Biofilm最新文献

{"title":"Novel PHL derivatives targeting quorum sensing: A strategy to combat Pseudomonas aeruginosa infections","authors":"Juanyan Liu ,&nbsp;Yi Deng ,&nbsp;Xinran Wang ,&nbsp;Piao Luo ,&nbsp;Shuaiwen Ding ,&nbsp;Weizhong Zhang ,&nbsp;Shahab Uddin ,&nbsp;Hongyu Li ,&nbsp;Yang Li","doi":"10.1016/j.bioflm.2025.100326","DOIUrl":"10.1016/j.bioflm.2025.100326","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) is a common pathogenic bacterium that is widely distributed and highly pathogenic. Its pathogenicity is closely related to the formation of biofilms and virulence factors. It is well known that quorum sensing (QS) controls the formation of biofilms and the production of virulence factors in <em>P. aeruginosa</em>. Therefore, quorum-sensing inhibitors (QSIs) have attracted great interest as a potential therapeutic strategy against <em>P. aeruginosa</em> infections. This study departs from the conventional approach of screening potential QSIs through experimental synthesis and subsequent activity validation, which is typically time-consuming and labor-intensive. Instead, we integrated molecular docking, molecular dynamics simulations, and experimental synthesis with validation strategies to systematically evaluate newly designed Phenylacetanoyl homoserine lactones (PHLs) derivatives. In the computational screening phase, the binding affinity, binding sites, and binding stability between the PHL derivatives and the target protein LasR were comprehensively assessed using molecular docking and molecular dynamics simulations. Five compounds demonstrating promising potential QS inhibitory activity were selected. During the experimental phase, these five compounds underwent preliminary evaluation for their anti-biofilm activity against <em>P. aeruginosa</em>. Results indicated that N-(3-cyclobutyl lactone)-4-nitrobenzobutyramide (<strong>L2</strong>) exhibited the most potent biofilm inhibitory activity. Compared to previously reported QSIs, <strong>L2</strong> effectively inhibited biofilm formation, toxin production, and motility of <em>P. aeruginosa</em> at lower concentrations. Notably, <strong>L2</strong> exhibited characteristics of a partial agonist, which sheds light on its deeper mechanism of action. Furthermore, <strong>L2</strong> showed a synergistic effect when combined with antibiotics, significantly enhancing the susceptibility of biofilm bacteria of <em>P. aeruginosa</em> to antibiotics, while demonstrating a favorable hemocompatibility safety profile. The proposed combination strategy offers a new potential therapeutic avenue for the clinical treatment of <em>P. aeruginosa</em> infections.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100326"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Legionella affects biofilm structural response to detachment upon shear stress increase","authors":"Ana Rosa Silva ,&nbsp;C. William Keevil ,&nbsp;Ana Pereira","doi":"10.1016/j.bioflm.2025.100323","DOIUrl":"10.1016/j.bioflm.2025.100323","url":null,"abstract":"<div><div>Poor hydraulic management at water systems is associated with an increased risk of Legionnaires' disease caused by <em>Legionella</em>. Stagnation periods, followed by sudden water flow, can promote biofilm detachment and the release of <em>Legionella</em> into the bulk water. Regardless of its importance, the simultaneous effects of shear stress on biofilm detachment and <em>Legionella</em> release into the bulk water remain poorly understood. This study investigates how shear stress affects biofilms containing <em>Legionella pneumophila</em> in terms of: a) biofilm detachment, b) release of <em>L. pneumophila</em> into the bulk phase, and c) shifting of <em>L. pneumophila</em> into the viable but nonculturable (VBNC) state. <em>Pseudomonas fluorescens</em> biofilms were formed in a Center for Disease Control (CDC) biofilm reactor at 125 RPM and spiked with <em>L. pneumophila</em>. After 6 days, the system was set for 48 h to stagnation before flow was resumed at rotational velocities of 125, 225, and 400 RPM, corresponding to turbulent regimes with Reynolds numbers of 1552, 2794 and 4966, respectively. Biofilm properties, <em>L. pneumophila</em> viability, culturability, and spatial distribution were monitored. Results show that biofilms containing <em>L. pneumophila</em> maintained a similar basal thickness (12 μm) despite the detachment of the upper layers under different shear stresses. <em>L. pneumophila</em>, located at the bottom of the biofilm, remains surface-attached after biofilm detachment and seems to enhance the cohesiveness of these layers compared to <em>P. fluorescens</em> biofilms. On the contrary, when <em>Legionella</em> is not present, biofilm detachment increases with the increase of applied shear forces. All tested rotational velocities triggered <em>L. pneumophila</em> to enter the VBNC state in the bulk phase, while biofilm-associated VBNC cells were only observed at 400 RPM.</div><div>Finally, the contribution of the present work to <em>Legionella</em> control practices in water systems is discussed, highlighting the important insights that biofilms can provide in this context.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100323"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaginal Lactobacillus gasseri biosurfactant: a novel bio- and eco-compatible anti-Candida agent","authors":"Federica Monti ,&nbsp;Barbara Giordani ,&nbsp;Stefano Fedi ,&nbsp;Daniele Ghezzi ,&nbsp;Paola Galletti ,&nbsp;Laura Mercolini ,&nbsp;Roberto Mandrioli ,&nbsp;Carola Parolin ,&nbsp;Barbara Luppi ,&nbsp;Beatrice Vitali","doi":"10.1016/j.bioflm.2025.100299","DOIUrl":"10.1016/j.bioflm.2025.100299","url":null,"abstract":"<div><div>Vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC), caused by <em>Candida</em> spp. overgrowth, are common and challenging infections of the genital tract. Among <em>Candida</em> virulence factors, the ability to adhere to host epithelium and to form biofilms are frequently retrieved, especially in RVVC. Vaginal pathogen overgrowth is counteracted by resident lactobacilli, which exert a barrier thanks to the production of antimicrobial metabolites, such as biosurfactants (BS).</div><div>BS was recovered from vaginal <em>Lactobacillus gasseri</em> BC12 and its chemical characteristics as well as its ability to lower the surface tension and to emulsify two different immiscible phases were investigated. BS showed the typical features of a lipopeptide with a critical micellar concentration of 1.2 mg/mL. BS showed antibiofilm activity towards various <em>Candida albicans</em> and non-<em>albicans</em> isolates, notably, it was able to prevent biofilm formation and eradicate preformed biofilms. The absence of cytotoxicity of BS and its ability to counteract the adhesion of <em>Candida</em> spp. were highlighted on HeLa cells through MTT and competition/exclusion assays, respectively. The environmental impact of BS was also investigated on a microcosm model (spring water) by culture-based and molecular (16S rRNA-targeted Illumina sequencing) methods, and no remarkable modifications in the taxonomy composition of the bacterial ecosystem were observed.</div><div>To conclude, BS from <em>L. gasseri</em> BC12 appears as a promising, biocompatible and environmentally friendly approach to prevent and treat VVC/RVVC.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100299"},"PeriodicalIF":5.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and metabolomic insights from functionalized V-shaped Lactiplantibacillus plantarum towards mitigating Candida albicans virulence","authors":"Satish Kumar Rajasekharan ,&nbsp;Athira Venugopal ,&nbsp;Hamitha Chinganadi Hameed ,&nbsp;Jennessa Jacob ,&nbsp;Vinothkannan Ravichandran ,&nbsp;Doron Steinberg ,&nbsp;Adi Faigenboim ,&nbsp;Chaitany Jayprakash Raorane ,&nbsp;Moshe Shemesh","doi":"10.1016/j.bioflm.2025.100321","DOIUrl":"10.1016/j.bioflm.2025.100321","url":null,"abstract":"<div><div>The genomic capacity of probiotic Lactobacilli enables producing bioactive metabolites that represent promising alternatives to antibiotics for combating food-borne and multi drug-resistant pathogens. The current study characterizes a global antagonistic potential of the probiotic bacterium <em>Lactiplantibacillus plantarum</em>, which demonstrates unique multicellular structuring triggered in response to acidic environments. We demonstrate the inhibitory potential of postbiotics derived from the V-shaped structured <em>L. plantarum</em> (VSLP) against <em>Candida albicans</em>. Using the liquid chromatography-mass spectrometry (LC-MS) profiling, we further identify different antibiofilm metabolites secreted during the VSLP formation. Transcriptomic studies reveal a notable upregulation of genes associated with the biosynthesis of aromatic amino acids such as <em>serD, ywqE2, trpB, trpA</em>. Furthermore, significant differential expressions were observed in genes within the biosynthetic gene cluster (BGCs) identified through antiSMASH analysis of <em>L. plantarum</em> genome. In addition, two novel VSLP metabolites (bluensomycin and majoroside F6) functioned as inhibitors of Ras-adenylate cyclase pathway that control biofilms and hyphae in yeast. We suggest that functionalized probiotic cells, such as VSLP, may effectively control the pathogenic microorganisms that provide a staple basis for developing future therapeutic probiotics.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100321"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A semi-solid in vitro biofilm model for evaluating antimicrobial potency and biofilm-specific activity","authors":"Albert Fuglsang-Madsen ,&nbsp;Lasse Andersson Kvich ,&nbsp;Nicole Lind Henriksen ,&nbsp;Rasmus Kristensen ,&nbsp;Jonas Rosager Henriksen ,&nbsp;Anders Elias Hansen ,&nbsp;Thomas Bjarnsholt ,&nbsp;Tim Holm Jakobsen","doi":"10.1016/j.bioflm.2025.100328","DOIUrl":"10.1016/j.bioflm.2025.100328","url":null,"abstract":"<div><div>Biofilms play a critical role in chronic bacterial infections, and new potent antimicrobials are urgently needed to address the escalating problem of antimicrobial resistance to existing therapies. To support the development of such therapeutics, there is a pressing need for biofilm models that better recapitulate the microenvironment of <em>in vivo</em> conditions. Existing <em>in vitro</em> assays, such as the widely used minimum biofilm eradication concentration (MBEC) assay, rely on liquid cultures that poorly reflect the structural and physiological characteristics of tissue-associated biofilms.</div><div>To address these limitations, we developed the Modified Crone's Model (MCM), a reproducible, semi-solid biofilm model that embeds bacteria in soft-tissue-like agar-based matrices. We established the MCM as a platform for evaluating the antimicrobial and specific anti-biofilm activity of novel and existing compounds. Biofilms grown in the MCM displayed consistent growth, <em>in vivo</em>-like morphology, and reduced variability compared to liquid-culture systems. Notably, antimicrobial susceptibility rankings in the MCM differed substantially from traditional assays, emphasizing that model-specific conditions can markedly affect the evaluation of antimicrobial potency and should be considered when selecting biofilm test systems.</div><div>Using the MCM, we screened a panel of therapeutic agents and identified two unsaturated fatty acids – <em>cis</em>-2-decenoic acid and <em>cis</em>-11-methyl-2-dodecenoic acid – as potent antibiotic potentiators with intrinsic anti-biofilm activity, undetectable in microbroth dilution assays. We further demonstrated the MCM's adaptability by replicating susceptibility profiles in biofilms grown on porcine bone tissue and implant surfaces, with no significant differences from agar-based biofilms.</div><div>The MCM offers a simple and reproducible platform for preclinical antimicrobial screening under semi-solid growth conditions that better reflect the spatial and diffusional constraints of biofilm-associated infections.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100328"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145578633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental plaque biofilm transforms host-derived β2-microglobulin into polymorphic fibrils for integration into the biofilm matrix","authors":"Taiki Mori,&nbsp;Eisuke Domae,&nbsp;Mariko Hanaoka,&nbsp;Takeshi Into","doi":"10.1016/j.bioflm.2025.100331","DOIUrl":"10.1016/j.bioflm.2025.100331","url":null,"abstract":"<div><div>Dental plaque is a polymicrobial biofilm formed on tooth surfaces despite continuous exposure to variable host-derived antimicrobial factors. To date, the underlying mechanisms remain nebulous. This study aimed to determine whether dental plaque biofilms affect the major salivary antimicrobial protein β₂-microglobulin (B2M). Immunostaining of human dental plaque specimens with an anti-B2M antibody revealed that B2M exists as elongated fibers, punctate structures, and amorphous aggregates. Fractionation of dental plaque suspensions revealed that B2M was present in both the soluble and insoluble fractions. B2M, which forms insoluble fibrils associated with dialysis-related amyloidosis, exhibited comparable fibril-forming properties in dental plaque. Immunostaining with a developed anti-B2M amyloid fibril antibody showed that fibrillar B2Ms (fB2Ms) were distributed throughout the dental plaque specimens. <em>In vitro</em> experiments using purified B2M demonstrated that environmental factors characteristic of dental plaque, specifically phosphate ions, bacterial short-chain fatty acids (acetic, butyric, lactic, and propionic acids), and divalent calcium and magnesium ions, significantly promoted fB2M formation. In a <em>Streptococcus mutans</em> biofilm model, native B2M transformed into fibrils only in the presence of these environmental factors, resulting in the loss of its antimicrobial activity and its incorporation into the biofilm matrix. The preformed fB2Ms increased <em>S. mutans</em> biofilm growth, decreased biofilm adhesion, and transformed the biofilm matrix architecture from a membranous to a reticulated organization, potentially facilitating biofilm dissemination. Dental plaque biofilms employed a specialized “molecular hijacking” strategy to counteract host defense mechanisms and ensure persistence through fibrillation. Our findings provide novel insights into biofilm pathogenesis, host-microbe interactions, and potential plaque control approaches.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100331"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145578632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ambroxol hydrochloride as an antibiofilm agent synergizes with tetracycline antibiotics against mature biofilms of multidrug-resistant Klebsiella pneumoniae","authors":"TengLi Zhang ,&nbsp;XunQin Gao ,&nbsp;MengTing Liu ,&nbsp;Chun Wen ,&nbsp;Peng Jin ,&nbsp;Hong Yao ,&nbsp;XiWang Liu ,&nbsp;YingLan Yu ,&nbsp;Hao Shao ,&nbsp;Lei Luo","doi":"10.1016/j.bioflm.2025.100315","DOIUrl":"10.1016/j.bioflm.2025.100315","url":null,"abstract":"<div><div>Multidrug-resistant <em>Klebsiella pneumoniae</em> (MDR-KP) is a major pathogen responsible for hospital-acquired infections, associated with high morbidity and mortality. Biofilm formation plays a key role in the pathogenicity of MDR-KP and contributes significantly to its antibiotic resistance, substantially impairing the effectiveness of antimicrobial therapies. To enhance the efficacy of existing antibiotics, this study investigates a biofilm-targeting synergistic strategy inspired by the structural similarity between sputum and biofilm matrices. In this study, 87 clinical isolates of MDR-KP were initially screened for biofilm-forming capacity, and strong biofilm producers were selected to establish an <em>in vitro</em> model for systematic evaluation of the anti-biofilm efficacy of six mucolytic agents. Ambroxol hydrochloride (ABH) emerges as the optimal effective, disrupting biofilm structure at 0.7 mg/mL and achieving 50 % clearance within 8 h. ABH enhanced the anti-biofilm activity of tetracycline and doxycycline <em>in vitro</em>, reducing their IC₅₀ values by 98.9 % and 98.6 %, respectively, against preformed biofilms of MDR-KP compared to monotherapy. Additionally, the excellent physical and chemical compatibility between ABH and tetracycline or doxycycline provides a stable basis for <em>in vivo</em> co-administration. <em>In vivo</em>, the combination alleviates pulmonary inflammation, reduces bacterial load and inflammatory factor levels, and shows no tissue toxicity. In conclusion, ABH combined with tetracycline antimicrobials enhanced their efficacy against MDR-KP infections, especially biofilm-associated infections, in both <em>in vitro</em> and <em>in vivo</em> models, and possessed a favorable physicochemical compatibility and safety profile. These findings suggested that ABH-tetracycline therapy could represent a translationally promising and effective strategy for combating clinical MDR-KP infections.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100315"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual red and near-infrared LED therapy inhibits MRSA biofilm in otitis media","authors":"Yoo-Seung Ko,&nbsp;Eun-Ji Gi,&nbsp;Sungsu Lee,&nbsp;Hong-Chan Kim,&nbsp;Hyong-Ho Cho","doi":"10.1016/j.bioflm.2025.100314","DOIUrl":"10.1016/j.bioflm.2025.100314","url":null,"abstract":"<div><div>Otitis media (OM), particularly when caused by methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), can become refractory due to biofilm formation, which contributes to resistance against conventional antimicrobial treatments. Photobiomodulation using light-emitting diode (LED) therapy has recently emerged as a promising non-antibiotic strategy for managing refractory infections by targeting biofilm-associated pathology. However, especially in the context of MRSA-induced OM, its therapeutic efficacy and underlying mechanisms remain incompletely elucidated. In this study, we established a rat model of OM by inoculating MRSA (5 × 10⁸ CFUs) into the middle ear via the tympanic membrane. Red and near-infrared (NIR) LED irradiation (655/842 nm; 163.2 W/m²; 30 min/day for 5 days) was administered 1 week after infection. Scanning electron microscopy revealed a marked reduction in MRSA biofilm structures, and biofilm biomass was significantly decreased, as assessed by crystal violet staining. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis demonstrated significant downregulation of <em>fib</em>, <em>icaB</em>, <em>icaC</em>, and <em>icaD</em>, key genes crucial for bacterial adhesion and biofilm development. Histological assessment further showed decreased mucosal thickening and macrophage infiltration, supported by reduced ionized calcium-binding adapter molecule 1 (Iba1) expression. These findings suggest that dual red and NIR LED therapy effectively suppresses MRSA biofilm formation and inflammation in OM, indicating its potential as a novel non-antibiotic therapy for biofilm-associated OM that may help manage persistent or treatment-resistant cases in clinical settings.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100314"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse microbial communities colonize biofilm carriers in moving bed and intermittent cleaning reactors for municipal and industrial wastewater treatment","authors":"Eshetu Janka ,&nbsp;Ram Prasath Alagappan ,&nbsp;Dipaluk Das ,&nbsp;Leif Arne Kjeldsberg ,&nbsp;Shuai Wang ,&nbsp;Tone Haugen ,&nbsp;Alexander Wentzel","doi":"10.1016/j.bioflm.2025.100337","DOIUrl":"10.1016/j.bioflm.2025.100337","url":null,"abstract":"<div><div>Conventional biofilm reactors are widely used for the removal of organic constituents and nutrients (i.e., nitrogen and phosphorus) in municipal and industrial wastewater treatment. Moving bed biofilm reactor (MBBR) and continuously flow intermittent cleaning reactor (CFIC) have been developed as more compact, small footprint, and highly efficient biofilm-based systems for wastewater treatment. However, despite the advancements in reactor technology, there is limited scientific information on the microbial composition of the biofilms in these systems. This study aimed to characterize and provide early insights into the microbial diversity of biofilms grown on biofilm carriers and liquid suspensions of the biofilm-based wastewater treatment systems. Microbial samples were collected from biofilm carriers and liquid suspension in four full-scale MBBR plants and two CFIC plants in Norway, all treating municipal and industrial wastewater. DNA was extracted from the samples and subjected to <em>meta</em>-barcode sequencing for taxonomic classification of microbial communities in each treatment plant. The results revealed significant variation in microbial compositions across treatment plants, influenced by wastewater characteristics, biofilm carrier types, and reactor operational characteristics. On the biofilm carriers, the dominant bacterial taxa included TM7-1 (<em>Saccharibacteria</em>), <em>Burkholderiales</em>, <em>Clostridiales</em>, <em>Actinomycetales</em>, <em>Pseudomonadales</em>, <em>Rickettsiales</em>, and <em>Rhodobacteriales</em>. In liquid suspensions, the dominant groups were <em>Clostridiales</em>, <em>Methanosarcinales</em>, <em>Pseudomonadales</em>, <em>Flavobacteriales</em>, and <em>Rhodobacteriales</em>. In conclusion, this study highlights the diverse microbial populations on biofilm carriers and liquid suspensions, which collectively contribute to the enhanced treatment efficiency of both MBBR and CFIC systems.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100337"},"PeriodicalIF":4.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145617921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus","authors":"Hiren Dodia ,&nbsp;Suvendu Ojha ,&nbsp;Puja Chatterjee ,&nbsp;Tushar Kant Beuria","doi":"10.1016/j.bioflm.2025.100307","DOIUrl":"10.1016/j.bioflm.2025.100307","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) is a global concern that undermines microbial disease treatment and prevention. WHO and World Bank's EcoAMR report predicts that AMR could cause 39 million deaths and $3.4 trillion in annual GDP losses by the year 2050. This is particularly critical with <em>S. aureus</em>, a cause of diverse infections like skin abscesses and pneumonia, where antibiotic resistance increases mortality and hinders treatment. Biofilms are one of the major causes of multi-drug resistance in <em>S. aureus,</em> and their inhibition can restore antibiotic sensitivity. In this study, through screening of the LOPAC drug library, we identified several compounds that exhibit biofilm inhibitory properties against multi-drug-resistant <em>S. aureus</em> without affecting its growth. The compound 10058-F4 was found to have the strongest <em>anti</em>-biofilm activity (&gt;70 % inhibition) with minimal antibacterial effects (MIC 256 μg/mL); however, it showed no inhibitory effects on pre-existing biofilm. Further, the 10058-F4 treatment suppressed the expression of <em>sarA,</em> the biofilm master regulator, along with biofilm genes, such as <em>icaA, fnb, nuc, and sspA</em>. Additionally, the results showed that 10058-F4 synergistically enhanced the antibacterial activity of norfloxacin and tetracycline, indicating its potential use as an adjunct to the existing antibiotic treatments. While these findings suggest the potential of 10058-F4 for clinical use, further investigations are necessary to elucidate its mechanism of action and optimize its application in combination therapies.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100307"},"PeriodicalIF":5.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}