Biofilm最新文献

{"title":"Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy","authors":"Tom Coenye ,&nbsp;Merja Ahonen ,&nbsp;Skip Anderson ,&nbsp;Miguel Cámara ,&nbsp;Parvathi Chundi ,&nbsp;Matthew Fields ,&nbsp;Ines Foidl ,&nbsp;Etienne Z. Gnimpieba ,&nbsp;Kristen Griffin ,&nbsp;Jamie Hinks ,&nbsp;Anup R. Loka ,&nbsp;Carol Lushbough ,&nbsp;Cait MacPhee ,&nbsp;Natasha Nater ,&nbsp;Rasmita Raval ,&nbsp;Jo Slater-Jefferies ,&nbsp;Pauline Teo ,&nbsp;Sandra Wilks ,&nbsp;Maria Yung ,&nbsp;Biofilm Priority Questions Exercise Participants ,&nbsp;Jeremy S. Webb","doi":"10.1016/j.bioflm.2024.100210","DOIUrl":"10.1016/j.bioflm.2024.100210","url":null,"abstract":"<div><p>Priority question exercises are increasingly used to frame and set future research, innovation and development agendas. They can provide an important bridge between the discoveries, data and outputs generated by researchers, and the information required by policy makers and funders. Microbial biofilms present huge scientific, societal and economic opportunities and challenges. In order to identify key priorities that will help to advance the field, here we review questions from a pool submitted by the international biofilm research community and from practitioners working across industry, the environment and medicine. To avoid bias we used computational approaches to group questions and manage a voting and selection process. The outcome of the exercise is a set of 78 unique questions, categorized in six themes: (i) Biofilm control, disruption, prevention, management, treatment (13 questions); (ii) Resistance, persistence, tolerance, role of aggregation, immune interaction, relevance to infection (10 questions); (iii) Model systems, standards, regulatory, policy education, interdisciplinary approaches (15 questions); (iv) Polymicrobial, interactions, ecology, microbiome, phage (13 questions); (v) Clinical focus, chronic infection, detection, diagnostics (13 questions); and (vi) Matrix, lipids, capsule, metabolism, development, physiology, ecology, evolution environment, microbiome, community engineering (14 questions). The questions presented are intended to highlight opportunities, stimulate discussion and provide focus for researchers, funders and policy makers, informing future research, innovation and development strategy for biofilms and microbial communities.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"8 ","pages":"Article 100210"},"PeriodicalIF":5.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000352/pdfft?md5=883e17aa6557cf972a1d0c614c8741b2&pid=1-s2.0-S2590207524000352-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141712422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yielding behaviour of chemically treated Pseudomonas fluorescens biofilms","authors":"Samuel G.V. Charlton ,&nbsp;Saikat Jana ,&nbsp;Jinju Chen","doi":"10.1016/j.bioflm.2024.100209","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100209","url":null,"abstract":"<div><p>The mechanics of biofilms are intrinsically shaped by their physicochemical environment. By understanding the influence of the extracellular matrix composition, pH and elevated levels of cationic species on the biofilm rheology, novel living materials with tuned properties can be formulated. In this study, we examine the role of a chaotropic agent (urea), two divalent cations and distilled deionized water on the nonlinear viscoelasticity of a model biofilm <em>Pseudomonas fluorescens</em>. The structural breakdown of each biofilm is quantified using tools of non-linear rheology. Our findings reveal that urea induced a softening response, and displayed strain overshoots comparable to distilled deionized water, without altering the microstructural packing fraction and macroscale morphology. The absorption of divalent ferrous and calcium cations into the biofilm matrix resulted in stiffening and a reduction in normalized elastic energy dissipation, accompanied by macroscale morphological wrinkling and moderate increases in the packing fraction. Notably, ferrous ions induced a predominance of rate dependent yielding, whereas the calcium ions resulted in equal contribution from both rate and strain dependent yielding and structural breakdown of the biofilms. Together, these results indicate that strain rate increasingly becomes an important factor controlling biofilm fluidity with cation-induced biofilm stiffening. The finding can help inform effective biofilm removal protocols and in development of bio-inks for additive manufacturing of biofilm derived materials.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"8 ","pages":"Article 100209"},"PeriodicalIF":5.9,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000340/pdfft?md5=e0fa5ba7428e1a9a6cb181f197016457&pid=1-s2.0-S2590207524000340-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices","authors":"Christian Kranjec ,&nbsp;Jills Puthiaparambil Mathew ,&nbsp;Kirill Ovchinnikov ,&nbsp;Idowu Fadayomi ,&nbsp;Ying Yang ,&nbsp;Morten Kjos ,&nbsp;Wen-Wu Li","doi":"10.1016/j.bioflm.2024.100211","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100211","url":null,"abstract":"<div><p>The ever-increasing use of exogenous materials as indwelling medical devices in modern medicine offers to pathogens new ways to gain access to human body and begin, in some cases, life threatening infections. Biofouling of such materials with bacteria or fungi is a major concern during surgeries, since this is often associated with biofilm formation and difficult to treat, recalcitrant infections. Intense research efforts have therefore developed several strategies to shield the medical devices' surface from colonization by pathogenic microorganisms. Here, we used dopamine as a coupling agent to coat four different materials of medical interest (plastic polyetheretherketone (PEEK), stainless steel, titanium and silicone catheter) with the bacteriocins, enterocin EJ97-short and the thiopeptide micrococcin P1. Water contact angle measurements and x-ray photoelectron spectroscopy were used to verify the effective coating of the materials. The effect of bacteriocins coated on these materials on the biofilm formation by a vancomycin resistant <em>Enterococcus faecium</em> (VRE) strain was studied by biofilm-oriented antimicrobial test (BOAT) and electron scanning microscopy. The <em>in vitro</em> biocompatibility of bacteriocin-modified biomaterials was tested on cultured human cells. The results demonstrated that the binding of the bacteriocins to the implant surfaces is achieved, and the two bacteriocins in combination could inhibit biofilm formation by <em>E. faecium</em> on all four materials. The modified implant showed no cytotoxicity to the human cells tested. Therefore, surface modification with the two bacteriocins may offer a novel and effective way to prevent biofilm formation on a wide range of implant materials.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"8 ","pages":"Article 100211"},"PeriodicalIF":5.9,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000364/pdfft?md5=6a812a9466c80e7d2f49afa80a40ef5d&pid=1-s2.0-S2590207524000364-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dopamine, an exogenous quorum sensing signaling molecule or a modulating factor in Pseudomonas aeruginosa?","authors":"Shi-Liang Xiang ,&nbsp;Kai-Zhong Xu ,&nbsp;Lu-Jun Yin ,&nbsp;Yong Rao ,&nbsp;Bo Wang ,&nbsp;Ai-Qun Jia","doi":"10.1016/j.bioflm.2024.100208","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100208","url":null,"abstract":"<div><p><em>Pseudomonas aeruginosa</em> is recognized globally as an opportunistic pathogen of considerable concern due to its high virulence and pathogenicity, especially in immunocompromised individuals. While research has identified several endogenous quorum sensing (QS) signaling molecules that enhance the virulence and pathogenicity of <em>P. aeruginosa</em>, investigations on exogenous QS signaling molecules or modulating factors remain limited. This study found that dopamine serves as an exogenous QS signaling molecule or modulating factor of <em>P. aeruginosa</em> PAO1, enhancing the production of virulence factors and biofilms. Compared to the control group, treatment with 40 μM dopamine resulted in a 33.1 % increase in biofilm formation, 68.1 % increase in swimming mobility, 63.1 % increase in swarming mobility, 147.2 % increase in the signaling molecule 3-oxo-C12-HSL, and 50.5 %, 28.5 %, 27.0 %, and 33.2 % increases in the virulence factors alginate, rhamnolipids, protease, and pyocyanin, respectively. This study further explored the mechanism of dopamine regulating the biofilm formation and virulence of <em>P. aeruginosa</em> PAO1 through transcriptome and metabolome. Transcriptomic analysis showed that dopamine promoted the expression of virulence genes <em>psl, alg, lasA, rhlABC</em>, <em>rml</em>, and <em>phz</em> in <em>P. aeruginosa</em> PAO1. Metabolomic analysis revealed changes in the concentrations of tryptophan, pyruvate, ethanolamine, glycine, 3-hydroxybutyric acid, and alizarin. Furthermore, KEGG enrichment analysis of altered genes and metabolites indicated that dopamine enhanced phenylalanine, tyrosine, and tryptophan in <em>P. aeruginosa</em> PAO1. The results of this study will contribute to the development of novel exogenous QS signaling molecules or modulating factors and advance our understanding of the interactions between <em>P. aeruginosa</em> and the host environment.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"8 ","pages":"Article 100208"},"PeriodicalIF":5.9,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000339/pdfft?md5=33d4e083969c17e1f4fdc34a2d89b8ea&pid=1-s2.0-S2590207524000339-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dynamics of biofilm development and dispersal should be taken into account when quantifying biofilm via the crystal violet microtiter plate assay","authors":"Jens Bo Andersen,&nbsp;Morten Rybtke,&nbsp;Tim Tolker-Nielsen","doi":"10.1016/j.bioflm.2024.100207","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100207","url":null,"abstract":"<div><p>The crystal violet microtiter plate biofilm assay is often used to compare the amount of biofilm formed by a mutant versus wild-type or a compound-treated biofilm versus the non-treatment control. In many of these studies the amount of biofilm is assessed only at one single time point. However, if the dynamics of biofilm development of the mutant (or compound-treated biofilm) is different than that of the wild-type (or non-treatment control), then biofilm quantification at a single time point may give misleading results. To overcome this shortcoming of the common biofilm quantification technique, we recommend to use a serial dilution-based crystal violet microtiter plate biofilm assay for easy assessment of the dynamics of biofilm development and dispersal. We demonstrate that the dilution-resolved crystal violet assay displays the dynamics of <em>Pseudomonas aeruginosa</em> biofilm development and dispersal as efficient as a time-resolved crystal violet assay. In addition, focusing on mutants of different parts of the c-di-GMP signaling system in <em>P. aeruginosa</em>, we provide an example illustrating the need to assess biofilm dynamics instead of quantifying biofilm biomass at a single time point.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"8 ","pages":"Article 100207"},"PeriodicalIF":5.9,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000327/pdfft?md5=21e154782abc4bbe0cdc803a1b46ecec&pid=1-s2.0-S2590207524000327-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141438432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myrtus communis leaf compounds as novel inhibitors of quorum sensing-regulated virulence factors and biofilm formation: In vitro and in silico investigations","authors":"Nadine Khadraoui ,&nbsp;Rym Essid ,&nbsp;Bilel Damergi ,&nbsp;Nadia Fares ,&nbsp;Dorra Gharbi ,&nbsp;Abel Mateo Forero ,&nbsp;Jaime Rodríguez ,&nbsp;Ghassen Abid ,&nbsp;Erika-Beáta Kerekes ,&nbsp;Ferid Limam ,&nbsp;Carlos Jiménez ,&nbsp;Olfa Tabbene","doi":"10.1016/j.bioflm.2024.100205","DOIUrl":"10.1016/j.bioflm.2024.100205","url":null,"abstract":"<div><p>Antibiotic resistance of the Gram-negative bacterium <em>Pseudomonas aeruginosa</em> and its ability to form biofilm through the Quorum Sensing (QS) mechanism are important challenges in the control of infections caused by this pathogen. The extract of <em>Myrtus communis</em> (myrtle) showed strong anti-QS effect on <em>C</em><em>hromobacterium</em><em>. violaceum</em> 6267 by inhibiting 80 % of the production of violacein pigment at a sub-MIC concentration of 1/8 (31.25 μg/mL). In addition, the extract exhibited an inhibitory effect on virulence factors of <em>P. aeruginosa</em> PAO1 at half MIC (125 μg/mL), significantly reducing the formation of biofilms (72.02 %), the swarming activity (75 %), and the production of protease (61.83 %) and pyocyanin (97 %). The active fraction also downregulated the expression of selected regulatory genes involved in the biofilm formation and QS in the <em>P. aeruginosa</em> PAO1 strain. These genes included the autoinducer synthase genes (<em>lasI</em> and <em>rhlI</em>), the genes involved in the expression of their corresponding receptors (<em>lasR</em> and <em>rhlR</em>), and the <em>pqsA</em> genes. The analysis of the active fraction by HPLC/UV/MS and NMR allowed the identification of three phenolic compounds, 3,5-di-<em>O</em>-galloylquinic acid, myricetin 3-<em>O</em>-α-<span>l</span>-rhamnopyranoside (myricitrin), and myricetin 3-<em>O</em>-(2″-<em>O</em>-galloyl)-ß-<span>d</span>-galactopyranoside. <em>In silico</em> studies showed that 3,5-di-<em>O</em>-galloylquinic acid, with an affinity score of −9.20 kcal/mol, had the highest affinity to the active site of the CviR protein (3QP8), a QS receptor from <em>C. violaceum</em>. Additionally, myricetin 3-<em>O</em>-α-<span>l</span>-rhamnopyranoside (myricitrin) and myricetin 3-<em>O</em>-(2″-<em>O</em>-galloyl)-ß-<span>d</span>-galactopyranoside interact to a lesser extent with 3QP8. In conclusion, this study contributed significantly to the discovery of new QS inhibitors from <em>M. communis</em> leaves against resistant Gram-negative pathogens.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"8 ","pages":"Article 100205"},"PeriodicalIF":6.8,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000303/pdfft?md5=e1ab8e0c2e42512f1b9627761e5bcdba&pid=1-s2.0-S2590207524000303-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141412258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eurobiofilms 2022: A translational perspective of biofilm-related persistent infections","authors":"María D. Macià,&nbsp;Elisa Borghi,&nbsp;Antonio Oliver","doi":"10.1016/j.bioflm.2023.100168","DOIUrl":"10.1016/j.bioflm.2023.100168","url":null,"abstract":"","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100168"},"PeriodicalIF":6.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000655/pdfft?md5=7c65f715af2f40f263698f89b5c88b30&pid=1-s2.0-S2590207523000655-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139293972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the flow behavior around marine biofilms","authors":"Maria J. Romeu ,&nbsp;João M. Miranda ,&nbsp;Ed. D. de Jong ,&nbsp;João Morais ,&nbsp;Vítor Vasconcelos ,&nbsp;Jelmer Sjollema ,&nbsp;Filipe J. Mergulhão","doi":"10.1016/j.bioflm.2024.100204","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100204","url":null,"abstract":"<div><p><em>In vitro</em> platforms capable of mimicking the hydrodynamic conditions prevailing in natural aquatic environments have been previously validated and used to predict the fouling behavior on different surfaces. Computational Fluid Dynamics (CFD) has been used to predict the shear forces occurring in these platforms. In general, these predictions are made for the initial stages of biofilm formation, where the amount of biofilm does not affect the flow behavior, enabling the estimation of the shear forces that initial adhering organisms have to withstand. In this work, we go a step further in understanding the flow behavior when a mature biofilm is present in such platforms to better understand the shear rate distribution affecting marine biofilms. Using 3D images obtained by Optical Coherence Tomography, a mesh was produced and used in CFD simulations. Biofilms of two different marine cyanobacteria were developed in agitated microtiter plates incubated at two different shaking frequencies for 7 weeks. The biofilm-flow interactions were characterized in terms of the velocity field and shear rate distribution. Results show that global hydrodynamics imposed by the different shaking frequencies affect biofilm architecture and also that this architecture affects local hydrodynamics, causing a large heterogeneity in the shear rate field. Biofilm cells located in the streamers of the biofilm are subjected to much higher shear values than those located on the bottom of the streamers and this dispersion in shear rate values increases at lower bulk fluid velocities. This heterogeneity in the shear force field may be a contributing factor for the heterogeneous behavior in metabolic activity, growth status, gene expression pattern, and antibiotic resistance often associated with nutrient availability within the biofilm.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100204"},"PeriodicalIF":6.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000297/pdfft?md5=527afe352f84e94acfe7dfffc337596a&pid=1-s2.0-S2590207524000297-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiofilm activity of Prevotella species from the cystic fibrosis lung microbiota against Pseudomonas aeruginosa","authors":"Lucia Grassi ,&nbsp;Kyle L. Asfahl ,&nbsp;Sara Van den Bossche ,&nbsp;Ine Maenhout ,&nbsp;Andrea Sass ,&nbsp;Yannick Vande Weygaerde ,&nbsp;Eva Van Braeckel ,&nbsp;Bruno Verhasselt ,&nbsp;Jerina Boelens ,&nbsp;Michael M. Tunney ,&nbsp;Ajai A. Dandekar ,&nbsp;Tom Coenye ,&nbsp;Aurélie Crabbé","doi":"10.1016/j.bioflm.2024.100206","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100206","url":null,"abstract":"<div><p>It is increasingly recognized that interspecies interactions may modulate the pathogenicity of <em>Pseudomonas aeruginosa</em> during chronic lung infections. Nevertheless, while the interaction between <em>P. aeruginosa</em> and pathogenic microorganisms co-infecting the lungs has been widely investigated, little is known about the influence of other members of the lung microbiota on the infection process. In this study, we focused on investigating the impact of <em>Prevotella</em> species isolated from the sputum of people with cystic fibrosis (pwCF) on biofilm formation and virulence factor production by <em>P. aeruginosa</em>. Screening of a representative collection of <em>Prevotella</em> species recovered from clinical samples showed that several members of this genus (8 out 10 isolates) were able to significantly reduce biofilm formation of <em>P. aeruginosa</em> PAO1, without impact on growth. Among the tested isolates, the strongest biofilm-inhibitory activity was observed for <em>Prevotella intermedia</em> and <em>Prevotella nigrescens</em>, which caused a reduction of up to 90% in the total biofilm biomass of several <em>P. aeruginosa</em> isolates from pwCF. In addition, a strain-specific effect of <em>P. nigrescens</em> on the ability of <em>P. aeruginosa</em> to produce proteases and pyocyanin was observed, with significant alterations in the levels of these virulence factors detected in LasR mutant strains. Overall, these results suggest that non-pathogenic bacteria from the lung microbiota may regulate pathogenicity traits of <em>P. aeruginosa</em>, and possibly affect the outcome of chronic lung infections.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100206"},"PeriodicalIF":6.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000315/pdfft?md5=53284d8ea73d7333e00152e9ef5d2a65&pid=1-s2.0-S2590207524000315-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress responses in biofilms","authors":"Waleska Stephanie da Cruz Nizer,&nbsp;Madison Elisabeth Adams,&nbsp;Kira Noelle Allison,&nbsp;Megan Catherine Montgomery,&nbsp;Hailey Mosher,&nbsp;Edana Cassol,&nbsp;Joerg Overhage","doi":"10.1016/j.bioflm.2024.100203","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100203","url":null,"abstract":"<div><p>Oxidizing agents are low-molecular-weight molecules that oxidize other substances by accepting electrons from them. They include reactive oxygen species (ROS), such as superoxide anions (O₂⁻), hydrogen peroxide (H₂O₂), and hydroxyl radicals (HO⁻), and reactive chlorine species (RCS) including sodium hypochlorite (NaOCl) and its active ingredient hypochlorous acid (HOCl), and chloramines. Bacteria encounter oxidizing agents in many different environments and from diverse sources. Among them, they can be produced endogenously by aerobic respiration or exogenously by the use of disinfectants and cleaning agents, as well as by the mammalian immune system. Furthermore, human activities like industrial effluent pollution, agricultural runoff, and environmental activities like volcanic eruptions and photosynthesis are also sources of oxidants. Despite their antimicrobial effects, bacteria have developed many mechanisms to resist the damage caused by these toxic molecules. Previous research has demonstrated that growing as a biofilm particularly enhances bacterial survival against oxidizing agents. This review aims to summarize the current knowledge on the resistance mechanisms employed by bacterial biofilms against ROS and RCS, focussing on the most important mechanisms, including the formation of biofilms in response to oxidative stressors, the biofilm matrix as a protective barrier, the importance of detoxifying enzymes, and increased protection within multi-species biofilm communities. Understanding the complexity of bacterial responses against oxidative stress will provide valuable insights for potential therapeutic interventions and biofilm control strategies in diverse bacterial species.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100203"},"PeriodicalIF":6.8,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000285/pdfft?md5=8b8a59489262c80363e8d06d2ebb6fbb&pid=1-s2.0-S2590207524000285-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141094948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}