dual-modality virtual slice within a single raster scan. Using human colorectal tissue samples, we applied the joint PAMI and <em>in situ</em> PASA approach across 6 case groups. Morphological features in PAMI showed a high concordance with Hematoxylin and Eosin (H&E) staining, whereas micro-infiltrative features were too indistinct to be identified in both PAMI and H&E images. In contrast, the PASA effectively distinguishes between micro-infiltrated and non-infiltrated tissues, a finding validated by subsequent Immunohistochemical (IHC) assessments. The preliminary results suggest that the joint approach holds potential to enhance intraoperative detection of micro-infiltration, thereby offering a promising avenue for accurate and rapid surgical margin assessment.</div></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"43 ","pages":"Article 100712"},"PeriodicalIF":7.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoacoustic oxygenation imaging to identify ischemia/hypoxia injury and necrosis of intestine after acute intussusception: A comparative study with CDFI/CEUS

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-03-01 DOI: 10.1016/j.pacs.2025.100706

Hualin Yan , Zehui Gou , Hong Wang , Xiaoxia Zhu , Juxian Liu , Wenwu Ling , Lin Huang , Yan Luo

{"title":"Photoacoustic oxygenation imaging to identify ischemia/hypoxia injury and necrosis of intestine after acute intussusception: A comparative study with CDFI/CEUS","authors":"Hualin Yan , Zehui Gou , Hong Wang , Xiaoxia Zhu , Juxian Liu , Wenwu Ling , Lin Huang , Yan Luo","doi":"10.1016/j.pacs.2025.100706","DOIUrl":"10.1016/j.pacs.2025.100706","url":null,"abstract":"<div><div>Acute intussusception is a pediatric abdominal emergency that requires immediate diagnosis and treatment. However, accurately identifying bowel necrosis non-invasively remains challenging with conventional sonography. In our study, we investigated the potential of photoacoustic imaging (PAI) as an innovative method for assessing ischemia/hypoxia injury and intestinal necrosis in cases of acute intussusception. Using PAI, we measured intestinal oxygen saturation (sO<sub>2</sub>) levels and total hemoglobin (HbT) in various models of acute intussusception at different time points. Additionally, we evaluated blood supply and ischemia/hypoxia injury using color Doppler flow imaging (CDFI) and contrast-enhanced ultrasound (CEUS). Based on histopathological results, intestinal sO₂ measured by PAI demonstrated optimal diagnostic performance for both ischemia/hypoxia injury and intestinal necrosis, with AUC values of 0.997 and 0.982, respectively, while CDFI and CEUS showed relatively high diagnostic performance for both ischemia/hypoxia injury and intestinal necrosis. In conclusion, PAI represents a promising, non-invasive imaging modality for assessing acute intussusception.</div></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"43 ","pages":"Article 100706"},"PeriodicalIF":7.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical synchronous signal demodulation-based quartz-enhanced photoacoustic spectroscopy for remote, multi-point methane detection in complex environments

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-27 DOI: 10.1016/j.pacs.2025.100708

Bo Sun , Tingting Wei , Mingjiang Zhang , Lijun Qiao , Zhe Ma , Angelo Sampaolo , Pietro Patimisco , Vincenzo Spagnolo , Hongpeng Wu , Lei Dong

{"title":"Optical synchronous signal demodulation-based quartz-enhanced photoacoustic spectroscopy for remote, multi-point methane detection in complex environments","authors":"Bo Sun , Tingting Wei , Mingjiang Zhang , Lijun Qiao , Zhe Ma , Angelo Sampaolo , Pietro Patimisco , Vincenzo Spagnolo , Hongpeng Wu , Lei Dong","doi":"10.1016/j.pacs.2025.100708","DOIUrl":"10.1016/j.pacs.2025.100708","url":null,"abstract":"<div><div>We present a novel optical synchronized signal demodulation (OSSD) method applied in quartz-enhanced photoacoustic spectroscopy (QEPAS) for remote gas sensing. Using 1 % of the laser source as an optical synchronization signal, kilometer-scale remote gas detection was achieved, overcoming the challenges of long-distance real-time detection in complex environments with conventional QEPAS. A time-sharing OSSD-QEPAS system for sewer methane detection was subsequently developed. The system’s modulation depth was optimized, and the catalytic effect of water vapor on photoacoustic signals was validated, resulting in a CH₄ sensor achieving a detection limit of 445 ppb with a 300-ms averaging time, and an excellent linear dynamic range with a R<sup>2</sup> = 0.999. To demonstrate the stability, robustness, and accuracy of the OSSD-QEPAS system, continuous methane measurements covering a 14-hour period at two different sewer locations on campus were performed.</div></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"43 ","pages":"Article 100708"},"PeriodicalIF":7.1,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitivity improvement of quartz-enhanced photoacoustic spectroscopy using the stochastic resonance method

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-27 DOI: 10.1016/j.pacs.2025.100707

Yingchao Xie , Hao Xiong , Shiling Feng , Ning Pan , Chuan Li , Yixuan Liu , Ye Zhang , Ligang Shao , Gaopeng Lu , Kun Liu , Guishi Wang

引用次数: 0

Fast aberration correction in 3D transcranial photoacoustic computed tomography via a learning-based image reconstruction method

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-20 DOI: 10.1016/j.pacs.2025.100698

Hsuan-Kai Huang , Joseph Kuo , Yang Zhang , Yousuf Aborahama , Manxiu Cui , Karteekeya Sastry , Seonyeong Park , Umberto Villa , Lihong V. Wang , Mark A. Anastasio

{"title":"Fast aberration correction in 3D transcranial photoacoustic computed tomography via a learning-based image reconstruction method","authors":"Hsuan-Kai Huang , Joseph Kuo , Yang Zhang , Yousuf Aborahama , Manxiu Cui , Karteekeya Sastry , Seonyeong Park , Umberto Villa , Lihong V. Wang , Mark A. Anastasio","doi":"10.1016/j.pacs.2025.100698","DOIUrl":"10.1016/j.pacs.2025.100698","url":null,"abstract":"<div><div>Transcranial photoacoustic computed tomography (PACT) holds significant potential as a neuroimaging modality. However, compensating for skull-induced aberrations in reconstructed images remains a challenge. Although optimization-based image reconstruction methods (OBRMs) can account for the relevant wave physics, they are computationally demanding and generally require accurate estimates of the skull’s viscoelastic parameters. To circumvent these issues, a learning-based image reconstruction method was investigated for three-dimensional (3D) transcranial PACT. The method was systematically assessed in virtual imaging studies that involved stochastic 3D numerical head phantoms and applied to experimental data acquired by use of a physical head phantom that involved a human skull. The results demonstrated that the learning-based method yielded accurate images and exhibited robustness to errors in the assumed skull properties, while substantially reducing computational times compared to an OBRM. To the best of our knowledge, this is the first demonstration of a learned image reconstruction method for 3D transcranial PACT.</div></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"43 ","pages":"Article 100698"},"PeriodicalIF":7.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

All-fiber three-wavelength laser for functional photoacoustic microscopy

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-20 DOI: 10.1016/j.pacs.2025.100703

Mingxuan Yang , Zheng Qu , Mohammadreza Amjadian , Xu Tang , Jiangbo Chen , Lidai Wang

{"title":"All-fiber three-wavelength laser for functional photoacoustic microscopy","authors":"Mingxuan Yang , Zheng Qu , Mohammadreza Amjadian , Xu Tang , Jiangbo Chen , Lidai Wang","doi":"10.1016/j.pacs.2025.100703","DOIUrl":"10.1016/j.pacs.2025.100703","url":null,"abstract":"<div><div>Advanced multi-wavelength pulsed laser is a key technique for functional optical-resolution photoacoustic microscopy (OR-PAM). By utilizing the stimulated Raman scattering (SRS) effect, we can generate various wavelengths from a single-wavelength pump laser, offering a simple and cost-effective solution for OR-PAM. However, existing multi-wavelength SRS lasers typically require fine alignment of many free-space optical components with single-mode fibers, which are susceptible to mechanical disturbances and temperature fluctuations, leading to high maintenance costs. To address this challenge, we develop an all-fiber three-wavelength SRS laser source for functional OR-PAM. A pump laser beam is launched into an optical fiber network, which splits and delays these laser pulses and generates different optical wavelengths in different fiber branches, and then merges them at the output end of the fiber network. This approach requires only one instance of fiber launching, dramatically simplifying the alignment and improving the laser stability. Using a decoding algorithm, we can separate the PA signals from different optical wavelengths and then calculate oxygen saturation (sO<sub>2</sub>) and flow speed. The SRS fiber network provides stable energy ratios among different optical wavelengths during long-time operation. We use the all-fiber OR-PAM system to monitor the brain function for four hours, demonstrating exceptional stability in functional imaging. The small size, simple structure, and low cost make it suitable for many preclinical and clinical applications.</div></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"42 ","pages":"Article 100703"},"PeriodicalIF":7.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sulfur dioxide detection platform based on photoacoustic spectroscopy and a 266.22 nm high-power stabilized LD-pumped solid-state Q-switched laser

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-18 DOI: 10.1016/j.pacs.2025.100702

Xiaowen Shen , Yixin Zhang , Ruyue Cui , Donghe Tian , Ming Cheng , Pietro Patimisco , Angelo Sampaolo , Chaofeng Sun , Xukun Yin , Vincenzo Spagnolo , Lei Dong , Hongpeng Wu

引用次数: 0

Hybrid transformer-CNN network-driven optical-scanning undersampling for photoacoustic remote sensing microscopy

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-17 DOI: 10.1016/j.pacs.2025.100697

Yihan Pi , Jijing Chen , Kaixuan Ding , Tongyan Zhang , Hao Zhang , Bingxue Zhang , Junhao Guo , Zhen Tian , Jiao Li

{"title":"Hybrid transformer-CNN network-driven optical-scanning undersampling for photoacoustic remote sensing microscopy","authors":"Yihan Pi , Jijing Chen , Kaixuan Ding , Tongyan Zhang , Hao Zhang , Bingxue Zhang , Junhao Guo , Zhen Tian , Jiao Li","doi":"10.1016/j.pacs.2025.100697","DOIUrl":"10.1016/j.pacs.2025.100697","url":null,"abstract":"<div><div>Imaging speed is critical for photoacoustic microscopy as it affects the capability to capture dynamic biological processes and support real-time clinical applications. Conventional approaches for increasing imaging speed typically involve high-repetition-rate lasers, which pose a risk of thermal damage to samples. Here, we propose a deep-learning-driven optical-scanning undersampling method for photoacoustic remote sensing (PARS) microscopy, accelerating imaging acquisition while maintaining a constant laser repetition rate and reducing laser dosage. We develop a hybrid Transformer-Convolutional Neural Network, HTC-GAN, to address the challenges of both nonuniform sampling and motion misalignment inherent in optical-scanning undersampling. A mouse ear vasculature image dataset is created through our customized galvanometer-scanned PARS system to train and validate HTC-GAN. The network successfully restores high-quality images from 1/2-undersampled and 1/4-undersampled data, closely approximating the ground truth images. A series of performance experiments demonstrate that HTC-GAN surpasses the basic misalignment compensation algorithm, and standalone CNN or Transformer networks in terms of perceptual quality and quantitative metrics. Moreover, three-dimensional imaging results validate the robustness and versatility of the proposed optical-scanning undersampling imaging method across multiscale scanning modes. Our method achieves a fourfold improvement in PARS imaging speed without hardware upgrades, offering an available solution for enhancing imaging speed in other optical-scanning microscopic systems.</div></div>","PeriodicalId":56025,"journal":{"name":"Photoacoustics","volume":"42 ","pages":"Article 100697"},"PeriodicalIF":7.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive H2S-LITES sensor with 80 m fiber-coupled multi-pass cell based on optical path multiplexing technology

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-11 DOI: 10.1016/j.pacs.2025.100699

Haiyue Sun , Ying He , Shunda Qiao , Chu Zhang , Yufei Ma

引用次数: 0

Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy

IF 7.1 1区医学

Photoacoustics Pub Date : 2025-02-10 DOI: 10.1016/j.pacs.2025.100696

Taoran Le, Jiarui Li, Haoyun Wei, Yan Li

引用次数: 0